Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Reverse (-)

32802686 .. 32806952

Gene Structure 4267 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1318100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3294 bp

ATGAGATCATCATACCCGCGCACTACTTCCCGGCAACCTCAGCCGTCGGGAATTCCCCGCCGTTTGCCTCCCCGGCGACCCAGTTACCCGCCCAACAACTTCTACCGCCAAACCCCTCCTCCCTCCCGCACAAGTTTCATCGTCCAGCTACTCGCCGATCACCGGAGTCTCCGAAAGCATGAAATCGACGCCGTGATCGCTAACTGCAAGGCCAGGCCCGAACGCGTTGAGGTTTCTCCAGCCAGTCGCATCGTGGCCTCCTTGTTCTACACGCAATGGGTCGATGCGCTAGAAGCCCTCGTTTATTTTTGGGAGTCCCGACTGGACCGGTTTCATGATCTCACTCCCAAGTTGAACCGAATTGTCTCTGTTGCCTCCGATCTCGACGAGCTCCGGGACCGGCTGAAAGCCCTGTTCTCCGGCCGGATTAAGAAATTGATGGACGGAGAGGCGGTGAAGAAGTGGGAGGCGAAGCGAGTTTGGCTGTGCAACGACAACGAACGAGTTTCCAAGTTGCTCCGGCGGCATAACCATCTGTGGAGTTTCAGTGAGTTGAATGAGACACTAAAGAGGAACAGGAGCGAAATTCAATTGGTTGAGAGCAGGATTAGGGAGTTTAAGTCCGCCATGAATTGCTTGCTTGATTATCTTGAGGGGAAAAGGGCGGAGGAATCGGGTGAAGAAGGGGTGAAAGTGTTCAAATTTTCGAGTGAAGTTTATGATTGGAGTCAAATTCACAGCTTAATAGTTCGGGAATGCCGCAGGCTCGAAGAAGGGTTGCCCATATACGCTTATCGGCAGCAAATTCTACAGCAAATTCTTACCCAGCAGGTCACGGTATTGATTGGAGAGACTGGCTCAGGGAAAAGTACACAGCTGGTGCAGTTTCTTGTCGATTCTGGAACTGCTGCCGAGCAGTCCATTGTATGCACTCAGCCTCGAAAGATTGCTGCCATGTCGTTGTCGCAGAGAGTTAGAGAAGAAAGTAATGGGTGTTACAGAGGAAAATCAGTATCTTGTAATTCAACATTTTTGTCAGGGGAGCAGTTGGTTTCTAAGGTGACATTTATGACAGATCACTATTTACTGCAGCATTACATGAATGATACTAAGATGTCTGGTATTTCTTGCATAATAGTCGATGAGGCTCACGAAAGGAGCCTGAATACTGATCTCCTGTTGGCATTGTTAAAAGATTTACTTGGTCGACGGTCTAGTTTACGACTTATTATAATGTCTGCAACAGCTGATGCCAAAGTGCTTTCCGAATATTTTTATCATTGTGGAATCTTTTATGTATTGGGGAGGAGTTTTCGCGTTGATGTTAGATATGTACCATCTTTCACTGAAGGAACTTCTAGTGATGCTTCCTATGTTTCTGATGTGATGAGGGTAGCAACAGATGTACACAGAAAACAGAAAGAGGGGACGATTCTTGCCTTTTTGACTTCCCAGTTGGAAGTAGAATGGTGTTGTGATAAGTTTATAGCATCAGGTGCAATTGCATTGCCCTTGCATGGAAAACTCTCTTATGAAGATCAATTTAATGTTTTCCAAAACTACCCTGGAAAGAGGAAAATCATATTCGCCACTAATCTGGCTGAGACATCTCTGACAATTCCTGGGGTAAAGTATGTTATTGATTCTGGTATGGCAAAAGAAAGCAAATTTGAACCGGGAAGTGGCATGAATGTTCTCAGAGTTTGTATGATCAGCCAGAGTTCTGCTAATCAAAGAAGTGGCCGTGCTGGAAGAACTGAACCAGGAGTATGTTATAGACTTTACTCAGAATATGACTTTGAGGCTATGCCTCCTTGTCAGGAACCTGAGATTCGCAGAGTTCATCTTGGTGTTGCAGTTCTGAGGATTCTGGCTTTAGGCGTCAAGAATTTACAAGACTTTAAATTTATTGATGCACCTTCTTCTGAAGCTATTGACTTGGCTATCAGGAACCTTGTTCAGTTGGGAGCTGTTAAGCAAAAGAATGGTGTTTTTGAGTTAACCCAGGAAGGTCGGTGCTTGGTTAATTTGGGTGTTGAGCCTCGGCTTGGTAAATTAATTCTTGGTTGTTGTGACCTTAACTTGCGTATGGAGGGTCTTGTTCTTGCAGCTGTAATGGCAAATTCCCGCAGCATATTTTGTAGAGTTGGTAATGATGAAGAAAAGCTTCGATCAGACTGCATCAAGGTGAAATTTTGCCATCGTGATGGTGACCTCTTTACCCTTCTCTCTGTGTACAAGGAATGGGATTCTTTGTCTCGAGACAAGAGAAATGTGTGGTGCTGGGAAAACAGCATCAATGCCAAAACAATGAGGAGATGCCAAGACACGGTGATGGAATTGGAGTCTTGCCTTAAACATGAACTCCACATGATAATTTCTGGTACCTGGCGTTGGGATCCACGTGAATCAAATGATTGTGACAAACATTTGAAAAAGGTTATACTTTCTTCCCTTGCAGATAATGTTGCTATGTTCTCAGGTTATGATCAACTTGGTTATGAAGTGGCATTAACTGGGCAGCATGTTAAGCTGCATCCTTCGTGTTCATTGCTAGTATTTGGGGAGAAACCTAGTTGGGTAGTGTTTGGTGAAATCCTGTCGGTTTCTAATCAGTATCTGGTGTGTGTCACTTCCTTTGATTTCAACTCTTTGTCTGCGCTTTTTCCTCCTCCTTCATTGGATGTATCTAAGATGGAAAGTCGAAAGCTTCAGCTGAAAGTGTTGACTGGTTTTGGCATTACTGTTCTTAAAAGATTTTGTGGGAAGGCAAACAGTTATCTGCTTCATCTCGTGTCCCGTATTAGGACAATTTGCGAGGATGAACTTATCAACATTAAAGTCGATTACTATCAGAATGAGATCATGTTGTTTGCCACCTCAAACAACATGGATAGGGTTGAAAGCTTTGTAAATAATGCCTTGGAGTGTGAACAAAAATGGATGCGTAATGAATGCTTGGAGAAATGCTTATATCACAGTTCTGGCATCTTGCCTCCTGTAGCATTGTTTGGAGCCGGTGCAGAGATCAAACATCTGGAGCTTCAAAAAAGGTGTTTGACATTTAATGTAATTCATTCAAAACTGGATTCTGAAGATGATAAGGAGTTACTTAGTGAGCTTGAGAAGTCTATCTCTGGTGGTATATGTGCTATTCACAAGTTCCCCAGCACTGGGCAGGAAAATGTTGACAAAGGAAAATGGGTGAGGGTAACATTTCAAACTCCTGAAGCGGCTCAAAAAGCAGCTGAGCTAAACGAATCTGAGTTTAGCGGCTCCATACTGAAGAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1098

Amino Acids

124.52

Weight (kDa)

8.46

Isoelectric Point (pI)

47.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	265 - 419	1.6e-10	DEAD/DEAH box helicase
Helicase_C	PF00271	468 - 586	3.1e-14	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	811 - 887	2.8e-09	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	987 - 1096	1.2e-44	DEAH11-like, RNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1232
Acc36I	ACCTGC	1 cut(s)	820
Acc65I	GGTACC	1 cut(s)	2384
AccB1I	GGYRCC	1 cut(s)	2384
AccI	GTMKAC	1 cut(s)	1207
AccII	CGCG	3 cut(s)	19, 225, 1317
AclWI	GGATC	2 cut(s)	2393, 2406
AcoI	YGGCCR	2 cut(s)	421, 1741
AcuI	CTGAAG	5 cut(s)	1368, 1947, 2696, 3114, 3249
AcvI	CACGTG	1 cut(s)	2405
AcyI	GRCGYC	2 cut(s)	189, 1881
AfaI	GTAC	5 cut(s)	871, 1335, 1407, 2237, 2386
AfiI	CCNNNNNNNGG	9 cut(s)	162, 1517, 1682, 2048, 2329, 2394, 2805, 3173, 3174
AflIII	ACRYGT	1 cut(s)	223
AgeI	ACCGGT	1 cut(s)	327
AjnI	CCWGG	6 cut(s)	212, 1564, 1621, 1762, 2004, 2387
Alw21I	GWGCWC	1 cut(s)	393
Alw26I	GTCTC	7 cut(s)	173, 370, 554, 845, 1598, 2256, 2262
AlwI	GGATC	2 cut(s)	2393, 2406
AlwNI	CAGNNNCTG	1 cut(s)	2620
Ama87I	CYCGRG	1 cut(s)	2259
AoxI	GGCC	5 cut(s)	210, 215, 255, 421, 1741
ApeKI	GCWGC	9 cut(s)	799, 908, 950, 1090, 2108, 2130, 2521, 2533, 3245
AseI	ATTAAT	1 cut(s)	2057
AsiGI	ACCGGT	1 cut(s)	327
Asp700I	GAANNNNTTC	2 cut(s)	695, 2166
Asp718I	GGTACC	1 cut(s)	2384
AspLEI	GCGC	3 cut(s)	21, 289, 2662
AspS9I	GGNCC	3 cut(s)	216, 325, 397
AsuC2I	CCSGG	4 cut(s)	31, 73, 395, 1677
AvaI	CYCGRG	1 cut(s)	2259
AvaII	GGWCC	2 cut(s)	325, 397
BamHI	GGATCC	1 cut(s)	2398
BanI	GGYRCC	1 cut(s)	2384
BanII	GRGCYC	1 cut(s)	393
BauI	CACGAG	1 cut(s)	2792
BbrPI	CACGTG	1 cut(s)	2405
Bbv12I	GWGCWC	1 cut(s)	393
BbvCI	CCTCAGC	1 cut(s)	39
BbvI	GCAGC	9 cut(s)	811, 895, 937, 1102, 2120, 2142, 2520, 2533, 3257
BccI	CCATC	7 cut(s)	433, 540, 1345, 2201, 2208, 2329, 2689
BceAI	ACGGC	4 cut(s)	28, 45, 176, 1728
BcgI	CGANNNNNNTGC	4 cut(s)	1120, 1154, 2160, 2194
BciT130I	CCWGG	6 cut(s)	214, 1566, 1623, 1764, 2006, 2389
BclI	TGATCA	2 cut(s)	1710, 2488
BcnI	CCSGG	4 cut(s)	31, 73, 395, 1677
BcoDI	GTCTC	7 cut(s)	173, 370, 554, 845, 1598, 2256, 2262
BfaI	CTAG	5 cut(s)	290, 1215, 1359, 2555, 2574
BfmI	CTRYAG	3 cut(s)	809, 1088, 3000
BfuAI	ACCTGC	1 cut(s)	820
BglI	GCCNNNNNGGC	1 cut(s)	73
BlpI	GCTNAGC	1 cut(s)	3249
Bme18I	GGWCC	2 cut(s)	325, 397
BmeT110I	CYCGRG	1 cut(s)	2259
BmgT120I	GGNCC	3 cut(s)	216, 325, 397
BmiI	GGNNCC	8 cut(s)	398, 1160, 1824, 1952, 2386, 2400, 3016, 3277
BmrI	ACTGGG	4 cut(s)	75, 1447, 2526, 3183
BmuI	ACTGGG	4 cut(s)	75, 1447, 2526, 3183
BplI	GAGNNNNNCTC	2 cut(s)	842, 874
BpmI	CTGGAG	2 cut(s)	222, 3059
Bpu10I	CCTNAGC	2 cut(s)	39, 859
Bpu1102I	GCTNAGC	1 cut(s)	3249
BpuEI	CTTGAG	2 cut(s)	671, 3143
BpuMI	CCSGG	4 cut(s)	31, 73, 395, 1677
BsaAI	YACGTR	1 cut(s)	2405
BsaBI	GATNNNNATC	1 cut(s)	2850
BsaHI	GRCGYC	2 cut(s)	189, 1881
BsaJI	CCNNGG	6 cut(s)	71, 1564, 1622, 2004, 2042, 2922
BsaWI	WCCGGW	2 cut(s)	162, 327
Bsc4I	CCNNNNNNNGG	9 cut(s)	162, 1517, 1682, 2048, 2329, 2394, 2805, 3173, 3174
Bse118I	RCCGGY	3 cut(s)	327, 399, 3017
Bse1I	ACTGG	9 cut(s)	81, 243, 327, 859, 1453, 2521, 2734, 3090, 3178
Bse3DI	GCAATG	3 cut(s)	281, 1505, 2549
Bse8I	GATNNNNATC	1 cut(s)	2850
BseBI	CCWGG	6 cut(s)	214, 1566, 1623, 1764, 2006, 2389
BseDI	CCNNGG	6 cut(s)	71, 1564, 1622, 2004, 2042, 2922
BseGI	GGATG	4 cut(s)	2536, 2689, 2827, 2949
BseJI	GATNNNNATC	1 cut(s)	2850
BseLI	CCNNNNNNNGG	9 cut(s)	162, 1517, 1682, 2048, 2329, 2394, 2805, 3173, 3174
BseMI	GCAATG	3 cut(s)	281, 1505, 2549
BseNI	ACTGG	9 cut(s)	81, 243, 327, 859, 1453, 2521, 2734, 3090, 3178
BseRI	GAGGAG	4 cut(s)	108, 1321, 2329, 2661
BseX3I	CGGCCG	1 cut(s)	421
BseXI	GCAGC	9 cut(s)	811, 895, 937, 1102, 2120, 2142, 2520, 2533, 3257
BseYI	CCCAGC	3 cut(s)	825, 2283, 3167
BsgI	GTGCAG	2 cut(s)	902, 3042
Bsh1236I	CGCG	3 cut(s)	19, 225, 1317
Bsh1285I	CGRYCG	1 cut(s)	424
BshFI	GGCC	5 cut(s)	212, 217, 257, 423, 1743
BshNI	GGYRCC	1 cut(s)	2384
BshTI	ACCGGT	1 cut(s)	327
BsiEI	CGRYCG	1 cut(s)	424
BsiHKAI	GWGCWC	1 cut(s)	393
BsiHKCI	CYCGRG	1 cut(s)	2259
BslFI	GGGAC	4 cut(s)	301, 410, 1441, 2782
BslI	CCNNNNNNNGG	9 cut(s)	162, 1517, 1682, 2048, 2329, 2394, 2805, 3173, 3174
BsmAI	GTCTC	7 cut(s)	173, 370, 554, 845, 1598, 2256, 2262
BsmFI	GGGAC	4 cut(s)	301, 410, 1441, 2782
BsmI	GAATGC	2 cut(s)	761, 2960
BsnI	GGCC	5 cut(s)	212, 217, 257, 423, 1743
BsoBI	CYCGRG	1 cut(s)	2259
Bsp1286I	GDGCHC	1 cut(s)	393
Bsp1407I	TGTACA	2 cut(s)	1405, 2235
Bsp1720I	GCTNAGC	1 cut(s)	3249
BspANI	GGCC	5 cut(s)	212, 217, 257, 423, 1743
BspFNI	CGCG	3 cut(s)	19, 225, 1317
BspHI	TCATGA	1 cut(s)	334
BspLI	GGNNCC	8 cut(s)	398, 1160, 1824, 1952, 2386, 2400, 3016, 3277
BspMAI	CTGCAG	1 cut(s)	1092
BspMI	ACCTGC	1 cut(s)	820
BspPI	GGATC	2 cut(s)	2393, 2406
BspQI	GCTCTTC	1 cut(s)	3281
BspT107I	GGYRCC	1 cut(s)	2384
BsrDI	GCAATG	3 cut(s)	281, 1505, 2549
BsrFI	RCCGGY	3 cut(s)	327, 399, 3017
BsrGI	TGTACA	2 cut(s)	1405, 2235
BsrI	ACTGG	9 cut(s)	81, 243, 327, 859, 1453, 2521, 2734, 3090, 3178
BssAI	RCCGGY	3 cut(s)	327, 399, 3017
BssECI	CCNNGG	6 cut(s)	71, 1564, 1622, 2004, 2042, 2922
BssNI	GRCGYC	2 cut(s)	189, 1881
BssSI	CACGAG	1 cut(s)	2792
BssT1I	CCWWGG	1 cut(s)	2922
Bst2BI	CACGAG	1 cut(s)	2792
Bst2UI	CCWGG	6 cut(s)	214, 1566, 1623, 1764, 2006, 2389
Bst4CI	ACNGT	6 cut(s)	838, 1212, 2332, 2746, 2777, 2981
Bst6I	CTCTTC	1 cut(s)	3281
BstACI	GRCGYC	2 cut(s)	189, 1881
BstAPI	GCANNNNNTGC	1 cut(s)	1496
BstAUI	TGTACA	2 cut(s)	1405, 2235
BstBAI	YACGTR	1 cut(s)	2405
BstC8I	GCNNGC	2 cut(s)	638, 764
BstEII	GGTNACC	1 cut(s)	2210
BstF5I	GGATG	4 cut(s)	2536, 2689, 2827, 2949
BstFNI	CGCG	3 cut(s)	19, 225, 1317
BstHHI	GCGC	3 cut(s)	21, 289, 2662
BstMAI	GTCTC	7 cut(s)	173, 370, 554, 845, 1598, 2256, 2262
BstMCI	CGRYCG	1 cut(s)	424
BstNI	CCWGG	6 cut(s)	214, 1566, 1623, 1764, 2006, 2389
BstNSI	RCATGY	1 cut(s)	2528
BstPI	GGTNACC	1 cut(s)	2210
BstSFI	CTRYAG	3 cut(s)	809, 1088, 3000
BstUI	CGCG	3 cut(s)	19, 225, 1317
BstV1I	GCAGC	9 cut(s)	811, 895, 937, 1102, 2120, 2142, 2520, 2533, 3257
BstX2I	RGATCY	1 cut(s)	2398
BstXI	CCANNNNNNTGG	2 cut(s)	1597, 2207
BstYI	RGATCY	1 cut(s)	2398
BstZI	CGGCCG	1 cut(s)	421
BsuRI	GGCC	5 cut(s)	212, 217, 257, 423, 1743
BtsCI	GGATG	4 cut(s)	2536, 2689, 2827, 2949
BtsIMutI	CAGTG	3 cut(s)	553, 1344, 3171
BveI	ACCTGC	1 cut(s)	820
Cac8I	GCNNGC	2 cut(s)	638, 764
CaiI	CAGNNNCTG	1 cut(s)	2620
CciI	TCATGA	1 cut(s)	334
CfoI	GCGC	3 cut(s)	21, 289, 2662
Cfr10I	RCCGGY	3 cut(s)	327, 399, 3017
Cfr13I	GGNCC	3 cut(s)	216, 325, 397
CseI	GACGC	2 cut(s)	197, 1870
Csp6I	GTAC	5 cut(s)	870, 1334, 1406, 2236, 2385
CspAI	ACCGGT	1 cut(s)	327
CviQI	GTAC	5 cut(s)	870, 1334, 1406, 2236, 2385
DraI	TTTAAA	1 cut(s)	1903
EaeI	YGGCCR	2 cut(s)	421, 1741
EagI	CGGCCG	1 cut(s)	421
Eam1104I	CTCTTC	1 cut(s)	3281
EarI	CTCTTC	1 cut(s)	3281
EciI	GGCGGA	2 cut(s)	613, 680
Ecl136II	GAGCTC	1 cut(s)	391
EclXI	CGGCCG	1 cut(s)	421
Eco130I	CCWWGG	1 cut(s)	2922
Eco24I	GRGCYC	1 cut(s)	393
Eco47I	GGWCC	2 cut(s)	325, 397
Eco52I	CGGCCG	1 cut(s)	421
Eco53kI	GAGCTC	1 cut(s)	391
Eco57I	CTGAAG	5 cut(s)	1368, 1947, 2696, 3114, 3249
Eco72I	CACGTG	1 cut(s)	2405
Eco88I	CYCGRG	1 cut(s)	2259
Eco91I	GGTNACC	1 cut(s)	2210
EcoICRI	GAGCTC	1 cut(s)	391
EcoO65I	GGTNACC	1 cut(s)	2210
EcoRI	GAATTC	1 cut(s)	51
EcoRII	CCWGG	6 cut(s)	212, 1564, 1621, 1762, 2004, 2387
EcoT14I	CCWWGG	1 cut(s)	2922
EcoT38I	GRGCYC	1 cut(s)	393
ErhI	CCWWGG	1 cut(s)	2922
FalI	AAGNNNNNCTT	4 cut(s)	1185, 1217, 2151, 2183
FaqI	GGGAC	4 cut(s)	301, 410, 1441, 2782
FauI	CCCGC	5 cut(s)	24, 65, 96, 134, 2135
FbaI	TGATCA	2 cut(s)	1710, 2488
FblI	GTMKAC	1 cut(s)	1207
FokI	GGATG	4 cut(s)	2523, 2696, 2834, 2956
FriOI	GRGCYC	1 cut(s)	393
FspBI	CTAG	5 cut(s)	290, 1215, 1359, 2555, 2574
GlaI	GCGC	3 cut(s)	20, 288, 2661
GsaI	CCCAGC	3 cut(s)	829, 2287, 3171
GsuI	CTGGAG	2 cut(s)	222, 3059
HaeIII	GGCC	5 cut(s)	212, 217, 257, 423, 1743
HgaI	GACGC	2 cut(s)	197, 1870
HhaI	GCGC	3 cut(s)	21, 289, 2662
Hin1I	GRCGYC	2 cut(s)	189, 1881
Hin6I	GCGC	3 cut(s)	19, 287, 2660
HinP1I	GCGC	3 cut(s)	19, 287, 2660
HincII	GTYRAC	4 cut(s)	1208, 2001, 2727, 3190
HindII	GTYRAC	4 cut(s)	1208, 2001, 2727, 3190
HindIII	AAGCTT	3 cut(s)	2165, 2708, 2905
HpaI	GTTAAC	1 cut(s)	2001
Hpy166II	GTNNAC	9 cut(s)	872, 1208, 1220, 1408, 2001, 2236, 2727, 2933, 3190
Hpy8I	GTNNAC	9 cut(s)	872, 1208, 1220, 1408, 2001, 2236, 2727, 2933, 3190
Hpy99I	CGWCG	4 cut(s)	49, 191, 389, 1212
HpyAV	CCTTC	9 cut(s)	677, 767, 1343, 1929, 2003, 2234, 2550, 2685, 2761
HpyCH4III	ACNGT	6 cut(s)	838, 1212, 2332, 2746, 2777, 2981
HpyCH4IV	ACGT	1 cut(s)	2404
HpySE526I	ACGT	1 cut(s)	2404
Hsp92I	GRCGYC	2 cut(s)	189, 1881
HspAI	GCGC	3 cut(s)	19, 287, 2660
KpnI	GGTACC	1 cut(s)	2388
Ksp22I	TGATCA	2 cut(s)	1710, 2488
KspAI	GTTAAC	1 cut(s)	2001
LguI	GCTCTTC	1 cut(s)	3281
LmnI	GCTCC	9 cut(s)	396, 522, 579, 1042, 1158, 1967, 3014, 3040, 3281
Lsp1109I	GCAGC	9 cut(s)	811, 895, 937, 1102, 2120, 2142, 2520, 2533, 3257
MaeI	CTAG	5 cut(s)	290, 1215, 1359, 2555, 2574
MaeII	ACGT	1 cut(s)	2404
MfeI	CAATTG	2 cut(s)	590, 1500
MflI	RGATCY	1 cut(s)	2398
MhlI	GDGCHC	1 cut(s)	393
MluI	ACGCGT	1 cut(s)	223
MlyI	GAGTC	4 cut(s)	175, 323, 736, 2354
MmeI	TCCRAC	1 cut(s)	1437
MroXI	GAANNNNTTC	2 cut(s)	695, 2166
MslI	CAYNNNNRTG	4 cut(s)	1848, 2186, 2205, 2333
MspA1I	CMGCKG	5 cut(s)	877, 1247, 2111, 2716, 3248
MunI	CAATTG	2 cut(s)	590, 1500
Mva1269I	GAATGC	2 cut(s)	761, 2960
MvaI	CCWGG	6 cut(s)	214, 1566, 1623, 1764, 2006, 2389
MvnI	CGCG	3 cut(s)	19, 225, 1317
NciI	CCSGG	4 cut(s)	31, 73, 395, 1677
NlaIV	GGNNCC	8 cut(s)	398, 1160, 1824, 1952, 2386, 2400, 3016, 3277
NmeAIII	GCCGAG	2 cut(s)	937, 2023
NmuCI	GTSAC	6 cut(s)	832, 1060, 2072, 2210, 2420, 2629
NspI	RCATGY	1 cut(s)	2528
PaeR7I	CTCGAG	1 cut(s)	2259
PagI	TCATGA	1 cut(s)	334
PciSI	GCTCTTC	1 cut(s)	3281
PctI	GAATGC	2 cut(s)	761, 2960
PdmI	GAANNNNTTC	2 cut(s)	695, 2166
PfoI	TCCNGGA	1 cut(s)	393
PinAI	ACCGGT	1 cut(s)	327
PleI	GAGTC	4 cut(s)	174, 322, 735, 2353
PmaCI	CACGTG	1 cut(s)	2405
PmlI	CACGTG	1 cut(s)	2405
PpsI	GAGTC	4 cut(s)	174, 322, 735, 2353
Ppu21I	YACGTR	1 cut(s)	2405
PshBI	ATTAAT	1 cut(s)	2057
PsiI	TTATAA	1 cut(s)	1232
Psp124BI	GAGCTC	1 cut(s)	393
Psp6I	CCWGG	6 cut(s)	212, 1564, 1621, 1762, 2004, 2387
PspCI	CACGTG	1 cut(s)	2405
PspEI	GGTNACC	1 cut(s)	2210
PspFI	CCCAGC	3 cut(s)	825, 2283, 3167
PspGI	CCWGG	6 cut(s)	212, 1564, 1621, 1762, 2004, 2387
PspN4I	GGNNCC	8 cut(s)	398, 1160, 1824, 1952, 2386, 2400, 3016, 3277
PspPI	GGNCC	3 cut(s)	216, 325, 397
PstI	CTGCAG	1 cut(s)	1092
PstNI	CAGNNNCTG	1 cut(s)	2620
PsuI	RGATCY	1 cut(s)	2398
PvuII	CAGCTG	5 cut(s)	877, 1247, 2111, 2716, 3248
RsaI	GTAC	5 cut(s)	871, 1335, 1407, 2237, 2386
RsaNI	GTAC	5 cut(s)	870, 1334, 1406, 2236, 2385
RseI	CAYNNNNRTG	4 cut(s)	1848, 2186, 2205, 2333
SacI	GAGCTC	1 cut(s)	393
SalI	GTCGAC	1 cut(s)	1206
SapI	GCTCTTC	1 cut(s)	3281
Sau96I	GGNCC	3 cut(s)	216, 325, 397
SchI	GAGTC	4 cut(s)	175, 323, 736, 2354
SduI	GDGCHC	1 cut(s)	393
SfcI	CTRYAG	3 cut(s)	809, 1088, 3000
Sfr274I	CTCGAG	1 cut(s)	2259
SinI	GGWCC	2 cut(s)	325, 397
SlaI	CTCGAG	1 cut(s)	2259
SmiMI	CAYNNNNRTG	4 cut(s)	1848, 2186, 2205, 2333
SmlI	CTYRAG	3 cut(s)	650, 2259, 3122
SmoI	CTYRAG	3 cut(s)	650, 2259, 3122
SspI	AATATT	1 cut(s)	1271
SspMI	CTAG	5 cut(s)	290, 1215, 1359, 2555, 2574
SstI	GAGCTC	1 cut(s)	393
StyI	CCWWGG	1 cut(s)	2922
TaaI	ACNGT	6 cut(s)	838, 1212, 2332, 2746, 2777, 2981
TaiI	ACGT	1 cut(s)	2407
TatI	WGTACW	3 cut(s)	869, 1405, 2235
TauI	GCSGC	4 cut(s)	526, 762, 3236, 3276
TscAI	CASTG	3 cut(s)	553, 1351, 3178
TseFI	GTSAC	6 cut(s)	832, 1060, 2072, 2210, 2420, 2629
TseI	GCWGC	9 cut(s)	799, 908, 950, 1090, 2108, 2130, 2521, 2533, 3245
Tsp45I	GTSAC	6 cut(s)	832, 1060, 2072, 2210, 2420, 2629
TspGWI	ACGGA	1 cut(s)	459
TspRI	CASTG	3 cut(s)	553, 1351, 3178
VpaK11BI	GGWCC	2 cut(s)	325, 397
VspI	ATTAAT	1 cut(s)	2057
XceI	RCATGY	1 cut(s)	2528
XcmI	CCANNNNNNNNNTGG	2 cut(s)	250, 1562
XhoI	CTCGAG	1 cut(s)	2259
XmiI	GTMKAC	1 cut(s)	1207
XmnI	GAANNNNTTC	2 cut(s)	695, 2166
XspI	CTAG	5 cut(s)	290, 1215, 1359, 2555, 2574

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.