RchiOBHm_Chr2g0111351
BHLH Family

Transcription factor bHLH131-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005834
Physical Location & Seq
Forward (+)
22988391 .. 22989233
843 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48494

Sequence Viewer

Length: 624 bp
ATGCAGATGCGTTCCGCCCCAAGCTATTTTTCCAAGCAAATTGGTTTTCATCAACGCTATCGTATAGATCCAGTGAATAGCATCTACAGAAAAAACTTCTGTAGACCAAAATCAACAGCAGAAGCCAAAGTACTTGCGGCCAAGAAACACAGTGAGTCAGAGAGAAGACGAAGGATGCGAATCAATAGTCAATATACAGCTCTTCGTAAAATCCTCCCAAACTTGATCAAAATGGACAAGGCTTCTGTGCTCGCAGAGACCGTCCGACAAGTAAGGGAGCTTAAGAAGGTGGTAGCAGATACTGAAGCAGCATGTCGTGGCAGTGGCAGTGAGTGTGTTATTCCCAGCGGCGTTAACAAGTTGAGTTTGGAGAAGTGTGAAGGCAAGCAAGAAGGGCTTGTGAAAGCAACATTTAGCTGCGAGGATAGGCCAGGGCTAATATTGGACATGATTAGGGAGCTGAGGTCAGGGAAAGGGAGGGTGGTGAGGGCGGAGATGGTGACAGTAGGTGGGAGGACTAAGAGTGTGTTGTGGGTGAAAGGGTTGGGTGCTGGAAGTGAAGGGATAGTGTGCCTTAAGAAGGCATTAAATAAGGTGATTGTTGACAGGCCAAGCATTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

207

Amino Acids

23.09

Weight (kDa)

10.18

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 48 - 94 3.2e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 103
AciI CCGC 4 cut(s) 15, 137, 348, 491
AclWI GGATC 1 cut(s) 62
AcoI YGGCCR 1 cut(s) 138
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 580
AflII CTTAAG 2 cut(s) 281, 575
AjnI CCWGG 1 cut(s) 430
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 5 cut(s) 24, 200, 280, 417, 460
AluI AGCT 5 cut(s) 24, 200, 280, 417, 460
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 1 cut(s) 302
AoxI GGCC 3 cut(s) 138, 428, 608
ApeKI GCWGC 2 cut(s) 308, 417
ArsI GACNNNNNNTTYG 2 cut(s) 172, 204
AsuHPI GGTGA 4 cut(s) 496, 511, 547, 607
BarI GAAGNNNNNNTAC 2 cut(s) 114, 146
BbsI GAAGAC 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 252
BbvCI CCTCAGC 1 cut(s) 461
BbvI GCAGC 2 cut(s) 320, 404
BccI CCATC 1 cut(s) 490
BciT130I CCWGG 1 cut(s) 432
BclI TGATCA 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 2 cut(s) 85, 100
BfrI CTTAAG 2 cut(s) 281, 575
BisI GCNGC 4 cut(s) 138, 309, 349, 418
BlsI GCNGC 4 cut(s) 139, 310, 350, 419
BmcAI AGTACT 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 432
BmrFI CCNGG 1 cut(s) 432
BmsI GCATC 2 cut(s) 90, 165
BpiI GAAGAC 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 461
BsaBI GATNNNNATC 1 cut(s) 179
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 431
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bsc4I CCNNNNNNNGG 1 cut(s) 580
Bse1I ACTGG 1 cut(s) 71
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 431
BseGI GGATG 1 cut(s) 180
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 580
BseMII CTCAG 1 cut(s) 452
BseNI ACTGG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 320, 404
BseYI CCCAGC 1 cut(s) 344
BshFI GGCC 3 cut(s) 140, 430, 610
BsiHKAI GWGCWC 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 580
BsmAI GTCTC 1 cut(s) 251
BsnI GGCC 3 cut(s) 140, 430, 610
Bso31I GGTCTC 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 2 cut(s) 67, 225
BspACI CCGC 4 cut(s) 15, 137, 348, 491
BspANI GGCC 3 cut(s) 140, 430, 610
BspCNI CTCAG 1 cut(s) 453
BspPI GGATC 1 cut(s) 62
BspQI GCTCTTC 1 cut(s) 207
BspTI CTTAAG 2 cut(s) 281, 575
BspTNI GGTCTC 1 cut(s) 251
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 431
BssMI GATC 2 cut(s) 67, 225
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 3 cut(s) 152, 262, 505
Bst6I CTCTTC 1 cut(s) 207
BstAFI CTTAAG 2 cut(s) 281, 575
BstC8I GCNNGC 2 cut(s) 252, 386
BstDEI CTNAG 2 cut(s) 461, 519
BstENI CCTNNNNNAGG 1 cut(s) 578
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 2 cut(s) 70, 228
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 2 cut(s) 67, 225
BstMWI GCNNNNNNNGC 1 cut(s) 394
BstNI CCWGG 1 cut(s) 432
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 430
BstSFI CTRYAG 2 cut(s) 85, 100
BstV1I GCAGC 2 cut(s) 320, 404
BstV2I GAAGAC 1 cut(s) 172
BstX2I RGATCY 1 cut(s) 67
BstYI RGATCY 1 cut(s) 67
BsuRI GGCC 3 cut(s) 140, 430, 610
BtsCI GGATG 1 cut(s) 180
BtsI GCAGTG 2 cut(s) 328, 334
BtsIMutI CAGTG 4 cut(s) 78, 157, 328, 334
Cac8I GCNNGC 2 cut(s) 252, 386
CaiI CAGNNNCTG 1 cut(s) 302
Csp6I GTAC 1 cut(s) 131
CviAII CATG 2 cut(s) 312, 448
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 2 cut(s) 461, 519
DpnI GATC 2 cut(s) 69, 227
DpnII GATC 2 cut(s) 67, 225
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
EciI GGCGGA 2 cut(s) 4, 506
Eco31I GGTCTC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 324
EcoNI CCTNNNNNAGG 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 430
FaeI CATG 2 cut(s) 315, 451
FaiI YATR 4 cut(s) 65, 195, 313, 449
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 2 cut(s) 311, 447
FbaI TGATCA 1 cut(s) 225
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 4 cut(s) 138, 309, 349, 418
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 4 cut(s) 138, 309, 349, 418
GluI GCNGC 4 cut(s) 138, 309, 349, 418
GsaI CCCAGC 1 cut(s) 348
HaeIII GGCC 3 cut(s) 140, 430, 610
Hin1II CATG 2 cut(s) 315, 451
HincII GTYRAC 2 cut(s) 355, 604
HindII GTYRAC 2 cut(s) 355, 604
HinfI GANTC 2 cut(s) 155, 180
HpaI GTTAAC 1 cut(s) 355
HphI GGTGA 4 cut(s) 496, 511, 547, 607
Hpy166II GTNNAC 3 cut(s) 104, 355, 604
Hpy188I TCNGA 2 cut(s) 160, 266
Hpy8I GTNNAC 3 cut(s) 104, 355, 604
HpyAV CCTTC 6 cut(s) 165, 280, 374, 386, 554, 574
HpyCH4III ACNGT 3 cut(s) 152, 262, 505
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 394
HpyF3I CTNAG 2 cut(s) 461, 519
Hsp92II CATG 2 cut(s) 315, 451
Ksp22I TGATCA 1 cut(s) 225
KspAI GTTAAC 1 cut(s) 355
Kzo9I GATC 2 cut(s) 67, 225
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 2 cut(s) 277, 457
LpnPI CCDG 7 cut(s) 84, 358, 417, 444, 453, 537, 592
Lsp1109I GCAGC 2 cut(s) 320, 404
LweI GCATC 2 cut(s) 90, 165
MaeIII GTNAC 1 cut(s) 499
MalI GATC 2 cut(s) 69, 227
MboI GATC 2 cut(s) 67, 225
MboII GAAGA 2 cut(s) 177, 194
MflI RGATCY 1 cut(s) 67
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 1 cut(s) 39
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 6 cut(s) 224, 415, 456, 471, 480, 507
MseI TTAA 4 cut(s) 282, 354, 576, 587
MspA1I CMGCKG 1 cut(s) 348
MspCI CTTAAG 2 cut(s) 281, 575
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
MwoI GCNNNNNNNGC 1 cut(s) 394
NdeII GATC 2 cut(s) 67, 225
NlaIII CATG 2 cut(s) 315, 451
NmuCI GTSAC 1 cut(s) 499
NspI RCATGY 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 207
PcsI WCGNNNNNNNCGW 2 cut(s) 175, 258
PfeI GAWTC 1 cut(s) 180
PkrI GCNGC 4 cut(s) 139, 310, 350, 419
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 430
PspFI CCCAGC 1 cut(s) 344
PspGI CCWGG 1 cut(s) 430
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 1 cut(s) 67
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 4 cut(s) 282, 354, 576, 587
SatI GCNGC 4 cut(s) 138, 309, 349, 418
Sau3AI GATC 2 cut(s) 67, 225
ScaI AGTACT 1 cut(s) 132
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 432
SduI GDGCHC 1 cut(s) 252
SetI ASST 9 cut(s) 26, 202, 282, 291, 419, 462, 467, 511, 597
SfaNI GCATC 2 cut(s) 90, 165
SfcI CTRYAG 2 cut(s) 85, 100
SmlI CTYRAG 2 cut(s) 281, 575
SmoI CTYRAG 2 cut(s) 281, 575
Sse9I AATT 1 cut(s) 39
SsiI CCGC 4 cut(s) 15, 137, 348, 491
SspI AATATT 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 430
TaaI ACNGT 3 cut(s) 152, 262, 505
TasI AATT 1 cut(s) 39
TatI WGTACW 1 cut(s) 130
TauI GCSGC 2 cut(s) 140, 351
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 4 cut(s) 282, 354, 576, 587
Tru9I TTAA 4 cut(s) 282, 354, 576, 587
TscAI CASTG 4 cut(s) 78, 157, 328, 334
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 2 cut(s) 308, 417
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 1 cut(s) 38
TspRI CASTG 4 cut(s) 78, 157, 328, 334
Vha464I CTTAAG 2 cut(s) 281, 575
XagI CCTNNNNNAGG 1 cut(s) 578
XceI RCATGY 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 103
ZrmI AGTACT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.