Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Reverse (-)

22757134 .. 22764468

Gene Structure 7335 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G018020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5169 bp

ATGAGGGGATCAAAGCCGGCCGGAATGTCGTGGCCGCAGGCTGAGCAGCGACGATTTTATCCGCAACCAGGGTCCCACCCACGCGCCGCCGTTCCGCGACGGCCGCTGCCTCGAATTCAGCGGCCGAATTTCATCGTTTATCTTCTGTCCGACAAACGGGACCACCGGCGGTCGGACATCGAGGCGGTGATCAACCAGTGCAGGGCGAAGCCGGAGAATTTCCGGGTGAGTCCGGAGAACAACACAGTCGCGTCGATGTTCTACGCGCAGTGGTACGACGCGCTCGAAGCGATGGTCTGGCTGTGGGAGTCGCGGCTCGACCGGGTTCACAATTTCACGCCCAAGCTTGACGCCAACGACTCCGTCTCCACGCCGTCCGATAGCGTGGAGCTGGAGGACCGCCTGAGAGCCTTGTTCGCCGGCCGAATTAAGCAGTTAATCGACGGCGACGAGGTGAAGAAGTGTGAAGCAAAGCGCCAAATTTTGGCCAGAGAGTACGACCGAGTTCGTAAGCTGGCCAAGAAGCCTCAGAGGAATTGGGAGGACTTACCGGAGAAGGAGAAGAGGTGCAGAGTTGAGCTTGAATTGGTTGAGAGTAGGATCAGAGAGTTCAGGTCTGCTATGAACTGCTTGCTTGCTCGTGTTGAAGGGAAAGAGTTGGAGGAGTATGGTGAAGAAGGCGTGAAGCTGTTTAAATTCGGCGAAATTTGGCATTGGAGTCGAATTCAGAGGTTAATGGTGAGGGAGTGTCGCCGACTCGATGAGGGATTGCCCATTTATGCTCATAGGCAGCAAATTCTTGAGCAGATTACTAATCAGCAGGTCATGGTGTTGATTGGAGAGACTGGTTCGGGGAAGAGTACACAATTGGTTCAGTTTCTTGCTGATTCTGGAATCGCTGCTGATAAGTCCATTGTATGCACTCAGCCTCGCAAGATTGCTGCCAATTCATTGGCAAAGAGAGTCAAGGAAGAAAGTAGTGGGTGTTACTATGGAGAAAACACAGTTACCAGTTATCAGACATCGTCTGGGCAGCAGTTGACTTCTAAGGTAACATATATGACAGACCACTGTTTACTGCAGTACTACATGAAAGATAGAAATATGACCATGATTTCTTGCATCATAGTTGATGAGGCTCATGAAAGGACCTTGAGTACAGATCTCCTTTTAGCATTGATCAAAGATTTACTGGGTCGAAGGCATCAGTTAAGGCTTATAATAATGTCTGCAACAGCTGATGCAGAAGTGCTTTCACATTATTTTTTCAACTGTAAAATCTTTCATGTAGTGGGGAGGAACTTTCCAGTTGATGTAAGATATGTTCCCTCTTTCACTGACGGAACTGCCAGTGATGTTGCTTCTTATGTTTCTGATGTGATGAGGGTAGCAAGAGAGATCCACAAGAATGAGAAAGAAGGGACAATTCTTGCATTTTTGACTTCTCAGATGGAAGTCGAATGGGTCTGCGAGAAGTTTATAACACCTGGTGCAATTGCATTGCCATTGCATGGGAAACTTTCTTTTGAAGAACAATCTAATGTTTTTCAAAATCACCCTGGGAAAAGAAAAATTATATTCGCCACAAATCTGGCGGAGACATCCCTGACAATTCCTGGGGTAAAGTATGTGATCGATTCTGGCATGGTCAAAGAAAGCAAATTTGAACCTGGAAGTGGTATGAATGTTCTCAGAGTTTGCAGGATCAGCCAGAGTTCTGCTAAGCAAAGAACAGGGCGTGCTGGAAGAACGGAACCTGGAATATGTTATAGACTTTACTCAGAATATGATTTTCAGGCGATGCCTCCATGTCAGGAGCCCGAGATCCGTAGAGTTCATCTTGGTGTAGCAGTTCTGAGGATTCTGGCTTTGGGTGTCAAGAATTTAAAAGAGTTTGAATTCATTGATGCACCTTGTTCTGAAGCTATAGACATGGCAATGAGGAATCTTGTTCAGTTAGGAGCTGTTAAGCAAAACAATGATGTCTATGAACTAACTCAAGAGGGTCGCGACTTGGTTAAATTGGGGGTTGAGCCCAGGCTTGGTAAATTAATTCTTGGCTGCTATCACCATAACTTGCGTAAGGAGGGCCTTGTTCTTGCGGCTGTAATGGCAAATGCAAGTAGCATATTTTGCAGAGTTGGTAATGACGAGGAGAAACTTAGGTCAGACTGCTTCAAGGTGCAATTTTGCCATCGTGATGGTGACCTCTTTACCCTTCTATCAGTGTACAAGATATGGGAAGCTCTGCCTCGAGAGAGGAGAAACACATGGTGTTGGGAAAACAGCATCAATGCCAAAACTATGAGGAGATGCCAGGACACTGTATCAGAATTGGAGTCTTGCCTTAAACATGAGCTCAATAGGATAATTCCTTGTAGCTGGCGTTGGAATGCGCATGTTTCAACTGATTGTGATACATACTTGAAAAAGGTTATACTTTCTTCCCTGGCAGAAAATGTAGCAATGTTCTCAGGATATGATCAAGTTGGTTATGAGGTGGCATTAACTGGGCAGCATGTTCGGCTGCACCCTTCTTGTTCATTGCTGGTATTCGGCCAGAAACCTAGTTGGGTTGTTTTTGGTGAACTCCTCTCAAGTTCTAATCAATATTTGGCCTGCGTCACTACCATTGATTTTAACTCTTTGTCTACCCTTGATCCCCCTCCTGTGTTTGATGTGTCCAAAATGGAAGGGCGAAAGTTGCAACTGAAAGTGTTGACTGGTTTTGGCAGTTGTTTGCTTAAAAGATTTTGTGGGAAGGGCAACAATTATCTGCATCATCTTGTATCTCGCGTCAGGACAATTTGCAATGACGAACTTATCAGCATTAAAGTCGATTATTATCAGAATGAGATTATGGTGTTTGCTACCCCACATAACATGGATAGGGTTATAAACCTTGTATCCGATGCATTGGAGTGTGAAAAAAGATGGCTGCATAATGAATGCTTGGAGAAATGCTTGTATCATGGTTCTGGTGGCTTGCCTCCTGTAGCATTGTTTGGGGCTGGTGCAGAGATCAAGCATCTGGAGCTTCATAAAAGGTGTTTGACTGTTGATGTATTTCATTCAAAACTGGATGACATGGATGATAAGGAGTTTCTTACTGAGCTTGAAGAGTCTGCCTCTGGTAGTATCTGTGGTCATCATAAGTTCCCCGGCACTGGGCAGGAAAATGTTGACAAAGGAAAGGGGGCCAGGTTGACATTTCTTACTCCTGATGCTGCTCAGAAAGCTGTTGAATTAAATGATTCTGAGTTTAATGGTTCTATACTGAAGGTTGTTCCTTCACAGGTTGGAGGTGATCATAAGGTCTATCCACTCCTTGCTGTTAGAGCTAAAATTTTATGGCCTCGCAGGCAAAGCCACGGGTTTGCAATTGTTAAATGTGACATGGATGACATTTACCCCATGCTTGCTGATTTTTCTAACCTTGTAGTTTTAGGAAGAAATATTCGTTGTCAGCCTAGCCAAAGATACACGGACTCTGTTGTGATTAGTGGACTAAACAGAGATCTATCTGAGAAAGAAATCCTTGATGTGTTAAGGACTGCTACTAGTAGGACAATCCTGGATTTTTTTCTGGTGAGAGGGGATGCTGTAGAAAATCCGCCATGTGGTGCATGTGAAGAAGCACTGCTAAAAGAAATATCACCCTATATGCCTAAACGGTACTCCCATAGTAGCTGCACTGTTCAGGTTTTCCAGCCTGAACCAAAGGGTCATTTCATGAGAGCTTTGATAACATTTGACGGAAGATTACACTTGGAGGCAGCAAAGGCTTTAGAACAATTGGAAGGGAAAGTATTGCCCGGCTTCCTTCCATGGCAAAAGATGGAGTGTCAGCAGTTATTTCATAGCTCTCTGTCCTGCCCTGGCCCTGTTTATCGTGTAATAAAGAAGCAGCTAGGTCCTTTACTTGAAAGTTTTACGCATCTAAAAGGTGTAGAATGTAATCTCGAGGAGTATCCTAATGGTTCCTGCAGAGTTAAGATATCAGCCAATGCTACAAAAACGATTGCTGATCTGAGGAGACGTGTGGAGGAGCTTGTGAGGGGGAAGACTATAGACCACCCAAGCCTCACTGCAACTGTTTTGCAGCTTCTGTTTTCCAGAGATGGCACCAGTCTAATGAACTCGTTACAGCGAGAGACAGGAACATATATCATTTTTGACAGGCACAAACTCAATGTACAGGTCTTTGGTGAAGCACATAAAGTTGACATGGTCAAGCAGAAATTGGTTGAATCCCTTTTAAACCTCCATGAAAGCAAGGTGCTTGAGATCCGCCTTCAAGGTAATGCTTTACCTCCTGAGTTGATGAAAGAGGTTGTTAGAAGGTTTGGGCCAGACCTCCGGGGGCTCAAAGAGAAGGTACCAGGGGCTGATTTTACTCTAAATGTACGCCGTCAATCCATTCTCATCCACGGAAGCAAGGAATTGAAGCAAAAAGTTGAAGAGATTATTGATGAGACTGCACATATGGCTGGTAGTTTGACTAAGACGATAAATAGGGAAGCTGATTGTCCAATCTGCTTGTGTGATGTTGAAGACGGTTACCGGCTTGAGGACTGTGGTCACTTGTTTTGTCGTTCATGTTTGGTGGAGCAGTGCGAGTCTGCAATCAATAATCATGATAGCTTTCCATTATGCTGCACTCATGAGGGTTGCAGGTCTCCAATTTTGATCACAGATCTCAGATCTCTTTTATCAATTGAGAAGCTGGAAGATCTCTTTAGGGCTTCGGTGGGGTCATATGTTGCTTCAAGTTGTGGCACCTACAGGTTTTGCCCATCTCCCGACTGCGCTTCAGTCTATCAGGTAGCGGCTCCTGGAACAGAAGGTGAACCATTTGTTTGTGGTGCATGTTATGCGGAGACGTGTACCATGTGCCATTTAGAACATCATCCTTACATGTCATGCAAGCAGTACAAGAATTTCAAGGAAGATCCGGATTCTTCATTGAAGGAGTGGTGCAAAGGAAAAGAACATGTGAAGAGCTGCCCGGTTTGCAGATATACAATTGAGAAGATAGATGGGTGCAACCATATAGAATGTCGGTGCGGGAAGCATATTTGCTGGGTTTGTTTGGCCTATTATGGAAGCAGCGACGAATGTTATGGCCACTTGAGAAGTGTTCACTGGCAATCATTTGAATACGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1722

Amino Acids

195.28

Weight (kDa)

7.63

Isoelectric Point (pI)

47.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	262 - 416	3.5e-10	DEAD/DEAH box helicase
AAA_19	PF13245	264 - 406	4.3e-06	AAA domain
AAA_22	PF13401	272 - 409	3.5e-06	AAA domain
Helicase_C	PF00271	464 - 584	1.5e-14	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	809 - 883	5.4e-10	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	985 - 1099	1.1e-48	DEAH11-like, RNA-binding domain
RRM_DEAH11	PF24637	1119 - 1199	3.3e-36	DEAH11 RRM domain
KH_DEAH11_1st	PF24638	1288 - 1352	3.3e-30	DEAH11 eukaryotic type KH-domain
KH_DEAH11_2nd	PF24641	1353 - 1426	8.1e-36	DEAH11 eukaryotic type KH-domain
KH_DEAH11	PF24471	1428 - 1495	9e-30	DEAH11-like, KH-domain
IBR	PF01485	1586 - 1642	3.7e-07	IBR domain, a half RING-finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1220, 1481, 2897
Acc16I	TGCGCA	1 cut(s)	2397
Acc36I	ACCTGC	2 cut(s)	811, 4665
Acc65I	GGTACC	1 cut(s)	4378
AccB1I	GGYRCC	3 cut(s)	4124, 4378, 4778
AccB7I	CCANNNNNTGG	2 cut(s)	484, 1511
AccI	GTMKAC	1 cut(s)	2651
AccII	CGCG	8 cut(s)	84, 97, 251, 266, 281, 313, 2010, 2796
AccIII	TCCGGA	2 cut(s)	232, 4954
AclWI	GGATC	8 cut(s)	16, 608, 1393, 1712, 1819, 2654, 4282, 4946
AcoI	YGGCCR	9 cut(s)	18, 32, 101, 122, 421, 486, 516, 2557, 5125
AcuI	CTGAAG	3 cut(s)	1941, 3299, 4797
AcyI	GRCGYC	1 cut(s)	351
AdeI	CACNNNGTG	2 cut(s)	1490, 2274
AflIII	ACRYGT	4 cut(s)	4039, 4883, 4917, 4993
AhlI	ACTAGT	1 cut(s)	3560
AjiI	CACGTC	2 cut(s)	4040, 4884
Alw21I	GWGCWC	1 cut(s)	2361
Alw26I	GTCTC	8 cut(s)	370, 836, 1592, 4030, 4148, 4469, 4683, 4874
AlwI	GGATC	8 cut(s)	16, 608, 1393, 1712, 1819, 2654, 4282, 4946
AlwNI	CAGNNNCTG	2 cut(s)	4108, 4388
Ama87I	CYCGRG	3 cut(s)	1820, 2253, 3962
Aor13HI	TCCGGA	2 cut(s)	232, 4954
ArsI	GACNNNNNNTTYG	2 cut(s)	279, 311
AseI	ATTAAT	1 cut(s)	2051
Asp718I	GGTACC	1 cut(s)	4378
AspLEI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4811
AspS9I	GGNCC	9 cut(s)	72, 160, 397, 1149, 2089, 3198, 3881, 3914, 4349
AsuC2I	CCSGG	6 cut(s)	224, 323, 3162, 3816, 4361, 5009
AvaI	CYCGRG	3 cut(s)	1820, 2253, 3962
AvaII	GGWCC	5 cut(s)	72, 160, 397, 1149, 3914
BalI	TGGCCA	3 cut(s)	488, 518, 5127
BanI	GGYRCC	3 cut(s)	4124, 4378, 4778
BanII	GRGCYC	4 cut(s)	1821, 2037, 2361, 4368
BauI	CACGAG	1 cut(s)	639
BbsI	GAAGAC	2 cut(s)	4070, 4560
Bbv12I	GWGCWC	1 cut(s)	2361
BccI	CCATC	9 cut(s)	286, 1444, 2195, 2202, 2928, 3832, 4115, 4804, 5033
BceAI	ACGGC	5 cut(s)	74, 116, 358, 460, 4395
BcgI	CGANNNNNNTGC	6 cut(s)	701, 735, 2504, 2538, 2818, 2852
BciVI	GTATCC	2 cut(s)	2917, 3981
BclI	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4689
BcnI	CCSGG	6 cut(s)	224, 323, 3162, 3816, 4361, 5009
BcoDI	GTCTC	8 cut(s)	370, 836, 1592, 4030, 4148, 4469, 4683, 4874
BcuI	ACTAGT	1 cut(s)	3560
BfaI	CTAG	5 cut(s)	2568, 3471, 3561, 3911, 5167
BfmI	CTRYAG	7 cut(s)	1079, 1926, 2994, 3603, 3985, 4068, 4783
BfoI	RGCGCY	1 cut(s)	478
BfuAI	ACCTGC	2 cut(s)	811, 4665
BfuI	GTATCC	2 cut(s)	2917, 3981
BglI	GCCNNNNNGGC	1 cut(s)	3361
BglII	AGATCT	5 cut(s)	1162, 3517, 4696, 4703, 4732
BlpI	GCTNAGC	2 cut(s)	42, 1722
BmcAI	AGTACT	1 cut(s)	1085
Bme18I	GGWCC	5 cut(s)	72, 160, 397, 1149, 3914
BmeT110I	CYCGRG	3 cut(s)	1820, 2253, 3962
BmgBI	CACGTC	2 cut(s)	4040, 4884
BmgT120I	GGNCC	9 cut(s)	72, 160, 397, 1149, 2089, 3198, 3881, 3914, 4349
BmrI	ACTGGG	3 cut(s)	1202, 2520, 3177
BmuI	ACTGGG	3 cut(s)	1202, 2520, 3177
BoxI	GACNNNNGTC	1 cut(s)	4578
BpiI	GAAGAC	2 cut(s)	4070, 4560
BplI	GAGNNNNNCTC	2 cut(s)	3113, 3145
BpmI	CTGGAG	2 cut(s)	413, 3053
Bpu1102I	GCTNAGC	2 cut(s)	42, 1722
BpuEI	CTTGAG	7 cut(s)	821, 1174, 1983, 2581, 4304, 4589, 5152
BpuMI	CCSGG	6 cut(s)	224, 323, 3162, 3816, 4361, 5009
Bsa29I	ATCGAT	1 cut(s)	1635
BsaBI	GATNNNNATC	1 cut(s)	2844
BsaHI	GRCGYC	1 cut(s)	351
BsaI	GGTCTC	1 cut(s)	4683
BsaWI	WCCGGW	3 cut(s)	232, 550, 4954
BsaXI	ACNNNNNCTCC	4 cut(s)	350, 380, 551, 581
Bse118I	RCCGGY	4 cut(s)	16, 165, 419, 4563
Bse3DI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
Bse8I	GATNNNNATC	1 cut(s)	2844
BseAI	TCCGGA	2 cut(s)	232, 4954
BseCI	ATCGAT	1 cut(s)	1635
BseGI	GGATG	7 cut(s)	1601, 3088, 3097, 3406, 3604, 4425, 4909
BseJI	GATNNNNATC	1 cut(s)	2844
BseMI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BseRI	GAGGAG	8 cut(s)	677, 2168, 2275, 2323, 2582, 3980, 4048, 4061
BseX3I	CGGCCG	4 cut(s)	18, 101, 122, 421
BseYI	CCCAGC	1 cut(s)	5082
BsgI	GTGCAG	7 cut(s)	220, 589, 2513, 3036, 3676, 4464, 4642
Bsh1236I	CGCG	8 cut(s)	84, 97, 251, 266, 281, 313, 2010, 2796
Bsh1285I	CGRYCG	7 cut(s)	21, 104, 125, 173, 322, 424, 502
BshNI	GGYRCC	3 cut(s)	4124, 4378, 4778
BshVI	ATCGAT	1 cut(s)	1635
BsiEI	CGRYCG	7 cut(s)	21, 104, 125, 173, 322, 424, 502
BsiHKAI	GWGCWC	1 cut(s)	2361
BsiHKCI	CYCGRG	3 cut(s)	1820, 2253, 3962
BslFI	GGGAC	3 cut(s)	58, 173, 1435
BsmAI	GTCTC	8 cut(s)	370, 836, 1592, 4030, 4148, 4469, 4683, 4874
BsmBI	CGTCTC	3 cut(s)	370, 4030, 4874
BsmFI	GGGAC	3 cut(s)	58, 173, 1435
BsmI	GAATGC	2 cut(s)	2398, 2954
Bso31I	GGTCTC	1 cut(s)	4683
BsoBI	CYCGRG	3 cut(s)	1820, 2253, 3962
Bsp1286I	GDGCHC	4 cut(s)	1821, 2037, 2361, 4368
Bsp13I	TCCGGA	2 cut(s)	232, 4954
Bsp1407I	TGTACA	2 cut(s)	2229, 4195
Bsp1720I	GCTNAGC	2 cut(s)	42, 1722
Bsp19I	CCATGG	1 cut(s)	3827
Bsp68I	TCGCGA	1 cut(s)	2010
BspDI	ATCGAT	1 cut(s)	1635
BspEI	TCCGGA	2 cut(s)	232, 4954
BspFNI	CGCG	8 cut(s)	84, 97, 251, 266, 281, 313, 2010, 2796
BspHI	TCATGA	4 cut(s)	1141, 3732, 4636, 4663
BspMAI	CTGCAG	2 cut(s)	1083, 3989
BspMI	ACCTGC	2 cut(s)	811, 4665
BspPI	GGATC	8 cut(s)	16, 608, 1393, 1712, 1819, 2654, 4282, 4946
BspQI	GCTCTTC	1 cut(s)	4994
BspT107I	GGYRCC	3 cut(s)	4124, 4378, 4778
BspTNI	GGTCTC	1 cut(s)	4683
BsrDI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BsrFI	RCCGGY	4 cut(s)	16, 165, 419, 4563
BsrGI	TGTACA	2 cut(s)	2229, 4195
BssAI	RCCGGY	4 cut(s)	16, 165, 419, 4563
BssNI	GRCGYC	1 cut(s)	351
BssSI	CACGAG	1 cut(s)	639
BssT1I	CCWWGG	1 cut(s)	3827
Bst2BI	CACGAG	1 cut(s)	639
Bst6I	CTCTTC	5 cut(s)	557, 851, 3114, 4455, 4994
BstACI	GRCGYC	1 cut(s)	351
BstAPI	GCANNNNNTGC	2 cut(s)	2133, 4874
BstAUI	TGTACA	2 cut(s)	2229, 4195
BstDSI	CCRYGG	3 cut(s)	3370, 3827, 4429
BstEII	GGTNACC	2 cut(s)	2204, 4559
BstF5I	GGATG	7 cut(s)	1601, 3088, 3097, 3406, 3604, 4425, 4909
BstFNI	CGCG	8 cut(s)	84, 97, 251, 266, 281, 313, 2010, 2796
BstH2I	RGCGCY	1 cut(s)	478
BstHHI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4811
BstMAI	GTCTC	8 cut(s)	370, 836, 1592, 4030, 4148, 4469, 4683, 4874
BstMCI	CGRYCG	7 cut(s)	21, 104, 125, 173, 322, 424, 502
BstNSI	RCATGY	6 cut(s)	2402, 2522, 3630, 4872, 4921, 4997
BstPAI	GACNNNNGTC	1 cut(s)	4578
BstPI	GGTNACC	2 cut(s)	2204, 4559
BstSFI	CTRYAG	7 cut(s)	1079, 1926, 2994, 3603, 3985, 4068, 4783
BstUI	CGCG	8 cut(s)	84, 97, 251, 266, 281, 313, 2010, 2796
BstV2I	GAAGAC	2 cut(s)	4070, 4560
BstX2I	RGATCY	9 cut(s)	1162, 1398, 1824, 3517, 4287, 4696, 4703, 4732, 4951
BstXI	CCANNNNNNTGG	3 cut(s)	952, 1591, 2201
BstYI	RGATCY	9 cut(s)	1162, 1398, 1824, 3517, 4287, 4696, 4703, 4732, 4951
BstZI	CGGCCG	4 cut(s)	18, 101, 122, 421
Bsu15I	ATCGAT	1 cut(s)	1635
BsuI	GTATCC	2 cut(s)	2917, 3981
BsuTUI	ATCGAT	1 cut(s)	1635
BtgI	CCRYGG	3 cut(s)	3370, 3827, 4429
BtgZI	GCGATG	2 cut(s)	305, 1814
BtrI	CACGTC	2 cut(s)	4040, 4884
BtsCI	GGATG	7 cut(s)	1601, 3088, 3097, 3406, 3604, 4425, 4909
BtsI	GCAGTG	4 cut(s)	275, 3638, 4086, 4619
BtuMI	TCGCGA	1 cut(s)	2010
BveI	ACCTGC	2 cut(s)	811, 4665
CaiI	CAGNNNCTG	2 cut(s)	4108, 4388
CciI	TCATGA	4 cut(s)	1141, 3732, 4636, 4663
CfoI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4811
Cfr10I	RCCGGY	4 cut(s)	16, 165, 419, 4563
Cfr13I	GGNCC	9 cut(s)	72, 160, 397, 1149, 2089, 3198, 3881, 3914, 4349
ClaI	ATCGAT	1 cut(s)	1635
CseI	GACGC	5 cut(s)	240, 287, 359, 2611, 2785
CsiI	ACCWGGT	1 cut(s)	1486
DraI	TTTAAA	3 cut(s)	694, 1887, 4260
DraIII	CACNNNGTG	2 cut(s)	1490, 2274
EaeI	YGGCCR	9 cut(s)	18, 32, 101, 122, 421, 486, 516, 2557, 5125
EagI	CGGCCG	4 cut(s)	18, 101, 122, 421
Eam1104I	CTCTTC	5 cut(s)	557, 851, 3114, 4455, 4994
EarI	CTCTTC	5 cut(s)	557, 851, 3114, 4455, 4994
EciI	GGCGGA	3 cut(s)	1610, 3603, 4280
Ecl136II	GAGCTC	1 cut(s)	2359
EclXI	CGGCCG	4 cut(s)	18, 101, 122, 421
Eco130I	CCWWGG	1 cut(s)	3827
Eco24I	GRGCYC	4 cut(s)	1821, 2037, 2361, 4368
Eco31I	GGTCTC	1 cut(s)	4683
Eco32I	GATATC	1 cut(s)	3999
Eco47I	GGWCC	5 cut(s)	72, 160, 397, 1149, 3914
Eco52I	CGGCCG	4 cut(s)	18, 101, 122, 421
Eco53kI	GAGCTC	1 cut(s)	2359
Eco57I	CTGAAG	3 cut(s)	1941, 3299, 4797
Eco88I	CYCGRG	3 cut(s)	1820, 2253, 3962
Eco91I	GGTNACC	2 cut(s)	2204, 4559
EcoICRI	GAGCTC	1 cut(s)	2359
EcoO109I	RGGNCCY	4 cut(s)	72, 1149, 2089, 3914
EcoO65I	GGTNACC	2 cut(s)	2204, 4559
EcoRI	GAATTC	3 cut(s)	114, 723, 1898
EcoRV	GATATC	1 cut(s)	3999
EcoT14I	CCWWGG	1 cut(s)	3827
EcoT22I	ATGCAT	1 cut(s)	2917
EcoT38I	GRGCYC	4 cut(s)	1821, 2037, 2361, 4368
ErhI	CCWWGG	1 cut(s)	3827
Esp3I	CGTCTC	3 cut(s)	370, 4030, 4874
FalI	AAGNNNNNCTT	2 cut(s)	3522, 3554
FaqI	GGGAC	3 cut(s)	58, 173, 1435
FauI	CCCGC	1 cut(s)	5060
FauNDI	CATATG	2 cut(s)	4485, 4759
FbaI	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4689
FblI	GTMKAC	1 cut(s)	2651
FokI	GGATG	7 cut(s)	1588, 3095, 3104, 3413, 3611, 4412, 4896
FriOI	GRGCYC	4 cut(s)	1821, 2037, 2361, 4368
FspAI	RTGCGCAY	1 cut(s)	2397
FspBI	CTAG	5 cut(s)	2568, 3471, 3561, 3911, 5167
FspI	TGCGCA	1 cut(s)	2397
GlaI	GCGC	6 cut(s)	85, 267, 282, 476, 2397, 4810
GsaI	CCCAGC	1 cut(s)	5086
GsuI	CTGGAG	2 cut(s)	413, 3053
HaeII	RGCGCY	1 cut(s)	478
HgaI	GACGC	5 cut(s)	240, 287, 359, 2611, 2785
HhaI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4811
Hin1I	GRCGYC	1 cut(s)	351
Hin6I	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4809
HinP1I	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4809
HincII	GTYRAC	5 cut(s)	1041, 2721, 3184, 3207, 4225
HindII	GTYRAC	5 cut(s)	1041, 2721, 3184, 3207, 4225
HindIII	AAGCTT	1 cut(s)	344
Hpy99I	CGWCG	7 cut(s)	54, 102, 256, 281, 446, 452, 5117
HpyCH4IV	ACGT	2 cut(s)	4039, 4883
HpySE526I	ACGT	2 cut(s)	4039, 4883
Hsp92I	GRCGYC	1 cut(s)	351
HspAI	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4809
KflI	GGGWCCC	1 cut(s)	72
Kpn2I	TCCGGA	2 cut(s)	232, 4954
KpnI	GGTACC	1 cut(s)	4382
KroI	GCCGGC	2 cut(s)	16, 419
KroNI	GCCGGC	2 cut(s)	18, 421
Ksp22I	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4689
LguI	GCTCTTC	1 cut(s)	4994
LmnI	GCTCC	7 cut(s)	388, 1816, 1961, 3034, 4048, 4609, 4837
MabI	ACCWGGT	1 cut(s)	1486
MaeI	CTAG	5 cut(s)	2568, 3471, 3561, 3911, 5167
MaeII	ACGT	2 cut(s)	4039, 4883
MaeIII	GTNAC	9 cut(s)	986, 1006, 1051, 2204, 2623, 3392, 4143, 4559, 4580
MfeI	CAATTG	6 cut(s)	866, 1494, 3381, 3794, 4716, 5025
MflI	RGATCY	9 cut(s)	1162, 1398, 1824, 3517, 4287, 4696, 4703, 4732, 4951
MhlI	GDGCHC	4 cut(s)	1821, 2037, 2361, 4368
MlsI	TGGCCA	3 cut(s)	488, 518, 5127
MluNI	TGGCCA	3 cut(s)	488, 518, 5127
MmeI	TCCRAC	5 cut(s)	153, 174, 639, 2369, 3280
Mox20I	TGGCCA	3 cut(s)	488, 518, 5127
Mph1103I	ATGCAT	1 cut(s)	2917
MroI	TCCGGA	2 cut(s)	232, 4954
MroNI	GCCGGC	2 cut(s)	16, 419
MscI	TGGCCA	3 cut(s)	488, 518, 5127
MslI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
Msp20I	TGGCCA	3 cut(s)	488, 518, 5127
MspA1I	CMGCKG	3 cut(s)	106, 121, 1238
MunI	CAATTG	6 cut(s)	866, 1494, 3381, 3794, 4716, 5025
Mva1269I	GAATGC	2 cut(s)	2398, 2954
MvnI	CGCG	8 cut(s)	84, 97, 251, 266, 281, 313, 2010, 2796
NaeI	GCCGGC	2 cut(s)	18, 421
NciI	CCSGG	6 cut(s)	224, 323, 3162, 3816, 4361, 5009
NcoI	CCATGG	1 cut(s)	3827
NdeI	CATATG	2 cut(s)	4485, 4759
NgoMIV	GCCGGC	2 cut(s)	16, 419
NmuCI	GTSAC	4 cut(s)	2204, 2623, 3392, 4580
NruI	TCGCGA	1 cut(s)	2010
NsbI	TGCGCA	1 cut(s)	2397
NsiI	ATGCAT	1 cut(s)	2917
NspI	RCATGY	6 cut(s)	2402, 2522, 3630, 4872, 4921, 4997
PaeR7I	CTCGAG	2 cut(s)	2253, 3962
PagI	TCATGA	4 cut(s)	1141, 3732, 4636, 4663
PasI	CCCWGGG	1 cut(s)	1559
PciI	ACATGT	2 cut(s)	4917, 4993
PciSI	GCTCTTC	1 cut(s)	4994
PcsI	WCGNNNNNNNCGW	2 cut(s)	282, 447
PctI	GAATGC	2 cut(s)	2398, 2954
PdiI	GCCGGC	2 cut(s)	18, 421
PfeI	GAWTC	8 cut(s)	887, 894, 1637, 1861, 1945, 3254, 4250, 4958
PflFI	GACNNNGTC	3 cut(s)	362, 1024, 4229
PflMI	CCANNNNNTGG	2 cut(s)	484, 1511
PfoI	TCCNGGA	2 cut(s)	3573, 4834
PpuMI	RGGWCCY	3 cut(s)	72, 1149, 3914
PscI	ACATGT	2 cut(s)	4917, 4993
PshAI	GACNNNNGTC	1 cut(s)	4578
PshBI	ATTAAT	1 cut(s)	2051
PsiI	TTATAA	3 cut(s)	1220, 1481, 2897
Psp124BI	GAGCTC	1 cut(s)	2361
Psp5II	RGGWCCY	3 cut(s)	72, 1149, 3914
PspEI	GGTNACC	2 cut(s)	2204, 4559
PspFI	CCCAGC	1 cut(s)	5082
PspPI	GGNCC	9 cut(s)	72, 160, 397, 1149, 2089, 3198, 3881, 3914, 4349
PspPPI	RGGWCCY	3 cut(s)	72, 1149, 3914
PsrI	GAACNNNNNNTAC	2 cut(s)	1308, 1340
PstI	CTGCAG	2 cut(s)	1083, 3989
PstNI	CAGNNNCTG	2 cut(s)	4108, 4388
PsuI	RGATCY	9 cut(s)	1162, 1398, 1824, 3517, 4287, 4696, 4703, 4732, 4951
PsyI	GACNNNGTC	3 cut(s)	362, 1024, 4229
PvuII	CAGCTG	1 cut(s)	1238
RruI	TCGCGA	1 cut(s)	2010
RseI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
SacI	GAGCTC	1 cut(s)	2361
SapI	GCTCTTC	1 cut(s)	4994
Sau96I	GGNCC	9 cut(s)	72, 160, 397, 1149, 2089, 3198, 3881, 3914, 4349
ScaI	AGTACT	1 cut(s)	1085
SduI	GDGCHC	4 cut(s)	1821, 2037, 2361, 4368
SexAI	ACCWGGT	1 cut(s)	1486
SfcI	CTRYAG	7 cut(s)	1079, 1926, 2994, 3603, 3985, 4068, 4783
Sfr274I	CTCGAG	2 cut(s)	2253, 3962
SgrAI	CRCCGGYG	1 cut(s)	165
SinI	GGWCC	5 cut(s)	72, 160, 397, 1149, 3914
SlaI	CTCGAG	2 cut(s)	2253, 3962
SmiMI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
SmlI	CTYRAG	9 cut(s)	800, 1153, 1998, 2253, 2596, 3962, 4283, 4568, 5131
SmoI	CTYRAG	9 cut(s)	800, 1153, 1998, 2253, 2596, 3962, 4283, 4568, 5131
SpeI	ACTAGT	1 cut(s)	3560
SspI	AATATT	2 cut(s)	2612, 3457
SspMI	CTAG	5 cut(s)	2568, 3471, 3561, 3911, 5167
SstI	GAGCTC	1 cut(s)	2361
StyI	CCWWGG	1 cut(s)	3827
TaiI	ACGT	2 cut(s)	4042, 4886
TaqII	GACCGA	1 cut(s)	516
TatI	WGTACW	6 cut(s)	860, 1083, 1157, 2229, 4195, 4932
TauI	GCSGC	7 cut(s)	37, 89, 106, 124, 316, 2105, 4832
TfiI	GAWTC	8 cut(s)	887, 894, 1637, 1861, 1945, 3254, 4250, 4958
TseFI	GTSAC	4 cut(s)	2204, 2623, 3392, 4580
Tsp45I	GTSAC	4 cut(s)	2204, 2623, 3392, 4580
TspGWI	ACGGA	7 cut(s)	352, 1356, 1766, 1817, 3500, 3771, 4446
Tth111I	GACNNNGTC	3 cut(s)	362, 1024, 4229
Van91I	CCANNNNNTGG	2 cut(s)	484, 1511
VpaK11BI	GGWCC	5 cut(s)	72, 160, 397, 1149, 3914
VspI	ATTAAT	1 cut(s)	2051
XceI	RCATGY	6 cut(s)	2402, 2522, 3630, 4872, 4921, 4997
XhoI	CTCGAG	2 cut(s)	2253, 3962
XmiI	GTMKAC	1 cut(s)	2651
XspI	CTAG	5 cut(s)	2568, 3471, 3561, 3911, 5167
ZrmI	AGTACT	1 cut(s)	1085
Zsp2I	ATGCAT	1 cut(s)	2917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.