Rw2G010590

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005515
Physical Location & Seq
Reverse (-)
10735153 .. 10737075
1923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010590.1

Sequence Viewer

Length: 1059 bp
ATGGCTGATTTATCATCAAGTGTAGCTAATAAGAGAAGATTGTGTATAGTGCCTGAAAATGCAAGGATTCATATGGCTTCAACTGTCCTTCAGTTCATCTATGCAGGGGTACATATCATTTGCAGAACTATGCTTAATATGAGTGTAAGCATGCTTGTTTTCTCAGTTTACAGGAATGTCACGGCGCTGCTTCTGCTGATTCCATTTGCATACTTTTTGGAGAAGAAAGATAGACCACCACTGAATATTTCCCTTGTCATGAAGTTTTTTCTCCTAGGACTTCTTGGGGTGACATGCAATCAAGGCTTGTATCTCTTTGGTTTAAAGAACACCTCCCCCACATTTGTTTCGACAATAGAGAATGCAGTCCCTGCAATAACATTTCTCATGGCTGCCTTATTCAGAATTGAACAAATGCACTTGAATAGAAAATATGGTAAAGCCAAAGTGCTTGGAACTCTTGCTTCTGTTGCTGGAGCCTCAGTCATAACCTTTTACAAGGGGCCAACTATTTATGTTCCTCCAAGTACTTCACCTTTAAGCCAATCCCATATGCTCAACTCATTATTTGGAGATGTCAAGGAGGAGAATTGGACCTTTGGTTACATCTGTGTAATAACACCTGTTCTGAAGAAATACCCAGCTCGCCTCTCAGTCACCTCATTTACTCTCTTCTTTGGCATTTTGCAACTTTTAGCAATCGTGGTGTGTGTAGAGAGAGATTCCCAAGCCTGGCAGTTCCATTACGGCAGTGAAGTCTTTGCCATTCTCTACATGGGAGGACCAGTGTATGTTTCACTGTATCTGCCTTTACAGACGGCAGTTGTAGCTGTAATGGACTCGCTCGTTTTTGGTGAAGAATTCTACTTGGGAAGCATTATTGGAGCAATGTTGACCATAGCAGGGCTGTACCTGGTGGTCTGGGGAAAAAGGCAGGAGAGCAAAATCAAGGTGGAAACTTCCTCAATGCCAGGGAACATTAATAGTCTGATAAACTATTTTTCTGGGGGAGAAGAACCCTCTCTTGTTCAAGCATTGCTCCCTACCTCTGCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

352

Amino Acids

38.89

Weight (kDa)

9.06

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 32 - 164 1.5e-15 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 860
AcuI CTGAAG 2 cut(s) 74, 650
AfaI GTAC 3 cut(s) 111, 529, 911
AfiI CCNNNNNNNGG 2 cut(s) 732, 903
AgsI TTSAA 4 cut(s) 81, 410, 424, 1031
AjnI CCWGG 3 cut(s) 731, 912, 970
AluBI AGCT 3 cut(s) 26, 644, 830
AluI AGCT 3 cut(s) 26, 644, 830
AlwNI CAGNNNCTG 1 cut(s) 371
AoxI GGCC 1 cut(s) 503
ApeKI GCWGC 2 cut(s) 187, 392
ApoI RAATTY 1 cut(s) 860
AseI ATTAAT 1 cut(s) 981
AspA2I CCTAGG 1 cut(s) 274
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 3 cut(s) 503, 594, 782
AsuHPI GGTGA 4 cut(s) 301, 525, 649, 866
AvaII GGWCC 2 cut(s) 594, 782
AvrII CCTAGG 1 cut(s) 274
BbvI GCAGC 2 cut(s) 174, 379
BceAI ACGGC 3 cut(s) 198, 763, 834
BciT130I CCWGG 3 cut(s) 733, 914, 972
BfaI CTAG 2 cut(s) 275, 1057
BfoI RGCGCY 1 cut(s) 188
BisI GCNGC 2 cut(s) 188, 393
BlnI CCTAGG 1 cut(s) 274
BlsI GCNGC 2 cut(s) 189, 394
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 3 cut(s) 733, 914, 972
Bme18I GGWCC 2 cut(s) 594, 782
BmgT120I GGNCC 3 cut(s) 503, 594, 782
BmiI GGNNCC 2 cut(s) 478, 504
BmrFI CCNGG 3 cut(s) 733, 914, 972
BpmI CTGGAG 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 274, 971
BsaXI ACNNNNNCTCC 4 cut(s) 468, 498, 771, 801
Bsc4I CCNNNNNNNGG 2 cut(s) 732, 903
Bse1I ACTGG 1 cut(s) 785
Bse3DI GCAATG 2 cut(s) 894, 1034
BseBI CCWGG 3 cut(s) 733, 914, 972
BseDI CCNNGG 2 cut(s) 274, 971
BseLI CCNNNNNNNGG 2 cut(s) 732, 903
BseMI GCAATG 2 cut(s) 894, 1034
BseMII CTCAG 3 cut(s) 177, 495, 666
BseNI ACTGG 1 cut(s) 785
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 2 cut(s) 174, 379
BseYI CCCAGC 1 cut(s) 640
BshFI GGCC 1 cut(s) 505
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 2 cut(s) 732, 903
BsmFI GGGAC 1 cut(s) 353
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 1 cut(s) 505
BspANI GGCC 1 cut(s) 505
BspCNI CTCAG 3 cut(s) 176, 494, 665
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 2 cut(s) 478, 504
BsrDI GCAATG 2 cut(s) 894, 1034
BsrI ACTGG 1 cut(s) 785
BssECI CCNNGG 2 cut(s) 274, 971
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 3 cut(s) 733, 914, 972
Bst4CI ACNGT 2 cut(s) 85, 801
Bst6I CTCTTC 1 cut(s) 677
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 2 cut(s) 152, 646
BstDEI CTNAG 3 cut(s) 163, 481, 652
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstMWI GCNNNNNNNGC 5 cut(s) 193, 303, 371, 470, 827
BstNI CCWGG 3 cut(s) 733, 914, 972
BstNSI RCATGY 2 cut(s) 154, 297
BstSCI CCNGG 3 cut(s) 731, 912, 970
BstV1I GCAGC 2 cut(s) 174, 379
BsuRI GGCC 1 cut(s) 505
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 4 cut(s) 239, 757, 792, 797
Cac8I GCNNGC 2 cut(s) 152, 646
CaiI CAGNNNCTG 1 cut(s) 371
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 503, 594, 782
CsiI ACCWGGT 1 cut(s) 912
Csp6I GTAC 3 cut(s) 110, 528, 910
CviAII CATG 5 cut(s) 151, 259, 294, 388, 775
CviQI GTAC 3 cut(s) 110, 528, 910
DdeI CTNAG 3 cut(s) 163, 481, 652
DraI TTTAAA 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 677
EarI CTCTTC 1 cut(s) 677
Eco130I CCWWGG 1 cut(s) 274
Eco47I GGWCC 2 cut(s) 594, 782
Eco57I CTGAAG 2 cut(s) 74, 650
EcoRI GAATTC 1 cut(s) 860
EcoRII CCWGG 3 cut(s) 731, 912, 970
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 5 cut(s) 154, 262, 297, 391, 778
FaqI GGGAC 1 cut(s) 353
FatI CATG 5 cut(s) 150, 258, 293, 387, 774
FauNDI CATATG 2 cut(s) 72, 552
Fnu4HI GCNGC 2 cut(s) 188, 393
Fsp4HI GCNGC 2 cut(s) 188, 393
FspBI CTAG 2 cut(s) 275, 1057
GlaI GCGC 1 cut(s) 186
GluI GCNGC 2 cut(s) 188, 393
GsaI CCCAGC 1 cut(s) 644
GsuI CTGGAG 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 1 cut(s) 505
HhaI GCGC 1 cut(s) 187
Hin1II CATG 5 cut(s) 154, 262, 297, 391, 778
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 894
HindII GTYRAC 1 cut(s) 894
HinfI GANTC 4 cut(s) 67, 199, 722, 839
HphI GGTGA 4 cut(s) 301, 525, 649, 866
Hpy166II GTNNAC 2 cut(s) 169, 894
Hpy188I TCNGA 3 cut(s) 404, 630, 990
Hpy188III TCNNGA 1 cut(s) 259
Hpy8I GTNNAC 2 cut(s) 169, 894
HpyAV CCTTC 1 cut(s) 98
HpyCH4III ACNGT 2 cut(s) 85, 801
HpyCH4V TGCA 9 cut(s) 62, 104, 123, 209, 297, 365, 374, 418, 688
HpyF10VI GCNNNNNNNGC 5 cut(s) 193, 303, 371, 470, 827
HpyF3I CTNAG 3 cut(s) 163, 481, 652
Hsp92II CATG 5 cut(s) 154, 262, 297, 391, 778
HspAI GCGC 1 cut(s) 185
LmnI GCTCC 3 cut(s) 476, 884, 1044
Lsp1109I GCAGC 2 cut(s) 174, 379
MabI ACCWGGT 1 cut(s) 912
MaeI CTAG 2 cut(s) 275, 1057
MaeIII GTNAC 4 cut(s) 178, 289, 602, 655
MboII GAAGA 6 cut(s) 48, 235, 643, 664, 869, 1025
MluCI AATT 3 cut(s) 405, 589, 860
MlyI GAGTC 1 cut(s) 833
MseI TTAA 4 cut(s) 135, 323, 539, 981
MspR9I CCNGG 3 cut(s) 733, 914, 972
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 3 cut(s) 733, 914, 972
MwoI GCNNNNNNNGC 5 cut(s) 193, 303, 371, 470, 827
NdeI CATATG 2 cut(s) 72, 552
NlaIII CATG 5 cut(s) 154, 262, 297, 391, 778
NlaIV GGNNCC 2 cut(s) 478, 504
NmuCI GTSAC 3 cut(s) 178, 289, 655
NspI RCATGY 2 cut(s) 154, 297
PaeI GCATGC 1 cut(s) 154
PagI TCATGA 1 cut(s) 258
PctI GAATGC 1 cut(s) 367
PfeI GAWTC 3 cut(s) 67, 199, 722
PkrI GCNGC 2 cut(s) 189, 394
PleI GAGTC 1 cut(s) 833
PpsI GAGTC 1 cut(s) 833
PshBI ATTAAT 1 cut(s) 981
Psp6I CCWGG 3 cut(s) 731, 912, 970
PspFI CCCAGC 1 cut(s) 640
PspGI CCWGG 3 cut(s) 731, 912, 970
PspN4I GGNNCC 2 cut(s) 478, 504
PspPI GGNCC 3 cut(s) 503, 594, 782
PstNI CAGNNNCTG 1 cut(s) 371
RsaI GTAC 3 cut(s) 111, 529, 911
RsaNI GTAC 3 cut(s) 110, 528, 910
SaqAI TTAA 4 cut(s) 135, 323, 539, 981
SatI GCNGC 2 cut(s) 188, 393
Sau96I GGNCC 3 cut(s) 503, 594, 782
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 1 cut(s) 833
ScrFI CCNGG 3 cut(s) 733, 914, 972
SexAI ACCWGGT 1 cut(s) 912
SinI GGWCC 2 cut(s) 594, 782
SphI GCATGC 1 cut(s) 154
Sse9I AATT 3 cut(s) 405, 589, 860
SspI AATATT 1 cut(s) 247
SspMI CTAG 2 cut(s) 275, 1057
StyD4I CCNGG 3 cut(s) 731, 912, 970
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 2 cut(s) 85, 801
TaqI TCGA 1 cut(s) 350
TasI AATT 3 cut(s) 405, 589, 860
TatI WGTACW 1 cut(s) 527
TfiI GAWTC 3 cut(s) 67, 199, 722
Tru1I TTAA 4 cut(s) 135, 323, 539, 981
Tru9I TTAA 4 cut(s) 135, 323, 539, 981
TscAI CASTG 4 cut(s) 246, 757, 792, 804
TseFI GTSAC 3 cut(s) 178, 289, 655
TseI GCWGC 2 cut(s) 187, 392
Tsp45I GTSAC 3 cut(s) 178, 289, 655
TspDTI ATGAA 3 cut(s) 59, 85, 275
TspRI CASTG 4 cut(s) 246, 757, 792, 804
VpaK11BI GGWCC 2 cut(s) 594, 782
VspI ATTAAT 1 cut(s) 981
XapI RAATTY 1 cut(s) 860
XceI RCATGY 2 cut(s) 154, 297
XcmI CCANNNNNNNNNTGG 1 cut(s) 772
XmaJI CCTAGG 1 cut(s) 274
XspI CTAG 2 cut(s) 275, 1057
ZrmI AGTACT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.