RchiOBHm_Chr2g0112281

Helicase associated domain (HA2) Add an annotation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0009423
Physical Location & Seq
Reverse (-)
24164939 .. 24171612
6674 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48577

Sequence Viewer

Length: 5148 bp
ATGAGGGGATCAAAGCCGGCCGGAATGTCTGAGCAGCGACGATTTTATCCGCAACCAGGGTCCCACCCACGCGCCGCCGTTCCGCGACGGCCGTTGCCTCGAATTCAGCGGCCGAATTTCATCGTTTATCTTCTGTCCGACAAACGGGACCTCCGGCGGCCGGACATCGAGGCGGTGATCAACCAGTGCAGGGCGAAGCCGGAGAATTTCCGGGTGAGTCCGGAGAACAACACAGTCGCGTCGATGTTCTACGCGCAGTGGTACGACGCGCTCGAAGCGATGGTCTGGCTGTGGGAGTCGCGGCTCGACCGGGTTCACAATTTCATGCCCAAGCTTGACGCCAACGACTCCGTCTCCACGCCGTCCGATAGCGTGGAGCTGGAGGACCGCCTGAGAGCCTTGTTCGCCGGCCGAATTAAGCAGTTAATCGACGGCGACGAGGTGAAGAAGTGTGAAGCAAAGCGCCAAATTTTGGCCAGAGAGTACGACCGAGTTCGTAAGCTGGCCAAGAGGCCTCAGAGGAATTGGGAGGACTTACCGGAGAAGGAGAAGAGGTGCAGAGTTGAGCTTGAATTGGTTGAGAGTAGGATCAGAGAGTTCAGGTCTGCTATGAACTGCTTGCTTGCTCATGTTGAAGGGAAAGAGTTGGAGGAGTATGGTGAAGAAGGCTTGAAGCTGTTTAAATTCGGCGAAATTCGGCCTTGGAGTCGAATTCAGAGGTTAATGGTGAGGGAGTGTCGCCGACTCGATGAGGGATTGCCCATTTATGCTCATAGGCAGCAAATTCTTGAGCAGATTACTAATCAGCAGGTCATGGTGTTGATTGGAGAGACTGGTTCAGGGAAGAGTACACAATTGGTTCAGTTTCTTGCTGATTCTGGAATCGCTGCTGATAAGTCCATTGTATGCACTCAGCCTCGCAAGATTGCTGCCAATTCATTGGCAAAGAGAGTCAAAGAAGAAAGTAGTGGGTGTTTCTATGGAGAAAGCACAGTTACCAGTTATCAGACATCGTCTGGACAGCAGTTGACTTCTAAGGTAACATATATGACAGACCACTGTTTACTGCAGTACTACATGAAAGATAGAAATATGACCATGATTTCTTGCATCATAGTTGATGAGGCTCATGAAAGGACCTTGAGTACAGATCTCCTTTTAGCATTGATCAAAGATTTACTGGGTCGAAGGCATCAGTTAAGGCTTATAATAATGTCTGCAACAGCTGATGCAGAAGTGCTTTCACATTATTTTTTCAACTGTAAAATCTTTCATGTAGTGGGGAGGAACTTTCCAGTTGATGTAAAATATGCTCCCTCTTTCACTGACGGAACTGCCAGTAATGTTGCTTCTTATGTTTCTGATGTGATGAGGGTAGCAAGAGAGATCCACAAGAATGAGAAACAAGGGACAATTCTTGCATTTTTGACTTCTCAGATGGAAGTCGAATGGGTCTGTGAGAAGTTTATAACACCTGGTACAATTGCATTGCCATTGCATGGGAAACTTTCTTTTGAAGAACAATCTAATGTTTTTCAAAATCACCCTGGAAAAAGAAAAATTATATTCGCCACAAATCTGGCGGAGACATCCCTGACAATTCCTGGGGTAAAGTATGTGATCGATTCTGGCATGGTCAAAGAAAGCAAATTTGAACCTGGAAGTGGTATGAATGTTCTCAGAGTTTGCAGGATCAGCCAGAGTTCTGCTAAGCAAAGAACTGGGCGTGCTGGAAGAACGGAACCTGGAATATGTTATAGACTCTACTCAGAATATGATTTTCAGGCGATGCCTCCATGTCAGGAGCCCGAGATCCGTAGAGTTCATCTTGGTGTAGCAGTTCTGAGGATTCTGGCTTTGGGTGTCAAGAATTTAAAAGAGTTTGAATTCATTGATGCACCTTGTTCTGAAGCTATAGACATGGCAATGAGGAATCTTGTTCAGTTAGGAGCTGTTAAGCAAAACAATGATGTCTATGAACTAACTCAAGAGGGTCGCGACTTGGTTAAATTGGGGGTTGAGCCTAGGCTTGGTAAATTAATTCTTGGCTGCTATCACCATAACTTGCGTAAGGAGGGCCTTGTTCTTGCGGCTGTAATGGCAAATGCAAGTAGCATATTTTGCAGAGTTGGTAATGACGAGGAGAAACTTAGGTCAGACTGCTTCAAGGTGCAATTTTGCCATCGTGATGGTGACCTCTTTACCCTTCTATCAGTGTACAAGATATGGGAAGCTCTGCCTCGAGAGAGGAGAAACACATGGTGTTGGGAAAACAGCATCAATGCCAAAACTATGAGGAGATGCCAGGACACTGTATTGGAATTGGAGTCTTGCCTTAAACATGAGCTCAATAGGATAATTCCTTGTAGCTGGCGTTGGAATGCGCATGTTTCAACTGATTGTGATATATACTTGAAAAAGGTTATACTTTCTTCCCTGGCAGAAAATGTAGCAATGTTCTCAGGATATGATCAAGTTGGTTATGAGGTGGCATTAACTGGGCAGCATGTTCGGCTGCACCCTTCTTGTTCATTACTGGTATTCGGCCAGAAACCTAGTTGGGTTGTTTTTGGTGAACTCCTCTCAAGTTCTAATCAGTATTTGGCCTGCGTCACTACCATTGATTTTAACTCTTTGTCTACCCTTGATCCCCCTCCTGTGTTTGATGTGTCCAAGATGGAAGGTCGAAAGTTGCAACTGAAAGTGTTGACTGGTTTTGGCAGTTGTTTGCTTAAAAGATTTTGTGGGAAGGGCAACAGTTATCTGCTTCATCTTGTATCTCGCGTCAGGACAATTTGCAATGACGAACTTATCAGCATTAAAGTCGATTATTATCAGAATGAGATTATGGTGTTTGCTACCCCACATAACATGGATAGGGTTATAAACCTTGTATCCGATGCATTGGAGTGTGAAAAAAGATGGCTGCGTAATGAATGCTTGGAGAAATGCTTGTATCATGGTTCTGGTGGCTTGCCTCCTGTAGCATTGTTTGGGGCTGGTGCAGAGATCAAGCATCTGGAGCTTCATAAAAGGTGTTTGACTGTTGATGTATTTCATTCAAAACTGGATGACATGGATGATAAGGAGTTTCTTACTGAGCTTGAAGAGTCTGCCTCTGGTAGTATCTGTGGTCATCATAAGTTCCCCGGCACTGGGCAGGAAAATGTTGACAAAGGAAAGGGGGCCAGGTTGACATTTTTTACTCCTGATGCTGCTCAGAAAGCTGTTGAATTAAATGATTCTGAGTTTAATGGTTCTATACTGAAGGTTGTTCCTTCACAGGTTGGAGGTGATCATAAGGTCTATCCACTCCTTGCTGTTAGAGCTAAAATTTTATGGCCTCGCAGGCAAAGCCACGGGTTTGCAATTGTTAAATGTGACATGGATGATATTTACCCCATGCTTGCTGATTTTTCTAACCTTGTAGTTTTAGGAAGAAATATTCGTTGTCAGCCTAGCAAAAGATACATGGACTCTGTTGTGATTAGTGGACTAAACAGAGATCTTTCTGAGAAAGAAATCCTTGATGTGTTAAGGACTGCTACTAGTAGGACAATCCTGGATTTTTTTCTGGTGAGAGGGGATGCTGTAGAAAATCCGCCATGTGGTGCATGTGAAGAAGCACTGCTAAAGGAAATATCACCCTATATGCCTAAACGGTACTCCCATAGTAGCTGCACTGTTCAGGTTTTCCAACCTGAACCAAAGAGTCATTTCATGAGAGCTTTGATAACATTTGACGGAAGATTACACTTGGAGGCAGCAAAGGCTTTAGAACAATTGGAAGGGAAAGTATTGCCCGGCTTCCTTCCATGGCAAAAGATGGAGTGTCAGCAGTTATTTCATAGCTCTCTGTCCTGCCCTGGCCCTGTTTATCGTGTAATAAAGAAGCAGCTAGATCCTTTACTTGAAAGTTTTACGCATCTAAAAGGTGTAGAATGTAATCTCGAGGAGTATCCTAATGGTTCCTGCAGAGTTAAGATATCAGCCAATGCTACAAAAACGATTGCTGATCTGAGGAGACGTGTGGAGGAGCTTGTGAGGGGGAAGACTATAGACCACCCAAGCCTCACTGCAACTGTTTTGCAGCTTCTGTTTTCCAGAGATGGCACCAGTCTAATGAACTCGTTACAGCGAGAGACAGGAACATATATCATTTTTGACAGGCACAAACTCAATGTACAGGTCTTTGGTGAAGCACATAAAGTTGACATGGTCAAGCAGAAATTGGTTGAATCCCTTTTAAACCTCCATGAAAGCAAGGTGCTTGAGATCCGCCTTCAAGGTAATGCTTTACCTCCTGAGTTGATGAAAGAGGTTGTTAGAAGGTTTGGGCCAGACCTCCGAGGGCTCAAAGAGAAGGTACCAGGGGCTGATTTTACTCTAAATGTACGCCGTCAATCCATTCTCATCCACGGAAGCAAGGAGTTGAAGCAAAAAGTTGAAGAGATTATTGATGAGACTGCACATATGGCTGGTAGTTTGACTAAGAGGATAAATAGGGAAGCTGATTGTCCAATCTGCTTGTGTGATGTTGAAGACGGTTACCGGCTTGAGGACTGTGGTCACTTGTTTTGTCGTTCATGTTTGGTGGAGCAGTGCGAGTCTGCAATCAATAATCATGATAGCTTTCCATTATGCTGCACTCATGAGGGTTGCAGGTCTCCAATTTTGATCACAGATCTCAGATCTCTTTTATCAATTGAGAAGCTGGAAGATCTGTTTAGGGCTTCGGTGGGGTCATATGTTGCTTCAAGTTGTGGCACCTACAGGTTTTGCCCATCTCCCGACTGTGCTTCAGTCTATCAGGTAGCGGCTCCTGGAACAGAAGGTGAACCATTTGTTTGTGGTGCATGTTATGCGGAGACGTGTACCATGTGCCATTTAGAACATCATCCTTACATGTCATGCAAGCAGTACAAGAATTTCAAGGAAGATCCGGATTCTTCATTGAAGGAGTGGTGCAAAGGAAAAGAACATGTGAAGAGCTGCCCGGTTTGCAGATATACAATTGAGAAGATAGATGGGTGCAACCATATAGAATGTCGGTGCGGGAAGCATATTTGCTGGGTTTGTTTGGCCTATTATGGAAGCAGCGATGAATGTTATGGCCACTTGAGAAGTGTTCACCTGGCAATCATTTGA

Protein Analysis

1715

Amino Acids

194.42

Weight (kDa)

7.98

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 258 - 412 2.9e-10 DEAD/DEAH box helicase
AAA_19 PF13245 260 - 402 3.9e-06 AAA domain
AAA_22 PF13401 268 - 405 5.4e-06 AAA domain
Helicase_C PF00271 460 - 580 1.2e-14 Helicase conserved C-terminal domain
OB_NTP_bind PF07717 805 - 879 5.4e-10 Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11 PF24475 981 - 1095 2.9e-48 DEAH11-like, RNA-binding domain
RRM_DEAH11 PF24637 1115 - 1195 1.8e-36 DEAH11 RRM domain
KH_DEAH11_1st PF24638 1284 - 1348 4e-31 DEAH11 eukaryotic type KH-domain
KH_DEAH11_2nd PF24641 1349 - 1422 8e-36 DEAH11 eukaryotic type KH-domain
KH_DEAH11 PF24471 1424 - 1491 9e-30 DEAH11-like, KH-domain
IBR PF01485 1582 - 1638 3.6e-07 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1208, 1469, 2885
Acc16I TGCGCA 1 cut(s) 2385
Acc36I ACCTGC 2 cut(s) 799, 4653
Acc65I GGTACC 1 cut(s) 4366
AccB1I GGYRCC 3 cut(s) 4112, 4366, 4766
AccB7I CCANNNNNTGG 2 cut(s) 472, 1499
AccI GTMKAC 1 cut(s) 2639
AccII CGCG 8 cut(s) 72, 85, 239, 254, 269, 301, 1998, 2784
AccIII TCCGGA 2 cut(s) 220, 4942
AclWI GGATC 9 cut(s) 16, 596, 1381, 1700, 1807, 2642, 3896, 4270, 4934
AcoI YGGCCR 9 cut(s) 18, 89, 110, 158, 409, 474, 504, 2545, 5113
AcuI CTGAAG 3 cut(s) 1929, 3287, 4785
AcyI GRCGYC 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 2262
AflIII ACRYGT 4 cut(s) 4027, 4871, 4905, 4981
AhlI ACTAGT 1 cut(s) 3548
AjiI CACGTC 2 cut(s) 4028, 4872
Alw21I GWGCWC 1 cut(s) 2349
Alw26I GTCTC 8 cut(s) 358, 824, 1580, 4018, 4136, 4457, 4671, 4862
AlwI GGATC 9 cut(s) 16, 596, 1381, 1700, 1807, 2642, 3896, 4270, 4934
AlwNI CAGNNNCTG 2 cut(s) 4096, 4376
Ama87I CYCGRG 3 cut(s) 1808, 2241, 3950
Aor13HI TCCGGA 2 cut(s) 220, 4942
ArsI GACNNNNNNTTYG 2 cut(s) 267, 299
AseI ATTAAT 1 cut(s) 2039
Asp718I GGTACC 1 cut(s) 4366
AspA2I CCTAGG 1 cut(s) 2024
AspLEI GCGC 5 cut(s) 74, 256, 271, 465, 2386
AspS9I GGNCC 8 cut(s) 60, 148, 385, 1137, 2077, 3186, 3869, 4337
AsuC2I CCSGG 5 cut(s) 212, 311, 3150, 3804, 4997
AvaI CYCGRG 3 cut(s) 1808, 2241, 3950
AvaII GGWCC 4 cut(s) 60, 148, 385, 1137
AvrII CCTAGG 1 cut(s) 2024
BalI TGGCCA 3 cut(s) 476, 506, 5115
BanI GGYRCC 3 cut(s) 4112, 4366, 4766
BanII GRGCYC 3 cut(s) 1809, 2349, 4356
BarI GAAGNNNNNNTAC 2 cut(s) 1333, 1365
BbsI GAAGAC 2 cut(s) 4058, 4548
Bbv12I GWGCWC 1 cut(s) 2349
BceAI ACGGC 6 cut(s) 62, 76, 104, 346, 448, 4383
BcgI CGANNNNNNTGC 4 cut(s) 2492, 2526, 2806, 2840
BciVI GTATCC 2 cut(s) 2905, 3969
BclI TGATCA 5 cut(s) 177, 1167, 2470, 3295, 4677
BcnI CCSGG 5 cut(s) 212, 311, 3150, 3804, 4997
BcoDI GTCTC 8 cut(s) 358, 824, 1580, 4018, 4136, 4457, 4671, 4862
BcuI ACTAGT 1 cut(s) 3548
BfaI CTAG 5 cut(s) 2025, 2556, 3459, 3549, 3899
BfmI CTRYAG 7 cut(s) 1067, 1914, 2982, 3591, 3973, 4056, 4771
BfoI RGCGCY 1 cut(s) 466
BfuAI ACCTGC 2 cut(s) 799, 4653
BfuI GTATCC 2 cut(s) 2905, 3969
BglI GCCNNNNNGGC 1 cut(s) 3349
BglII AGATCT 5 cut(s) 1150, 3505, 4684, 4691, 4720
BlnI CCTAGG 1 cut(s) 2024
BlpI GCTNAGC 1 cut(s) 1710
BmcAI AGTACT 1 cut(s) 1073
Bme18I GGWCC 4 cut(s) 60, 148, 385, 1137
BmeT110I CYCGRG 3 cut(s) 1808, 2241, 3950
BmgBI CACGTC 2 cut(s) 4028, 4872
BmgT120I GGNCC 8 cut(s) 60, 148, 385, 1137, 2077, 3186, 3869, 4337
BmrI ACTGGG 4 cut(s) 1190, 1731, 2508, 3165
BmuI ACTGGG 4 cut(s) 1190, 1731, 2508, 3165
BoxI GACNNNNGTC 1 cut(s) 4566
BpiI GAAGAC 2 cut(s) 4058, 4548
BplI GAGNNNNNCTC 2 cut(s) 3101, 3133
BpmI CTGGAG 2 cut(s) 401, 3041
Bpu1102I GCTNAGC 1 cut(s) 1710
BpuEI CTTGAG 7 cut(s) 809, 1162, 1971, 2569, 4292, 4577, 5140
BpuMI CCSGG 5 cut(s) 212, 311, 3150, 3804, 4997
Bsa29I ATCGAT 1 cut(s) 1623
BsaBI GATNNNNATC 1 cut(s) 2832
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 4671
BsaWI WCCGGW 3 cut(s) 220, 538, 4942
BsaXI ACNNNNNCTCC 6 cut(s) 135, 165, 338, 368, 539, 569
Bse118I RCCGGY 3 cut(s) 16, 407, 4551
Bse3DI GCAATG 5 cut(s) 1487, 1493, 1932, 2460, 2806
Bse8I GATNNNNATC 1 cut(s) 2832
BseAI TCCGGA 2 cut(s) 220, 4942
BseCI ATCGAT 1 cut(s) 1623
BseGI GGATG 7 cut(s) 1589, 3076, 3085, 3394, 3592, 4413, 4897
BseJI GATNNNNATC 1 cut(s) 2832
BseMI GCAATG 5 cut(s) 1487, 1493, 1932, 2460, 2806
BseRI GAGGAG 8 cut(s) 665, 2156, 2263, 2311, 2570, 3968, 4036, 4049
BseX3I CGGCCG 5 cut(s) 18, 89, 110, 158, 409
BseYI CCCAGC 1 cut(s) 5070
BsgI GTGCAG 7 cut(s) 208, 577, 2501, 3024, 3664, 4452, 4630
Bsh1236I CGCG 8 cut(s) 72, 85, 239, 254, 269, 301, 1998, 2784
Bsh1285I CGRYCG 7 cut(s) 21, 92, 113, 161, 310, 412, 490
BshNI GGYRCC 3 cut(s) 4112, 4366, 4766
BshVI ATCGAT 1 cut(s) 1623
BsiEI CGRYCG 7 cut(s) 21, 92, 113, 161, 310, 412, 490
BsiHKAI GWGCWC 1 cut(s) 2349
BsiHKCI CYCGRG 3 cut(s) 1808, 2241, 3950
BslFI GGGAC 3 cut(s) 46, 161, 1423
BsmAI GTCTC 8 cut(s) 358, 824, 1580, 4018, 4136, 4457, 4671, 4862
BsmBI CGTCTC 3 cut(s) 358, 4018, 4862
BsmFI GGGAC 3 cut(s) 46, 161, 1423
BsmI GAATGC 2 cut(s) 2386, 2942
Bso31I GGTCTC 1 cut(s) 4671
BsoBI CYCGRG 3 cut(s) 1808, 2241, 3950
Bsp1286I GDGCHC 3 cut(s) 1809, 2349, 4356
Bsp13I TCCGGA 2 cut(s) 220, 4942
Bsp1407I TGTACA 2 cut(s) 2217, 4183
Bsp1720I GCTNAGC 1 cut(s) 1710
Bsp19I CCATGG 1 cut(s) 3815
Bsp68I TCGCGA 1 cut(s) 1998
BspDI ATCGAT 1 cut(s) 1623
BspEI TCCGGA 2 cut(s) 220, 4942
BspFNI CGCG 8 cut(s) 72, 85, 239, 254, 269, 301, 1998, 2784
BspHI TCATGA 4 cut(s) 1129, 3720, 4624, 4651
BspMAI CTGCAG 2 cut(s) 1071, 3977
BspMI ACCTGC 2 cut(s) 799, 4653
BspPI GGATC 9 cut(s) 16, 596, 1381, 1700, 1807, 2642, 3896, 4270, 4934
BspQI GCTCTTC 1 cut(s) 4982
BspT107I GGYRCC 3 cut(s) 4112, 4366, 4766
BspTNI GGTCTC 1 cut(s) 4671
BsrDI GCAATG 5 cut(s) 1487, 1493, 1932, 2460, 2806
BsrFI RCCGGY 3 cut(s) 16, 407, 4551
BsrGI TGTACA 2 cut(s) 2217, 4183
BssAI RCCGGY 3 cut(s) 16, 407, 4551
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 3 cut(s) 701, 2024, 3815
Bst6I CTCTTC 5 cut(s) 545, 839, 3102, 4443, 4982
BstACI GRCGYC 1 cut(s) 339
BstAPI GCANNNNNTGC 2 cut(s) 2121, 4862
BstAUI TGTACA 2 cut(s) 2217, 4183
BstDSI CCRYGG 3 cut(s) 3358, 3815, 4417
BstEII GGTNACC 2 cut(s) 2192, 4547
BstF5I GGATG 7 cut(s) 1589, 3076, 3085, 3394, 3592, 4413, 4897
BstFNI CGCG 8 cut(s) 72, 85, 239, 254, 269, 301, 1998, 2784
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 5 cut(s) 74, 256, 271, 465, 2386
BstMAI GTCTC 8 cut(s) 358, 824, 1580, 4018, 4136, 4457, 4671, 4862
BstMCI CGRYCG 7 cut(s) 21, 92, 113, 161, 310, 412, 490
BstNSI RCATGY 6 cut(s) 2390, 2510, 3618, 4860, 4909, 4985
BstPAI GACNNNNGTC 1 cut(s) 4566
BstPI GGTNACC 2 cut(s) 2192, 4547
BstSFI CTRYAG 7 cut(s) 1067, 1914, 2982, 3591, 3973, 4056, 4771
BstUI CGCG 8 cut(s) 72, 85, 239, 254, 269, 301, 1998, 2784
BstV2I GAAGAC 2 cut(s) 4058, 4548
BstXI CCANNNNNNTGG 3 cut(s) 940, 1579, 2189
BstZI CGGCCG 5 cut(s) 18, 89, 110, 158, 409
Bsu15I ATCGAT 1 cut(s) 1623
BsuI GTATCC 2 cut(s) 2905, 3969
BsuTUI ATCGAT 1 cut(s) 1623
BtgI CCRYGG 3 cut(s) 3358, 3815, 4417
BtgZI GCGATG 3 cut(s) 293, 1802, 5115
BtrI CACGTC 2 cut(s) 4028, 4872
BtsCI GGATG 7 cut(s) 1589, 3076, 3085, 3394, 3592, 4413, 4897
BtsI GCAGTG 4 cut(s) 263, 3626, 4074, 4607
BtuMI TCGCGA 1 cut(s) 1998
BveI ACCTGC 2 cut(s) 799, 4653
CaiI CAGNNNCTG 2 cut(s) 4096, 4376
CciI TCATGA 4 cut(s) 1129, 3720, 4624, 4651
CfoI GCGC 5 cut(s) 74, 256, 271, 465, 2386
Cfr10I RCCGGY 3 cut(s) 16, 407, 4551
Cfr13I GGNCC 8 cut(s) 60, 148, 385, 1137, 2077, 3186, 3869, 4337
ClaI ATCGAT 1 cut(s) 1623
CseI GACGC 5 cut(s) 228, 275, 347, 2599, 2773
CsiI ACCWGGT 1 cut(s) 1474
DraI TTTAAA 3 cut(s) 682, 1875, 4248
DraIII CACNNNGTG 1 cut(s) 2262
EaeI YGGCCR 9 cut(s) 18, 89, 110, 158, 409, 474, 504, 2545, 5113
EagI CGGCCG 5 cut(s) 18, 89, 110, 158, 409
Eam1104I CTCTTC 5 cut(s) 545, 839, 3102, 4443, 4982
EarI CTCTTC 5 cut(s) 545, 839, 3102, 4443, 4982
EciI GGCGGA 3 cut(s) 1598, 3591, 4268
Ecl136II GAGCTC 1 cut(s) 2347
EclXI CGGCCG 5 cut(s) 18, 89, 110, 158, 409
Eco130I CCWWGG 3 cut(s) 701, 2024, 3815
Eco147I AGGCCT 1 cut(s) 514
Eco24I GRGCYC 3 cut(s) 1809, 2349, 4356
Eco31I GGTCTC 1 cut(s) 4671
Eco32I GATATC 1 cut(s) 3987
Eco47I GGWCC 4 cut(s) 60, 148, 385, 1137
Eco52I CGGCCG 5 cut(s) 18, 89, 110, 158, 409
Eco53kI GAGCTC 1 cut(s) 2347
Eco57I CTGAAG 3 cut(s) 1929, 3287, 4785
Eco88I CYCGRG 3 cut(s) 1808, 2241, 3950
Eco91I GGTNACC 2 cut(s) 2192, 4547
EcoICRI GAGCTC 1 cut(s) 2347
EcoO109I RGGNCCY 4 cut(s) 60, 148, 1137, 2077
EcoO65I GGTNACC 2 cut(s) 2192, 4547
EcoRI GAATTC 3 cut(s) 102, 711, 1886
EcoRV GATATC 1 cut(s) 3987
EcoT14I CCWWGG 3 cut(s) 701, 2024, 3815
EcoT22I ATGCAT 1 cut(s) 2905
EcoT38I GRGCYC 3 cut(s) 1809, 2349, 4356
ErhI CCWWGG 3 cut(s) 701, 2024, 3815
Esp3I CGTCTC 3 cut(s) 358, 4018, 4862
FalI AAGNNNNNCTT 2 cut(s) 3510, 3542
FaqI GGGAC 3 cut(s) 46, 161, 1423
FauI CCCGC 1 cut(s) 5048
FauNDI CATATG 2 cut(s) 4473, 4747
FbaI TGATCA 5 cut(s) 177, 1167, 2470, 3295, 4677
FblI GTMKAC 1 cut(s) 2639
FokI GGATG 7 cut(s) 1576, 3083, 3092, 3401, 3599, 4400, 4884
FriOI GRGCYC 3 cut(s) 1809, 2349, 4356
FspAI RTGCGCAY 1 cut(s) 2385
FspBI CTAG 5 cut(s) 2025, 2556, 3459, 3549, 3899
FspI TGCGCA 1 cut(s) 2385
GlaI GCGC 5 cut(s) 73, 255, 270, 464, 2385
GsaI CCCAGC 1 cut(s) 5074
GsuI CTGGAG 2 cut(s) 401, 3041
HaeII RGCGCY 1 cut(s) 466
HgaI GACGC 5 cut(s) 228, 275, 347, 2599, 2773
HhaI GCGC 5 cut(s) 74, 256, 271, 465, 2386
Hin1I GRCGYC 1 cut(s) 339
Hin6I GCGC 5 cut(s) 72, 254, 269, 463, 2384
HinP1I GCGC 5 cut(s) 72, 254, 269, 463, 2384
HincII GTYRAC 5 cut(s) 1029, 2709, 3172, 3195, 4213
HindII GTYRAC 5 cut(s) 1029, 2709, 3172, 3195, 4213
HindIII AAGCTT 1 cut(s) 332
Hpy99I CGWCG 6 cut(s) 42, 90, 244, 269, 434, 440
HpyCH4IV ACGT 2 cut(s) 4027, 4871
HpySE526I ACGT 2 cut(s) 4027, 4871
Hsp92I GRCGYC 1 cut(s) 339
HspAI GCGC 5 cut(s) 72, 254, 269, 463, 2384
KflI GGGWCCC 1 cut(s) 60
Kpn2I TCCGGA 2 cut(s) 220, 4942
KpnI GGTACC 1 cut(s) 4370
KroI GCCGGC 2 cut(s) 16, 407
KroNI GCCGGC 2 cut(s) 18, 409
Ksp22I TGATCA 5 cut(s) 177, 1167, 2470, 3295, 4677
LguI GCTCTTC 1 cut(s) 4982
LmnI GCTCC 8 cut(s) 376, 1318, 1804, 1949, 3022, 4036, 4597, 4825
MabI ACCWGGT 1 cut(s) 1474
MaeI CTAG 5 cut(s) 2025, 2556, 3459, 3549, 3899
MaeII ACGT 2 cut(s) 4027, 4871
MaeIII GTNAC 8 cut(s) 994, 1039, 2192, 2611, 3380, 4131, 4547, 4568
MfeI CAATTG 6 cut(s) 854, 1482, 3369, 3782, 4704, 5013
MhlI GDGCHC 3 cut(s) 1809, 2349, 4356
MlsI TGGCCA 3 cut(s) 476, 506, 5115
MluNI TGGCCA 3 cut(s) 476, 506, 5115
MmeI TCCRAC 5 cut(s) 162, 627, 2357, 3268, 3721
Mox20I TGGCCA 3 cut(s) 476, 506, 5115
Mph1103I ATGCAT 1 cut(s) 2905
MroI TCCGGA 2 cut(s) 220, 4942
MroNI GCCGGC 2 cut(s) 16, 407
MscI TGGCCA 3 cut(s) 476, 506, 5115
MslI CAYNNNNRTG 5 cut(s) 1395, 1830, 1925, 2187, 2908
Msp20I TGGCCA 3 cut(s) 476, 506, 5115
MspA1I CMGCKG 2 cut(s) 109, 1226
MunI CAATTG 6 cut(s) 854, 1482, 3369, 3782, 4704, 5013
Mva1269I GAATGC 2 cut(s) 2386, 2942
MvnI CGCG 8 cut(s) 72, 85, 239, 254, 269, 301, 1998, 2784
NaeI GCCGGC 2 cut(s) 18, 409
NciI CCSGG 5 cut(s) 212, 311, 3150, 3804, 4997
NcoI CCATGG 1 cut(s) 3815
NdeI CATATG 2 cut(s) 4473, 4747
NgoMIV GCCGGC 2 cut(s) 16, 407
NmuCI GTSAC 4 cut(s) 2192, 2611, 3380, 4568
NruI TCGCGA 1 cut(s) 1998
NsbI TGCGCA 1 cut(s) 2385
NsiI ATGCAT 1 cut(s) 2905
NspI RCATGY 6 cut(s) 2390, 2510, 3618, 4860, 4909, 4985
PaeR7I CTCGAG 2 cut(s) 2241, 3950
PagI TCATGA 4 cut(s) 1129, 3720, 4624, 4651
PceI AGGCCT 1 cut(s) 514
PciI ACATGT 2 cut(s) 4905, 4981
PciSI GCTCTTC 1 cut(s) 4982
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 435
PctI GAATGC 2 cut(s) 2386, 2942
PdiI GCCGGC 2 cut(s) 18, 409
PfeI GAWTC 8 cut(s) 875, 882, 1625, 1849, 1933, 3242, 4238, 4946
PflFI GACNNNGTC 3 cut(s) 350, 1012, 4217
PflMI CCANNNNNTGG 2 cut(s) 472, 1499
PfoI TCCNGGA 2 cut(s) 3561, 4822
PpuMI RGGWCCY 3 cut(s) 60, 148, 1137
PscI ACATGT 2 cut(s) 4905, 4981
PshAI GACNNNNGTC 1 cut(s) 4566
PshBI ATTAAT 1 cut(s) 2039
PsiI TTATAA 3 cut(s) 1208, 1469, 2885
Psp124BI GAGCTC 1 cut(s) 2349
Psp5II RGGWCCY 3 cut(s) 60, 148, 1137
PspEI GGTNACC 2 cut(s) 2192, 4547
PspFI CCCAGC 1 cut(s) 5070
PspPI GGNCC 8 cut(s) 60, 148, 385, 1137, 2077, 3186, 3869, 4337
PspPPI RGGWCCY 3 cut(s) 60, 148, 1137
PstI CTGCAG 2 cut(s) 1071, 3977
PstNI CAGNNNCTG 2 cut(s) 4096, 4376
PsyI GACNNNGTC 3 cut(s) 350, 1012, 4217
PvuII CAGCTG 1 cut(s) 1226
RruI TCGCGA 1 cut(s) 1998
RseI CAYNNNNRTG 5 cut(s) 1395, 1830, 1925, 2187, 2908
SacI GAGCTC 1 cut(s) 2349
SapI GCTCTTC 1 cut(s) 4982
Sau96I GGNCC 8 cut(s) 60, 148, 385, 1137, 2077, 3186, 3869, 4337
ScaI AGTACT 1 cut(s) 1073
SduI GDGCHC 3 cut(s) 1809, 2349, 4356
SexAI ACCWGGT 1 cut(s) 1474
SfcI CTRYAG 7 cut(s) 1067, 1914, 2982, 3591, 3973, 4056, 4771
Sfr274I CTCGAG 2 cut(s) 2241, 3950
SinI GGWCC 4 cut(s) 60, 148, 385, 1137
SlaI CTCGAG 2 cut(s) 2241, 3950
SmiMI CAYNNNNRTG 5 cut(s) 1395, 1830, 1925, 2187, 2908
SmlI CTYRAG 9 cut(s) 788, 1141, 1986, 2241, 2584, 3950, 4271, 4556, 5119
SmoI CTYRAG 9 cut(s) 788, 1141, 1986, 2241, 2584, 3950, 4271, 4556, 5119
SpeI ACTAGT 1 cut(s) 3548
SseBI AGGCCT 1 cut(s) 514
SspI AATATT 1 cut(s) 3445
SspMI CTAG 5 cut(s) 2025, 2556, 3459, 3549, 3899
SstI GAGCTC 1 cut(s) 2349
StuI AGGCCT 1 cut(s) 514
StyI CCWWGG 3 cut(s) 701, 2024, 3815
TaiI ACGT 2 cut(s) 4030, 4874
TaqII GACCGA 1 cut(s) 504
TatI WGTACW 6 cut(s) 848, 1071, 1145, 2217, 4183, 4920
TauI GCSGC 6 cut(s) 77, 112, 160, 304, 2093, 4820
TfiI GAWTC 8 cut(s) 875, 882, 1625, 1849, 1933, 3242, 4238, 4946
TseFI GTSAC 4 cut(s) 2192, 2611, 3380, 4568
Tsp45I GTSAC 4 cut(s) 2192, 2611, 3380, 4568
TspGWI ACGGA 6 cut(s) 340, 1344, 1754, 1805, 3759, 4434
Tth111I GACNNNGTC 3 cut(s) 350, 1012, 4217
Van91I CCANNNNNTGG 2 cut(s) 472, 1499
VpaK11BI GGWCC 4 cut(s) 60, 148, 385, 1137
VspI ATTAAT 1 cut(s) 2039
XceI RCATGY 6 cut(s) 2390, 2510, 3618, 4860, 4909, 4985
XhoI CTCGAG 2 cut(s) 2241, 3950
XmaJI CCTAGG 1 cut(s) 2024
XmiI GTMKAC 1 cut(s) 2639
XspI CTAG 5 cut(s) 2025, 2556, 3459, 3549, 3899
ZrmI AGTACT 1 cut(s) 1073
Zsp2I ATGCAT 1 cut(s) 2905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.