Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0011951.1

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Reverse (-)

7193 .. 13312

Gene Structure 6120 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0011951.1_g000004.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5163 bp

atgaggggatcaaagccggccggaatgtcgtggccgcaggctgagcagcgacgattttatccgcaaccagggtcccacccacgcgccgccgtcccgcgacggccggtgcctcgaattcagcggccgaatttcatcgtttatcttctgtccgacaaacgggaccaccgggggtcggacatcgaggcggtgatcaaccagtgcagggcgaagccggagaatttccgggtgagtccggagaacaacacagtcgcgtcgatgttctacgcgcagtggtacgacgcgctcgaagcgatggtctggctgtgggagtcgcggctcgaccgggttcacaatttcacgcccaagcttgacgccaacgactccgtctccacgccgtccgatagcgtggagctggaggaccgcctgagaaccttgttcgccggccgaattaagcagttaatcgacggcgacgaggtgaagaagtgtgaagcaaagcgccaaattttggccagagagtacgaccgagttcgtaagctggccaagaagcctcagaggaattgggaggacttaccggagaaggagaagaggtgcagagttgagcttgaattggttgagagtaggatcagagagttcaggtctgctatgaactgcttgcttgctcgtgttgaagggaaagagttggaggagtatggtgaagaaggcgtgaagctgtttaaattcggcgaaatttggcattggagtcgaattcagaggttaatggtgagggagtgtcgccgactcgatgagggattgcccatttatgctcataggcagcaaattcttgagcagattactaatcagcaggtcatggtgttgattggagagactggttcggggaagagtacacaattggttcagtttcttgctgattctggaatcgctgctgataagtccattgtatgcactcagcctcgcaagattgctgccaattcattggcaaagagagtcaaggaagaaagtagtgggtgttactatggagaaaacacagttaccagttatcagacatcgtctggactgcagttgacttctaaggtaacatatatgacagaccactgtttactgcagtactacatgaaagatagaaatatgaccatgatttcttgcatcatagttgatgaggctcatgaaaggaccttgagtacagatctccttttagcattgatcaaagatttactgggtcgaaggcatcagttaaggcttataataatgtctgcaacagctgatgcagaagtgctttcacattattttttcaactgtaaaatctttcatgtagtggggaggaactttccagttgatgtaagatatgctccctctttcactgacggaactgccagtaatgttgcttcttatgtttctgatgtgatgagggtagcaagagagatccacaagaatgagaaagaagggacaattcttgcatttttgacttctcagatggaagtcgaatgggtctgcgagaagtttataacacctggtgcaattgcattgccattgcatgggaaactttcttttgaagaacaatctaatgtttttcaaaatcaccctgggaaaagaaaaattatattcgccacaaatctggcggagacatccctgacaattcctggggtaaagtatgtgattgattctggcatggtcaaagaaagcaaatttgaacctggaagtggtatgaatgttctcagagtttgcaggatcagccagagttctgctaagcaaagaactgggcgtgctggaagaacggaacctggaatatgttatagactctactcagaatatgattttcaggcgatgcctccatgtcaggagcccgagatccgtagagttcatcttggtgtagcagttctgaggattctggctttgggtgtcaagaatttaaaagagtttgaattcattgatgcaccttgttctgaagctatagacatggcaatgaggaatcttgttcagttaggagctgttaagcaaaacaatgatgtctatgaactaactcaagagggtcgcgacttggttaaattgggggttgagcctaggcttggtaaattaattcttggctgctatcaccataacttgcgtaaggagggccttgttcttgcggctgtaatggcaaatgcaagtagcatattttgcagagttggtaatgacgaggagaaacttaggtcagactgcttcaaggtgcaattttgccatcgtgatggtgacctctttacccttctatcagtgtacaagatatgggaagctctgcctcgagagaggagaaacacatggtgttgggaaaacagcatcaatgccaaaactatgaggagatgccaggacactgtatcagaattggagttttgccttaaacatgagctcaataggataattccttgtagctggcgttggaatgcgcatgtttcaactgattgtgatatatacttgaaaaaggttatactttcttccctggcagaaaatgtagcaatgttctcaggatatgatcaagttggttatgaggtggcattaactgggcagcatgttcggctgcacccttcttgttcattgctggtattcggccagaaacctagttgggttgtttttggtgaactcctctcaagttctaatcaatatttggcctgtgtcactaccattgattttaactctttgtctacccttgatccccctcctgtgtttgatgtgtccaagatggaaggtcgaaagttgcaactgaaagtgttgactggttttggcagttgtttgcttaaaagattttgtgggaagggcaacaattatctgcatcatcttgtatctcgtgtcaggacaatttgcaatgatgaacttatcagcattaaagtcgattattgtcagaatgagattatggtgtttgctaccccacataacatggatagggttataaaccttgtatccgatgcattggagtgtgaaaaaagatggctgcgtaatgaatgcttggagaaatgcttgtatcatggttctggtggcttgcctcctgtagcattgtttggggctggtgcagagatcaagcatctggagcttcataaaaggtgtttgactgttgatgtatttcattcaaaactggatgacatggatgataaggagtttcttactgagcttgaagagtctgcctctggtagtatctgtggtcatcataagttccccggcactgggcaggaaaatgttgacaaaggaaagggggccaggttgacatttcttactcctgatgctgctcagaaagctgttgaattaaatgattctgagtttaatggttctatactgaaggttgttccttcacaggttggaggtgatcataaggtctatccactccttgctgttagagctaaaattttatggcctcgcaggcaaagccatgggtttgcaattgttaaatgtgacatggatgatatttaccccatgcttgctgatttttctaaccttgtagttggaggaagaaatattcgttgtcagcctagcaaaagatacacgtccgactctgttgtgattagtggactaaacagagatctttctgagaaagaaatccttgatgtgttaaggactgctactagtaggacaatcctggatttttttctggtgagaggggatgctgtagaaaatccgccatgtggtgcatgtgaagaagcactgctaaaagaaatatcaccctatatgcctaaacggtactcccatagtagctgcactgttcaggttttccagcctgaaccaaagaatcatttcatgagagctttgataacatttgacggaagattacacttggaggcagcaaaggctttagaacaattggaagggaaagtattgcccggcttccttccatggcaaaagatggagtgtcagcagttatttcatagctctctgtcctgccctggccctgtttatcgtgtaataaagaagcagctagatcctttacttgaaagttttacgcatctaaaaggtgtagaatgtaatctcgaggagtatcctaatggttcctgcagagttaagatatcagccaatgctacaaaaacgattgctgatctgaggagacgtgtggaggagcttgtgagggggaagactatagaccacccaagcctcactgcaactgttttgcagcttctgttttccagagatggcaccagtctaatgaactcgttacagcgagagacaggaacatatatcatttttgacaggcacaaactcaatgtacaggtctttggtgaagcacataaagttgacatggtcaagcagaaattggttgaatcccttttaaacctccatgaaagcaaggtgcttgagatccgccttcaaggtaatgctttacctcctgagttgatgaaagaggttgttagaaggtttgggccagacctccgggggctcaaagagaaggtaccaggggctgattttactctaaatgtacgccgtcaatccattctcatctacggaagcaaggagttgaagcaaaaagttgaagagattattgatgatactgcacatatggctggtagtttgactaagaggataaatagggaagctgattgtccaatctgcttgtgtgatgttgaagacggttaccggctggaggactgtggtcacttgttttgtcgttcatgtttggtggagcagtgcgagtctgcaatcaataatcatgatagctttccattatgctgcactcatgagggttgcaggtctccaattttgatcacagatctcagatctcttttatcaattgagaagctggaagatctctttagggcttcggtggggtcatatgttgcttcaagttgtggcacctacaggttttgcccatctcccgactgcgcttcagtctatcaggtagcggctcctggaacagaaggtgaaccatttgtttgtggtgcatgttatgcggagacgtgtaccatgtgccatttagaacatcatccttacatgtcatgcaagcagtacaagaatttcaaggaagatccggattcttcattgaaggagtggtgcaaaggaaaagaacatgtgaagagctgcccggtttgcagatatacaattgagaagatagatgggtgcaaccatatagaatgtcggtgcgggaagcatatttgctgggtttgtttggcctattatggaagcagcgatgaatgttatggccacttgagaagtgttcacctggcaatcatttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1720

Amino Acids

194.91

Weight (kDa)

7.86

Isoelectric Point (pI)

46.86

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1220, 1481, 2897
Acc16I	TGCGCA	1 cut(s)	2397
Acc36I	ACCTGC	2 cut(s)	811, 4668
Acc65I	GGTACC	1 cut(s)	4381
AccB1I	GGYRCC	4 cut(s)	106, 4127, 4381, 4781
AccB7I	CCANNNNNTGG	2 cut(s)	484, 1511
AccI	GTMKAC	1 cut(s)	2651
AccII	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
AccIII	TCCGGA	2 cut(s)	232, 4957
AclWI	GGATC	9 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3911, 4285, 4949
AcoI	YGGCCR	9 cut(s)	18, 32, 101, 122, 421, 486, 516, 2557, 5128
AcuI	CTGAAG	3 cut(s)	1941, 3299, 4800
AcyI	GRCGYC	1 cut(s)	351
AdeI	CACNNNGTG	2 cut(s)	1490, 2274
AflIII	ACRYGT	5 cut(s)	3483, 4042, 4886, 4920, 4996
AhlI	ACTAGT	1 cut(s)	3563
AjiI	CACGTC	3 cut(s)	3486, 4043, 4887
Alw21I	GWGCWC	1 cut(s)	2361
Alw26I	GTCTC	7 cut(s)	370, 836, 1592, 4033, 4151, 4686, 4877
AlwI	GGATC	9 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3911, 4285, 4949
AlwNI	CAGNNNCTG	2 cut(s)	4111, 4391
Ama87I	CYCGRG	3 cut(s)	1820, 2253, 3965
Aor13HI	TCCGGA	2 cut(s)	232, 4957
ArsI	GACNNNNNNTTYG	2 cut(s)	279, 311
AseI	ATTAAT	1 cut(s)	2051
Asp700I	GAANNNNTTC	1 cut(s)	3731
Asp718I	GGTACC	1 cut(s)	4381
AspA2I	CCTAGG	1 cut(s)	2036
AspLEI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4814
AspS9I	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
AsuC2I	CCSGG	7 cut(s)	167, 224, 323, 3162, 3819, 4364, 5012
AvaI	CYCGRG	3 cut(s)	1820, 2253, 3965
AvaII	GGWCC	4 cut(s)	72, 160, 397, 1149
AvrII	CCTAGG	1 cut(s)	2036
BalI	TGGCCA	3 cut(s)	488, 518, 5130
BanI	GGYRCC	4 cut(s)	106, 4127, 4381, 4781
BanII	GRGCYC	3 cut(s)	1821, 2361, 4371
BarI	GAAGNNNNNNTAC	2 cut(s)	1345, 1377
BauI	CACGAG	2 cut(s)	639, 2793
BbsI	GAAGAC	2 cut(s)	4073, 4563
Bbv12I	GWGCWC	1 cut(s)	2361
BceAI	ACGGC	5 cut(s)	74, 116, 358, 460, 4398
BcgI	CGANNNNNNTGC	6 cut(s)	701, 735, 2504, 2538, 2818, 2852
BciVI	GTATCC	2 cut(s)	2917, 3984
BclI	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4692
BcnI	CCSGG	7 cut(s)	167, 224, 323, 3162, 3819, 4364, 5012
BcoDI	GTCTC	7 cut(s)	370, 836, 1592, 4033, 4151, 4686, 4877
BcuI	ACTAGT	1 cut(s)	3563
BfaI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
BfmI	CTRYAG	8 cut(s)	1034, 1079, 1926, 2994, 3606, 3988, 4071, 4786
BfoI	RGCGCY	1 cut(s)	478
BfuAI	ACCTGC	2 cut(s)	811, 4668
BfuI	GTATCC	2 cut(s)	2917, 3984
BglI	GCCNNNNNGGC	1 cut(s)	3361
BglII	AGATCT	5 cut(s)	1162, 3520, 4699, 4706, 4735
BlnI	CCTAGG	1 cut(s)	2036
BlpI	GCTNAGC	2 cut(s)	42, 1722
BmcAI	AGTACT	1 cut(s)	1085
Bme18I	GGWCC	4 cut(s)	72, 160, 397, 1149
BmeT110I	CYCGRG	3 cut(s)	1820, 2253, 3965
BmgBI	CACGTC	3 cut(s)	3486, 4043, 4887
BmgT120I	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
BmrI	ACTGGG	4 cut(s)	1202, 1743, 2520, 3177
BmuI	ACTGGG	4 cut(s)	1202, 1743, 2520, 3177
BoxI	GACNNNNGTC	1 cut(s)	4581
BpiI	GAAGAC	2 cut(s)	4073, 4563
BplI	GAGNNNNNCTC	2 cut(s)	3113, 3145
BpmI	CTGGAG	3 cut(s)	413, 3053, 4592
Bpu1102I	GCTNAGC	2 cut(s)	42, 1722
BpuEI	CTTGAG	6 cut(s)	821, 1174, 1983, 2581, 4307, 5155
BpuMI	CCSGG	7 cut(s)	167, 224, 323, 3162, 3819, 4364, 5012
BsaHI	GRCGYC	1 cut(s)	351
BsaI	GGTCTC	1 cut(s)	4686
BsaWI	WCCGGW	3 cut(s)	232, 550, 4957
BsaXI	ACNNNNNCTCC	4 cut(s)	350, 380, 551, 581
Bse118I	RCCGGY	4 cut(s)	16, 103, 419, 4566
Bse3DI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BseAI	TCCGGA	2 cut(s)	232, 4957
BseGI	GGATG	6 cut(s)	1601, 3088, 3097, 3406, 3607, 4912
BseMI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BseRI	GAGGAG	8 cut(s)	677, 2168, 2275, 2323, 2582, 3983, 4051, 4064
BseX3I	CGGCCG	4 cut(s)	18, 101, 122, 421
BseYI	CCCAGC	1 cut(s)	5085
BsgI	GTGCAG	7 cut(s)	220, 589, 2513, 3036, 3679, 4467, 4645
Bsh1236I	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
Bsh1285I	CGRYCG	6 cut(s)	21, 104, 125, 322, 424, 502
BshNI	GGYRCC	4 cut(s)	106, 4127, 4381, 4781
BsiEI	CGRYCG	6 cut(s)	21, 104, 125, 322, 424, 502
BsiHKAI	GWGCWC	1 cut(s)	2361
BsiHKCI	CYCGRG	3 cut(s)	1820, 2253, 3965
BslFI	GGGAC	4 cut(s)	58, 77, 173, 1435
BsmAI	GTCTC	7 cut(s)	370, 836, 1592, 4033, 4151, 4686, 4877
BsmBI	CGTCTC	3 cut(s)	370, 4033, 4877
BsmFI	GGGAC	4 cut(s)	58, 77, 173, 1435
BsmI	GAATGC	2 cut(s)	2398, 2954
Bso31I	GGTCTC	1 cut(s)	4686
BsoBI	CYCGRG	3 cut(s)	1820, 2253, 3965
Bsp1286I	GDGCHC	3 cut(s)	1821, 2361, 4371
Bsp13I	TCCGGA	2 cut(s)	232, 4957
Bsp1407I	TGTACA	2 cut(s)	2229, 4198
Bsp1720I	GCTNAGC	2 cut(s)	42, 1722
Bsp19I	CCATGG	2 cut(s)	3370, 3830
Bsp68I	TCGCGA	1 cut(s)	2010
BspEI	TCCGGA	2 cut(s)	232, 4957
BspFNI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BspHI	TCATGA	4 cut(s)	1141, 3735, 4639, 4666
BspMAI	CTGCAG	3 cut(s)	1038, 1083, 3992
BspMI	ACCTGC	2 cut(s)	811, 4668
BspPI	GGATC	9 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3911, 4285, 4949
BspQI	GCTCTTC	1 cut(s)	4997
BspT107I	GGYRCC	4 cut(s)	106, 4127, 4381, 4781
BspTNI	GGTCTC	1 cut(s)	4686
BsrDI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BsrFI	RCCGGY	4 cut(s)	16, 103, 419, 4566
BsrGI	TGTACA	2 cut(s)	2229, 4198
BssAI	RCCGGY	4 cut(s)	16, 103, 419, 4566
BssNI	GRCGYC	1 cut(s)	351
BssSI	CACGAG	2 cut(s)	639, 2793
BssT1I	CCWWGG	3 cut(s)	2036, 3370, 3830
Bst2BI	CACGAG	2 cut(s)	639, 2793
Bst6I	CTCTTC	5 cut(s)	557, 851, 3114, 4458, 4997
BstACI	GRCGYC	1 cut(s)	351
BstAPI	GCANNNNNTGC	2 cut(s)	2133, 4877
BstAUI	TGTACA	2 cut(s)	2229, 4198
BstDSI	CCRYGG	2 cut(s)	3370, 3830
BstEII	GGTNACC	2 cut(s)	2204, 4562
BstF5I	GGATG	6 cut(s)	1601, 3088, 3097, 3406, 3607, 4912
BstFNI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BstH2I	RGCGCY	1 cut(s)	478
BstHHI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4814
BstMAI	GTCTC	7 cut(s)	370, 836, 1592, 4033, 4151, 4686, 4877
BstMCI	CGRYCG	6 cut(s)	21, 104, 125, 322, 424, 502
BstNSI	RCATGY	6 cut(s)	2402, 2522, 3633, 4875, 4924, 5000
BstPAI	GACNNNNGTC	1 cut(s)	4581
BstPI	GGTNACC	2 cut(s)	2204, 4562
BstSFI	CTRYAG	8 cut(s)	1034, 1079, 1926, 2994, 3606, 3988, 4071, 4786
BstUI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BstV2I	GAAGAC	2 cut(s)	4073, 4563
BstXI	CCANNNNNNTGG	3 cut(s)	952, 1591, 2201
BstZI	CGGCCG	4 cut(s)	18, 101, 122, 421
BsuI	GTATCC	2 cut(s)	2917, 3984
BtgI	CCRYGG	2 cut(s)	3370, 3830
BtgZI	GCGATG	3 cut(s)	305, 1814, 5130
BtrI	CACGTC	3 cut(s)	3486, 4043, 4887
BtsCI	GGATG	6 cut(s)	1601, 3088, 3097, 3406, 3607, 4912
BtsI	GCAGTG	4 cut(s)	275, 3641, 4089, 4622
BtuMI	TCGCGA	1 cut(s)	2010
BveI	ACCTGC	2 cut(s)	811, 4668
CaiI	CAGNNNCTG	2 cut(s)	4111, 4391
CciI	TCATGA	4 cut(s)	1141, 3735, 4639, 4666
CfoI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4814
Cfr10I	RCCGGY	4 cut(s)	16, 103, 419, 4566
Cfr13I	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
CseI	GACGC	3 cut(s)	240, 287, 359
CsiI	ACCWGGT	1 cut(s)	1486
DraI	TTTAAA	3 cut(s)	694, 1887, 4263
DraIII	CACNNNGTG	2 cut(s)	1490, 2274
EaeI	YGGCCR	9 cut(s)	18, 32, 101, 122, 421, 486, 516, 2557, 5128
EagI	CGGCCG	4 cut(s)	18, 101, 122, 421
Eam1104I	CTCTTC	5 cut(s)	557, 851, 3114, 4458, 4997
EarI	CTCTTC	5 cut(s)	557, 851, 3114, 4458, 4997
EciI	GGCGGA	3 cut(s)	1610, 3606, 4283
Ecl136II	GAGCTC	1 cut(s)	2359
EclXI	CGGCCG	4 cut(s)	18, 101, 122, 421
Eco130I	CCWWGG	3 cut(s)	2036, 3370, 3830
Eco24I	GRGCYC	3 cut(s)	1821, 2361, 4371
Eco31I	GGTCTC	1 cut(s)	4686
Eco32I	GATATC	1 cut(s)	4002
Eco47I	GGWCC	4 cut(s)	72, 160, 397, 1149
Eco52I	CGGCCG	4 cut(s)	18, 101, 122, 421
Eco53kI	GAGCTC	1 cut(s)	2359
Eco57I	CTGAAG	3 cut(s)	1941, 3299, 4800
Eco88I	CYCGRG	3 cut(s)	1820, 2253, 3965
Eco91I	GGTNACC	2 cut(s)	2204, 4562
EcoICRI	GAGCTC	1 cut(s)	2359
EcoO109I	RGGNCCY	3 cut(s)	72, 1149, 2089
EcoO65I	GGTNACC	2 cut(s)	2204, 4562
EcoRI	GAATTC	3 cut(s)	114, 723, 1898
EcoRV	GATATC	1 cut(s)	4002
EcoT14I	CCWWGG	3 cut(s)	2036, 3370, 3830
EcoT22I	ATGCAT	1 cut(s)	2917
EcoT38I	GRGCYC	3 cut(s)	1821, 2361, 4371
ErhI	CCWWGG	3 cut(s)	2036, 3370, 3830
Esp3I	CGTCTC	3 cut(s)	370, 4033, 4877
FalI	AAGNNNNNCTT	2 cut(s)	3525, 3557
FaqI	GGGAC	4 cut(s)	58, 77, 173, 1435
FauI	CCCGC	2 cut(s)	102, 5063
FauNDI	CATATG	2 cut(s)	4488, 4762
FbaI	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4692
FblI	GTMKAC	1 cut(s)	2651
FokI	GGATG	6 cut(s)	1588, 3095, 3104, 3413, 3614, 4899
FriOI	GRGCYC	3 cut(s)	1821, 2361, 4371
FspAI	RTGCGCAY	1 cut(s)	2397
FspBI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
FspI	TGCGCA	1 cut(s)	2397
GlaI	GCGC	6 cut(s)	85, 267, 282, 476, 2397, 4813
GsaI	CCCAGC	1 cut(s)	5089
GsuI	CTGGAG	3 cut(s)	413, 3053, 4592
HaeII	RGCGCY	1 cut(s)	478
HgaI	GACGC	3 cut(s)	240, 287, 359
HhaI	GCGC	6 cut(s)	86, 268, 283, 477, 2398, 4814
Hin1I	GRCGYC	1 cut(s)	351
Hin6I	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4812
HinP1I	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4812
HincII	GTYRAC	5 cut(s)	1041, 2721, 3184, 3207, 4228
HindII	GTYRAC	5 cut(s)	1041, 2721, 3184, 3207, 4228
HindIII	AAGCTT	1 cut(s)	344
Hpy99I	CGWCG	6 cut(s)	54, 102, 256, 281, 446, 452
HpyCH4IV	ACGT	3 cut(s)	3485, 4042, 4886
HpySE526I	ACGT	3 cut(s)	3485, 4042, 4886
Hsp92I	GRCGYC	1 cut(s)	351
HspAI	GCGC	6 cut(s)	84, 266, 281, 475, 2396, 4812
KflI	GGGWCCC	1 cut(s)	72
Kpn2I	TCCGGA	2 cut(s)	232, 4957
KpnI	GGTACC	1 cut(s)	4385
KroI	GCCGGC	2 cut(s)	16, 419
KroNI	GCCGGC	2 cut(s)	18, 421
Ksp22I	TGATCA	5 cut(s)	189, 1179, 2482, 3307, 4692
LguI	GCTCTTC	1 cut(s)	4997
LmnI	GCTCC	8 cut(s)	388, 1330, 1816, 1961, 3034, 4051, 4612, 4840
MabI	ACCWGGT	1 cut(s)	1486
MaeI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
MaeII	ACGT	3 cut(s)	3485, 4042, 4886
MaeIII	GTNAC	9 cut(s)	986, 1006, 1051, 2204, 2623, 3392, 4146, 4562, 4583
MfeI	CAATTG	6 cut(s)	866, 1494, 3381, 3797, 4719, 5028
MhlI	GDGCHC	3 cut(s)	1821, 2361, 4371
MlsI	TGGCCA	3 cut(s)	488, 518, 5130
MluNI	TGGCCA	3 cut(s)	488, 518, 5130
MmeI	TCCRAC	7 cut(s)	153, 174, 639, 2369, 3280, 3424, 3513
Mox20I	TGGCCA	3 cut(s)	488, 518, 5130
Mph1103I	ATGCAT	1 cut(s)	2917
MroI	TCCGGA	2 cut(s)	232, 4957
MroNI	GCCGGC	2 cut(s)	16, 419
MroXI	GAANNNNTTC	1 cut(s)	3731
MscI	TGGCCA	3 cut(s)	488, 518, 5130
MslI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
Msp20I	TGGCCA	3 cut(s)	488, 518, 5130
MspA1I	CMGCKG	2 cut(s)	121, 1238
MunI	CAATTG	6 cut(s)	866, 1494, 3381, 3797, 4719, 5028
Mva1269I	GAATGC	2 cut(s)	2398, 2954
MvnI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
NaeI	GCCGGC	2 cut(s)	18, 421
NciI	CCSGG	7 cut(s)	167, 224, 323, 3162, 3819, 4364, 5012
NcoI	CCATGG	2 cut(s)	3370, 3830
NdeI	CATATG	2 cut(s)	4488, 4762
NgoMIV	GCCGGC	2 cut(s)	16, 419
NmuCI	GTSAC	4 cut(s)	2204, 2623, 3392, 4583
NruI	TCGCGA	1 cut(s)	2010
NsbI	TGCGCA	1 cut(s)	2397
NsiI	ATGCAT	1 cut(s)	2917
NspI	RCATGY	6 cut(s)	2402, 2522, 3633, 4875, 4924, 5000
PaeR7I	CTCGAG	2 cut(s)	2253, 3965
PagI	TCATGA	4 cut(s)	1141, 3735, 4639, 4666
PasI	CCCWGGG	1 cut(s)	1559
PciI	ACATGT	2 cut(s)	4920, 4996
PciSI	GCTCTTC	1 cut(s)	4997
PcsI	WCGNNNNNNNCGW	2 cut(s)	282, 447
PctI	GAATGC	2 cut(s)	2398, 2954
PdiI	GCCGGC	2 cut(s)	18, 421
PdmI	GAANNNNTTC	1 cut(s)	3731
PfeI	GAWTC	9 cut(s)	887, 894, 1637, 1861, 1945, 3254, 3727, 4253, 4961
PflFI	GACNNNGTC	3 cut(s)	362, 1024, 4232
PflMI	CCANNNNNTGG	2 cut(s)	484, 1511
PfoI	TCCNGGA	2 cut(s)	3576, 4837
PpuMI	RGGWCCY	2 cut(s)	72, 1149
PscI	ACATGT	2 cut(s)	4920, 4996
PshAI	GACNNNNGTC	1 cut(s)	4581
PshBI	ATTAAT	1 cut(s)	2051
PsiI	TTATAA	3 cut(s)	1220, 1481, 2897
Psp124BI	GAGCTC	1 cut(s)	2361
Psp5II	RGGWCCY	2 cut(s)	72, 1149
PspEI	GGTNACC	2 cut(s)	2204, 4562
PspFI	CCCAGC	1 cut(s)	5085
PspPI	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
PspPPI	RGGWCCY	2 cut(s)	72, 1149
PstI	CTGCAG	3 cut(s)	1038, 1083, 3992
PstNI	CAGNNNCTG	2 cut(s)	4111, 4391
PsyI	GACNNNGTC	3 cut(s)	362, 1024, 4232
PvuII	CAGCTG	1 cut(s)	1238
RruI	TCGCGA	1 cut(s)	2010
RseI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
SacI	GAGCTC	1 cut(s)	2361
SapI	GCTCTTC	1 cut(s)	4997
Sau96I	GGNCC	8 cut(s)	72, 160, 397, 1149, 2089, 3198, 3884, 4352
ScaI	AGTACT	1 cut(s)	1085
SduI	GDGCHC	3 cut(s)	1821, 2361, 4371
SexAI	ACCWGGT	1 cut(s)	1486
SfcI	CTRYAG	8 cut(s)	1034, 1079, 1926, 2994, 3606, 3988, 4071, 4786
Sfr274I	CTCGAG	2 cut(s)	2253, 3965
SinI	GGWCC	4 cut(s)	72, 160, 397, 1149
SlaI	CTCGAG	2 cut(s)	2253, 3965
SmiMI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
SmlI	CTYRAG	8 cut(s)	800, 1153, 1998, 2253, 2596, 3965, 4286, 5134
SmoI	CTYRAG	8 cut(s)	800, 1153, 1998, 2253, 2596, 3965, 4286, 5134
SpeI	ACTAGT	1 cut(s)	3563
SspI	AATATT	2 cut(s)	2612, 3457
SspMI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
SstI	GAGCTC	1 cut(s)	2361
StyI	CCWWGG	3 cut(s)	2036, 3370, 3830
TaiI	ACGT	3 cut(s)	3488, 4045, 4889
TaqII	GACCGA	1 cut(s)	516
TatI	WGTACW	6 cut(s)	860, 1083, 1157, 2229, 4198, 4935
TauI	GCSGC	6 cut(s)	37, 89, 124, 316, 2105, 4835
TfiI	GAWTC	9 cut(s)	887, 894, 1637, 1861, 1945, 3254, 3727, 4253, 4961
TseFI	GTSAC	4 cut(s)	2204, 2623, 3392, 4583
Tsp45I	GTSAC	4 cut(s)	2204, 2623, 3392, 4583
TspGWI	ACGGA	6 cut(s)	352, 1356, 1766, 1817, 3774, 4449
Tth111I	GACNNNGTC	3 cut(s)	362, 1024, 4232
Van91I	CCANNNNNTGG	2 cut(s)	484, 1511
VpaK11BI	GGWCC	4 cut(s)	72, 160, 397, 1149
VspI	ATTAAT	1 cut(s)	2051
XceI	RCATGY	6 cut(s)	2402, 2522, 3633, 4875, 4924, 5000
XhoI	CTCGAG	2 cut(s)	2253, 3965
XmaJI	CCTAGG	1 cut(s)	2036
XmiI	GTMKAC	1 cut(s)	2651
XmnI	GAANNNNTTC	1 cut(s)	3731
XspI	CTAG	5 cut(s)	2037, 2568, 3471, 3564, 3914
ZrmI	AGTACT	1 cut(s)	1085
Zsp2I	ATGCAT	1 cut(s)	2917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.