MD15G1314000.v1.1
BHLH Family

Transcription factor bHLH131-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005834
Physical Location & Seq
Forward (+)
31763197 .. 31763931
735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1314000.v1.1.491

Sequence Viewer

Length: 735 bp
ATGCAATCCACTCAAAGTTATTATCCTAAGCAAATTAGCTTCCCCAAAGGCCAACGAGTACTCCTAACGAATAATATCTACAACAAAAACTTCTTCAAACCAAAATCAAAGGCAGAAGCCAAAGTAATTGCCGCCAAGAAACACAGTGAGTCAGAGAAAAGGCGAAGGATGAGAATCAACAGTCAATATACCACTCTTCGTGCTATCCTCCCAGACTTAATCAAAGTATATTATTTAATTGATTTGATGGAATTTTGTTTGAATGTGTATGGTGTATTATGGAAAATGTCTCGCAAACTTTCCTCACTTAGAATACTTGACATACAGATGGACAAGGCATCTGTGCTTGGAGAGACAGTCAGGAAAGTAAGGGAGCTTAAAAAGGCGTTAACAGAAGTTGAAGCAGCTTGTCATTGCGGGGACAGGAGGGATGAGTGCGTTTTACCTGGTGGGGTTGACAAGTTAAGTGTCGAACAATGCGAGGGGGAGAAAGGAGGGCTTGTGAAGGCCACATTCAGCTGTGAGGATAGGCTGGGGCTCATTTCGGACATGACAAGGGCACTGAGTTGTGTAAAGGGAAGGGTTGTTAGGGCTGAGATGGTGACAGTCGGTGGAAGGAGTAAAAATGCATTGTGGGTGCAAGGGTTAGGTGGTGGAAATGAAGGGGTGGTGGCGCTGAAGAGAGCACTGAAGGTGGTTATTGATCGCCCTGTTTTGCCAAGGAATATTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

245

Amino Acids

27.39

Weight (kDa)

9.79

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 46 - 107 2.2e-07 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 132, 417
AcsI RAATTY 1 cut(s) 251
AcuI CTGAAG 2 cut(s) 698, 710
AfaI GTAC 1 cut(s) 60
AgsI TTSAA 3 cut(s) 97, 262, 401
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 4 cut(s) 39, 376, 407, 519
AluI AGCT 4 cut(s) 39, 376, 407, 519
Alw21I GWGCWC 1 cut(s) 688
Alw26I GTCTC 2 cut(s) 294, 347
AoxI GGCC 2 cut(s) 49, 507
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 1 cut(s) 251
AspLEI GCGC 1 cut(s) 676
AsuHPI GGTGA 1 cut(s) 613
BaeGI GKGCMC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 688
BbvI GCAGC 1 cut(s) 416
BccI CCATC 3 cut(s) 241, 322, 592
BciT130I CCWGG 1 cut(s) 447
BcoDI GTCTC 2 cut(s) 294, 347
BfoI RGCGCY 1 cut(s) 677
BisI GCNGC 2 cut(s) 132, 405
BlsI GCNGC 2 cut(s) 133, 406
BmcAI AGTACT 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 447
BmrFI CCNGG 1 cut(s) 447
BmsI GCATC 1 cut(s) 347
Bpu10I CCTNAGC 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 719
BsaXI ACNNNNNCTCC 6 cut(s) 45, 75, 342, 372, 486, 516
Bse3DI GCAATG 1 cut(s) 412
Bse8I GATNNNNATC 1 cut(s) 173
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 1 cut(s) 719
BseGI GGATG 2 cut(s) 174, 436
BseJI GATNNNNATC 1 cut(s) 173
BseMI GCAATG 1 cut(s) 412
BseMII CTCAG 2 cut(s) 554, 585
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 1 cut(s) 416
BseYI CCCAGC 1 cut(s) 532
BshFI GGCC 2 cut(s) 51, 509
BsiHKAI GWGCWC 1 cut(s) 688
BslFI GGGAC 1 cut(s) 434
BsmAI GTCTC 2 cut(s) 294, 347
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 2 cut(s) 51, 509
Bsp1286I GDGCHC 3 cut(s) 540, 562, 688
Bsp143I GATC 1 cut(s) 703
BspACI CCGC 2 cut(s) 132, 417
BspANI GGCC 2 cut(s) 51, 509
BspCNI CTCAG 2 cut(s) 555, 586
BsrDI GCAATG 1 cut(s) 412
BssECI CCNNGG 1 cut(s) 719
BssMI GATC 1 cut(s) 703
BssT1I CCWWGG 1 cut(s) 719
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 4 cut(s) 146, 182, 358, 607
Bst6I CTCTTC 2 cut(s) 201, 674
BstDEI CTNAG 4 cut(s) 27, 308, 563, 594
BstF5I GGATG 2 cut(s) 174, 436
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 1 cut(s) 706
BstMAI GTCTC 2 cut(s) 294, 347
BstMBI GATC 1 cut(s) 703
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 1 cut(s) 445
BstSLI GKGCMC 1 cut(s) 562
BstV1I GCAGC 1 cut(s) 416
BsuRI GGCC 2 cut(s) 51, 509
BtsCI GGATG 2 cut(s) 174, 436
BtsIMutI CAGTG 3 cut(s) 151, 560, 686
CfoI GCGC 1 cut(s) 676
CsiI ACCWGGT 1 cut(s) 445
Csp6I GTAC 1 cut(s) 59
CviAII CATG 1 cut(s) 550
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 4 cut(s) 27, 308, 563, 594
DpnI GATC 1 cut(s) 705
DpnII GATC 1 cut(s) 703
Eam1104I CTCTTC 2 cut(s) 201, 674
EarI CTCTTC 2 cut(s) 201, 674
Eco130I CCWWGG 1 cut(s) 719
Eco24I GRGCYC 1 cut(s) 540
Eco57I CTGAAG 2 cut(s) 698, 710
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 719
EcoT22I ATGCAT 1 cut(s) 631
EcoT38I GRGCYC 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 719
FaeI CATG 1 cut(s) 553
FaiI YATR 6 cut(s) 189, 229, 270, 280, 323, 551
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 1 cut(s) 434
FatI CATG 1 cut(s) 549
FauI CCCGC 1 cut(s) 410
Fnu4HI GCNGC 2 cut(s) 132, 405
FokI GGATG 2 cut(s) 181, 443
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 2 cut(s) 132, 405
GlaI GCGC 1 cut(s) 675
GluI GCNGC 2 cut(s) 132, 405
GsaI CCCAGC 1 cut(s) 536
HaeII RGCGCY 1 cut(s) 677
HaeIII GGCC 2 cut(s) 51, 509
HhaI GCGC 1 cut(s) 676
Hin1II CATG 1 cut(s) 553
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 2 cut(s) 390, 457
HindII GTYRAC 2 cut(s) 390, 457
HinfI GANTC 2 cut(s) 149, 174
HpaI GTTAAC 1 cut(s) 390
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 2 cut(s) 390, 457
Hpy188I TCNGA 3 cut(s) 154, 547, 734
Hpy188III TCNNGA 1 cut(s) 361
Hpy8I GTNNAC 2 cut(s) 390, 457
HpyAV CCTTC 6 cut(s) 159, 499, 573, 609, 656, 685
HpyCH4III ACNGT 4 cut(s) 146, 182, 358, 607
HpyCH4V TGCA 3 cut(s) 4, 629, 640
HpyF3I CTNAG 4 cut(s) 27, 308, 563, 594
Hsp92II CATG 1 cut(s) 553
HspAI GCGC 1 cut(s) 674
KspAI GTTAAC 1 cut(s) 390
Kzo9I GATC 1 cut(s) 703
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 7 cut(s) 225, 346, 409, 432, 459, 518, 723
Lsp1109I GCAGC 1 cut(s) 416
LweI GCATC 1 cut(s) 347
MabI ACCWGGT 1 cut(s) 445
MaeIII GTNAC 1 cut(s) 601
MalI GATC 1 cut(s) 705
MboI GATC 1 cut(s) 703
MboII GAAGA 3 cut(s) 85, 188, 691
MhlI GDGCHC 3 cut(s) 540, 562, 688
MluCI AATT 4 cut(s) 33, 126, 237, 251
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 6 cut(s) 218, 313, 420, 475, 488, 517
Mph1103I ATGCAT 1 cut(s) 631
MseI TTAA 5 cut(s) 218, 236, 378, 389, 464
MslI CAYNNNNRTG 1 cut(s) 326
MspA1I CMGCKG 1 cut(s) 519
MspR9I CCNGG 1 cut(s) 447
MvaI CCWGG 1 cut(s) 447
NdeII GATC 1 cut(s) 703
NlaIII CATG 1 cut(s) 553
NmuCI GTSAC 1 cut(s) 601
NsiI ATGCAT 1 cut(s) 631
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PfeI GAWTC 1 cut(s) 174
PkrI GCNGC 2 cut(s) 133, 406
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 445
PspFI CCCAGC 1 cut(s) 532
PspGI CCWGG 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 519
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
RseI CAYNNNNRTG 1 cut(s) 326
SaqAI TTAA 5 cut(s) 218, 236, 378, 389, 464
SatI GCNGC 2 cut(s) 132, 405
Sau3AI GATC 1 cut(s) 703
ScaI AGTACT 1 cut(s) 60
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 447
SduI GDGCHC 3 cut(s) 540, 562, 688
SetI ASST 7 cut(s) 41, 378, 409, 448, 521, 652, 696
SexAI ACCWGGT 1 cut(s) 445
SfaNI GCATC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 4 cut(s) 33, 126, 237, 251
SsiI CCGC 2 cut(s) 132, 417
SspI AATATT 1 cut(s) 727
StyD4I CCNGG 1 cut(s) 445
StyI CCWWGG 1 cut(s) 719
TaaI ACNGT 4 cut(s) 146, 182, 358, 607
TaqI TCGA 1 cut(s) 471
TasI AATT 4 cut(s) 33, 126, 237, 251
TatI WGTACW 1 cut(s) 58
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 174
Tru1I TTAA 5 cut(s) 218, 236, 378, 389, 464
Tru9I TTAA 5 cut(s) 218, 236, 378, 389, 464
TscAI CASTG 3 cut(s) 151, 567, 693
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 1 cut(s) 675
TspRI CASTG 3 cut(s) 151, 567, 693
XapI RAATTY 1 cut(s) 251
ZrmI AGTACT 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.