RLG00000017888
BHLH Family

Transcription factor bHLH131-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005834
Physical Location & Seq
Forward (+)
22655397 .. 22656267
871 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017888

Sequence Viewer

Length: 651 bp
ATGCAGATGTGTTCCGCCCCAAGCTATTTTTCCAAGCAAATTGGCTTTCCTCAACGCTATCGTATAGATCCAGTGAATAGCATCTACAGCAAAAACGTCTGTAGACCAAAATCAACAGCAGAAGCCAAAGTACTTGCTGCCAAGAAACACAGTGAGTCAGAGAGAAGACGAAGGATGCGAATCAATAGTCAATATGCAGCTCTTCGTAAAATCCTCCCAAACTTGATCAAAATGGACAAGGCTTCTGTGCTTGCAGAGACTGTCCGACAAGTAAGGGAGCTTAAGAAGGTGGTAGCAGATACTGAAGCAGCATGTCGTGGCAGTGGCAGTGGCAGTGAGTGTGTTATTCCCAGTGGCGTTAACAAGTTGAGTTTGGAGAAGTGTGAAGGCAAGCGAGAAAGGCTTGTGAAAGCAACATTTAGCTGCGAGGATAGGCCAGGGCTAATATTGGACATGATTAGGGAGCTGAGGTCAGGGAAAGGGAGGGTGGTGAGGGCGGAGATGGTGACAGTAGGGGGGAGGACTAAGAGTGTGTTGTGGGTGAAAGGGTTGGGTGCTGGAAGTGAAGGGATAGTGTGCCTTAAGAAGGCATTAAATAAGGTGATTGTTGACAGGCCAAGCATTATTCAGAAGCTACGTCCCCCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

217

Amino Acids

23.97

Weight (kDa)

10.23

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 48 - 94 2.3e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 103
AciI CCGC 2 cut(s) 15, 497
AclWI GGATC 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 586
AflII CTTAAG 2 cut(s) 281, 581
AjnI CCWGG 1 cut(s) 436
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 6 cut(s) 24, 200, 280, 423, 466, 634
AluI AGCT 6 cut(s) 24, 200, 280, 423, 466, 634
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 62
AlwNI CAGNNNCTG 2 cut(s) 260, 302
AoxI GGCC 2 cut(s) 434, 614
ApeKI GCWGC 4 cut(s) 137, 197, 308, 423
ArsI GACNNNNNNTTYG 2 cut(s) 172, 204
AsuHPI GGTGA 4 cut(s) 502, 517, 553, 613
BarI GAAGNNNNNNTAC 2 cut(s) 114, 146
BbsI GAAGAC 1 cut(s) 172
BbvCI CCTCAGC 1 cut(s) 467
BbvI GCAGC 4 cut(s) 124, 209, 320, 410
BccI CCATC 1 cut(s) 496
BciT130I CCWGG 1 cut(s) 438
BclI TGATCA 1 cut(s) 225
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 2 cut(s) 85, 100
BfrI CTTAAG 2 cut(s) 281, 581
BisI GCNGC 4 cut(s) 138, 198, 309, 424
BlsI GCNGC 4 cut(s) 139, 199, 310, 425
BmcAI AGTACT 1 cut(s) 132
Bme1390I CCNGG 1 cut(s) 438
BmrFI CCNGG 1 cut(s) 438
BmrI ACTGGG 1 cut(s) 345
BmsI GCATC 2 cut(s) 90, 165
BmuI ACTGGG 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 467
BsaBI GATNNNNATC 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 455, 485
Bsc4I CCNNNNNNNGG 1 cut(s) 586
Bse1I ACTGG 2 cut(s) 71, 351
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 437
BseGI GGATG 1 cut(s) 180
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 586
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 2 cut(s) 71, 351
BseXI GCAGC 4 cut(s) 124, 209, 320, 410
BshFI GGCC 2 cut(s) 436, 616
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 1 cut(s) 586
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 624
BsnI GGCC 2 cut(s) 436, 616
Bsp143I GATC 2 cut(s) 67, 225
BspACI CCGC 2 cut(s) 15, 497
BspANI GGCC 2 cut(s) 436, 616
BspCNI CTCAG 1 cut(s) 459
BspPI GGATC 1 cut(s) 62
BspQI GCTCTTC 1 cut(s) 207
BspTI CTTAAG 2 cut(s) 281, 581
BsrI ACTGG 2 cut(s) 71, 351
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 2 cut(s) 67, 225
Bst2UI CCWGG 1 cut(s) 438
Bst4CI ACNGT 3 cut(s) 152, 262, 511
Bst6I CTCTTC 1 cut(s) 207
BstAFI CTTAAG 2 cut(s) 281, 581
BstC8I GCNNGC 2 cut(s) 252, 392
BstDEI CTNAG 3 cut(s) 467, 525, 645
BstENI CCTNNNNNAGG 1 cut(s) 584
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 2 cut(s) 70, 228
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 2 cut(s) 67, 225
BstMWI GCNNNNNNNGC 2 cut(s) 87, 400
BstNI CCWGG 1 cut(s) 438
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 436
BstSFI CTRYAG 2 cut(s) 85, 100
BstV1I GCAGC 4 cut(s) 124, 209, 320, 410
BstV2I GAAGAC 1 cut(s) 172
BstX2I RGATCY 1 cut(s) 67
BstYI RGATCY 1 cut(s) 67
BsuRI GGCC 2 cut(s) 436, 616
BtsCI GGATG 1 cut(s) 180
BtsI GCAGTG 3 cut(s) 328, 334, 340
BtsIMutI CAGTG 6 cut(s) 78, 157, 328, 334, 340, 358
Cac8I GCNNGC 2 cut(s) 252, 392
CaiI CAGNNNCTG 2 cut(s) 260, 302
Csp6I GTAC 1 cut(s) 131
CviAII CATG 2 cut(s) 312, 454
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 3 cut(s) 467, 525, 645
DpnI GATC 2 cut(s) 69, 227
DpnII GATC 2 cut(s) 67, 225
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
EciI GGCGGA 2 cut(s) 4, 512
Eco57I CTGAAG 1 cut(s) 324
EcoNI CCTNNNNNAGG 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 436
FaeI CATG 2 cut(s) 315, 457
FaiI YATR 4 cut(s) 65, 195, 313, 455
FaqI GGGAC 1 cut(s) 624
FatI CATG 2 cut(s) 311, 453
FbaI TGATCA 1 cut(s) 225
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 4 cut(s) 138, 198, 309, 424
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 4 cut(s) 138, 198, 309, 424
GluI GCNGC 4 cut(s) 138, 198, 309, 424
HaeIII GGCC 2 cut(s) 436, 616
Hin1II CATG 2 cut(s) 315, 457
HincII GTYRAC 2 cut(s) 361, 610
HindII GTYRAC 2 cut(s) 361, 610
HinfI GANTC 2 cut(s) 155, 180
HpaI GTTAAC 1 cut(s) 361
HphI GGTGA 4 cut(s) 502, 517, 553, 613
Hpy166II GTNNAC 3 cut(s) 104, 361, 610
Hpy188I TCNGA 3 cut(s) 160, 266, 630
Hpy8I GTNNAC 3 cut(s) 104, 361, 610
HpyAV CCTTC 5 cut(s) 165, 280, 380, 560, 580
HpyCH4III ACNGT 3 cut(s) 152, 262, 511
HpyCH4IV ACGT 2 cut(s) 96, 637
HpyCH4V TGCA 3 cut(s) 4, 197, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 400
HpyF3I CTNAG 3 cut(s) 467, 525, 645
HpySE526I ACGT 2 cut(s) 96, 637
Hsp92II CATG 2 cut(s) 315, 457
Ksp22I TGATCA 1 cut(s) 225
KspAI GTTAAC 1 cut(s) 361
Kzo9I GATC 2 cut(s) 67, 225
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 2 cut(s) 277, 463
LpnPI CCDG 7 cut(s) 84, 364, 423, 450, 459, 543, 598
Lsp1109I GCAGC 4 cut(s) 124, 209, 320, 410
LweI GCATC 2 cut(s) 90, 165
MaeII ACGT 2 cut(s) 96, 637
MaeIII GTNAC 1 cut(s) 505
MalI GATC 2 cut(s) 69, 227
MboI GATC 2 cut(s) 67, 225
MboII GAAGA 2 cut(s) 177, 194
MflI RGATCY 1 cut(s) 67
MluCI AATT 1 cut(s) 39
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 289
MnlI CCTC 7 cut(s) 60, 224, 421, 462, 477, 486, 513
MseI TTAA 4 cut(s) 282, 360, 582, 593
MspCI CTTAAG 2 cut(s) 281, 581
MspR9I CCNGG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 438
MwoI GCNNNNNNNGC 2 cut(s) 87, 400
NdeII GATC 2 cut(s) 67, 225
NlaIII CATG 2 cut(s) 315, 457
NmuCI GTSAC 1 cut(s) 505
NspI RCATGY 1 cut(s) 315
PciSI GCTCTTC 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 175
PfeI GAWTC 1 cut(s) 180
PkrI GCNGC 4 cut(s) 139, 199, 310, 425
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 436
PspGI CCWGG 1 cut(s) 436
PstNI CAGNNNCTG 2 cut(s) 260, 302
PsuI RGATCY 1 cut(s) 67
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 4 cut(s) 282, 360, 582, 593
SatI GCNGC 4 cut(s) 138, 198, 309, 424
Sau3AI GATC 2 cut(s) 67, 225
ScaI AGTACT 1 cut(s) 132
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 438
SfaNI GCATC 2 cut(s) 90, 165
SfcI CTRYAG 2 cut(s) 85, 100
SmlI CTYRAG 2 cut(s) 281, 581
SmoI CTYRAG 2 cut(s) 281, 581
Sse9I AATT 1 cut(s) 39
SsiI CCGC 2 cut(s) 15, 497
SspI AATATT 1 cut(s) 447
StyD4I CCNGG 1 cut(s) 436
TaaI ACNGT 3 cut(s) 152, 262, 511
TaiI ACGT 2 cut(s) 99, 640
TasI AATT 1 cut(s) 39
TatI WGTACW 1 cut(s) 130
TfiI GAWTC 1 cut(s) 180
Tru1I TTAA 4 cut(s) 282, 360, 582, 593
Tru9I TTAA 4 cut(s) 282, 360, 582, 593
TscAI CASTG 6 cut(s) 78, 157, 328, 334, 340, 358
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 4 cut(s) 137, 197, 308, 423
Tsp45I GTSAC 1 cut(s) 505
TspRI CASTG 6 cut(s) 78, 157, 328, 334, 340, 358
Vha464I CTTAAG 2 cut(s) 281, 581
XagI CCTNNNNNAGG 1 cut(s) 584
XceI RCATGY 1 cut(s) 315
XmiI GTMKAC 1 cut(s) 103
ZrmI AGTACT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.