Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Reverse (-)

12413856 .. 12421514

Gene Structure 7659 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g20200.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5187 bp

ATGAGAGGATCACACCCACACCCGCCGTACCGGCAGCCGCAGCCGGCCGGAATTCCGCGGCGGTATCCACAGCAGCGGCAGTTTTATCCAGGATACTCACGCGCTCCTCCGCCTCCGCAGCGGCCGTCGCTCCCAATTGAGCGCCCGAATTTTGTCGTGGACTTGCTCTCCGACCGCCGCGACCTCTCGGAGTCGTCCGTCGAGAAGGTGATCAAGCAGTGCAGGGCGACGCCGGAGAGTTTCCGCGTGAGCCCGACGAACCTCATCGCCGCGTCGCTGTACTACTCGCAGTGGTACGATGCGCTTGAGGCGATCGTCTGGCTGTGGGAGTCGCGGCTCGACCGGGTTCACAGATTCATGCCCAAGCTCGACGCCAAGGTCTCGGTGCCGTCGGACAGCGTAGAGCTCGAGGACCGCCTGAAGGCCTTGTTCGCCGACCGAATTAGGCTCTTGATGGGCGGCGACGAGGTGAAGAAATGTGAAGAGAAGCGCCAGAATTTGGCGAGGGAGTACGAGAGAGTTCACAAGCTGTCTAAGAGGCCACAGAAGTATTGGGAGGACCTAGCGGGGAAGGAGGAGAGGTGCAAAGGGGAACTTGAATTGGTGGAGAGTAGGATTAGAGAGTTCAGGTCTGGTATGAACTGTTTGCTTGCTCATGTCGAAGGGAAAGAGTTGGGGGATTATGGTGAAGAAGGGATGAAGCTGTTTAAATTCGGCGAAATTCGGGATTGGAGTAAAATTCAGAGTTATATGACGAGGGAGTGCCGCAGACTCGAAGAGGGATTGCCTATTTATGCTCATCGGCAGCAAATTCTTGAGCAGATTAATAATCAGCAGGTCATGGTGCTGATTGGAGAGACTGGTTCGGGGAAGAGTACACAGTTGGTTCAGTTTCTTGCTGATTCTGGAATCGCTGCTGGTAGTTCCATTGTATGCACTCAGCCTCGCAAGATTGCTGCCAATTCACTGGCAAAGAGAGTTAAAGAAGAATGCAGTGGTTGTTATGGAGAAAACACAGTTACCAGTTATCAGCCATCATCTGGACAGAAGTCTGGACAGCAGTCTACTACTAAGGTAACATATATGACAGACCACTGTTTACTGCAGTGCTACATGAACGATACAAATTTGTCCAGGATGTCTTGCATCATAGTAGATGAGGCTCATGAAAGGACCTTGAGTACTGATCTCCTGTTAGCATTGATCAAAGATTTACTAAGTCGAAGGCCTCAGTTACGGCTAATTATAATGTCTGCAACAGCTGATGCAGAAGTGCTTTCACATTATTTTTTCAAGTGTAAAATCTTTCATGTAGTGGGGAGGAACTTTCCAGTTGATGTAAGATATGTTCCCCCTTTCACTGAAGGAACTGCCAGTAATGTTGCTTCTTATGTTTCTGATGTGATGAGAGTAGCACGAGAGATCCACAAAAATGAGAAAGAAGGGACAATTCTTGCATTTTTGACTTCCCAGATGGAAGTTGAATGGGTCTGCGAGAAGTTTATAACACCTGGTGCAATTGCATTGCCATTGCATGGGAAGCTTTCTTTTGAAGAACAGTTTAATGTTTTTCAAAATTTCCCTGGAAAACGAAAAATTATATTCGCAACAAATCTGGCGGAGACATCCCTGACAATTCCTGGGGTAAAGTATGTGATTGATTCTGGCATGGTCAAAGAAAGCAAATTTGAACCTGGAAGTGGTATGAATGTTCTCAGAGTTTGCTGGATCAGCCAGAGTTCTGCGAATCAAAGAACTGGGCGTGCTGGAAGAACGGGACCTGGAATATGCTATAGACTTTACTCAGAATATGATTTTCAGGCGATGCCTCCTTGTCAGGAGCCTGAGATCCGCAGAGTTCATCTCGGTGTAGCTGTTCTGAGGATTCTGGCTTTGGGTGTCAAGAACCTAAGAGAGTTTGAATTCATTGATGCACCTTGTTCTGAAGCTATAGACATGGCAATGAGGAATCTTGTTCAGTTAGGAGCTGTTAAGCAAAACAAGGATGTCTATGAACTGACTCTAGAAGGTCGCCGCTTGGTTAAATTGGGTGTTGAACCTAGGCTTGGTAAATTAATTCTTGGCTGCTGTAACTATAACTTGCGCAAGGAGGGCCTTGTTCTGGCGGCTGTAATGGCAAATGCAAGCAGCATATTTTGCAGGGTTGGTAATGATGAAGAGAAACTTAGGTCCGACTGCTTCAAGGTGAAATTTTGCCATCGTGATGGTGACCTCTTTACCCTTCTATCAGTGTACAAGCAATGGGAAGCTGAGCCTCGAGATCGGAAAAGCACGTGGTGTTGGGATAACAGCATCAATGCCAAAACTATGAGGAGATGCCATGACACTGTACAGGAATTGGAGTCTTGCCTTAAGCATGAACTCAGTATGATAATTCCTAGCAGCTGGCATTGGAATGAGGATGTTTCAACTGACTCTGATAAATTCTTGAAAAAGGTTATACTTTCTTCCCTGGCAGAAAATGTAGCAATGTTCTCTGGTTATGATCAAGTTGGTTATGAGGTGGCATTAACTGGGCAGCATGTTCGGCTACACCCTTCTTGTTCATTGCTGGTATTTGGTCAGAAACCTGGTTGGGTTGTTTTTGGTGAACTCCTGTCAAGTTCTAATCAGTATTTGACCTGTGTCACTTCCATCGATTTCAACGCTTTGTCTACCCTTGATCCCCCTCCTGTGTTTGATGTGTCCAAGATGGAAGGTCGAAAGTTGCAAGTGAAAGTGTTAACTGGTTTTGGCAGTTGCTTGCTTAAAAGATTTTGTGGTAAGGGCAACGGTTATCTGCATCATCTTGTGTCTCGCGTTAGGACATTTTGCAGTGACGAACTTATCAGTATCAAGGTCGATTATTATCAAAATGAGATTATGGTTTTTGCTACCTCACATAACATGGATACAGTTATAAACTTTGTATCTGATGCTTTGGAGTGTGAAAAAAGATGGTTGCGTAATGAATGTTTGGAGAAATGCTTGTATCATGGTTCTGGTGGCTTGCCTCCTGTTGCATTGTTTGGGGCCGGTGCAGAGATCAAGCATCTGGAGCTTCAGAAAAGGTTCTTGACTGTTGATGTATTTCATTCTAAGCTGGATGGCATGGATGATAAGGCGCTTCTTTGTGAGCTCGAAGAGTCTGCCTCTGGTAGTATCTGTGGTCATCATAAACTCCTCAGCACTGGCCAGGACAGTGTTGACAAAGGAAAGGGGGCACGGTTGACATTTCTTACTCCTGATGAAGCTCAGAAAGCCGTTGAACTAAATGAGTCTGAGTTTAAAGGTTCTATACTGAAGGTTGTTCCTTCACAGGTTGGAGGTGATCATAAAGTGTTTCCACTCCTTGCTGTTAGAGCTACTGTTTTATGGCCTCGCAGGCAAAGCAATGGGTTTGCTATTGTTAAATGTGACATGGATGATATTGGCTTCATGCTTGATGATTTTACTAACCTTGTAATCGGAGGAAGAAGTATTCGTTGTGAGCTTAGCAAAAGATACATGGACTCTCTTGTGATCAGTGGAATAAATAGAGATCTTTCTGAGAAAGAAATCCTTGATGTGTTAACCACTGCTACTAGTAGGACAATCCTGGACTTTTTTCTTGTGAGAGGGGATGCTGTAGAAAATCCGCCATGTGGTGCTTGTGAAGAATCACTGCTAAAAGAAATATCACCGTATATGCCTAAACAGTACTCCCATAGTAACTGCAGTGTTCAGGTGTTCCAGCCTGAACCAAAGAGTGTTTTCATGAAAGCTTTGATAACATTTGATGGAAGATTACACTTAGAGGCAGCAAAAGCTTTAGAACATTTGGAAGGGAAAGTATTGCCCGGCTTCCTTCCATGGCAAAAGATGAAGTGTCAGCAGTTGTTTCATAGCTCTCTGTCTTGTCCAGGCCCTGTTTATTGTGTGATAAAGAAGCAGCTAGATCCTTTGCTTGAAAGTTTTACGCATCTAAAAGGTGTGGAATGTAATCTCGAGGAGTATCCTAATGGTTCCTGCAGAATTAAGATTTCAGCCAATGCTACAAAAAAGATTGCAGATCTGAGAAGACGTGTTGAGGAGCTTGTGAAGGGGAAGACTATTGACCACCCAAGCCTCACTGCAACTGTTTTGCAGCTTCTGTTTTCCAGAGATGGCATCAGTCTAATGTACTCATTACAGCGAGAGACAGGAACATATATCATTTTCGACAGGCAGAAAATCAATGTACAGGTCTTTGGTTCTTCAGATAAAGTTGATATGGTCACACAGAAATTGGTTGAATCCCTTTTGAACATCCACGAAAGCAAGGCTCTTGAGGTGCGACTTCAAGGTAATGCTTTACCTCCTGAATTGATGAAAGAGGTTGTTAGCAGGTTTGGACCAGACCTTCGGGGGCTCAAAGAGAGGGTACCAGGGGCTGAATTTAGTCTCAATGTACGCCGTCAATCCATTCTCATCCAGGGAAGCAAGGAGATGAAGCAAAAAGTAGATGAGATTATTGATGAGGTTGCACAAATGGCTGGTACTAGTTTGACCAAGAGGATAAAAAGTGAAGCTGATTGTCCAATCTGTTTGTGTGACGTTGAAGATGGTTACCGGCTTGAGGACTGTGGTCACTTATTTTGTCGTTCGTGTTTGGTTGAGCAGTGCGAGTCTGCAATCCATAATCAAGATAGCTTTCCATTACGCTGCACACATGAGGGTTGCATGTCTCCAGTTTTGATCACAGATCTCAGATCGCTTCTATCAATTGAGAAGCTAGAAGATCTCTTCAGGGCATCATTGGGTTCATTTGTTGCTATGAGTTGCGGCACCTACAGGTTTTGCCCATCTCCTGACTGCTCTTCAATCTATCAGGTAGCGGCTCCTGGAAAAGAAGCTGAACCATTTGTTTGTGGTGCGTGCTATGGGGAGACGTGTACCATGTGCCATTTAGAACATCATCCCTACATGTCATGCAAGCAGTACAAGGAGTTCAAGGAAGATCCAGATTCTTCATTGAAGGAGTGGTGCAAAGGAAAAGAACATGTGAAGAGCTGCCCGGTATGCAAATATACAATTGAGAAGATAGATGGGTGCAACCATATAGAATGTCGGTGCGGGAAGCATATATGCTGGGTTTGTTTGGCCTATTATGGAAGCAGCGATGAATGTTATGGCCACTTGAGAAGTGTTCACTTGACTTACATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1729

Amino Acids

195.01

Weight (kDa)

7.29

Isoelectric Point (pI)

47.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	267 - 424	4.2e-12	DEAD/DEAH box helicase
Helicase_C	PF00271	472 - 593	6.3e-16	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	817 - 891	1.6e-09	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	993 - 1107	1.3e-46	DEAH11-like, RNA-binding domain
RRM_DEAH11	PF24637	1127 - 1207	3.2e-37	DEAH11 RRM domain
KH_DEAH11_1st	PF24638	1296 - 1360	1.9e-29	DEAH11 eukaryotic type KH-domain
KH_DEAH11_2nd	PF24641	1361 - 1434	1.2e-35	DEAH11 eukaryotic type KH-domain
KH_DEAH11	PF24471	1436 - 1503	1e-28	DEAH11-like, KH-domain
IBR	PF01485	1595 - 1651	1.7e-07	IBR domain, a half RING-finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1247, 1505, 2921
AasI	GACNNNNNNGTC	1 cut(s)	377
Acc16I	TGCGCA	1 cut(s)	2105
Acc36I	ACCTGC	2 cut(s)	826, 4356
Acc65I	GGTACC	1 cut(s)	4402
AccB1I	GGYRCC	3 cut(s)	385, 4402, 4805
AccB7I	CCANNNNNTGG	3 cut(s)	499, 1040, 1535
AccI	GTMKAC	2 cut(s)	1064, 2675
AccII	CGCG	7 cut(s)	58, 102, 180, 246, 272, 334, 2820
AclWI	GGATC	7 cut(s)	16, 1417, 1736, 1843, 2678, 3932, 4973
AcoI	YGGCCR	4 cut(s)	45, 122, 3193, 5152
AcuI	CTGAAG	7 cut(s)	440, 1383, 1965, 3047, 3323, 4221, 4750
AcvI	CACGTG	1 cut(s)	2295
AcyI	GRCGYC	2 cut(s)	230, 372
AdeI	CACNNNGTG	2 cut(s)	1514, 2298
AfiI	CCNNNNNNNGG	9 cut(s)	421, 499, 1040, 1535, 1700, 2066, 2122, 3644, 4597
AflII	CTTAAG	1 cut(s)	2372
AflIII	ACRYGT	4 cut(s)	4063, 4910, 4944, 5020
AhlI	ACTAGT	2 cut(s)	3584, 4520
AjiI	CACGTC	2 cut(s)	4064, 4911
AjuI	GAANNNNNNNTTGG	2 cut(s)	2049, 2081
Alw21I	GWGCWC	2 cut(s)	408, 3141
Alw26I	GTCTC	8 cut(s)	385, 851, 1616, 2820, 4172, 4427, 4710, 4901
AlwI	GGATC	7 cut(s)	16, 1417, 1736, 1843, 2678, 3932, 4973
AlwNI	CAGNNNCTG	3 cut(s)	3908, 4132, 4412
Ama87I	CYCGRG	3 cut(s)	407, 2277, 3986
ArsI	GACNNNNNNTTYG	2 cut(s)	4365, 4397
AseI	ATTAAT	2 cut(s)	825, 2075
Asp700I	GAANNNNTTC	1 cut(s)	3071
Asp718I	GGTACC	1 cut(s)	4402
AspA2I	CCTAGG	1 cut(s)	2060
AspLEI	GCGC	6 cut(s)	104, 144, 304, 492, 2106, 3127
AspS9I	GGNCC	9 cut(s)	412, 559, 1173, 1778, 2113, 2190, 3033, 3905, 4373
AsuC2I	CCSGG	3 cut(s)	344, 3840, 5036
AsuHPI	GGTGA	8 cut(s)	220, 481, 698, 2218, 2240, 2621, 3341, 3672
AvaI	CYCGRG	3 cut(s)	407, 2277, 3986
AvaII	GGWCC	6 cut(s)	412, 559, 1173, 1778, 2190, 4373
AvrII	CCTAGG	1 cut(s)	2060
BaeGI	GKGCMC	1 cut(s)	3226
BalI	TGGCCA	2 cut(s)	3195, 5154
BanI	GGYRCC	3 cut(s)	385, 4402, 4805
BanII	GRGCYC	4 cut(s)	254, 408, 3141, 4392
BarI	GAAGNNNNNNTAC	2 cut(s)	1369, 1401
BauI	CACGAG	1 cut(s)	1416
BbrPI	CACGTG	1 cut(s)	2295
BbsI	GAAGAC	2 cut(s)	4066, 4094
Bbv12I	GWGCWC	2 cut(s)	408, 3141
BbvCI	CCTCAGC	1 cut(s)	3185
BceAI	ACGGC	6 cut(s)	10, 109, 373, 1253, 3248, 4419
BcgI	CGANNNNNNTGC	4 cut(s)	2528, 2562, 3131, 3165
BciVI	GTATCC	4 cut(s)	75, 86, 2905, 4005
BclI	TGATCA	6 cut(s)	210, 1203, 2506, 3331, 3522, 4716
BcnI	CCSGG	3 cut(s)	344, 3840, 5036
BcoDI	GTCTC	8 cut(s)	385, 851, 1616, 2820, 4172, 4427, 4710, 4901
BcuI	ACTAGT	2 cut(s)	3584, 4520
BfaI	CTAG	8 cut(s)	563, 2024, 2061, 2400, 3585, 3935, 4521, 4754
BfmI	CTRYAG	7 cut(s)	1103, 1792, 1950, 3627, 3715, 4009, 4810
BfoI	RGCGCY	3 cut(s)	145, 493, 3128
BfrI	CTTAAG	1 cut(s)	2372
BfuAI	ACCTGC	2 cut(s)	826, 4356
BfuI	GTATCC	4 cut(s)	75, 86, 2905, 4005
BglI	GCCNNNNNGGC	2 cut(s)	31, 3385
BglII	AGATCT	4 cut(s)	3541, 4051, 4723, 4759
BlnI	CCTAGG	1 cut(s)	2060
BlpI	GCTNAGC	2 cut(s)	2271, 3494
BmcAI	AGTACT	2 cut(s)	1183, 3701
Bme18I	GGWCC	6 cut(s)	412, 559, 1173, 1778, 2190, 4373
BmeT110I	CYCGRG	3 cut(s)	407, 2277, 3986
BmgBI	CACGTC	2 cut(s)	4064, 4911
BmgT120I	GGNCC	9 cut(s)	412, 559, 1173, 1778, 2113, 2190, 3033, 3905, 4373
BmiI	GGNNCC	8 cut(s)	387, 1779, 1842, 3034, 4006, 4404, 4807, 4860
BmrI	ACTGGG	2 cut(s)	1767, 2544
BmuI	ACTGGG	2 cut(s)	1767, 2544
BoxI	GACNNNNGTC	4 cut(s)	1048, 1060, 2645, 4605
BpiI	GAAGAC	2 cut(s)	4066, 4094
BplI	GAGNNNNNCTC	4 cut(s)	1848, 1880, 3137, 3169
BpmI	CTGGAG	2 cut(s)	3077, 4692
Bpu10I	CCTNAGC	1 cut(s)	3185
Bpu1102I	GCTNAGC	2 cut(s)	2271, 3494
BpuEI	CTTGAG	6 cut(s)	326, 836, 1198, 4328, 4616, 5179
BpuMI	CCSGG	3 cut(s)	344, 3840, 5036
Bsa29I	ATCGAT	1 cut(s)	2658
BsaAI	YACGTR	1 cut(s)	2295
BsaBI	GATNNNNATC	1 cut(s)	2868
BsaHI	GRCGYC	2 cut(s)	230, 372
BsaI	GGTCTC	1 cut(s)	385
BsaJI	CCNNGG	9 cut(s)	56, 375, 1582, 1640, 2060, 2472, 3851, 4406, 4453
BsaXI	ACNNNNNCTCC	4 cut(s)	152, 182, 566, 596
Bsc4I	CCNNNNNNNGG	9 cut(s)	421, 499, 1040, 1535, 1700, 2066, 2122, 3644, 4597
Bse118I	RCCGGY	4 cut(s)	30, 43, 3035, 4590
Bse3DI	GCAATG	7 cut(s)	1523, 1529, 1968, 2267, 2496, 2567, 3400
Bse8I	GATNNNNATC	1 cut(s)	2868
BseCI	ATCGAT	1 cut(s)	2658
BseDI	CCNNGG	9 cut(s)	56, 375, 1582, 1640, 2060, 2472, 3851, 4406, 4453
BseJI	GATNNNNATC	1 cut(s)	2868
BseLI	CCNNNNNNNGG	9 cut(s)	421, 499, 1040, 1535, 1700, 2066, 2122, 3644, 4597
BseMI	GCAATG	7 cut(s)	1523, 1529, 1968, 2267, 2496, 2567, 3400
BseRI	GAGGAG	6 cut(s)	96, 590, 2347, 3173, 4004, 4085
BseSI	GKGCMC	1 cut(s)	3226
BseX3I	CGGCCG	2 cut(s)	45, 122
BseYI	CCCAGC	1 cut(s)	5109
BsgI	GTGCAG	3 cut(s)	241, 3060, 4669
Bsh1236I	CGCG	7 cut(s)	58, 102, 180, 246, 272, 334, 2820
Bsh1285I	CGRYCG	6 cut(s)	48, 125, 175, 315, 343, 439
BshNI	GGYRCC	3 cut(s)	385, 4402, 4805
BshVI	ATCGAT	1 cut(s)	2658
BsiEI	CGRYCG	6 cut(s)	48, 125, 175, 315, 343, 439
BsiHKAI	GWGCWC	2 cut(s)	408, 3141
BsiHKCI	CYCGRG	3 cut(s)	407, 2277, 3986
BsiSI	CCGG	9 cut(s)	31, 44, 48, 233, 343, 3036, 3840, 4591, 5036
BslFI	GGGAC	2 cut(s)	1459, 1791
BslI	CCNNNNNNNGG	9 cut(s)	421, 499, 1040, 1535, 1700, 2066, 2122, 3644, 4597
BsmAI	GTCTC	8 cut(s)	385, 851, 1616, 2820, 4172, 4427, 4710, 4901
BsmBI	CGTCTC	1 cut(s)	4901
BsmFI	GGGAC	2 cut(s)	1459, 1791
BsmI	GAATGC	1 cut(s)	995
Bso31I	GGTCTC	1 cut(s)	385
BsoBI	CYCGRG	3 cut(s)	407, 2277, 3986
Bsp1286I	GDGCHC	5 cut(s)	254, 408, 3141, 3226, 4392
Bsp1407I	TGTACA	3 cut(s)	2253, 2350, 4219
Bsp1720I	GCTNAGC	2 cut(s)	2271, 3494
Bsp19I	CCATGG	1 cut(s)	3851
BspDI	ATCGAT	1 cut(s)	2658
BspFNI	CGCG	7 cut(s)	58, 102, 180, 246, 272, 334, 2820
BspHI	TCATGA	2 cut(s)	1165, 3756
BspLI	GGNNCC	8 cut(s)	387, 1779, 1842, 3034, 4006, 4404, 4807, 4860
BspMAI	CTGCAG	3 cut(s)	1107, 3719, 4013
BspMI	ACCTGC	2 cut(s)	826, 4356
BspPI	GGATC	7 cut(s)	16, 1417, 1736, 1843, 2678, 3932, 4973
BspQI	GCTCTTC	2 cut(s)	4843, 5021
BspT107I	GGYRCC	3 cut(s)	385, 4402, 4805
BspTI	CTTAAG	1 cut(s)	2372
BspTNI	GGTCTC	1 cut(s)	385
BsrDI	GCAATG	7 cut(s)	1523, 1529, 1968, 2267, 2496, 2567, 3400
BsrFI	RCCGGY	4 cut(s)	30, 43, 3035, 4590
BsrGI	TGTACA	3 cut(s)	2253, 2350, 4219
BssAI	RCCGGY	4 cut(s)	30, 43, 3035, 4590
BssECI	CCNNGG	9 cut(s)	56, 375, 1582, 1640, 2060, 2472, 3851, 4406, 4453
BssNI	GRCGYC	2 cut(s)	230, 372
BssSI	CACGAG	1 cut(s)	1416
BssT1I	CCWWGG	3 cut(s)	375, 2060, 3851
Bst2BI	CACGAG	1 cut(s)	1416
Bst6I	CTCTTC	8 cut(s)	477, 771, 866, 2172, 3138, 4769, 4843, 5021
BstACI	GRCGYC	2 cut(s)	230, 372
BstAFI	CTTAAG	1 cut(s)	2372
BstAPI	GCANNNNNTGC	1 cut(s)	2157
BstAUI	TGTACA	3 cut(s)	2253, 2350, 4219
BstBAI	YACGTR	1 cut(s)	2295
BstDSI	CCRYGG	2 cut(s)	56, 3851
BstEII	GGTNACC	2 cut(s)	2228, 4586
BstFNI	CGCG	7 cut(s)	58, 102, 180, 246, 272, 334, 2820
BstH2I	RGCGCY	3 cut(s)	145, 493, 3128
BstHHI	GCGC	6 cut(s)	104, 144, 304, 492, 2106, 3127
BstMAI	GTCTC	8 cut(s)	385, 851, 1616, 2820, 4172, 4427, 4710, 4901
BstMCI	CGRYCG	6 cut(s)	48, 125, 175, 315, 343, 439
BstNSI	RCATGY	4 cut(s)	2546, 4705, 4948, 5024
BstPAI	GACNNNNGTC	4 cut(s)	1048, 1060, 2645, 4605
BstPI	GGTNACC	2 cut(s)	2228, 4586
BstSFI	CTRYAG	7 cut(s)	1103, 1792, 1950, 3627, 3715, 4009, 4810
BstSLI	GKGCMC	1 cut(s)	3226
BstUI	CGCG	7 cut(s)	58, 102, 180, 246, 272, 334, 2820
BstV2I	GAAGAC	2 cut(s)	4066, 4094
BstX2I	RGATCY	8 cut(s)	1422, 1848, 3541, 3937, 4051, 4723, 4759, 4978
BstXI	CCANNNNNNTGG	2 cut(s)	967, 2225
BstYI	RGATCY	8 cut(s)	1422, 1848, 3541, 3937, 4051, 4723, 4759, 4978
BstZI	CGGCCG	2 cut(s)	45, 122
Bsu15I	ATCGAT	1 cut(s)	2658
BsuI	GTATCC	4 cut(s)	75, 86, 2905, 4005
BsuTUI	ATCGAT	1 cut(s)	2658
BtgI	CCRYGG	2 cut(s)	56, 3851
BtgZI	GCGATG	3 cut(s)	250, 1838, 5154
BtrI	CACGTC	2 cut(s)	4064, 4911
BveI	ACCTGC	2 cut(s)	826, 4356
CaiI	CAGNNNCTG	3 cut(s)	3908, 4132, 4412
CciI	TCATGA	2 cut(s)	1165, 3756
CfoI	GCGC	6 cut(s)	104, 144, 304, 492, 2106, 3127
Cfr10I	RCCGGY	4 cut(s)	30, 43, 3035, 4590
Cfr13I	GGNCC	9 cut(s)	412, 559, 1173, 1778, 2113, 2190, 3033, 3905, 4373
Cfr42I	CCGCGG	1 cut(s)	59
ClaI	ATCGAT	1 cut(s)	2658
CseI	GACGC	3 cut(s)	238, 261, 380
CsiI	ACCWGGT	2 cut(s)	1510, 2590
DraI	TTTAAA	2 cut(s)	709, 3289
DraIII	CACNNNGTG	2 cut(s)	1514, 2298
DrdI	GACNNNNNNGTC	1 cut(s)	377
DseDI	GACNNNNNNGTC	1 cut(s)	377
EaeI	YGGCCR	4 cut(s)	45, 122, 3193, 5152
EagI	CGGCCG	2 cut(s)	45, 122
Eam1104I	CTCTTC	8 cut(s)	477, 771, 866, 2172, 3138, 4769, 4843, 5021
EarI	CTCTTC	8 cut(s)	477, 771, 866, 2172, 3138, 4769, 4843, 5021
EciI	GGCGGA	3 cut(s)	99, 1634, 3627
Ecl136II	GAGCTC	2 cut(s)	406, 3139
EclXI	CGGCCG	2 cut(s)	45, 122
Eco130I	CCWWGG	3 cut(s)	375, 2060, 3851
Eco147I	AGGCCT	2 cut(s)	425, 1228
Eco24I	GRGCYC	4 cut(s)	254, 408, 3141, 4392
Eco31I	GGTCTC	1 cut(s)	385
Eco47I	GGWCC	6 cut(s)	412, 559, 1173, 1778, 2190, 4373
Eco52I	CGGCCG	2 cut(s)	45, 122
Eco53kI	GAGCTC	2 cut(s)	406, 3139
Eco57I	CTGAAG	7 cut(s)	440, 1383, 1965, 3047, 3323, 4221, 4750
Eco72I	CACGTG	1 cut(s)	2295
Eco88I	CYCGRG	3 cut(s)	407, 2277, 3986
Eco91I	GGTNACC	2 cut(s)	2228, 4586
EcoICRI	GAGCTC	2 cut(s)	406, 3139
EcoO109I	RGGNCCY	5 cut(s)	559, 1173, 1778, 2113, 3905
EcoO65I	GGTNACC	2 cut(s)	2228, 4586
EcoRI	GAATTC	2 cut(s)	51, 1922
EcoT14I	CCWWGG	3 cut(s)	375, 2060, 3851
EcoT38I	GRGCYC	4 cut(s)	254, 408, 3141, 4392
ErhI	CCWWGG	3 cut(s)	375, 2060, 3851
Esp3I	CGTCTC	1 cut(s)	4901
FalI	AAGNNNNNCTT	6 cut(s)	579, 611, 2169, 2201, 3546, 3578
FaqI	GGGAC	2 cut(s)	1459, 1791
FauI	CCCGC	3 cut(s)	30, 559, 5087
FbaI	TGATCA	6 cut(s)	210, 1203, 2506, 3331, 3522, 4716
FblI	GTMKAC	2 cut(s)	1064, 2675
FriOI	GRGCYC	4 cut(s)	254, 408, 3141, 4392
FspBI	CTAG	8 cut(s)	563, 2024, 2061, 2400, 3585, 3935, 4521, 4754
FspI	TGCGCA	1 cut(s)	2105
GlaI	GCGC	6 cut(s)	103, 143, 303, 491, 2105, 3126
GsaI	CCCAGC	1 cut(s)	5113
GsuI	CTGGAG	2 cut(s)	3077, 4692
HaeII	RGCGCY	3 cut(s)	145, 493, 3128
HapII	CCGG	9 cut(s)	31, 44, 48, 233, 343, 3036, 3840, 4591, 5036
HgaI	GACGC	3 cut(s)	238, 261, 380
HhaI	GCGC	6 cut(s)	104, 144, 304, 492, 2106, 3127
Hin1I	GRCGYC	2 cut(s)	230, 372
Hin6I	GCGC	6 cut(s)	102, 142, 302, 490, 2104, 3125
HinP1I	GCGC	6 cut(s)	102, 142, 302, 490, 2104, 3125
HincII	GTYRAC	4 cut(s)	2745, 3208, 3231, 3573
HindII	GTYRAC	4 cut(s)	2745, 3208, 3231, 3573
HindIII	AAGCTT	3 cut(s)	1541, 3762, 3807
HpaI	GTTAAC	2 cut(s)	2745, 3573
HpaII	CCGG	9 cut(s)	31, 44, 48, 233, 343, 3036, 3840, 4591, 5036
HphI	GGTGA	8 cut(s)	220, 481, 698, 2218, 2240, 2621, 3341, 3672
Hpy99I	CGWCG	8 cut(s)	130, 203, 232, 259, 277, 374, 394, 467
HpyCH4IV	ACGT	4 cut(s)	2294, 4063, 4575, 4910
HpySE526I	ACGT	4 cut(s)	2294, 4063, 4575, 4910
Hsp92I	GRCGYC	2 cut(s)	230, 372
HspAI	GCGC	6 cut(s)	102, 142, 302, 490, 2104, 3125
KpnI	GGTACC	1 cut(s)	4406
KroI	GCCGGC	1 cut(s)	43
KroNI	GCCGGC	1 cut(s)	45
Ksp22I	TGATCA	6 cut(s)	210, 1203, 2506, 3331, 3522, 4716
KspAI	GTTAAC	2 cut(s)	2745, 3573
KspI	CCGCGG	1 cut(s)	59
LguI	GCTCTTC	2 cut(s)	4843, 5021
LmnI	GCTCC	7 cut(s)	109, 135, 1840, 1985, 3058, 4072, 4864
MabI	ACCWGGT	2 cut(s)	1510, 2590
MaeI	CTAG	8 cut(s)	563, 2024, 2061, 2400, 3585, 3935, 4521, 4754
MaeII	ACGT	4 cut(s)	2294, 4063, 4575, 4910
MfeI	CAATTG	4 cut(s)	135, 1518, 4743, 5052
MflI	RGATCY	8 cut(s)	1422, 1848, 3541, 3937, 4051, 4723, 4759, 4978
MhlI	GDGCHC	5 cut(s)	254, 408, 3141, 3226, 4392
MlsI	TGGCCA	2 cut(s)	3195, 5154
MluNI	TGGCCA	2 cut(s)	3195, 5154
MmeI	TCCRAC	4 cut(s)	195, 372, 2217, 3304
Mox20I	TGGCCA	2 cut(s)	3195, 5154
MroNI	GCCGGC	1 cut(s)	43
MroXI	GAANNNNTTC	1 cut(s)	3071
MscI	TGGCCA	2 cut(s)	3195, 5154
MslI	CAYNNNNRTG	5 cut(s)	1431, 1866, 1961, 2223, 2415
Msp20I	TGGCCA	2 cut(s)	3195, 5154
MspA1I	CMGCKG	5 cut(s)	58, 76, 121, 1262, 2406
MspCI	CTTAAG	1 cut(s)	2372
MspI	CCGG	9 cut(s)	31, 44, 48, 233, 343, 3036, 3840, 4591, 5036
MunI	CAATTG	4 cut(s)	135, 1518, 4743, 5052
Mva1269I	GAATGC	1 cut(s)	995
MvnI	CGCG	7 cut(s)	58, 102, 180, 246, 272, 334, 2820
NaeI	GCCGGC	1 cut(s)	45
NciI	CCSGG	3 cut(s)	344, 3840, 5036
NcoI	CCATGG	1 cut(s)	3851
NgoMIV	GCCGGC	1 cut(s)	43
NlaIV	GGNNCC	8 cut(s)	387, 1779, 1842, 3034, 4006, 4404, 4807, 4860
NmuCI	GTSAC	7 cut(s)	2228, 2647, 2837, 3416, 4255, 4571, 4607
NsbI	TGCGCA	1 cut(s)	2105
NspI	RCATGY	4 cut(s)	2546, 4705, 4948, 5024
PaeR7I	CTCGAG	3 cut(s)	407, 2277, 3986
PagI	TCATGA	2 cut(s)	1165, 3756
PceI	AGGCCT	2 cut(s)	425, 1228
PciI	ACATGT	2 cut(s)	4944, 5020
PciSI	GCTCTTC	2 cut(s)	4843, 5021
PctI	GAATGC	1 cut(s)	995
PdiI	GCCGGC	1 cut(s)	45
PdmI	GAANNNNTTC	1 cut(s)	3071
PflMI	CCANNNNNTGG	3 cut(s)	499, 1040, 1535
PfoI	TCCNGGA	4 cut(s)	88, 1133, 3597, 4861
Ple19I	CGATCG	1 cut(s)	315
PmaCI	CACGTG	1 cut(s)	2295
PmlI	CACGTG	1 cut(s)	2295
Ppu21I	YACGTR	1 cut(s)	2295
PpuMI	RGGWCCY	3 cut(s)	559, 1173, 1778
PscI	ACATGT	2 cut(s)	4944, 5020
PshAI	GACNNNNGTC	4 cut(s)	1048, 1060, 2645, 4605
PshBI	ATTAAT	2 cut(s)	825, 2075
PsiI	TTATAA	3 cut(s)	1247, 1505, 2921
Psp124BI	GAGCTC	2 cut(s)	408, 3141
Psp5II	RGGWCCY	3 cut(s)	559, 1173, 1778
PspCI	CACGTG	1 cut(s)	2295
PspEI	GGTNACC	2 cut(s)	2228, 4586
PspFI	CCCAGC	1 cut(s)	5109
PspN4I	GGNNCC	8 cut(s)	387, 1779, 1842, 3034, 4006, 4404, 4807, 4860
PspPI	GGNCC	9 cut(s)	412, 559, 1173, 1778, 2113, 2190, 3033, 3905, 4373
PspPPI	RGGWCCY	3 cut(s)	559, 1173, 1778
PspXI	VCTCGAGB	1 cut(s)	407
PsrI	GAACNNNNNNTAC	4 cut(s)	1332, 1364, 4952, 4984
PstI	CTGCAG	3 cut(s)	1107, 3719, 4013
PstNI	CAGNNNCTG	3 cut(s)	3908, 4132, 4412
PsuI	RGATCY	8 cut(s)	1422, 1848, 3541, 3937, 4051, 4723, 4759, 4978
PvuI	CGATCG	1 cut(s)	315
PvuII	CAGCTG	2 cut(s)	1262, 2406
RseI	CAYNNNNRTG	5 cut(s)	1431, 1866, 1961, 2223, 2415
SacI	GAGCTC	2 cut(s)	408, 3141
SacII	CCGCGG	1 cut(s)	59
SapI	GCTCTTC	2 cut(s)	4843, 5021
Sau96I	GGNCC	9 cut(s)	412, 559, 1173, 1778, 2113, 2190, 3033, 3905, 4373
ScaI	AGTACT	2 cut(s)	1183, 3701
SduI	GDGCHC	5 cut(s)	254, 408, 3141, 3226, 4392
SexAI	ACCWGGT	2 cut(s)	1510, 2590
SfcI	CTRYAG	7 cut(s)	1103, 1792, 1950, 3627, 3715, 4009, 4810
Sfr274I	CTCGAG	3 cut(s)	407, 2277, 3986
Sfr303I	CCGCGG	1 cut(s)	59
SgrBI	CCGCGG	1 cut(s)	59
SinI	GGWCC	6 cut(s)	412, 559, 1173, 1778, 2190, 4373
SlaI	CTCGAG	3 cut(s)	407, 2277, 3986
SmiMI	CAYNNNNRTG	5 cut(s)	1431, 1866, 1961, 2223, 2415
SpeI	ACTAGT	2 cut(s)	3584, 4520
SseBI	AGGCCT	2 cut(s)	425, 1228
SspMI	CTAG	8 cut(s)	563, 2024, 2061, 2400, 3585, 3935, 4521, 4754
SstI	GAGCTC	2 cut(s)	408, 3141
StuI	AGGCCT	2 cut(s)	425, 1228
StyI	CCWWGG	3 cut(s)	375, 2060, 3851
TaiI	ACGT	4 cut(s)	2297, 4066, 4578, 4913
TaqII	GACCGA	1 cut(s)	453
TatI	WGTACW	9 cut(s)	279, 875, 1181, 2253, 2350, 3699, 4161, 4219, 4959
TseFI	GTSAC	7 cut(s)	2228, 2647, 2837, 3416, 4255, 4571, 4607
Tsp45I	GTSAC	7 cut(s)	2228, 2647, 2837, 3416, 4255, 4571, 4607
TspGWI	ACGGA	1 cut(s)	187
Van91I	CCANNNNNTGG	3 cut(s)	499, 1040, 1535
Vha464I	CTTAAG	1 cut(s)	2372
VpaK11BI	GGWCC	6 cut(s)	412, 559, 1173, 1778, 2190, 4373
VspI	ATTAAT	2 cut(s)	825, 2075
XbaI	TCTAGA	1 cut(s)	2023
XceI	RCATGY	4 cut(s)	2546, 4705, 4948, 5024
XcmI	CCANNNNNNNNNTGG	1 cut(s)	549
XhoI	CTCGAG	3 cut(s)	407, 2277, 3986
XmaJI	CCTAGG	1 cut(s)	2060
XmiI	GTMKAC	2 cut(s)	1064, 2675
XmnI	GAANNNNTTC	1 cut(s)	3071
XspI	CTAG	8 cut(s)	563, 2024, 2061, 2400, 3585, 3935, 4521, 4754
ZrmI	AGTACT	2 cut(s)	1183, 3701

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.