Rw2G017460
BHLH Family

Transcription factor bHLH131-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005834
Physical Location & Seq
Forward (+)
21538118 .. 21538975
858 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G017460.1

Sequence Viewer

Length: 639 bp
ATGTGTTCCGCCCCAAGCTATTTTTCCAAGCAAATTGGTTTTCATCAACGCTATCGTATAGATCCAGTGAATAGCGTCTACAGAAAAAACTTCTGTAGACCAAAATCAACAGCAGAAGCCAAAGTACTTGCGGCCAAGAAACACAGTGAGTCAGAGAGAAGACGAAGGATGCGAATCAATAGTCAATATGCAGCTCTTCGTAAAATCCTCCCAAACTTGATCAAAATGGACAAGGCTTCTTTGCTCGCAGAGACCGTCCGACAAGTAAGGGAGCTTAAGAAGGTGGTAGCAGATACTGAAGCAGCATGTCGTGGCAGTGGCAGTGAGTGTGTTATTCCCAGCGGCGTTAACAAGTTGAGTTTCGAGAAGTGTGAAGGCAAGCAAGAAGGGCTTGTGAAAGCAACATTTAGCTGCGAGGATAGGCCAGGGCTAATATTGGACATGATTAGGGAGCTGAGGTCAGGGAAAGGGAGGGTGGTGAGGGCGGAGATGGTGACAGTAGGTGGGAGGACTAAGAGTGTGTTGTGGGTGAAAGGGTTGGGTGCTGGAAGTGAAGGGATAGTGTGCCTTAAGAAGGCATTAAATAAGGTGATTGTTGACAGGCCAAGCATTATTCAGAAGCTACATCTCCCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

212

Amino Acids

23.63

Weight (kDa)

10.11

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 46 - 92 1.1e-08 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 78, 97
AciI CCGC 4 cut(s) 9, 131, 342, 485
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 132
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 574
AflII CTTAAG 2 cut(s) 275, 569
AjnI CCWGG 1 cut(s) 424
AjuI GAANNNNNNNTTGG 2 cut(s) 7, 39
AluBI AGCT 6 cut(s) 18, 194, 274, 411, 454, 622
AluI AGCT 6 cut(s) 18, 194, 274, 411, 454, 622
Alw26I GTCTC 1 cut(s) 245
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 3 cut(s) 132, 422, 602
ApeKI GCWGC 3 cut(s) 191, 302, 411
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
AsuHPI GGTGA 4 cut(s) 490, 505, 541, 601
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
BbsI GAAGAC 1 cut(s) 166
BbvCI CCTCAGC 1 cut(s) 455
BbvI GCAGC 3 cut(s) 203, 314, 398
BccI CCATC 1 cut(s) 484
BciT130I CCWGG 1 cut(s) 426
BclI TGATCA 1 cut(s) 219
BcoDI GTCTC 1 cut(s) 245
BfmI CTRYAG 2 cut(s) 79, 94
BfrI CTTAAG 2 cut(s) 275, 569
BisI GCNGC 5 cut(s) 132, 192, 303, 343, 412
BlsI GCNGC 5 cut(s) 133, 193, 304, 344, 413
BmcAI AGTACT 1 cut(s) 126
Bme1390I CCNGG 1 cut(s) 426
BmrFI CCNGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 166
Bpu10I CCTNAGC 1 cut(s) 455
BsaBI GATNNNNATC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 425
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse1I ACTGG 1 cut(s) 65
Bse8I GATNNNNATC 1 cut(s) 173
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 425
BseGI GGATG 1 cut(s) 174
BseJI GATNNNNATC 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 574
BseMII CTCAG 1 cut(s) 446
BseNI ACTGG 1 cut(s) 65
BseXI GCAGC 3 cut(s) 203, 314, 398
BseYI CCCAGC 1 cut(s) 338
BshFI GGCC 3 cut(s) 134, 424, 604
BslI CCNNNNNNNGG 1 cut(s) 574
BsmAI GTCTC 1 cut(s) 245
BsnI GGCC 3 cut(s) 134, 424, 604
Bso31I GGTCTC 1 cut(s) 245
Bsp143I GATC 2 cut(s) 61, 219
BspACI CCGC 4 cut(s) 9, 131, 342, 485
BspANI GGCC 3 cut(s) 134, 424, 604
BspCNI CTCAG 1 cut(s) 447
BspPI GGATC 1 cut(s) 56
BspQI GCTCTTC 1 cut(s) 201
BspTI CTTAAG 2 cut(s) 275, 569
BspTNI GGTCTC 1 cut(s) 245
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 1 cut(s) 425
BssMI GATC 2 cut(s) 61, 219
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 3 cut(s) 146, 256, 499
Bst6I CTCTTC 1 cut(s) 201
BstAFI CTTAAG 2 cut(s) 275, 569
BstC8I GCNNGC 2 cut(s) 246, 380
BstDEI CTNAG 3 cut(s) 455, 513, 633
BstENI CCTNNNNNAGG 1 cut(s) 572
BstF5I GGATG 1 cut(s) 174
BstKTI GATC 2 cut(s) 64, 222
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 2 cut(s) 61, 219
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 2 cut(s) 79, 94
BstV1I GCAGC 3 cut(s) 203, 314, 398
BstV2I GAAGAC 1 cut(s) 166
BstX2I RGATCY 1 cut(s) 61
BstYI RGATCY 1 cut(s) 61
BsuRI GGCC 3 cut(s) 134, 424, 604
BtsCI GGATG 1 cut(s) 174
BtsI GCAGTG 2 cut(s) 322, 328
BtsIMutI CAGTG 4 cut(s) 72, 151, 322, 328
Cac8I GCNNGC 2 cut(s) 246, 380
CaiI CAGNNNCTG 1 cut(s) 296
CseI GACGC 1 cut(s) 64
Csp6I GTAC 1 cut(s) 125
CviAII CATG 2 cut(s) 306, 442
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 3 cut(s) 455, 513, 633
DpnI GATC 2 cut(s) 63, 221
DpnII GATC 2 cut(s) 61, 219
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 500
Eco31I GGTCTC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 318
EcoNI CCTNNNNNAGG 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 424
FaeI CATG 2 cut(s) 309, 445
FaiI YATR 4 cut(s) 59, 189, 307, 443
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FatI CATG 2 cut(s) 305, 441
FbaI TGATCA 1 cut(s) 219
FblI GTMKAC 2 cut(s) 78, 97
Fnu4HI GCNGC 5 cut(s) 132, 192, 303, 343, 412
FokI GGATG 1 cut(s) 181
Fsp4HI GCNGC 5 cut(s) 132, 192, 303, 343, 412
GluI GCNGC 5 cut(s) 132, 192, 303, 343, 412
GsaI CCCAGC 1 cut(s) 342
HaeIII GGCC 3 cut(s) 134, 424, 604
HgaI GACGC 1 cut(s) 64
Hin1II CATG 2 cut(s) 309, 445
HincII GTYRAC 2 cut(s) 349, 598
HindII GTYRAC 2 cut(s) 349, 598
HinfI GANTC 2 cut(s) 149, 174
HpaI GTTAAC 1 cut(s) 349
HphI GGTGA 4 cut(s) 490, 505, 541, 601
Hpy166II GTNNAC 4 cut(s) 79, 98, 349, 598
Hpy188I TCNGA 3 cut(s) 154, 260, 618
Hpy188III TCNNGA 1 cut(s) 364
Hpy8I GTNNAC 4 cut(s) 79, 98, 349, 598
HpyAV CCTTC 6 cut(s) 159, 274, 368, 380, 548, 568
HpyCH4III ACNGT 3 cut(s) 146, 256, 499
HpyCH4V TGCA 1 cut(s) 191
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 3 cut(s) 455, 513, 633
Hsp92II CATG 2 cut(s) 309, 445
Ksp22I TGATCA 1 cut(s) 219
KspAI GTTAAC 1 cut(s) 349
Kzo9I GATC 2 cut(s) 61, 219
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 2 cut(s) 271, 451
LpnPI CCDG 7 cut(s) 78, 352, 411, 438, 447, 531, 586
Lsp1109I GCAGC 3 cut(s) 203, 314, 398
LweI GCATC 1 cut(s) 159
MaeIII GTNAC 1 cut(s) 493
MalI GATC 2 cut(s) 63, 221
MboI GATC 2 cut(s) 61, 219
MboII GAAGA 2 cut(s) 171, 188
MflI RGATCY 1 cut(s) 61
MluCI AATT 1 cut(s) 33
MlyI GAGTC 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 6 cut(s) 218, 409, 450, 465, 474, 501
MseI TTAA 4 cut(s) 276, 348, 570, 581
MspA1I CMGCKG 1 cut(s) 342
MspCI CTTAAG 2 cut(s) 275, 569
MspR9I CCNGG 1 cut(s) 426
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 1 cut(s) 388
NdeII GATC 2 cut(s) 61, 219
NlaIII CATG 2 cut(s) 309, 445
NmuCI GTSAC 1 cut(s) 493
NspI RCATGY 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 201
PcsI WCGNNNNNNNCGW 2 cut(s) 169, 252
PfeI GAWTC 1 cut(s) 174
PkrI GCNGC 5 cut(s) 133, 193, 304, 344, 413
PleI GAGTC 1 cut(s) 157
PpsI GAGTC 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 424
PspFI CCCAGC 1 cut(s) 338
PspGI CCWGG 1 cut(s) 424
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 61
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 4 cut(s) 276, 348, 570, 581
SatI GCNGC 5 cut(s) 132, 192, 303, 343, 412
Sau3AI GATC 2 cut(s) 61, 219
ScaI AGTACT 1 cut(s) 126
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 426
SfaNI GCATC 1 cut(s) 159
SfcI CTRYAG 2 cut(s) 79, 94
SmlI CTYRAG 2 cut(s) 275, 569
SmoI CTYRAG 2 cut(s) 275, 569
Sse9I AATT 1 cut(s) 33
SsiI CCGC 4 cut(s) 9, 131, 342, 485
SspI AATATT 1 cut(s) 435
StyD4I CCNGG 1 cut(s) 424
TaaI ACNGT 3 cut(s) 146, 256, 499
TaqI TCGA 1 cut(s) 363
TasI AATT 1 cut(s) 33
TatI WGTACW 1 cut(s) 124
TauI GCSGC 2 cut(s) 134, 345
TfiI GAWTC 1 cut(s) 174
Tru1I TTAA 4 cut(s) 276, 348, 570, 581
Tru9I TTAA 4 cut(s) 276, 348, 570, 581
TscAI CASTG 4 cut(s) 72, 151, 322, 328
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 3 cut(s) 191, 302, 411
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 1 cut(s) 32
TspRI CASTG 4 cut(s) 72, 151, 322, 328
Vha464I CTTAAG 2 cut(s) 275, 569
XagI CCTNNNNNAGG 1 cut(s) 572
XceI RCATGY 1 cut(s) 309
XmiI GTMKAC 2 cut(s) 78, 97
ZrmI AGTACT 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.