Rroxscaffold_2G00142450

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005515
Physical Location & Seq
Forward (+)
80515278 .. 80517189
1912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00142450.1

Sequence Viewer

Length: 1221 bp
ATGGCTGATGAGGCTGGTTCATCATCAAGTACTAGAGCTCATGGCAAGAGACTATGGTGTATGATGCCTGAACGTGCCCGGATTCACATGGCATTGACTGCCCTGCAGTTCAGTTATGCTGGGCAACATGTCATCCTCAGAACTGTGCTTAACATGGGTGTAAGCAAGCTTGTGTTCCCAGTTTACAGGAACATCATTGCGCTGCTTCTGCTCGGTCCCTTTGCATACTTTCTAGAGAAGAAAGATAGACCACCACTAAATTTTGGCCTTGTCATGCAGTTTTTCCTCATGGGACTTCTTGGGATCACATGCAATCAAGGATTGTACCTCTTGGGTTTGGAAAACACTTCGCCAACATTTGCTTCTGCAATAGAGAATGCAGTCCCTGCAATTACATTTTTCATGGCTGCACTATTCAGGCAAATATTGTTCATCGGACTTGTAGCAATTCGTGCTAGAGCATTTGTATATGCTTCTGTAGGAAGCGTTTTAATTGAACAAGTTCACTTGAATAGAAAAGATGGCAAAGCCAAGGTGCTTGGAACTCTGGCTTCTGTTGCTGGAGCCTCAGTCATAACCATTTACAAAGGGCCAACAATTTATGGACCCCCAAGTGATTCACAATATTTAAACCAATCTCATTCTCTGTTCTCATCATTTGGAGATGCCAAGGAGAAGAATTGGACCTTGGGATGCATCTGTCTAATTGGTCATTGCCTGTGTTGGTCTAGCTGGATTGTGCTACAAGCACCGGTTTTGAAGAAATACCCAGCTCGCCTTTCAGTCACCTCATTTACTTGCTTCTTTGGCATTCTGCAATGTTCTGCAATCGCGGGGATTGTAGAGAGAGATTCCCAAGCCTGGCAGGTCCATTCTGGTGGTGAAGTTGTTGCTATTCTCTACGCGGGAGTGGTGGCTTCAGCAATGGCATTTGCATTACAGATATGGGTGATTGAACGAGGAGGCCCAGTGTTCGTTTCAGTGTATCTGCCTCTACAGACATTACTGGTAGCTCTAATGGCCTCAGTCATTTTAGGTGAACAATTCTACTTGGGAGGCATTATTGGAGCAGTATTGATCGTAGCCGGACTCTACCTGGTGGTCTGGGGGAAAAATGAAGAGAGCAAGTTTGGCAATGAAAATGGTGAATTTCCTCGATCAATGCCTGCAGATGAACAATTCTCTCTCTTTCAGTCATTACTCAGTTCCTCTTCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

406

Amino Acids

44.18

Weight (kDa)

8.94

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 36 - 139 3.2e-12 EamA-like transporter family
EamA PF00892 230 - 368 4.4e-17 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 856
AccII CGCG 2 cut(s) 833, 905
AciI CCGC 2 cut(s) 833, 905
AclWI GGATC 1 cut(s) 311
AcsI RAATTY 2 cut(s) 259, 1148
AcuI CTGAAG 1 cut(s) 903
AfaI GTAC 2 cut(s) 31, 326
AfiI CCNNNNNNNGG 1 cut(s) 861
AflIII ACRYGT 1 cut(s) 127
AgeI ACCGGT 1 cut(s) 751
AgsI TTSAA 4 cut(s) 497, 511, 760, 956
AjnI CCWGG 2 cut(s) 860, 1095
AjuI GAANNNNNNNTTGG 4 cut(s) 346, 378, 671, 703
AluBI AGCT 5 cut(s) 38, 169, 732, 773, 1013
AluI AGCT 5 cut(s) 38, 169, 732, 773, 1013
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 1 cut(s) 43
AlwI GGATC 1 cut(s) 311
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 4 cut(s) 265, 590, 964, 1020
ApeKI GCWGC 2 cut(s) 202, 407
ApoI RAATTY 2 cut(s) 259, 1148
AsiGI ACCGGT 1 cut(s) 751
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 6 cut(s) 215, 590, 605, 684, 868, 965
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 5 cut(s) 778, 893, 961, 1049, 1157
AsuII TTCGAA 1 cut(s) 1214
AvaII GGWCC 4 cut(s) 215, 605, 684, 868
BaeGI GKGCMC 1 cut(s) 79
BanII GRGCYC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 2 cut(s) 189, 394
BccI CCATC 1 cut(s) 515
BciT130I CCWGG 2 cut(s) 862, 1097
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 4 cut(s) 33, 233, 456, 729
BfmI CTRYAG 4 cut(s) 104, 477, 995, 1167
BfuAI ACCTGC 1 cut(s) 856
BisI GCNGC 2 cut(s) 203, 408
BlsI GCNGC 2 cut(s) 204, 409
BmcAI AGTACT 1 cut(s) 31
Bme1390I CCNGG 3 cut(s) 79, 862, 1097
Bme18I GGWCC 4 cut(s) 215, 605, 684, 868
BmgT120I GGNCC 6 cut(s) 215, 590, 605, 684, 868, 965
BmiI GGNNCC 3 cut(s) 217, 565, 607
BmrFI CCNGG 3 cut(s) 79, 862, 1097
BmrI ACTGGG 2 cut(s) 173, 962
BmsI GCATC 4 cut(s) 54, 655, 683, 705
BmuI ACTGGG 2 cut(s) 173, 962
BpmI CTGGAG 1 cut(s) 582
Bpu14I TTCGAA 1 cut(s) 1214
BpuMI CCSGG 1 cut(s) 79
BsaJI CCNNGG 3 cut(s) 531, 669, 687
BsaWI WCCGGW 1 cut(s) 751
BsaXI ACNNNNNCTCC 4 cut(s) 555, 585, 954, 984
Bsc4I CCNNNNNNNGG 1 cut(s) 861
Bse118I RCCGGY 1 cut(s) 751
Bse1I ACTGG 3 cut(s) 179, 968, 1011
Bse3DI GCAATG 5 cut(s) 195, 712, 824, 930, 1141
BseBI CCWGG 2 cut(s) 862, 1097
BseDI CCNNGG 3 cut(s) 531, 669, 687
BseGI GGATG 2 cut(s) 132, 698
BseLI CCNNNNNNNGG 1 cut(s) 861
BseMI GCAATG 5 cut(s) 195, 712, 824, 930, 1141
BseMII CTCAG 4 cut(s) 151, 582, 1038, 1216
BseNI ACTGG 3 cut(s) 179, 968, 1011
BseRI GAGGAG 1 cut(s) 975
BseSI GKGCMC 1 cut(s) 79
BseXI GCAGC 2 cut(s) 189, 394
BseYI CCCAGC 2 cut(s) 119, 769
BsgI GTGCAG 1 cut(s) 393
Bsh1236I CGCG 2 cut(s) 833, 905
BshFI GGCC 4 cut(s) 267, 592, 966, 1022
BshTI ACCGGT 1 cut(s) 751
BsiHKAI GWGCWC 1 cut(s) 40
BsiSI CCGG 3 cut(s) 79, 752, 1086
BslFI GGGAC 3 cut(s) 201, 306, 368
BslI CCNNNNNNNGG 1 cut(s) 861
BsmAI GTCTC 1 cut(s) 43
BsmFI GGGAC 3 cut(s) 201, 306, 368
BsmI GAATGC 2 cut(s) 382, 810
BsnI GGCC 4 cut(s) 267, 592, 966, 1022
Bsp119I TTCGAA 1 cut(s) 1214
Bsp1286I GDGCHC 2 cut(s) 40, 79
Bsp143I GATC 3 cut(s) 303, 1077, 1157
BspACI CCGC 2 cut(s) 833, 905
BspANI GGCC 4 cut(s) 267, 592, 966, 1022
BspCNI CTCAG 4 cut(s) 150, 581, 1037, 1215
BspFNI CGCG 2 cut(s) 833, 905
BspLI GGNNCC 3 cut(s) 217, 565, 607
BspMAI CTGCAG 2 cut(s) 108, 1171
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 1 cut(s) 311
BspT104I TTCGAA 1 cut(s) 1214
BsrDI GCAATG 5 cut(s) 195, 712, 824, 930, 1141
BsrFI RCCGGY 1 cut(s) 751
BsrI ACTGG 3 cut(s) 179, 968, 1011
BssAI RCCGGY 1 cut(s) 751
BssECI CCNNGG 3 cut(s) 531, 669, 687
BssMI GATC 3 cut(s) 303, 1077, 1157
BssT1I CCWWGG 3 cut(s) 531, 669, 687
Bst2UI CCWGG 2 cut(s) 862, 1097
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 2 cut(s) 1113, 1216
BstAPI GCANNNNNTGC 3 cut(s) 98, 386, 452
BstBI TTCGAA 1 cut(s) 1214
BstC8I GCNNGC 3 cut(s) 167, 775, 1167
BstDEI CTNAG 4 cut(s) 137, 568, 1024, 1202
BstF5I GGATG 2 cut(s) 132, 698
BstFNI CGCG 2 cut(s) 833, 905
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 3 cut(s) 306, 1080, 1160
BstMAI GTCTC 1 cut(s) 43
BstMBI GATC 3 cut(s) 303, 1077, 1157
BstMWI GCNNNNNNNGC 9 cut(s) 11, 98, 208, 386, 452, 557, 807, 1019, 1131
BstNI CCWGG 2 cut(s) 862, 1097
BstNSI RCATGY 2 cut(s) 131, 312
BstSCI CCNGG 3 cut(s) 77, 860, 1095
BstSFI CTRYAG 4 cut(s) 104, 477, 995, 1167
BstSLI GKGCMC 1 cut(s) 79
BstUI CGCG 2 cut(s) 833, 905
BstV1I GCAGC 2 cut(s) 189, 394
BstXI CCANNNNNNTGG 1 cut(s) 878
BsuRI GGCC 4 cut(s) 267, 592, 966, 1022
BtsCI GGATG 2 cut(s) 132, 698
BtsIMutI CAGTG 2 cut(s) 975, 987
BveI ACCTGC 1 cut(s) 856
Cac8I GCNNGC 3 cut(s) 167, 775, 1167
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 751
Cfr13I GGNCC 6 cut(s) 215, 590, 605, 684, 868, 965
CsiI ACCWGGT 1 cut(s) 1095
Csp6I GTAC 2 cut(s) 30, 325
CspAI ACCGGT 1 cut(s) 751
CviAII CATG 8 cut(s) 41, 88, 128, 154, 274, 289, 309, 403
CviQI GTAC 2 cut(s) 30, 325
DdeI CTNAG 4 cut(s) 137, 568, 1024, 1202
DpnI GATC 3 cut(s) 305, 1079, 1159
DpnII GATC 3 cut(s) 303, 1077, 1157
DraI TTTAAA 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 1113, 1216
EarI CTCTTC 2 cut(s) 1113, 1216
Ecl136II GAGCTC 1 cut(s) 38
Eco130I CCWWGG 3 cut(s) 531, 669, 687
Eco24I GRGCYC 1 cut(s) 40
Eco47I GGWCC 4 cut(s) 215, 605, 684, 868
Eco53kI GAGCTC 1 cut(s) 38
Eco57I CTGAAG 1 cut(s) 903
EcoICRI GAGCTC 1 cut(s) 38
EcoRII CCWGG 2 cut(s) 860, 1095
EcoT14I CCWWGG 3 cut(s) 531, 669, 687
EcoT22I ATGCAT 1 cut(s) 698
EcoT38I GRGCYC 1 cut(s) 40
ErhI CCWWGG 3 cut(s) 531, 669, 687
FaeI CATG 8 cut(s) 44, 91, 131, 157, 277, 292, 312, 406
FaqI GGGAC 3 cut(s) 201, 306, 368
FatI CATG 8 cut(s) 40, 87, 127, 153, 273, 288, 308, 402
FauI CCCGC 2 cut(s) 826, 898
Fnu4HI GCNGC 2 cut(s) 203, 408
FokI GGATG 2 cut(s) 119, 705
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 2 cut(s) 203, 408
FspBI CTAG 4 cut(s) 33, 233, 456, 729
GlaI GCGC 1 cut(s) 201
GluI GCNGC 2 cut(s) 203, 408
GsaI CCCAGC 2 cut(s) 123, 773
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 4 cut(s) 267, 592, 966, 1022
HapII CCGG 3 cut(s) 79, 752, 1086
HhaI GCGC 1 cut(s) 202
Hin1II CATG 8 cut(s) 44, 91, 131, 157, 277, 292, 312, 406
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 4 cut(s) 82, 617, 851, 1089
HpaII CCGG 3 cut(s) 79, 752, 1086
HphI GGTGA 5 cut(s) 778, 893, 961, 1049, 1157
Hpy166II GTNNAC 3 cut(s) 184, 505, 1040
Hpy188I TCNGA 2 cut(s) 140, 437
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 3 cut(s) 184, 505, 1040
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 73
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 98, 208, 386, 452, 557, 807, 1019, 1131
HpyF3I CTNAG 4 cut(s) 137, 568, 1024, 1202
HpySE526I ACGT 1 cut(s) 73
Hsp92II CATG 8 cut(s) 44, 91, 131, 157, 277, 292, 312, 406
HspAI GCGC 1 cut(s) 200
Kzo9I GATC 3 cut(s) 303, 1077, 1157
LmnI GCTCC 2 cut(s) 563, 1067
Lsp1109I GCAGC 2 cut(s) 189, 394
LweI GCATC 4 cut(s) 54, 655, 683, 705
MabI ACCWGGT 1 cut(s) 1095
MaeI CTAG 4 cut(s) 33, 233, 456, 729
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 1 cut(s) 784
MalI GATC 3 cut(s) 305, 1079, 1159
MboI GATC 3 cut(s) 303, 1077, 1157
MboII GAAGA 5 cut(s) 250, 688, 772, 1130, 1203
MhlI GDGCHC 2 cut(s) 40, 79
MlyI GAGTC 1 cut(s) 1083
Mph1103I ATGCAT 1 cut(s) 698
MroXI GAANNNNTTC 1 cut(s) 501
MseI TTAA 3 cut(s) 150, 491, 629
MslI CAYNNNNRTG 1 cut(s) 876
MspI CCGG 3 cut(s) 79, 752, 1086
MspR9I CCNGG 3 cut(s) 79, 862, 1097
Mva1269I GAATGC 2 cut(s) 382, 810
MvaI CCWGG 2 cut(s) 862, 1097
MvnI CGCG 2 cut(s) 833, 905
MwoI GCNNNNNNNGC 9 cut(s) 11, 98, 208, 386, 452, 557, 807, 1019, 1131
NciI CCSGG 1 cut(s) 79
NdeII GATC 3 cut(s) 303, 1077, 1157
NlaIII CATG 8 cut(s) 44, 91, 131, 157, 277, 292, 312, 406
NlaIV GGNNCC 3 cut(s) 217, 565, 607
NmuCI GTSAC 1 cut(s) 784
NsiI ATGCAT 1 cut(s) 698
NspI RCATGY 2 cut(s) 131, 312
NspV TTCGAA 1 cut(s) 1214
PciI ACATGT 1 cut(s) 127
PctI GAATGC 2 cut(s) 382, 810
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 3 cut(s) 82, 617, 851
PinAI ACCGGT 1 cut(s) 751
PkrI GCNGC 2 cut(s) 204, 409
PleI GAGTC 1 cut(s) 1083
PpsI GAGTC 1 cut(s) 1083
PscI ACATGT 1 cut(s) 127
Psp124BI GAGCTC 1 cut(s) 40
Psp6I CCWGG 2 cut(s) 860, 1095
PspFI CCCAGC 2 cut(s) 119, 769
PspGI CCWGG 2 cut(s) 860, 1095
PspN4I GGNNCC 3 cut(s) 217, 565, 607
PspPI GGNCC 6 cut(s) 215, 590, 605, 684, 868, 965
PstI CTGCAG 2 cut(s) 108, 1171
PstNI CAGNNNCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 31, 326
RsaNI GTAC 2 cut(s) 30, 325
RseI CAYNNNNRTG 1 cut(s) 876
SacI GAGCTC 1 cut(s) 40
SaqAI TTAA 3 cut(s) 150, 491, 629
SatI GCNGC 2 cut(s) 203, 408
Sau3AI GATC 3 cut(s) 303, 1077, 1157
Sau96I GGNCC 6 cut(s) 215, 590, 605, 684, 868, 965
ScaI AGTACT 1 cut(s) 31
SchI GAGTC 1 cut(s) 1083
ScrFI CCNGG 3 cut(s) 79, 862, 1097
SduI GDGCHC 2 cut(s) 40, 79
SexAI ACCWGGT 1 cut(s) 1095
SfaNI GCATC 4 cut(s) 54, 655, 683, 705
SfcI CTRYAG 4 cut(s) 104, 477, 995, 1167
SfuI TTCGAA 1 cut(s) 1214
SinI GGWCC 4 cut(s) 215, 605, 684, 868
SmiMI CAYNNNNRTG 1 cut(s) 876
SsiI CCGC 2 cut(s) 833, 905
SspI AATATT 2 cut(s) 426, 626
SspMI CTAG 4 cut(s) 33, 233, 456, 729
SstI GAGCTC 1 cut(s) 40
StyD4I CCNGG 3 cut(s) 77, 860, 1095
StyI CCWWGG 3 cut(s) 531, 669, 687
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 76
TaqI TCGA 2 cut(s) 1156, 1214
TaqII GACCGA 1 cut(s) 203
TatI WGTACW 1 cut(s) 29
TfiI GAWTC 3 cut(s) 82, 617, 851
Tru1I TTAA 3 cut(s) 150, 491, 629
Tru9I TTAA 3 cut(s) 150, 491, 629
TscAI CASTG 2 cut(s) 975, 987
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 2 cut(s) 202, 407
Tsp45I GTSAC 1 cut(s) 784
TspDTI ATGAA 6 cut(s) 9, 391, 421, 1131, 1152, 1188
TspRI CASTG 2 cut(s) 975, 987
VpaK11BI GGWCC 4 cut(s) 215, 605, 684, 868
XapI RAATTY 2 cut(s) 259, 1148
XbaI TCTAGA 1 cut(s) 232
XceI RCATGY 2 cut(s) 131, 312
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 4 cut(s) 33, 233, 456, 729
ZrmI AGTACT 1 cut(s) 31
Zsp2I ATGCAT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.