FvH4_1g19780
BHLH Family

Transcription factor bHLH131-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005834
Physical Location & Seq
Forward (+)
11992405 .. 11993217
813 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19780.t1

Sequence Viewer

Length: 645 bp
ATGGGTTCCGCCCCAAGCTATTTTTCCAAGCAAATTGGCTTCCCTCAACGCTATGGTATAGGTCAAGTGAATAGCATCTACAGCAAAAAATTCACTAAATCATTATCAACAGCAGAAGCCAAAGTATTTTCGGCCAAGAAACACAGTGAGTCAGAGAGAAGACGAAGGATGCGGATCAATAGTCAATATGCAGCTCTTCGTAAAATCCTTCCAAACTTGATCAAAATGGACAAGGCTTCTGTGCTCGCTGAGACCGTCCGACAAGTAAGGGAGCTAAAGAAGGTGGTAACAGACATTGACGCAGCATGTCGTGGCAGTGGCAGTGAGTGTGTTATTCCCAGCGGGGTTAACAAGTTGAGTTTAGAGAAGTGTGAAGGCAAGCAAGAAGGTCTTGTGAAGGCAACATTTAGCTGCGAAGATAGGGCAGGGCTGATATCGGACATGACTAGGGAATTGAGGTCAAGGAAAGGGAAGGTGGTGAAGGCGGAGATGGTGACAGTAGGTGGGAGGACTAAGATTGTGCTGTGGGTGAAAGGGTTGGGGGCTGGAAGTGAAGGGATAGTGTCCCTTAAGAAGGCCTTAAATAAGGTGATTGTTGACAGGCCAAGCATTATTCAGAAGCTACATCTCCCTAAGCGACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

215

Amino Acids

23.61

Weight (kDa)

10.23

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 46 - 92 2.3e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 9, 172, 342, 485
AclWI GGATC 1 cut(s) 182
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 89
AfiI CCNNNNNNNGG 1 cut(s) 574
AflII CTTAAG 1 cut(s) 569
AjuI GAANNNNNNNTTGG 2 cut(s) 7, 39
AluBI AGCT 5 cut(s) 18, 194, 274, 411, 622
AluI AGCT 5 cut(s) 18, 194, 274, 411, 622
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 1 cut(s) 182
AoxI GGCC 3 cut(s) 132, 576, 602
ApeKI GCWGC 3 cut(s) 191, 302, 411
ApoI RAATTY 1 cut(s) 89
AsuHPI GGTGA 4 cut(s) 490, 505, 541, 601
BbsI GAAGAC 1 cut(s) 166
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 203, 314, 398
BccI CCATC 1 cut(s) 484
BclI TGATCA 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 1 cut(s) 447
BfmI CTRYAG 1 cut(s) 79
BfrI CTTAAG 1 cut(s) 569
BisI GCNGC 3 cut(s) 192, 303, 412
BlsI GCNGC 3 cut(s) 193, 304, 413
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 2 cut(s) 84, 159
BpiI GAAGAC 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 633
BsaBI GATNNNNATC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 245
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse8I GATNNNNATC 1 cut(s) 173
BseGI GGATG 1 cut(s) 174
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 574
BseMII CTCAG 1 cut(s) 240
BseXI GCAGC 3 cut(s) 203, 314, 398
BseYI CCCAGC 1 cut(s) 338
BshFI GGCC 3 cut(s) 134, 578, 604
BsiHKAI GWGCWC 1 cut(s) 246
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 1 cut(s) 574
BsmAI GTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 3 cut(s) 134, 578, 604
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 2 cut(s) 174, 219
BspACI CCGC 4 cut(s) 9, 172, 342, 485
BspANI GGCC 3 cut(s) 134, 578, 604
BspCNI CTCAG 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 1 cut(s) 182
BspQI GCTCTTC 1 cut(s) 201
BspTI CTTAAG 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 245
BssMI GATC 2 cut(s) 174, 219
Bst4CI ACNGT 3 cut(s) 146, 256, 499
Bst6I CTCTTC 1 cut(s) 201
BstAFI CTTAAG 1 cut(s) 569
BstC8I GCNNGC 2 cut(s) 246, 380
BstDEI CTNAG 3 cut(s) 249, 513, 633
BstENI CCTNNNNNAGG 1 cut(s) 572
BstF5I GGATG 1 cut(s) 174
BstKTI GATC 2 cut(s) 177, 222
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 174, 219
BstMWI GCNNNNNNNGC 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 309
BstSFI CTRYAG 1 cut(s) 79
BstV1I GCAGC 3 cut(s) 203, 314, 398
BstV2I GAAGAC 1 cut(s) 166
BsuRI GGCC 3 cut(s) 134, 578, 604
BtsCI GGATG 1 cut(s) 174
BtsI GCAGTG 2 cut(s) 322, 328
BtsIMutI CAGTG 3 cut(s) 151, 322, 328
Cac8I GCNNGC 2 cut(s) 246, 380
CseI GACGC 1 cut(s) 308
CviAII CATG 2 cut(s) 306, 442
DdeI CTNAG 3 cut(s) 249, 513, 633
DpnI GATC 2 cut(s) 176, 221
DpnII GATC 2 cut(s) 174, 219
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 500
Eco147I AGGCCT 1 cut(s) 578
Eco31I GGTCTC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 435
EcoNI CCTNNNNNAGG 1 cut(s) 572
EcoRV GATATC 1 cut(s) 435
FaeI CATG 2 cut(s) 309, 445
FaiI YATR 6 cut(s) 54, 59, 189, 307, 443, 643
FalI AAGNNNNNCTT 4 cut(s) 375, 407, 563, 595
FaqI GGGAC 1 cut(s) 550
FatI CATG 2 cut(s) 305, 441
FauI CCCGC 1 cut(s) 335
FbaI TGATCA 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 192, 303, 412
FokI GGATG 1 cut(s) 181
Fsp4HI GCNGC 3 cut(s) 192, 303, 412
FspBI CTAG 1 cut(s) 447
GluI GCNGC 3 cut(s) 192, 303, 412
GsaI CCCAGC 1 cut(s) 342
HaeIII GGCC 3 cut(s) 134, 578, 604
HgaI GACGC 1 cut(s) 308
Hin1II CATG 2 cut(s) 309, 445
HincII GTYRAC 2 cut(s) 349, 598
HindII GTYRAC 2 cut(s) 349, 598
HinfI GANTC 1 cut(s) 149
HpaI GTTAAC 1 cut(s) 349
HphI GGTGA 4 cut(s) 490, 505, 541, 601
Hpy166II GTNNAC 2 cut(s) 349, 598
Hpy188I TCNGA 4 cut(s) 154, 260, 439, 618
Hpy8I GTNNAC 2 cut(s) 349, 598
HpyCH4III ACNGT 3 cut(s) 146, 256, 499
HpyCH4V TGCA 1 cut(s) 191
HpyF10VI GCNNNNNNNGC 1 cut(s) 81
HpyF3I CTNAG 3 cut(s) 249, 513, 633
Hsp92II CATG 2 cut(s) 309, 445
Ksp22I TGATCA 1 cut(s) 219
KspAI GTTAAC 1 cut(s) 349
Kzo9I GATC 2 cut(s) 174, 219
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 4 cut(s) 352, 411, 531, 586
Lsp1109I GCAGC 3 cut(s) 203, 314, 398
LweI GCATC 2 cut(s) 84, 159
MaeI CTAG 1 cut(s) 447
MaeIII GTNAC 2 cut(s) 286, 493
MalI GATC 2 cut(s) 176, 221
MboI GATC 2 cut(s) 174, 219
MboII GAAGA 3 cut(s) 171, 188, 428
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 3 cut(s) 33, 89, 452
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 3 cut(s) 54, 450, 501
MseI TTAA 3 cut(s) 348, 570, 581
MspA1I CMGCKG 1 cut(s) 342
MspCI CTTAAG 1 cut(s) 569
MwoI GCNNNNNNNGC 1 cut(s) 81
NdeII GATC 2 cut(s) 174, 219
NlaIII CATG 2 cut(s) 309, 445
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 1 cut(s) 493
NspI RCATGY 1 cut(s) 309
PceI AGGCCT 1 cut(s) 578
PciSI GCTCTTC 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PkrI GCNGC 3 cut(s) 193, 304, 413
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 338
PspN4I GGNNCC 1 cut(s) 7
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 3 cut(s) 348, 570, 581
SatI GCNGC 3 cut(s) 192, 303, 412
Sau3AI GATC 2 cut(s) 174, 219
SchI GAGTC 1 cut(s) 158
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 2 cut(s) 84, 159
SfcI CTRYAG 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 3 cut(s) 33, 89, 452
SseBI AGGCCT 1 cut(s) 578
SsiI CCGC 4 cut(s) 9, 172, 342, 485
SspMI CTAG 1 cut(s) 447
StuI AGGCCT 1 cut(s) 578
TaaI ACNGT 3 cut(s) 146, 256, 499
TasI AATT 3 cut(s) 33, 89, 452
Tru1I TTAA 3 cut(s) 348, 570, 581
Tru9I TTAA 3 cut(s) 348, 570, 581
TscAI CASTG 3 cut(s) 151, 322, 328
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 3 cut(s) 191, 302, 411
Tsp45I GTSAC 1 cut(s) 493
TspRI CASTG 3 cut(s) 151, 322, 328
Vha464I CTTAAG 1 cut(s) 569
XagI CCTNNNNNAGG 1 cut(s) 572
XapI RAATTY 1 cut(s) 89
XceI RCATGY 1 cut(s) 309
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.