MD15G1384300.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005834
Physical Location & Seq
Reverse (-)
47416469 .. 47417727
1259 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1384300.v1.1.491

Sequence Viewer

Length: 705 bp
ATGCTTCCTTTCCAGAACTACTCTGAGTCGGAGCGACAGAGCTGGTCCAGTCAAAACCGCAACAACAACAACCTAAGGGACGGCGCAGATTCCATCCTGAGCTCGGCGGCATCGGCGACGGAGACCGCCAAGTCAGCAGAAGCATCCACCAGCCACAAGGAAGCCGAGAGGCGGCGCAGGCAGCGTATCAACGCCCACCTCTCCACCCTCCGTACTCTCCTCCCCAACACCACCCGGACGGACAAGGCGTCGTTGCTGGCGGAGGTGGTGCAGCACGTGAGGGAGCTGAGGAGGCAAGCTGATGACCTGGCGCGACAAGGCCGGGCCGGGTCGGAGGCCTGGCCGTTTCCGGCCGAGTCGGACGAGGTGACGCTGAACTACTGCAACGGCGGGACCAAGCTGTTGAAGGCGACACTGAGCTGCGAAGACAGACCGGGTCTAAACCGGGACTTGGTACAGGCAATAAGGTCTGTTCGGGCAAGGGCTGTGCGGGCTGAGATGATGACGGTCGGCAGTCGGACCAAGAATGTGGTGGTGATGCAGTGGGCTGGCGGTGGCGGGGAGGAGGGGTTTAGGGCTTTGAAGCGGGCATTGAAGGCTGTGGTGGAGAATCGGACCTCCGGTTCTAGGCTGGGTAAGTTGGTTTCGAGTAATAACCGGGGTCGGGTTTTTGAGGATGCTGACGAGGATATGAGTTTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

235

Amino Acids

25.95

Weight (kDa)

9.86

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 50 - 97 3.7e-12 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 130
AccII CGCG 1 cut(s) 313
AcoI YGGCCR 2 cut(s) 341, 351
AcvI CACGTG 1 cut(s) 277
AcyI GRCGYC 1 cut(s) 248
AfaI GTAC 2 cut(s) 214, 456
AfiI CCNNNNNNNGG 5 cut(s) 103, 171, 451, 627, 664
AgsI TTSAA 3 cut(s) 406, 583, 595
AhdI GACNNNNNGTC 1 cut(s) 247
AjnI CCWGG 2 cut(s) 306, 338
AloI GAACNNNNNNTCC 2 cut(s) 607, 639
AluBI AGCT 6 cut(s) 42, 102, 286, 299, 400, 420
AluI AGCT 6 cut(s) 42, 102, 286, 299, 400, 420
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 116
AoxI GGCC 5 cut(s) 319, 324, 336, 341, 351
ApeKI GCWGC 3 cut(s) 181, 271, 420
AspLEI GCGC 3 cut(s) 86, 177, 313
AspS9I GGNCC 5 cut(s) 45, 324, 393, 519, 615
AsuC2I CCSGG 6 cut(s) 235, 323, 328, 435, 446, 659
AsuHPI GGTGA 2 cut(s) 379, 547
AvaII GGWCC 4 cut(s) 45, 393, 519, 615
AxyI CCTNAGG 1 cut(s) 74
BanII GRGCYC 1 cut(s) 104
BbrPI CACGTG 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 104
BbvCI CCTCAGC 1 cut(s) 287
BbvI GCAGC 3 cut(s) 193, 283, 407
BccI CCATC 1 cut(s) 101
BceAI ACGGC 3 cut(s) 97, 328, 403
BciT130I CCWGG 2 cut(s) 308, 340
BcnI CCSGG 6 cut(s) 235, 323, 328, 435, 446, 659
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 1 cut(s) 627
BisI GCNGC 5 cut(s) 108, 173, 182, 272, 421
BlsI GCNGC 5 cut(s) 109, 174, 183, 273, 422
Bme1390I CCNGG 8 cut(s) 235, 308, 323, 328, 340, 435, 446, 659
Bme18I GGWCC 4 cut(s) 45, 393, 519, 615
BmeRI GACNNNNNGTC 1 cut(s) 247
BmgT120I GGNCC 5 cut(s) 45, 324, 393, 519, 615
BmiI GGNNCC 1 cut(s) 394
BmrFI CCNGG 8 cut(s) 235, 308, 323, 328, 340, 435, 446, 659
BmsI GCATC 4 cut(s) 119, 152, 528, 667
BpiI GAAGAC 1 cut(s) 432
Bpu10I CCTNAGC 2 cut(s) 98, 287
BpuMI CCSGG 6 cut(s) 235, 323, 328, 435, 446, 659
BsaAI YACGTR 1 cut(s) 277
BsaHI GRCGYC 1 cut(s) 248
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 658
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 554, 584
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 171, 451, 627, 664
Bse1I ACTGG 1 cut(s) 48
Bse21I CCTNAGG 1 cut(s) 74
BseBI CCWGG 2 cut(s) 308, 340
BseDI CCNNGG 1 cut(s) 658
BseGI GGATG 3 cut(s) 93, 143, 682
BseLI CCNNNNNNNGG 5 cut(s) 103, 171, 451, 627, 664
BseMII CTCAG 5 cut(s) 15, 89, 278, 407, 486
BseNI ACTGG 1 cut(s) 48
BseRI GAGGAG 3 cut(s) 209, 304, 578
BseX3I CGGCCG 1 cut(s) 351
BseXI GCAGC 3 cut(s) 193, 283, 407
BseYI CCCAGC 1 cut(s) 631
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 1 cut(s) 313
Bsh1285I CGRYCG 2 cut(s) 354, 510
BshFI GGCC 5 cut(s) 321, 326, 338, 343, 353
BsiEI CGRYCG 2 cut(s) 354, 510
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 8 cut(s) 235, 322, 327, 350, 434, 445, 621, 658
BslFI GGGAC 3 cut(s) 92, 406, 461
BslI CCNNNNNNNGG 5 cut(s) 103, 171, 451, 627, 664
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 3 cut(s) 92, 406, 461
BsnI GGCC 5 cut(s) 321, 326, 338, 343, 353
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 104
BspANI GGCC 5 cut(s) 321, 326, 338, 343, 353
BspCNI CTCAG 5 cut(s) 16, 90, 279, 408, 487
BspFNI CGCG 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 116
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 658
BssNI GRCGYC 1 cut(s) 248
Bst2UI CCWGG 2 cut(s) 308, 340
Bst4CI ACNGT 1 cut(s) 508
BstACI GRCGYC 1 cut(s) 248
BstBAI YACGTR 1 cut(s) 277
BstC8I GCNNGC 6 cut(s) 179, 258, 297, 492, 550, 588
BstDEI CTNAG 6 cut(s) 24, 74, 98, 287, 416, 495
BstF5I GGATG 3 cut(s) 93, 143, 682
BstFNI CGCG 1 cut(s) 313
BstHHI GCGC 3 cut(s) 86, 177, 313
BstMAI GTCTC 1 cut(s) 116
BstMCI CGRYCG 2 cut(s) 354, 510
BstMWI GCNNNNNNNGC 7 cut(s) 113, 134, 178, 181, 292, 491, 596
BstNI CCWGG 2 cut(s) 308, 340
BstSCI CCNGG 8 cut(s) 233, 306, 321, 326, 338, 433, 444, 657
BstUI CGCG 1 cut(s) 313
BstV1I GCAGC 3 cut(s) 193, 283, 407
BstV2I GAAGAC 1 cut(s) 432
BstXI CCANNNNNNTGG 1 cut(s) 529
BstZI CGGCCG 1 cut(s) 351
Bsu36I CCTNAGG 1 cut(s) 74
BsuRI GGCC 5 cut(s) 321, 326, 338, 343, 353
BtsCI GGATG 3 cut(s) 93, 143, 682
BtsI GCAGTG 1 cut(s) 548
BtsIMutI CAGTG 2 cut(s) 413, 548
Cac8I GCNNGC 6 cut(s) 179, 258, 297, 492, 550, 588
CfoI GCGC 3 cut(s) 86, 177, 313
Cfr13I GGNCC 5 cut(s) 45, 324, 393, 519, 615
CseI GACGC 2 cut(s) 237, 379
Csp6I GTAC 2 cut(s) 213, 455
CviQI GTAC 2 cut(s) 213, 455
DdeI CTNAG 6 cut(s) 24, 74, 98, 287, 416, 495
DrdI GACNNNNNNGTC 1 cut(s) 130
DriI GACNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 130
EaeI YGGCCR 2 cut(s) 341, 351
EagI CGGCCG 1 cut(s) 351
Eam1105I GACNNNNNGTC 1 cut(s) 247
EciI GGCGGA 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 102
EclXI CGGCCG 1 cut(s) 351
Eco147I AGGCCT 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 104
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 4 cut(s) 45, 393, 519, 615
Eco52I CGGCCG 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 102
Eco72I CACGTG 1 cut(s) 277
Eco81I CCTNAGG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 102
EcoRII CCWGG 2 cut(s) 306, 338
EcoT38I GRGCYC 1 cut(s) 104
FaiI YATR 1 cut(s) 692
FaqI GGGAC 3 cut(s) 92, 406, 461
FauI CCCGC 4 cut(s) 383, 483, 551, 579
Fnu4HI GCNGC 5 cut(s) 108, 173, 182, 272, 421
FokI GGATG 3 cut(s) 80, 130, 689
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 5 cut(s) 108, 173, 182, 272, 421
FspBI CTAG 1 cut(s) 627
GlaI GCGC 3 cut(s) 85, 176, 312
GluI GCNGC 5 cut(s) 108, 173, 182, 272, 421
GsaI CCCAGC 1 cut(s) 635
HaeIII GGCC 5 cut(s) 321, 326, 338, 343, 353
HapII CCGG 8 cut(s) 235, 322, 327, 350, 434, 445, 621, 658
HgaI GACGC 2 cut(s) 237, 379
HhaI GCGC 3 cut(s) 86, 177, 313
Hin1I GRCGYC 1 cut(s) 248
Hin6I GCGC 3 cut(s) 84, 175, 311
HinP1I GCGC 3 cut(s) 84, 175, 311
HinfI GANTC 4 cut(s) 26, 89, 356, 610
HpaII CCGG 8 cut(s) 235, 322, 327, 350, 434, 445, 621, 658
HphI GGTGA 2 cut(s) 379, 547
Hpy188I TCNGA 6 cut(s) 25, 31, 334, 361, 519, 615
Hpy188III TCNNGA 2 cut(s) 13, 97
Hpy99I CGWCG 2 cut(s) 121, 253
HpyAV CCTTC 2 cut(s) 400, 589
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 271, 384, 541
HpyF10VI GCNNNNNNNGC 7 cut(s) 113, 134, 178, 181, 292, 491, 596
HpyF3I CTNAG 6 cut(s) 24, 74, 98, 287, 416, 495
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 248
HspAI GCGC 3 cut(s) 84, 175, 311
LmnI GCTCC 2 cut(s) 31, 283
Lsp1109I GCAGC 3 cut(s) 193, 283, 407
LweI GCATC 4 cut(s) 119, 152, 528, 667
MaeI CTAG 1 cut(s) 627
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 367
MboII GAAGA 1 cut(s) 437
MhlI GDGCHC 1 cut(s) 104
MlyI GAGTC 2 cut(s) 35, 365
MmeI TCCRAC 4 cut(s) 9, 312, 339, 497
MspI CCGG 8 cut(s) 235, 322, 327, 350, 434, 445, 621, 658
MspR9I CCNGG 8 cut(s) 235, 308, 323, 328, 340, 435, 446, 659
MvaI CCWGG 2 cut(s) 308, 340
MvnI CGCG 1 cut(s) 313
MwoI GCNNNNNNNGC 7 cut(s) 113, 134, 178, 181, 292, 491, 596
NciI CCSGG 6 cut(s) 235, 323, 328, 435, 446, 659
NlaIV GGNNCC 1 cut(s) 394
NmeAIII GCCGAG 3 cut(s) 83, 190, 379
NmuCI GTSAC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 338
PcsI WCGNNNNNNNCGW 1 cut(s) 245
PfeI GAWTC 2 cut(s) 89, 610
PflFI GACNNNGTC 1 cut(s) 435
PkrI GCNGC 5 cut(s) 109, 174, 183, 273, 422
PleI GAGTC 2 cut(s) 34, 364
PmaCI CACGTG 1 cut(s) 277
PmlI CACGTG 1 cut(s) 277
PpsI GAGTC 2 cut(s) 34, 364
Ppu21I YACGTR 1 cut(s) 277
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 306, 338
PspCI CACGTG 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 631
PspGI CCWGG 2 cut(s) 306, 338
PspN4I GGNNCC 1 cut(s) 394
PspPI GGNCC 5 cut(s) 45, 324, 393, 519, 615
PsyI GACNNNGTC 1 cut(s) 435
RsaI GTAC 2 cut(s) 214, 456
RsaNI GTAC 2 cut(s) 213, 455
SacI GAGCTC 1 cut(s) 104
SatI GCNGC 5 cut(s) 108, 173, 182, 272, 421
Sau96I GGNCC 5 cut(s) 45, 324, 393, 519, 615
SchI GAGTC 2 cut(s) 35, 365
ScrFI CCNGG 8 cut(s) 235, 308, 323, 328, 340, 435, 446, 659
SduI GDGCHC 1 cut(s) 104
SfaNI GCATC 4 cut(s) 119, 152, 528, 667
SinI GGWCC 4 cut(s) 45, 393, 519, 615
SseBI AGGCCT 1 cut(s) 338
SspMI CTAG 1 cut(s) 627
SstI GAGCTC 1 cut(s) 104
StuI AGGCCT 1 cut(s) 338
StyD4I CCNGG 8 cut(s) 233, 306, 321, 326, 338, 433, 444, 657
TaaI ACNGT 1 cut(s) 508
TaiI ACGT 1 cut(s) 279
TaqI TCGA 1 cut(s) 647
TauI GCSGC 2 cut(s) 110, 175
TfiI GAWTC 2 cut(s) 89, 610
TscAI CASTG 2 cut(s) 420, 548
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 3 cut(s) 181, 271, 420
Tsp45I GTSAC 1 cut(s) 367
TspGWI ACGGA 3 cut(s) 134, 200, 254
TspRI CASTG 2 cut(s) 420, 548
Tth111I GACNNNGTC 1 cut(s) 435
VpaK11BI GGWCC 4 cut(s) 45, 393, 519, 615
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XspI CTAG 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.