RchiOBHm_Chr2g0100231

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005515
Physical Location & Seq
Reverse (-)
12203430 .. 12210983
7554 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47488

Sequence Viewer

Length: 909 bp
ATGCAGTTTTTTCTCCTGGGACTTATTGGGGTCACCTGCAATCAAGGATTCTACCTCTTGGGTTTGGAGAACACATCAGCCACATTTGTTTCTGCAGTAGAGAATGCAGTCCCTGCAGGAACATTTCTCATGGCTGCCGTATTAAGAATTGAACAAGTTCACTTGAATAGAAAAGATGGCAAAGCCAAAGTGCTTGGAGTTCTGGCTTCTGTTGCAGGAGCCTCAGTCATAACCTTTTACAAGGGGCCAACTATTTATGTTCCTCCAAGGACTTCACCTTCAGGCCAATCCCATTTGCTCCTCTCGTTATTTGGAGATGCCAGGGAGAAGAACTGGACCTTTGGTTGCATTTGTGCTATAGGTCATTGCGTATGTTGGTTTAGCTGGATTGTCCTACAAGGACCTGTTTTGAAGAAGTACCCAGCTCGCCTCTCAGTCACCTCATTTACTCTCTTCTTTGGCATTCTGCAACTTTCAGCAATTGCAGCGTATGTAGAGAGAGACTCTCAAGAGTGGCAGATCCATTCCGGCAGTGCAATCTTGGGCATTCTCTACGTGGGAGTGGTGGCTTCAGCAATGTCTTATGCAATACAGCTATGGGCGATTGACAAGGGAGGACCGGTGTTTGTTTCAGTGTATATGCCTCTACAGACGTTACTCGTAGATATAATGGACTCGGTCATTATGGGTGCACAATTCTACTTTGGAGGCATTGTTGGAGCAGTGCTGATCGTAGCAGGACTATACCTTGTAGTGTGGGGAAAAAGCAAAGAGAGCAAATTTGGCAAAGAAATGAAGTCGAAAACTTCACCAGTGCCATTGAACATTGATAATCAGATTAAATATTATTCTGAAGAAGATAAAGAATCCTCTCTCGTCCAACCATTGCTCCCTATCTCTTCAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

302

Amino Acids

32.81

Weight (kDa)

8.69

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 114 - 252 1.1e-13 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AclWI GGATC 1 cut(s) 514
AcsI RAATTY 1 cut(s) 779
AcuI CTGAAG 3 cut(s) 264, 555, 873
AfaI GTAC 1 cut(s) 419
AgeI ACCGGT 1 cut(s) 619
AgsI TTSAA 5 cut(s) 152, 166, 412, 823, 903
AjnI CCWGG 2 cut(s) 15, 320
AluBI AGCT 3 cut(s) 384, 425, 595
AluI AGCT 3 cut(s) 384, 425, 595
Alw21I GWGCWC 1 cut(s) 694
Alw26I GTCTC 1 cut(s) 495
Alw44I GTGCAC 1 cut(s) 690
AlwI GGATC 1 cut(s) 514
AlwNI CAGNNNCTG 1 cut(s) 113
AoxI GGCC 2 cut(s) 245, 283
ApaLI GTGCAC 1 cut(s) 690
ApeKI GCWGC 2 cut(s) 134, 485
ApoI RAATTY 1 cut(s) 779
AsiGI ACCGGT 1 cut(s) 619
Asp700I GAANNNNTTC 1 cut(s) 156
AspS9I GGNCC 4 cut(s) 245, 336, 401, 617
AsuHPI GGTGA 4 cut(s) 25, 267, 430, 801
AvaII GGWCC 3 cut(s) 336, 401, 617
BaeGI GKGCMC 1 cut(s) 694
Bbv12I GWGCWC 1 cut(s) 694
BbvI GCAGC 2 cut(s) 121, 497
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 2 cut(s) 17, 322
BcoDI GTCTC 1 cut(s) 495
BfmI CTRYAG 4 cut(s) 93, 114, 357, 647
BfuAI ACCTGC 1 cut(s) 44
BisI GCNGC 2 cut(s) 135, 486
BlsI GCNGC 2 cut(s) 136, 487
Bme1390I CCNGG 2 cut(s) 17, 322
Bme18I GGWCC 3 cut(s) 336, 401, 617
BmgT120I GGNCC 4 cut(s) 245, 336, 401, 617
BmiI GGNNCC 2 cut(s) 220, 246
BmrFI CCNGG 2 cut(s) 17, 322
BmsI GCATC 1 cut(s) 307
BplI GAGNNNNNCTC 4 cut(s) 488, 520, 490, 522
BpuEI CTTGAG 1 cut(s) 492
BsaAI YACGTR 1 cut(s) 556
BsaJI CCNNGG 3 cut(s) 16, 266, 321
BsaWI WCCGGW 1 cut(s) 619
BsaXI ACNNNNNCTCC 8 cut(s) 210, 240, 606, 636, 699, 729, 873, 903
Bse118I RCCGGY 1 cut(s) 619
Bse1I ACTGG 2 cut(s) 338, 812
Bse3DI GCAATG 3 cut(s) 364, 582, 884
BseBI CCWGG 2 cut(s) 17, 322
BseDI CCNNGG 3 cut(s) 16, 266, 321
BseMI GCAATG 3 cut(s) 364, 582, 884
BseMII CTCAG 2 cut(s) 237, 447
BseNI ACTGG 2 cut(s) 338, 812
BseRI GAGGAG 1 cut(s) 290
BseSI GKGCMC 1 cut(s) 694
BseXI GCAGC 2 cut(s) 121, 497
BseYI CCCAGC 1 cut(s) 421
BshFI GGCC 2 cut(s) 247, 285
BshTI ACCGGT 1 cut(s) 619
BsiHKAI GWGCWC 1 cut(s) 694
BsiSI CCGG 2 cut(s) 528, 620
BslFI GGGAC 2 cut(s) 33, 95
BsmAI GTCTC 1 cut(s) 495
BsmFI GGGAC 2 cut(s) 33, 95
BsmI GAATGC 3 cut(s) 109, 462, 546
BsnI GGCC 2 cut(s) 247, 285
Bsp1286I GDGCHC 1 cut(s) 694
Bsp143I GATC 2 cut(s) 519, 729
BspANI GGCC 2 cut(s) 247, 285
BspCNI CTCAG 2 cut(s) 236, 446
BspLI GGNNCC 2 cut(s) 220, 246
BspMAI CTGCAG 2 cut(s) 97, 118
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 1 cut(s) 514
BsrDI GCAATG 3 cut(s) 364, 582, 884
BsrFI RCCGGY 1 cut(s) 619
BsrI ACTGG 2 cut(s) 338, 812
BssAI RCCGGY 1 cut(s) 619
BssECI CCNNGG 3 cut(s) 16, 266, 321
BssMI GATC 2 cut(s) 519, 729
BssT1I CCWWGG 1 cut(s) 266
Bst2UI CCWGG 2 cut(s) 17, 322
Bst6I CTCTTC 2 cut(s) 458, 904
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 2 cut(s) 223, 433
BstEII GGTNACC 1 cut(s) 31
BstKTI GATC 2 cut(s) 522, 732
BstMAI GTCTC 1 cut(s) 495
BstMBI GATC 2 cut(s) 519, 729
BstMWI GCNNNNNNNGC 5 cut(s) 113, 212, 485, 774, 783
BstNI CCWGG 2 cut(s) 17, 322
BstPI GGTNACC 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 15, 320
BstSFI CTRYAG 4 cut(s) 93, 114, 357, 647
BstSLI GKGCMC 1 cut(s) 694
BstV1I GCAGC 2 cut(s) 121, 497
BstX2I RGATCY 1 cut(s) 519
BstYI RGATCY 1 cut(s) 519
BsuRI GGCC 2 cut(s) 247, 285
BtsI GCAGTG 2 cut(s) 538, 729
BtsIMutI CAGTG 4 cut(s) 538, 639, 729, 819
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 427
CaiI CAGNNNCTG 1 cut(s) 113
Cfr10I RCCGGY 1 cut(s) 619
Cfr13I GGNCC 4 cut(s) 245, 336, 401, 617
Csp6I GTAC 1 cut(s) 418
CspAI ACCGGT 1 cut(s) 619
CviAII CATG 1 cut(s) 130
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 2 cut(s) 223, 433
DpnI GATC 2 cut(s) 521, 731
DpnII GATC 2 cut(s) 519, 729
Eam1104I CTCTTC 2 cut(s) 458, 904
EarI CTCTTC 2 cut(s) 458, 904
Eco130I CCWWGG 1 cut(s) 266
Eco47I GGWCC 3 cut(s) 336, 401, 617
Eco57I CTGAAG 3 cut(s) 264, 555, 873
Eco91I GGTNACC 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 31
EcoRII CCWGG 2 cut(s) 15, 320
EcoT14I CCWWGG 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 266
FaeI CATG 1 cut(s) 133
FaqI GGGAC 2 cut(s) 33, 95
FatI CATG 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 135, 486
Fsp4HI GCNGC 2 cut(s) 135, 486
GluI GCNGC 2 cut(s) 135, 486
GsaI CCCAGC 1 cut(s) 425
HaeIII GGCC 2 cut(s) 247, 285
HapII CCGG 2 cut(s) 528, 620
Hin1II CATG 1 cut(s) 133
HinfI GANTC 4 cut(s) 48, 503, 674, 866
HpaII CCGG 2 cut(s) 528, 620
HphI GGTGA 4 cut(s) 25, 267, 430, 801
Hpy166II GTNNAC 2 cut(s) 160, 692
Hpy188I TCNGA 2 cut(s) 837, 853
Hpy188III TCNNGA 1 cut(s) 509
Hpy8I GTNNAC 2 cut(s) 160, 692
HpyAV CCTTC 1 cut(s) 288
HpyCH4IV ACGT 2 cut(s) 555, 653
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 212, 485, 774, 783
HpyF3I CTNAG 2 cut(s) 223, 433
HpySE526I ACGT 2 cut(s) 555, 653
Hsp92II CATG 1 cut(s) 133
Kzo9I GATC 2 cut(s) 519, 729
LmnI GCTCC 4 cut(s) 218, 303, 719, 894
Lsp1109I GCAGC 2 cut(s) 121, 497
LweI GCATC 1 cut(s) 307
MaeII ACGT 2 cut(s) 555, 653
MaeIII GTNAC 3 cut(s) 31, 436, 654
MalI GATC 2 cut(s) 521, 731
MboI GATC 2 cut(s) 519, 729
MboII GAAGA 6 cut(s) 340, 424, 445, 866, 869, 891
MfeI CAATTG 1 cut(s) 480
MflI RGATCY 1 cut(s) 519
MhlI GDGCHC 1 cut(s) 694
MluCI AATT 4 cut(s) 147, 480, 695, 779
MlyI GAGTC 2 cut(s) 497, 668
MmeI TCCRAC 2 cut(s) 697, 904
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 2 cut(s) 143, 840
MspI CCGG 2 cut(s) 528, 620
MspR9I CCNGG 2 cut(s) 17, 322
MunI CAATTG 1 cut(s) 480
Mva1269I GAATGC 3 cut(s) 109, 462, 546
MvaI CCWGG 2 cut(s) 17, 322
MwoI GCNNNNNNNGC 5 cut(s) 113, 212, 485, 774, 783
NdeII GATC 2 cut(s) 519, 729
NlaIII CATG 1 cut(s) 133
NlaIV GGNNCC 2 cut(s) 220, 246
NmuCI GTSAC 2 cut(s) 31, 436
PaqCI CACCTGC 1 cut(s) 44
PctI GAATGC 3 cut(s) 109, 462, 546
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 2 cut(s) 48, 866
PflFI GACNNNGTC 1 cut(s) 677
PinAI ACCGGT 1 cut(s) 619
PkrI GCNGC 2 cut(s) 136, 487
PleI GAGTC 2 cut(s) 497, 668
PpsI GAGTC 2 cut(s) 497, 668
Ppu21I YACGTR 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 15, 320
PspEI GGTNACC 1 cut(s) 31
PspFI CCCAGC 1 cut(s) 421
PspGI CCWGG 2 cut(s) 15, 320
PspN4I GGNNCC 2 cut(s) 220, 246
PspPI GGNCC 4 cut(s) 245, 336, 401, 617
PspPPI RGGWCCY 1 cut(s) 401
PstI CTGCAG 2 cut(s) 97, 118
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 519
PsyI GACNNNGTC 1 cut(s) 677
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
SaqAI TTAA 2 cut(s) 143, 840
SatI GCNGC 2 cut(s) 135, 486
Sau3AI GATC 2 cut(s) 519, 729
Sau96I GGNCC 4 cut(s) 245, 336, 401, 617
SbfI CCTGCAGG 1 cut(s) 118
SchI GAGTC 2 cut(s) 497, 668
ScrFI CCNGG 2 cut(s) 17, 322
SdaI CCTGCAGG 1 cut(s) 118
SduI GDGCHC 1 cut(s) 694
SfaNI GCATC 1 cut(s) 307
SfcI CTRYAG 4 cut(s) 93, 114, 357, 647
SinI GGWCC 3 cut(s) 336, 401, 617
SmlI CTYRAG 1 cut(s) 507
SmoI CTYRAG 1 cut(s) 507
Sse8387I CCTGCAGG 1 cut(s) 118
Sse9I AATT 4 cut(s) 147, 480, 695, 779
SspI AATATT 1 cut(s) 845
StyD4I CCNGG 2 cut(s) 15, 320
StyI CCWWGG 1 cut(s) 266
TaiI ACGT 2 cut(s) 558, 656
TaqI TCGA 1 cut(s) 800
TaqII GACCGA 1 cut(s) 667
TasI AATT 4 cut(s) 147, 480, 695, 779
TfiI GAWTC 2 cut(s) 48, 866
Tru1I TTAA 2 cut(s) 143, 840
Tru9I TTAA 2 cut(s) 143, 840
TscAI CASTG 4 cut(s) 538, 639, 729, 819
TseFI GTSAC 2 cut(s) 31, 436
TseI GCWGC 2 cut(s) 134, 485
Tsp45I GTSAC 2 cut(s) 31, 436
TspDTI ATGAA 1 cut(s) 809
TspRI CASTG 4 cut(s) 538, 639, 729, 819
Tth111I GACNNNGTC 1 cut(s) 677
VneI GTGCAC 1 cut(s) 690
VpaK11BI GGWCC 3 cut(s) 336, 401, 617
XapI RAATTY 1 cut(s) 779
XmnI GAANNNNTTC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.