Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Forward (+)

12319157 .. 12325632

Gene Structure 6476 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1036600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5166 bp

ATGAGGTCGTCGTACCCTCGCTCTACTTTCCGGCAACCTCAGCCCTCGGAAATTCCCCGCCGTTTGCCTCTCCGGCGACCCGATTACCCGCCCAACAACTTCTACCGCCAACGCCTTCCTCCCTGCCGCGCAAGCTTCATCGTCGAGCTTCTCTCCGACCACCGGAGCCTCCGGAAGCATGAAATCGACGCTGTGATCGCTAACTGCAAGTCCAGGCCCGAAGGCGTTGAGATTTCTCCCGCCAATCGCATCGTCGCCTCCTTGTTCTACACGCAATGGGTCGACGCGCTCGAAGCCCTCGTTTATTTTTGGGAGTCCCGGCTGGACCGGGTTCATGATCTCACTCCCAAGTTGAACCGGATCGTCTCTGTTCCCTCCGATCTCGACGAGCTCCGGGAGAGGCTGATAGCCCTGTTCGCCGGCCGGATTAAGAAATTGATGGACGGAGAGGCGGTGAAGAAGTGGGAGGCGAAGAGGGTCTGGTTGTGCAACGACAACGAGCGAGTTTCGAAGTTGCTTGGTGGGCATAACAATCTGTGGAATTTCAGTGAGTTGAATGAGACGATGAAGAGGAACAGGAGCGAAATTCGATTGGTTGAGAGCAGGATTAGGGAGTTCAAGTCCGCCATGAATTGCTTGCTTGATTATCTTGAGGAGAAGGGCGCGGAGGAAACTGGTGAAGAAGGCGTGAAAGTGTTCAAATTTTCGGGTGAAGATTTTGATTGGAGTCAAATTCAGAGCTTAATTGTTCGGGAATGCAGCAGGCTTGAAGAAGGGTTGCCCATTTACGCTTATCGGCAGCAAATTCTTCAGCAAATTCTTAGCCAGCAGGTCATGGTGTTGATTGGAGAGACTGGCTCAGGGAAGAGTACACAGATGGTGCAGTATCTTGCTGATTCTGGAATTGCTGCTGAGCAGTCCATTGTATGCACTCAGCCTCGTAAGATTGCTGCCATGTCGTTATCGCAAAGAGTTAAAGAAGAAAGTAGTAGGTGTTACGGAGGAAACTCAGTATCATGCAATTCAACATTTTTGTCAGGGCAGGGGTTGGTTTCTAAGGTGACATTTATGACAGATCACTGTTTACTGCAGCACTACATGAATGATACGAATATGTCAGGTATTTCTTGTATCATAGTTGATGAGGTTCATGAGAGGAGCTTGAATACTGATCTCCTGTTGGCATGTTTAAAAGATTTACTTGGTCGAAGGTCTAGTTTACGACTTATTATAATGTCAGCAACAGTTGATGCCAATGTGTTGTCCGACTATTTTTATGGTTGTGGAATATTTTCTGTAACGGGGAGGAGCTTTCCTGTTGATGTTAGATATGTACCGTGTTTCAGCGAAGGAACTTCTAGTGATGCTTCCTATGTTTCTGATGTGCTGAGGGTAGCAGCAGATGTACACAGGAAAGAGAAAGAGGGGACAATTCTTGCCTTTTTGACTTCCCAGTTGGAAGTAGAATGGTGTTGTGAGAAGTTTATAGCACCTGGTGCAATTGCATTGCCATTGCATGGAAAACTTTCTTTTGAAGACCAATTTAATGTTTTCAAAAACTACCCTGGAAAAAGGAAAATTATATTTGCCACTAATCTGGCTGAGACATCACTGACAATTCCCGGGGTAAAGTATGTGATTGATTCTGGTATGGCTAAAGAAAGCAAATTTGAACCGGGAAGTGGCATGAATGTTCTCAAAGTTTGTATGATCAGCCAGAGTTCTGCTAATCAAAGAAGTGGCCGTGCTGGAAGAACTGAACCAGGAGTATGTTATAGACTTTACTCAGAATATGACTTTGAGGCTATGCCTCCTTGTCAGGAACCTGAGATCCGCAGAGTTCATCTTGGTGTTGCAGTTCTGAGGATTCTGGCTTTAGGCGTCAAGAATTTACAGGACTTTAAATTTATCGATGCACCTTCTTCTGAAGCTATTGACTTGGCTATCAGGAACCTTGTTCAGTTGGGAGCTGTTATGCAAAAGGATGATGTCTTTGAGTTAACTCAGGAAGGTCGGTGCTTGGTTAAGTTGGGTGTTGAGCCTAGGCTTGGTAAATTAATTCTTGGTTGTTGTGACCTTAACTTACGTATCGAGGGTCTTGTTCTTGCAGCTGTAATGGCAAATTCCAGTAGCATATTTTGTAGAGTTGGTAACGACGAAGAAAAGATCAGATCAGACTGCATTAAGGTGAAATTTTGTCATCGTGATGGTGACCTCTTTACCCTTCTCACTGTGTACAAGGAATGGGATTCCTTGTCTCGAGACAAGAGAAACACATGGTGCTGGGAAAACAGCATCAATGCAAAAACTATGAGGAGATGCCAAGACACGGTGATGGAATTGGAATCTTGCCTTAAACATGAACTCAACATGATAATTTCTGGTACCTGGCGTTGGGATCCACATGAATCAACTGGTTGTGATAAACATTTGAAAAAGGTTATACTATCTTCCCTTGCAGATAATGTTGCAATGTTCTCAGGTTATGATCAACTTGGTTATGAAGTGGCATTAACTGGGCAGCATGTTAGGCTGCATCCTTCGTGTTCATTGCTAGTATTCGGGGAGAAACCTAGTTGGGTAGTCTTTGGTGAAATTCTGTCAGTTTCTAATCAGTATTTGGTGTGCGTCACCTCCTTTGATTTCAACTCTCTGTCTACGCTTTATCCTCCTCCTTCGTTTGATGTATCTAAGATGGAAAGTCGAAAGCTTCAGCTGAAAATGTTGACTGGTTTTGGCATTACTGTCCTTAAAAGATTTTGTGGGAAGGGCAACAGTTATCTGCTTCATCTCGTATCACGTATTAGGGCAATTTGCAAGGATGAACTTATCAACATCAAAGTTGATTACTGTCAGAATGAGATTATGTTGTTTGCCGCTTCACATAACATGGATAGGGTTGAAAGCTTTGTAAATGATGCCTTGGAGCGTGAACAAAAATGGATGCGTAATGAATGCTTGGAGAAATGCTTATATCACAGTTCAGGCGTGTTGCCTCCTGTAGCATTGTTTGGAGCCGGTGCTGAGATCAAACATCTGGAGCTTCAAAAAAGGTGTTTGACTTTTGATGTATTTCATTCAAAACTGGACTCTGAAGATGATAAGGAGTTACTTAGTGAGCTTGAAAAGTCTATCTCTGGCAGTATATGTGCTATTCACAAGTTCACCAGCACTGGGCAGGAAAATGTTGACAAAGGAAAGGGGGTGAGGGTAACATTTCAAACTCCTGAAGCGGCTCAAAAAGCAGCTGAGCTAAACGAATCTGAATTTAATGGCTCCATACTGAAGGTTATTCCTTCGCAGGTAGGTGGTGATCATAAAATGTTTTCATTCCCCGCTGTTAGAGCTAAGGTTTATTGGCCTCGCAGGCTAAGCAAGGGGTTTGCAATTGTTAAATGCCACATGAATGACACCCATCCCATGATTGATGACTTTTCTAACCTTGAAGTTGGAGGAAAAGTAATTCGCTGTGAGATTAGCAAAAGGGACACAGATGCTATCATGATATATGGACTAAGCAAAGATCTTTCGGAGGCTGAAATTTTAAATGTGTTGAGGACTGCTACAAGTAGGCGAATCCTAGATTTTTTCCTGCTGAGAGGAGATGCTGTAGGAAATCCGTCATGTAGTTCTTGTGAAGAAGCACTCTTAAAAGAAATATCACCCTTTATGCCTAAGAGGTATTCCCACAATAGCTGCAGTGTTCAGGTTTTCGAGCCTGAGCCAAAGAATGTTTTCATGAGAGCTTTGATTACTTTTGACGGAAGATTACACTTGGAAGCGGCGAGAGCTTTAGAAGAATTAGAAGGGAAAGTACTGCCTGGGTTCCTTCCATGGCAAAAGATGAAGTGCCAGCAGTTATTTCATAGCTCTCTATCCTGTCCTGCCCCTGTCTATCTTGTGATCAAGAAGCAGCTGGATAGTTTACTCTCAAGTTTTATGCATCTAAATGGTGTAGAATGCAATTTGGATAGAAATTCTAATGGTTCCTACCGAGTGAAGATATCTGCCAATGCTACAAAAATTGTTGCAGACCTGAGAAGACGTGTGGAGGAGCTTGTGAAAGGGAAGATTATCGACCACGTCAGCCTTACGCCAGCAGTTTTACAGATTCTTTTCTCCAGAGATGGCATCATTCTAATGCACACACTCCAGCGAGAGACAGGAACCTATATTCTTTTTGACAGACGTAGCCTGAGTGTACAGGTCTTTGGTTCTTCAGACCAAGTTGGTGTGGTCGAGAAGAAATTGGTGGACTCCCTTTTAACCCTCCACGACAATAAGCTGCTTGAGGTCCGGCTTCAAGGTAATGCCTTGCCTCCTGAGTTGATGAAAGAGGTTGTTAACAGGTTTGGGCCAGACCTCCGGGGGCTCAAAGAGAAGGTCCCAGGGGCTGATTTTAGTCTAAATGTTCGCCGTCAAGTAATTTCCATCCATGGAAGCAAGGATGTGAAGCAGAAAGTTGAAGAGAGTATTTATGAGATTGTACAGATGTCAGGTAGTTCAACTCAGAGGTTTAAGAGTGATGTTGATTGCCCCATATGTATGTGTGAGATTGAAGATGAATACCGGCTTGAGGACTGCAGTCATTTATTTTGCCGCTCGTGTTTAGTGGAGCAGTGTGAGTCAACAATTAAAAACCAAGATAGTTTCCCAATGTTTTGTGCACACAAGGGTTGCAGGTCTCTTATTTTGTTCAGTGATCTGAAATCTCTATTACCAAGTGAGAAGCTGGAGGAGCTATTTAGATCTTCATTGGGGGCGTTTGTTGCGTCAAGTGGGGGCATCTACAGGTTTTGCCCGTCTCCCGACTGCTCTTCTGTGTATCAGGTAGCTGCTCCAGGAACAGACGGTGAACCATTTGTTTGTGGTGCATGTTATGCCGAGACATGTACTAGGTGCCATTTAGAACATCATCCATACCTTTCATGTGAGCAGTACCGAGAGTTCAAGGAAGACCCAGATTCATCGTTGAAGAAGTGGTGCAAAGGAAAAGAACATGTGAAGAGCTGCCCAGTTTGCAGGTATACAATTGAGAAGATAGATGGGTGCAACCATATAGAATGTCGGTGTGGGAAGCATATTTGTTGGGTCTGTTTGGAGTATTTTGGAACGAGTGACGAATGCTATACCCATTTGAGGACTATTCACCTGGCCATCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1722

Amino Acids

194.36

Weight (kDa)

6.99

Isoelectric Point (pI)

48.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	265 - 420	6.8e-11	DEAD/DEAH box helicase
Helicase_C	PF00271	469 - 586	1.6e-13	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	811 - 882	1.8e-09	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	987 - 1101	2e-47	DEAH11-like, RNA-binding domain
RRM_DEAH11	PF24637	1121 - 1201	7.3e-36	DEAH11 RRM domain
KH_DEAH11_1st	PF24638	1290 - 1354	8.5e-33	DEAH11 eukaryotic type KH-domain
KH_DEAH11_2nd	PF24641	1355 - 1428	6.2e-35	DEAH11 eukaryotic type KH-domain
KH_DEAH11	PF24471	1430 - 1498	6.8e-30	DEAH11-like, KH-domain
IBR	PF01485	1596 - 1644	8.2e-08	IBR domain, a half RING-finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1232
Acc36I	ACCTGC	4 cut(s)	820, 3292, 4671, 5013
Acc65I	GGTACC	1 cut(s)	2384
AccB1I	GGYRCC	2 cut(s)	2384, 4899
AccB7I	CCANNNNNTGG	1 cut(s)	1517
AccBSI	CCGCTC	1 cut(s)	4602
AccI	GTMKAC	3 cut(s)	282, 2657, 5027
AccII	CGCG	3 cut(s)	129, 287, 665
AccIII	TCCGGA	1 cut(s)	171
AclWI	GGATC	4 cut(s)	368, 1825, 2393, 2406
AcoI	YGGCCR	3 cut(s)	421, 1741, 5154
AcuI	CTGAAG	7 cut(s)	794, 1947, 2696, 3114, 3249, 3305, 4203
AcyI	GRCGYC	1 cut(s)	1881
AdeI	CACNNNGTG	2 cut(s)	1496, 2280
AfiI	CCNNNNNNNGG	9 cut(s)	162, 1517, 1682, 2048, 2329, 2394, 3174, 4576, 5139
AflIII	ACRYGT	3 cut(s)	4045, 4889, 4999
AjiI	CACGTC	2 cut(s)	4046, 4084
AjnI	CCWGG	9 cut(s)	212, 1492, 1564, 1762, 2387, 3820, 4387, 4840, 5151
AloI	GAACNNNNNNTCC	2 cut(s)	1815, 1847
Alw21I	GWGCWC	2 cut(s)	393, 4669
Alw44I	GTGCAC	1 cut(s)	4665
AlwI	GGATC	4 cut(s)	368, 1825, 2393, 2406
AlwNI	CAGNNNCTG	1 cut(s)	4394
Ama87I	CYCGRG	2 cut(s)	1622, 2259
Aor13HI	TCCGGA	1 cut(s)	171
AoxI	GGCC	6 cut(s)	215, 421, 1741, 3359, 4355, 5154
ApaLI	GTGCAC	1 cut(s)	4665
ArsI	GACNNNNNNTTYG	2 cut(s)	4368, 4400
AseI	ATTAAT	1 cut(s)	2057
Asp700I	GAANNNNTTC	4 cut(s)	695, 1291, 1525, 3734
Asp718I	GGTACC	1 cut(s)	2384
AspA2I	CCTAGG	1 cut(s)	2042
AspLEI	GCGC	3 cut(s)	131, 289, 665
AspS9I	GGNCC	5 cut(s)	216, 325, 4294, 4355, 4384
AsuC2I	CCSGG	7 cut(s)	319, 329, 395, 1623, 1624, 1677, 4367
AsuII	TTCGAA	1 cut(s)	509
AvaI	CYCGRG	2 cut(s)	1622, 2259
AvaII	GGWCC	3 cut(s)	325, 4294, 4384
AvrII	CCTAGG	1 cut(s)	2042
BaeGI	GKGCMC	1 cut(s)	4669
BaeI	ACNNNNGTAYC	2 cut(s)	2071, 2104
BalI	TGGCCA	1 cut(s)	5156
BamHI	GGATCC	1 cut(s)	2398
BanI	GGYRCC	2 cut(s)	2384, 4899
BanII	GRGCYC	2 cut(s)	393, 4374
BarI	GAAGNNNNNNTAC	2 cut(s)	3798, 3830
BauI	CACGAG	1 cut(s)	4603
BbsI	GAAGAC	3 cut(s)	1542, 4048, 4962
Bbv12I	GWGCWC	2 cut(s)	393, 4669
BbvCI	CCTCAGC	2 cut(s)	39, 1388
BceAI	ACGGC	3 cut(s)	45, 1728, 4401
BciT130I	CCWGG	9 cut(s)	214, 1494, 1566, 1764, 2389, 3822, 4389, 4842, 5153
BclI	TGATCA	4 cut(s)	1710, 2488, 3313, 3903
BcnI	CCSGG	7 cut(s)	319, 329, 395, 1623, 1624, 1677, 4367
BfaI	CTAG	7 cut(s)	1215, 1359, 2043, 2555, 2574, 3581, 4896
BfmI	CTRYAG	6 cut(s)	1088, 3000, 3609, 3697, 4582, 4789
BfuAI	ACCTGC	4 cut(s)	820, 3292, 4671, 5013
BglI	GCCNNNNNGGC	2 cut(s)	73, 3367
BglII	AGATCT	2 cut(s)	3523, 4748
BlnI	CCTAGG	1 cut(s)	2042
BlpI	GCTNAGC	3 cut(s)	912, 3249, 3371
BmcAI	AGTACT	1 cut(s)	3816
Bme18I	GGWCC	3 cut(s)	325, 4294, 4384
BmeT110I	CYCGRG	2 cut(s)	1622, 2259
BmgBI	CACGTC	2 cut(s)	4046, 4084
BmgT120I	GGNCC	5 cut(s)	216, 325, 4294, 4355, 4384
BmrI	ACTGGG	4 cut(s)	1447, 2526, 3183, 5009
BmuI	ACTGGG	4 cut(s)	1447, 2526, 3183, 5009
BoxI	GACNNNNGTC	1 cut(s)	4584
BpiI	GAAGAC	3 cut(s)	1542, 4048, 4962
BplI	GAGNNNNNCTC	4 cut(s)	137, 169, 842, 874
BpmI	CTGGAG	5 cut(s)	3059, 4105, 4136, 4754, 4824
Bpu10I	CCTNAGC	5 cut(s)	39, 859, 1388, 3348, 3720
Bpu1102I	GCTNAGC	3 cut(s)	912, 3249, 3371
Bpu14I	TTCGAA	1 cut(s)	509
BpuEI	CTTGAG	4 cut(s)	671, 3916, 4310, 4595
BpuMI	CCSGG	7 cut(s)	319, 329, 395, 1623, 1624, 1677, 4367
Bsa29I	ATCGAT	1 cut(s)	1911
BsaAI	YACGTR	2 cut(s)	2087, 2801
BsaHI	GRCGYC	1 cut(s)	1881
BsaI	GGTCTC	1 cut(s)	4689
BsaWI	WCCGGW	3 cut(s)	162, 171, 357
BsaXI	ACNNNNNCTCC	8 cut(s)	438, 468, 605, 635, 3444, 3474, 3594, 3624
Bsc4I	CCNNNNNNNGG	9 cut(s)	162, 1517, 1682, 2048, 2329, 2394, 3174, 4576, 5139
Bse118I	RCCGGY	3 cut(s)	419, 3017, 4569
Bse3DI	GCAATG	5 cut(s)	281, 1505, 1511, 2478, 2549
BseAI	TCCGGA	1 cut(s)	171
BseBI	CCWGG	9 cut(s)	214, 1494, 1566, 1764, 2389, 3822, 4389, 4842, 5153
BseCI	ATCGAT	1 cut(s)	1911
BseGI	GGATG	8 cut(s)	1990, 2536, 2827, 2949, 3415, 4431, 4453, 4915
BseLI	CCNNNNNNNGG	9 cut(s)	162, 1517, 1682, 2048, 2329, 2394, 3174, 4576, 5139
BseMI	GCAATG	5 cut(s)	281, 1505, 1511, 2478, 2549
BseRI	GAGGAG	8 cut(s)	668, 1171, 1321, 2329, 2661, 3615, 4067, 4751
BseSI	GKGCMC	1 cut(s)	4669
BseX3I	CGGCCG	1 cut(s)	421
BseYI	CCCAGC	1 cut(s)	2283
BsgI	GTGCAG	1 cut(s)	902
Bsh1236I	CGCG	3 cut(s)	129, 287, 665
Bsh1285I	CGRYCG	1 cut(s)	424
BshFI	GGCC	6 cut(s)	217, 423, 1743, 3361, 4357, 5156
BshNI	GGYRCC	2 cut(s)	2384, 4899
BshVI	ATCGAT	1 cut(s)	1911
BsiEI	CGRYCG	1 cut(s)	424
BsiHKAI	GWGCWC	2 cut(s)	393, 4669
BsiHKCI	CYCGRG	2 cut(s)	1622, 2259
BslFI	GGGAC	4 cut(s)	301, 1441, 3500, 4370
BslI	CCNNNNNNNGG	9 cut(s)	162, 1517, 1682, 2048, 2329, 2394, 3174, 4576, 5139
BsmBI	CGTCTC	3 cut(s)	370, 554, 4809
BsmFI	GGGAC	4 cut(s)	301, 1441, 3500, 4370
BsmI	GAATGC	4 cut(s)	761, 2960, 3965, 5129
BsnI	GGCC	6 cut(s)	217, 423, 1743, 3361, 4357, 5156
Bso31I	GGTCTC	1 cut(s)	4689
BsoBI	CYCGRG	2 cut(s)	1622, 2259
Bsp119I	TTCGAA	1 cut(s)	509
Bsp1286I	GDGCHC	3 cut(s)	393, 4374, 4669
Bsp13I	TCCGGA	1 cut(s)	171
Bsp1407I	TGTACA	4 cut(s)	1405, 2235, 4201, 4486
Bsp1720I	GCTNAGC	3 cut(s)	912, 3249, 3371
Bsp19I	CCATGG	2 cut(s)	3833, 4435
BspANI	GGCC	6 cut(s)	217, 423, 1743, 3361, 4357, 5156
BspDI	ATCGAT	1 cut(s)	1911
BspEI	TCCGGA	1 cut(s)	171
BspFNI	CGCG	3 cut(s)	129, 287, 665
BspHI	TCATGA	4 cut(s)	334, 1150, 3501, 3738
BspMAI	CTGCAG	3 cut(s)	1092, 3701, 4586
BspMI	ACCTGC	4 cut(s)	820, 3292, 4671, 5013
BspPI	GGATC	4 cut(s)	368, 1825, 2393, 2406
BspQI	GCTCTTC	2 cut(s)	4822, 5000
BspT104I	TTCGAA	1 cut(s)	509
BspT107I	GGYRCC	2 cut(s)	2384, 4899
BspTNI	GGTCTC	1 cut(s)	4689
BsrBI	CCGCTC	1 cut(s)	4602
BsrDI	GCAATG	5 cut(s)	281, 1505, 1511, 2478, 2549
BsrFI	RCCGGY	3 cut(s)	419, 3017, 4569
BsrGI	TGTACA	4 cut(s)	1405, 2235, 4201, 4486
BssAI	RCCGGY	3 cut(s)	419, 3017, 4569
BssNAI	GTATAC	1 cut(s)	5028
BssNI	GRCGYC	1 cut(s)	1881
BssSI	CACGAG	1 cut(s)	4603
BssT1I	CCWWGG	4 cut(s)	2042, 2922, 3833, 4435
Bst1107I	GTATAC	1 cut(s)	5028
Bst2BI	CACGAG	1 cut(s)	4603
Bst2UI	CCWGG	9 cut(s)	214, 1494, 1566, 1764, 2389, 3822, 4389, 4842, 5153
Bst6I	CTCTTC	6 cut(s)	467, 563, 860, 4461, 4822, 5000
BstACI	GRCGYC	1 cut(s)	1881
BstAPI	GCANNNNNTGC	2 cut(s)	1496, 4880
BstAUI	TGTACA	4 cut(s)	1405, 2235, 4201, 4486
BstBAI	YACGTR	2 cut(s)	2087, 2801
BstBI	TTCGAA	1 cut(s)	509
BstC8I	GCNNGC	8 cut(s)	133, 421, 638, 764, 827, 3368, 3854, 4098
BstDSI	CCRYGG	2 cut(s)	3833, 4435
BstEII	GGTNACC	1 cut(s)	2210
BstF5I	GGATG	8 cut(s)	1990, 2536, 2827, 2949, 3415, 4431, 4453, 4915
BstFNI	CGCG	3 cut(s)	129, 287, 665
BstHHI	GCGC	3 cut(s)	131, 289, 665
BstMCI	CGRYCG	1 cut(s)	424
BstNI	CCWGG	9 cut(s)	214, 1494, 1566, 1764, 2389, 3822, 4389, 4842, 5153
BstNSI	RCATGY	5 cut(s)	1188, 2528, 4878, 4893, 5003
BstPAI	GACNNNNGTC	1 cut(s)	4584
BstPI	GGTNACC	1 cut(s)	2210
BstSFI	CTRYAG	6 cut(s)	1088, 3000, 3609, 3697, 4582, 4789
BstSLI	GKGCMC	1 cut(s)	4669
BstSNI	TACGTA	1 cut(s)	2087
BstUI	CGCG	3 cut(s)	129, 287, 665
BstV2I	GAAGAC	3 cut(s)	1542, 4048, 4962
BstX2I	RGATCY	4 cut(s)	1830, 2398, 3523, 4748
BstXI	CCANNNNNNTGG	1 cut(s)	1597
BstYI	RGATCY	4 cut(s)	1830, 2398, 3523, 4748
BstZ17I	GTATAC	1 cut(s)	5028
BstZI	CGGCCG	1 cut(s)	421
Bsu15I	ATCGAT	1 cut(s)	1911
BsuRI	GGCC	6 cut(s)	217, 423, 1743, 3361, 4357, 5156
BsuTUI	ATCGAT	1 cut(s)	1911
BtgI	CCRYGG	2 cut(s)	3833, 4435
BtrI	CACGTC	2 cut(s)	4046, 4084
BtsCI	GGATG	8 cut(s)	1990, 2536, 2827, 2949, 3415, 4431, 4453, 4915
BtsI	GCAGTG	2 cut(s)	3706, 4625
BtsIMutI	CAGTG	8 cut(s)	553, 1078, 1610, 2229, 3171, 3706, 4625, 4705
BveI	ACCTGC	4 cut(s)	820, 3292, 4671, 5013
Cac8I	GCNNGC	8 cut(s)	133, 421, 638, 764, 827, 3368, 3854, 4098
CaiI	CAGNNNCTG	1 cut(s)	4394
CciI	TCATGA	4 cut(s)	334, 1150, 3501, 3738
CfoI	GCGC	3 cut(s)	131, 289, 665
Cfr10I	RCCGGY	3 cut(s)	419, 3017, 4569
Cfr13I	GGNCC	5 cut(s)	216, 325, 4294, 4355, 4384
Cfr9I	CCCGGG	1 cut(s)	1622
ClaI	ATCGAT	1 cut(s)	1911
CseI	GACGC	5 cut(s)	197, 293, 1870, 2617, 4761
CsiI	ACCWGGT	1 cut(s)	1492
DraI	TTTAAA	3 cut(s)	1191, 1903, 3546
DraIII	CACNNNGTG	2 cut(s)	1496, 2280
EaeI	YGGCCR	3 cut(s)	421, 1741, 5154
EagI	CGGCCG	1 cut(s)	421
Eam1104I	CTCTTC	6 cut(s)	467, 563, 860, 4461, 4822, 5000
EarI	CTCTTC	6 cut(s)	467, 563, 860, 4461, 4822, 5000
EciI	GGCGGA	1 cut(s)	613
Ecl136II	GAGCTC	1 cut(s)	391
EclXI	CGGCCG	1 cut(s)	421
Eco105I	TACGTA	1 cut(s)	2087
Eco130I	CCWWGG	4 cut(s)	2042, 2922, 3833, 4435
Eco24I	GRGCYC	2 cut(s)	393, 4374
Eco31I	GGTCTC	1 cut(s)	4689
Eco32I	GATATC	1 cut(s)	4005
Eco47I	GGWCC	3 cut(s)	325, 4294, 4384
Eco52I	CGGCCG	1 cut(s)	421
Eco53kI	GAGCTC	1 cut(s)	391
Eco57I	CTGAAG	7 cut(s)	794, 1947, 2696, 3114, 3249, 3305, 4203
Eco88I	CYCGRG	2 cut(s)	1622, 2259
Eco91I	GGTNACC	1 cut(s)	2210
EcoICRI	GAGCTC	1 cut(s)	391
EcoO109I	RGGNCCY	1 cut(s)	4384
EcoO65I	GGTNACC	1 cut(s)	2210
EcoRII	CCWGG	9 cut(s)	212, 1492, 1564, 1762, 2387, 3820, 4387, 4840, 5151
EcoRV	GATATC	1 cut(s)	4005
EcoT14I	CCWWGG	4 cut(s)	2042, 2922, 3833, 4435
EcoT22I	ATGCAT	1 cut(s)	3945
EcoT38I	GRGCYC	2 cut(s)	393, 4374
ErhI	CCWWGG	4 cut(s)	2042, 2922, 3833, 4435
Esp3I	CGTCTC	3 cut(s)	370, 554, 4809
FalI	AAGNNNNNCTT	2 cut(s)	1185, 1217
FaqI	GGGAC	4 cut(s)	301, 1441, 3500, 4370
FauI	CCCGC	4 cut(s)	65, 96, 247, 3343
FauNDI	CATATG	1 cut(s)	4541
FbaI	TGATCA	4 cut(s)	1710, 2488, 3313, 3903
FblI	GTMKAC	3 cut(s)	282, 2657, 5027
FokI	GGATG	8 cut(s)	1997, 2523, 2834, 2956, 3402, 4418, 4460, 4902
FriOI	GRGCYC	2 cut(s)	393, 4374
FspBI	CTAG	7 cut(s)	1215, 1359, 2043, 2555, 2574, 3581, 4896
GlaI	GCGC	3 cut(s)	130, 288, 664
GsaI	CCCAGC	1 cut(s)	2287
GsuI	CTGGAG	5 cut(s)	3059, 4105, 4136, 4754, 4824
HaeIII	GGCC	6 cut(s)	217, 423, 1743, 3361, 4357, 5156
HgaI	GACGC	5 cut(s)	197, 293, 1870, 2617, 4761
HhaI	GCGC	3 cut(s)	131, 289, 665
Hin1I	GRCGYC	1 cut(s)	1881
Hin6I	GCGC	3 cut(s)	129, 287, 663
HinP1I	GCGC	3 cut(s)	129, 287, 663
HincII	GTYRAC	6 cut(s)	283, 2001, 2727, 3190, 4345, 4629
HindII	GTYRAC	6 cut(s)	283, 2001, 2727, 3190, 4345, 4629
HindIII	AAGCTT	3 cut(s)	133, 2708, 2905
HpaI	GTTAAC	2 cut(s)	2001, 4345
Hpy99I	CGWCG	7 cut(s)	13, 146, 191, 257, 287, 389, 2159
HpyCH4IV	ACGT	5 cut(s)	2086, 2800, 4045, 4083, 4189
HpySE526I	ACGT	5 cut(s)	2086, 2800, 4045, 4083, 4189
Hsp92I	GRCGYC	1 cut(s)	1881
HspAI	GCGC	3 cut(s)	129, 287, 663
Kpn2I	TCCGGA	1 cut(s)	171
KpnI	GGTACC	1 cut(s)	2388
KroI	GCCGGC	1 cut(s)	419
KroNI	GCCGGC	1 cut(s)	421
Ksp22I	TGATCA	4 cut(s)	1710, 2488, 3313, 3903
KspAI	GTTAAC	2 cut(s)	2001, 4345
LguI	GCTCTTC	2 cut(s)	4822, 5000
MabI	ACCWGGT	1 cut(s)	1492
MaeI	CTAG	7 cut(s)	1215, 1359, 2043, 2555, 2574, 3581, 4896
MaeII	ACGT	5 cut(s)	2086, 2800, 4045, 4083, 4189
MbiI	CCGCTC	1 cut(s)	4602
MfeI	CAATTG	3 cut(s)	1500, 3387, 5031
MflI	RGATCY	4 cut(s)	1830, 2398, 3523, 4748
MhlI	GDGCHC	3 cut(s)	393, 4374, 4669
MlsI	TGGCCA	1 cut(s)	5156
MluNI	TGGCCA	1 cut(s)	5156
MlyI	GAGTC	5 cut(s)	323, 736, 3083, 4250, 4634
MmeI	TCCRAC	4 cut(s)	180, 1290, 1437, 3430
Mox20I	TGGCCA	1 cut(s)	5156
Mph1103I	ATGCAT	1 cut(s)	3945
MroI	TCCGGA	1 cut(s)	171
MroNI	GCCGGC	1 cut(s)	419
MroXI	GAANNNNTTC	4 cut(s)	695, 1291, 1525, 3734
MscI	TGGCCA	1 cut(s)	5156
MslI	CAYNNNNRTG	8 cut(s)	1848, 2186, 2205, 2333, 3405, 3948, 4139, 4544
Msp20I	TGGCCA	1 cut(s)	5156
MspA1I	CMGCKG	5 cut(s)	2111, 2716, 3248, 3338, 3916
MunI	CAATTG	3 cut(s)	1500, 3387, 5031
Mva1269I	GAATGC	4 cut(s)	761, 2960, 3965, 5129
MvaI	CCWGG	9 cut(s)	214, 1494, 1566, 1764, 2389, 3822, 4389, 4842, 5153
MvnI	CGCG	3 cut(s)	129, 287, 665
NaeI	GCCGGC	1 cut(s)	421
NciI	CCSGG	7 cut(s)	319, 329, 395, 1623, 1624, 1677, 4367
NcoI	CCATGG	2 cut(s)	3833, 4435
NdeI	CATATG	1 cut(s)	4541
NgoMIV	GCCGGC	1 cut(s)	419
NmeAIII	GCCGAG	1 cut(s)	4909
NmuCI	GTSAC	5 cut(s)	1060, 2072, 2210, 2629, 5117
NsiI	ATGCAT	1 cut(s)	3945
NspI	RCATGY	5 cut(s)	1188, 2528, 4878, 4893, 5003
NspV	TTCGAA	1 cut(s)	509
PaeR7I	CTCGAG	1 cut(s)	2259
PagI	TCATGA	4 cut(s)	334, 1150, 3501, 3738
PasI	CCCWGGG	1 cut(s)	4388
PciI	ACATGT	2 cut(s)	4889, 4999
PciSI	GCTCTTC	2 cut(s)	4822, 5000
PcsI	WCGNNNNNNNCGW	2 cut(s)	288, 297
PctI	GAATGC	4 cut(s)	761, 2960, 3965, 5129
PdiI	GCCGGC	1 cut(s)	421
PdmI	GAANNNNTTC	4 cut(s)	695, 1291, 1525, 3734
PflFI	GACNNNGTC	1 cut(s)	4082
PflMI	CCANNNNNTGG	1 cut(s)	1517
PfoI	TCCNGGA	2 cut(s)	393, 4840
PleI	GAGTC	5 cut(s)	322, 735, 3083, 4250, 4633
PpsI	GAGTC	5 cut(s)	322, 735, 3083, 4250, 4633
Ppu21I	YACGTR	2 cut(s)	2087, 2801
PpuMI	RGGWCCY	1 cut(s)	4384
PscI	ACATGT	2 cut(s)	4889, 4999
PshAI	GACNNNNGTC	1 cut(s)	4584
PshBI	ATTAAT	1 cut(s)	2057
PsiI	TTATAA	1 cut(s)	1232
Psp124BI	GAGCTC	1 cut(s)	393
Psp5II	RGGWCCY	1 cut(s)	4384
Psp6I	CCWGG	9 cut(s)	212, 1492, 1564, 1762, 2387, 3820, 4387, 4840, 5151
PspEI	GGTNACC	1 cut(s)	2210
PspFI	CCCAGC	1 cut(s)	2283
PspGI	CCWGG	9 cut(s)	212, 1492, 1564, 1762, 2387, 3820, 4387, 4840, 5151
PspPI	GGNCC	5 cut(s)	216, 325, 4294, 4355, 4384
PspPPI	RGGWCCY	1 cut(s)	4384
PsrI	GAACNNNNNNTAC	2 cut(s)	4931, 4963
PstI	CTGCAG	3 cut(s)	1092, 3701, 4586
PstNI	CAGNNNCTG	1 cut(s)	4394
PsuI	RGATCY	4 cut(s)	1830, 2398, 3523, 4748
PsyI	GACNNNGTC	1 cut(s)	4082
PvuII	CAGCTG	4 cut(s)	2111, 2716, 3248, 3916
RseI	CAYNNNNRTG	8 cut(s)	1848, 2186, 2205, 2333, 3405, 3948, 4139, 4544
SacI	GAGCTC	1 cut(s)	393
SalI	GTCGAC	1 cut(s)	281
SapI	GCTCTTC	2 cut(s)	4822, 5000
Sau96I	GGNCC	5 cut(s)	216, 325, 4294, 4355, 4384
ScaI	AGTACT	1 cut(s)	3816
SchI	GAGTC	5 cut(s)	323, 736, 3083, 4250, 4634
SduI	GDGCHC	3 cut(s)	393, 4374, 4669
SexAI	ACCWGGT	1 cut(s)	1492
SfcI	CTRYAG	6 cut(s)	1088, 3000, 3609, 3697, 4582, 4789
Sfr274I	CTCGAG	1 cut(s)	2259
SfuI	TTCGAA	1 cut(s)	509
SinI	GGWCC	3 cut(s)	325, 4294, 4384
SlaI	CTCGAG	1 cut(s)	2259
SmaI	CCCGGG	1 cut(s)	1624
SmiMI	CAYNNNNRTG	8 cut(s)	1848, 2186, 2205, 2333, 3405, 3948, 4139, 4544
SmlI	CTYRAG	5 cut(s)	650, 2259, 3931, 4289, 4574
SmoI	CTYRAG	5 cut(s)	650, 2259, 3931, 4289, 4574
SnaBI	TACGTA	1 cut(s)	2087
SspI	AATATT	1 cut(s)	1290
SspMI	CTAG	7 cut(s)	1215, 1359, 2043, 2555, 2574, 3581, 4896
SstI	GAGCTC	1 cut(s)	393
StyI	CCWWGG	4 cut(s)	2042, 2922, 3833, 4435
TaiI	ACGT	5 cut(s)	2089, 2803, 4048, 4086, 4192
TatI	WGTACW	7 cut(s)	869, 1405, 2235, 3814, 4201, 4486, 4892
TauI	GCSGC	5 cut(s)	129, 2879, 3236, 3785, 4602
TscAI	CASTG	8 cut(s)	553, 1085, 1617, 2236, 3178, 3706, 4625, 4705
TseFI	GTSAC	5 cut(s)	1060, 2072, 2210, 2629, 5117
Tsp45I	GTSAC	5 cut(s)	1060, 2072, 2210, 2629, 5117
TspGWI	ACGGA	4 cut(s)	459, 1014, 3609, 3777
TspMI	CCCGGG	1 cut(s)	1622
TspRI	CASTG	8 cut(s)	553, 1085, 1617, 2236, 3178, 3706, 4625, 4705
Tth111I	GACNNNGTC	1 cut(s)	4082
Van91I	CCANNNNNTGG	1 cut(s)	1517
VneI	GTGCAC	1 cut(s)	4665
VpaK11BI	GGWCC	3 cut(s)	325, 4294, 4384
VspI	ATTAAT	1 cut(s)	2057
XceI	RCATGY	5 cut(s)	1188, 2528, 4878, 4893, 5003
XhoI	CTCGAG	1 cut(s)	2259
XmaI	CCCGGG	1 cut(s)	1622
XmaJI	CCTAGG	1 cut(s)	2042
XmiI	GTMKAC	3 cut(s)	282, 2657, 5027
XmnI	GAANNNNTTC	4 cut(s)	695, 1291, 1525, 3734
XspI	CTAG	7 cut(s)	1215, 1359, 2043, 2555, 2574, 3581, 4896
ZrmI	AGTACT	1 cut(s)	3816
Zsp2I	ATGCAT	1 cut(s)	3945

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.