Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Reverse (-)

23806642 .. 23812822

Gene Structure 6181 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000017968

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5181 bp

ATGAGGGGATCAAACCCGCTCACCAAGTTCCGGCAGCGGAAGCCGGCCGGAATTTCGCGGCCGCAGGCTGAGCAGCGGCTATTTTATCCGCAACCAGGGTCCCACCCACGCGGCGTCGTTCCGCGACGGCCGTTGCCTCCGATTCAGCGGCCGAATTTCATCGTTTATCTTCTGTCCGACAAACGGGACCTCCGGCGGTCGGACATCGAGGCGGTGATCAACCAGTGCAGGGCGAAGCCGGAGAATTTCCGGGTGAGTCCGGAGACCAACACAGTCGCGTCGATGTTCTACGCGCAGTGGTGCGACGCGCTCGAAGCGATGGTCTGGCTGTGGGAGTCGCGGCTCGACCGGGTTCACAATTTCATGCCCAAGCTCGACGCCAACGTCTCCGTGCCGTCGGATTGGGTGGAGCTGGAGGACCGCCTGAGAGCCTTGTTCGCCGGCCGAATTAAGCACTTAATCGACGGCGACGAGGTGAAGAAGTGTGAAGCAAAGCGCCAAAATTTGGCCAGGGAGTACGAGCGAGTTCGTAAGCTGGCCAAGAGGCCTCAGAGGAATTGGGAGGACTTACCGGAGAAGGAGAAGAGGTGCAGAGTTGAGCTTGAATTGGTTGAGAGTAGGATCAGAGAGTTCAGGTCTGCTATGAACTGTTTACTTGCTCATGTTGAAGGGAAAGAGTTGGAGGAGTATGGTGAAGAAGGCTTGAAGCTGTTTAAATTCGGCGAAATTCGGTATTGGAATCGAATTCAGAGGTTAATGATGAGGGAGTGTCGTCGACTCGATGAGGGATTGCCCATTTATGCTCATCGGCAGCAAATTCTTGAGCAGATTAATAATCAGCAGGTCATGGTGTTGATTGGAGAGACTGGTTCAGGGAAGAGTACACAATTGGTTCAGTTTCTTGCTGATTCTGGAATCGCTGCTGATAAGTCCATTGTATGCACTCAGCCTCGCAAGATTGCTGCCAATTCATTGGCAAAGAGAGTCAAAGAAGAAAGTAGTGGGTGTTACTATGGAGAAAACACAGTTACCAGTTATCAGACATCGGCTGGACAGCAGTTGACTTCTAAGGTAACATATATGACAGACCACTGTTTACTGCAGTACTACATGAAAGATAGAAATATGACCATGATTTCTTGCATCATAGTTGATGAGGCTCATGAAAGGACCTTGAGTACAGATCTCCTTTTAGCATTGATCAAAGATTTACTGGGTCGAAGGCCTCAGTTACGGCTTATAATAATGTCTGCAACAGCTGATGCAGAAGTGCTTTCACATTATTTTTTCAATTGTGAAATCTTTCATGTAGTGGGGAGGAACTTTCCAGTTGATGTAAGATATACTCCCTCTTTCACTGACGGAACTGCCAGTAATGTTGCTTCTTATGTCTCTGATGTGATGAGGGTAGCAAGAGAGATCCACAAGAATGAGAAAGAAGGGACAATTCTTGCATTTTTGACTTCTCAGATGGAAGTCGAATGGGTCTGCGAGAAGTTTATAACACCTGGTGCAATTGCATTGCCATTGCATGGGAAACTTTCTTTTGAAGAACAATCTAATGTTTTTCAAAATTACCCTGGAAAAAGAAAAATTATATTTGCCACAAATCTGGCGGAGACATCCCTGACAATTCCTGGGGTAAAGTATGTGATCGATTCTGGCATGGTCAAAGAAAGCAAATTTGAACCTGGAAGTGGTATGAATGTTCTCAGAGTTTGCAAGATCAGCCAGAGTTCTGCTAAGCAAAGAACTGGGCGTGCTGGAAGAACGGAACCTGGAATATGTTATAGACTCTACTCAGAATATGATTTTCAGGCAATGCCTCCATGTCAGGAGCCCGAGATCCGTAGAGTTCATCTTGGTGTAGCAGTTCTGAGGATTCTGGCTTTGGGTGTCAAGAATTTAAAAGAGTTTGAATTCATTGATGCACCTTGTTCTGAAGCCATAGACATGGCAATGAGGAATCTTGTTCAGTTAGGAGCTGTTAAGCAAAACAATGATGTCTATGAACTAACTCCAGAGGGTCGCCAGTTGGTTAAATTGGGGGTTGAGCCTAGGCTTGGTAAATTAATTCTTGGCTGCTATCACCATAACTTGCGTAAGGAGGGCCTTGTTCTTGCGGCTGTAATGGCAAATGCAAGTAGCATATTTTGCAGAGTTGGTAATGACGAGGAGAAACTTAGGTCAGACTGCTTCAAGGTGCAATTTTGCCATCGTGATGGTGACCTTTTTACCCTTCTATCAGTGTACAAGATATGGGAAGCTCTGCCTCGAGAGAGGAAAAACACATGGTGTTGGGAAAACAGCATCAATGCCAAAACTATGAGGAGATGCCAAGACACTGTATCGGAATTGGAGTCTTGCCTTAAACATGAACTCAATATGATAATTCCTAGTAGCTGGCGTTGGAATGCGCATGTTTCAACTGATTGTGATATATACTTGAAAAAGGTTATACTTTCTTCCCTGGCAGAAAATGTAGCAATGTTCTCAGGATATGATCAAGTTGGTTTTGAGGTGGCATTAACTGGGCAGCATGTTCGGCTGCACCCTTCTTGTTCATTGCTGGTATTTGGCCAGAAACCTAGTTGGGTTGTTTTTGGTGAACTCCTATCAAGTTCTAATCAGTATTTGGCCTGCGTCACTACCATTGATTTTAACTCTTTGTCTACCCTTGATCCCCCTCCTGTGTTTGATGTGTCCAGGATGGAAGGTCGAAAGTTGCAACTGAAAGTGTTGACTGGTTTTGGCAGTTGTTTGCTTAAAAGATTTTGTGGGAAGAGCAACAGTTATCTGCATCATCTTGTATCTCGCGTCAGGACAATTTGCAATGACGAACTTATCAGCATTAAAGTCGATTATTATCAGAATGAGATTATGGTGTTTGCTACCCCACATAACATGGATAGGGTTACAAACTTTGTATCCGATGCATTGGAGTGTGAAAGAAGATGGCTGCGTAATGAATGCTTGGAGAAATGCTTGTATCATGGTTCTGGTGGCTTGCCTCCTATAGCATTGTTTGGGGCTGGTGCAGAGATCAAGCATCTGGAGCTTCATAAAAGGTGTTTGACTGTTGATGTATTTCATTCAAAACTGGATGACTTGGATGATAAGGAGTTTCTTACTGAGCTTGAAGAGTCTGCCTCTGGTAGTATCTGTGGTCATCATAGGTTCCCCGGCACTGGGCAGGAAAATGTTGACAAAGGAAAGGGGGCCAGGTTGACATTTCTTACTCCTGATGCTGCTCAGAAAGCTGTTGAATTAAATGATTCTGAGTTTAATGGTTCTATACTGAAGGTTGTTCCTTCACAGGTTGGAGGTGATCATAAAGTCTATCCACTCCTTGCTGTTAGAGCTAAAATTTTATGGCCTCGCAGGCAAAGCAATGGGTTTGCAATTGTTAAATGTGACATGGATGATATTTACCCCATGCTTGCTGATTTTTCTAACCTTGTAGTTGGAGGAAGAAATATTCATTGTCAGCCTAGCAAAAGATACATGGACTCTGTTGTGATTAGTGGACTAAACAAAGATCTTTCTGAGAAAGAAATCCTTGATGTGTTAAACACTGGTACTAGTAGGAATATCCTGGATTTTTTTCTGGTGAGAGGGGATGCTGTAGAAAATCCGCCATGTGGTGCATGTGAAGAAGCACTGCTAAAAGAAATATCACCCTATATGCCTAAACGGTACTCCCATAGTAGCTGCAGTGTTCAGGTTTTCCAGCCTGAACCAAAGAGTCATTTCATGAGAGCTTTGATAACATTTGATGGAAGATTACACTTGGAGGCAGCAAGGGCTTTAGAACAATTGGAAGGGAAAGTATTGCCTGGCTTCCTTCCATGGCAAAAGATGCAGTGTCAGCAGTTATTTCATAGCTCTCTGTCCTGCCCTGGCCCTGTTTATCGTGTTATAAAGAAGCAGCTAGATCCTTTACTTGAAAGTTTTATGCATCTAAAAGGTGTAGAATGTAATCTCGAGGAGTATCCTAATGGTTCCTGCAGAGTTAAGATATCAGCCAATGCTACAAAAACGATTGCAGATCTGAGGAGACGTGTGGAGGAGCTTGTGAGGGGGAAGACTATAGACCACCCAAGCCTCACTGCAACTGTTTTGCAGCTTCTGTTTTCCAGAGAAGGCACCGGTCTAATGTACTCGTTACAGCGAGAGACAGGAACATATATCATTTTTGACAGGCACAAACTCAATGTACAGGTCTTTGGTGAATCACATAAAGTTGACATGGTCAAGCAGAAATTGGTTGAATCCCTTTTAAACCTCCATGAAAGCAAGGTGCTTGAGATCCGCCTTCAAGGTAATGCTTTACCTCCTGAGTTGATGAAAGAGGTTGTTAGAAGGTTTGGGCCAGACCTCCGGGGGCTCAAAGAGAAGGTACCAGGGGCTGATTTTACTCTAAATGTACGCCGTCAATCCATTCTCATCCACGGAAGCAAGGAGTTGAAGCAAAAAGTTGAAGAGATTATCGATGAGACTGCACATATGGCTGGTAGTTTGACTAAGAGGATAGATAGGGAAGCTGATTGTCCAATCTGCTTGTGTGATGTTGAAGATGGTTACCGGCTTGAGGACTGTGGTCACTTGTTTTGTCGTTCATGTTTGGTGGAGCAGTGCGAGTCTGCAATCAATAATCATGATAGCTTTCCATTATGCTGCACTCATGAGGGTTGCAGGTCTCCAATTTTGATCACGGATCTCAGATCTCTTTTATCAATTGAGAAGCTGGAAGATCTCTTTAGGGCGTCGGTGGGGTCATATGTTGCTTCAAGTTGTGGCACCTACAGGTTTTGCCCATCTCCTGACTGCGCTTCAGTCTATCAGGTAGCGGCTCCTGGAACAGAAGGTGAACCATTTGTTTGTGGTGCATGTTATGCGGAGACGTGTACCATGTGCCATTTAGAACATCATCCTTACATGTCATGCAAGCAGTACAAGAATTTCAAGGAAGATCCGGATTCTTCATTGAAGGAGTGGTGCAAAGGAAAAGAACATGTGAAGAGCTGCCCGGTTTGCAGATATACAATTGAGAAGATAGATGGGTGCAACCATATAGAATGTCGGTGCGGGAAGCATATTTGCTGGGTTTGTTTGGCCTATTATGGAAGCAGCGATGAATGTTATGGCCACTTGAGAAGTGTTCACCTGGCAATCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1727

Amino Acids

195.81

Weight (kDa)

7.86

Isoelectric Point (pI)

47.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	269 - 423	2.6e-10	DEAD/DEAH box helicase
Helicase_C	PF00271	471 - 591	5.4e-15	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	816 - 890	2.3e-09	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	992 - 1106	3.3e-48	DEAH11-like, RNA-binding domain
RRM_DEAH11	PF24637	1126 - 1206	2.8e-36	DEAH11 RRM domain
KH_DEAH11_1st	PF24638	1295 - 1359	5.9e-31	DEAH11 eukaryotic type KH-domain
KH_DEAH11_2nd	PF24641	1360 - 1433	8.7e-35	DEAH11 eukaryotic type KH-domain
KH_DEAH11	PF24471	1435 - 1502	9e-30	DEAH11-like, KH-domain
IBR	PF01485	1593 - 1649	3.7e-07	IBR domain, a half RING-finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1241, 1502, 3920
AasI	GACNNNNNNGTC	1 cut(s)	383
Acc16I	TGCGCA	1 cut(s)	2418
Acc36I	ACCTGC	2 cut(s)	832, 4686
Acc65I	GGTACC	1 cut(s)	4399
AccB1I	GGYRCC	3 cut(s)	4145, 4399, 4799
AccB7I	CCANNNNNTGG	2 cut(s)	505, 1532
AccBSI	CCGCTC	1 cut(s)	19
AccI	GTMKAC	2 cut(s)	775, 2672
AccII	CGCG	8 cut(s)	58, 111, 124, 278, 293, 308, 340, 2817
AccIII	TCCGGA	2 cut(s)	259, 4975
AclWI	GGATC	9 cut(s)	16, 629, 1414, 1840, 2675, 3929, 4303, 4725, 4967
AcoI	YGGCCR	9 cut(s)	45, 59, 128, 149, 442, 507, 537, 2578, 5146
AcuI	CTGAAG	3 cut(s)	1962, 3320, 4818
AcyI	GRCGYC	3 cut(s)	114, 378, 4766
AdeI	CACNNNGTG	2 cut(s)	1511, 2295
AflIII	ACRYGT	4 cut(s)	4060, 4904, 4938, 5014
AgeI	ACCGGT	1 cut(s)	4148
AhlI	ACTAGT	1 cut(s)	3581
AjiI	CACGTC	2 cut(s)	4061, 4905
AlwI	GGATC	9 cut(s)	16, 629, 1414, 1840, 2675, 3929, 4303, 4725, 4967
AlwNI	CAGNNNCTG	2 cut(s)	4129, 4409
Ama87I	CYCGRG	3 cut(s)	1841, 2274, 3983
Aor13HI	TCCGGA	2 cut(s)	259, 4975
ArsI	GACNNNNNNTTYG	2 cut(s)	306, 338
AseI	ATTAAT	2 cut(s)	831, 2072
AsiGI	ACCGGT	1 cut(s)	4148
Asp700I	GAANNNNTTC	1 cut(s)	1302
Asp718I	GGTACC	1 cut(s)	4399
AspA2I	CCTAGG	1 cut(s)	2057
AspLEI	GCGC	5 cut(s)	295, 310, 498, 2419, 4832
AspS9I	GGNCC	8 cut(s)	99, 187, 418, 1170, 2110, 3219, 3902, 4370
AsuC2I	CCSGG	5 cut(s)	251, 350, 3183, 4382, 5030
AvaI	CYCGRG	3 cut(s)	1841, 2274, 3983
AvaII	GGWCC	4 cut(s)	99, 187, 418, 1170
AvrII	CCTAGG	1 cut(s)	2057
BalI	TGGCCA	4 cut(s)	509, 539, 2580, 5148
BanI	GGYRCC	3 cut(s)	4145, 4399, 4799
BanII	GRGCYC	2 cut(s)	1842, 4389
BarI	GAAGNNNNNNTAC	2 cut(s)	1366, 1398
BbsI	GAAGAC	1 cut(s)	4091
BceAI	ACGGC	6 cut(s)	115, 143, 379, 481, 1250, 4416
BcgI	CGANNNNNNTGC	6 cut(s)	2525, 2559, 2839, 2873, 4481, 4515
BciVI	GTATCC	2 cut(s)	2938, 4002
BclI	TGATCA	5 cut(s)	216, 1200, 2503, 3328, 4710
BcnI	CCSGG	5 cut(s)	251, 350, 3183, 4382, 5030
BcuI	ACTAGT	1 cut(s)	3581
BfaI	CTAG	6 cut(s)	2058, 2397, 2589, 3492, 3582, 3932
BfmI	CTRYAG	7 cut(s)	1100, 3015, 3624, 3712, 4006, 4089, 4804
BfoI	RGCGCY	1 cut(s)	499
BfuAI	ACCTGC	2 cut(s)	832, 4686
BfuI	GTATCC	2 cut(s)	2938, 4002
BglI	GCCNNNNNGGC	1 cut(s)	3382
BglII	AGATCT	5 cut(s)	1183, 3538, 4048, 4724, 4753
BlnI	CCTAGG	1 cut(s)	2057
BlpI	GCTNAGC	2 cut(s)	69, 1743
BmcAI	AGTACT	1 cut(s)	1106
Bme18I	GGWCC	4 cut(s)	99, 187, 418, 1170
BmeT110I	CYCGRG	3 cut(s)	1841, 2274, 3983
BmgBI	CACGTC	2 cut(s)	4061, 4905
BmgT120I	GGNCC	8 cut(s)	99, 187, 418, 1170, 2110, 3219, 3902, 4370
BmrI	ACTGGG	4 cut(s)	1223, 1764, 2541, 3198
BmuI	ACTGGG	4 cut(s)	1223, 1764, 2541, 3198
BoxI	GACNNNNGTC	1 cut(s)	4599
BpiI	GAAGAC	1 cut(s)	4091
BplI	GAGNNNNNCTC	2 cut(s)	3134, 3166
BpmI	CTGGAG	3 cut(s)	434, 2004, 3074
Bpu1102I	GCTNAGC	2 cut(s)	69, 1743
BpuEI	CTTGAG	5 cut(s)	842, 1195, 4325, 4610, 5173
BpuMI	CCSGG	5 cut(s)	251, 350, 3183, 4382, 5030
Bsa29I	ATCGAT	2 cut(s)	1656, 4491
BsaBI	GATNNNNATC	1 cut(s)	2865
BsaHI	GRCGYC	3 cut(s)	114, 378, 4766
BsaI	GGTCTC	2 cut(s)	257, 4704
BsaWI	WCCGGW	4 cut(s)	259, 571, 4148, 4975
BsaXI	ACNNNNNCTCC	4 cut(s)	174, 204, 572, 602
Bse118I	RCCGGY	4 cut(s)	43, 440, 4148, 4584
Bse3DI	GCAATG	8 cut(s)	1520, 1526, 1827, 1965, 2493, 2564, 2839, 3397
Bse8I	GATNNNNATC	1 cut(s)	2865
BseAI	TCCGGA	2 cut(s)	259, 4975
BseCI	ATCGAT	2 cut(s)	1656, 4491
BseGI	GGATG	8 cut(s)	1622, 2715, 3109, 3118, 3427, 3625, 4446, 4930
BseJI	GATNNNNATC	1 cut(s)	2865
BseMI	GCAATG	8 cut(s)	1520, 1526, 1827, 1965, 2493, 2564, 2839, 3397
BseRI	GAGGAG	6 cut(s)	698, 2189, 2344, 4001, 4069, 4082
BseX3I	CGGCCG	5 cut(s)	45, 59, 128, 149, 442
BseYI	CCCAGC	1 cut(s)	5103
BsgI	GTGCAG	6 cut(s)	247, 610, 2534, 3057, 4485, 4663
Bsh1236I	CGCG	8 cut(s)	58, 111, 124, 278, 293, 308, 340, 2817
Bsh1285I	CGRYCG	7 cut(s)	48, 62, 131, 152, 200, 349, 445
BshNI	GGYRCC	3 cut(s)	4145, 4399, 4799
BshTI	ACCGGT	1 cut(s)	4148
BshVI	ATCGAT	2 cut(s)	1656, 4491
BsiEI	CGRYCG	7 cut(s)	48, 62, 131, 152, 200, 349, 445
BsiHKCI	CYCGRG	3 cut(s)	1841, 2274, 3983
BslFI	GGGAC	3 cut(s)	85, 200, 1456
BsmBI	CGTCTC	3 cut(s)	391, 4051, 4895
BsmFI	GGGAC	3 cut(s)	85, 200, 1456
BsmI	GAATGC	2 cut(s)	2419, 2975
Bso31I	GGTCTC	2 cut(s)	257, 4704
BsoBI	CYCGRG	3 cut(s)	1841, 2274, 3983
Bsp1286I	GDGCHC	2 cut(s)	1842, 4389
Bsp13I	TCCGGA	2 cut(s)	259, 4975
Bsp1407I	TGTACA	2 cut(s)	2250, 4216
Bsp1720I	GCTNAGC	2 cut(s)	69, 1743
Bsp19I	CCATGG	1 cut(s)	3848
BspDI	ATCGAT	2 cut(s)	1656, 4491
BspEI	TCCGGA	2 cut(s)	259, 4975
BspFNI	CGCG	8 cut(s)	58, 111, 124, 278, 293, 308, 340, 2817
BspHI	TCATGA	4 cut(s)	1162, 3753, 4657, 4684
BspMAI	CTGCAG	3 cut(s)	1104, 3716, 4010
BspMI	ACCTGC	2 cut(s)	832, 4686
BspPI	GGATC	9 cut(s)	16, 629, 1414, 1840, 2675, 3929, 4303, 4725, 4967
BspQI	GCTCTTC	2 cut(s)	2777, 5015
BspT107I	GGYRCC	3 cut(s)	4145, 4399, 4799
BspTNI	GGTCTC	2 cut(s)	257, 4704
BsrBI	CCGCTC	1 cut(s)	19
BsrDI	GCAATG	8 cut(s)	1520, 1526, 1827, 1965, 2493, 2564, 2839, 3397
BsrFI	RCCGGY	4 cut(s)	43, 440, 4148, 4584
BsrGI	TGTACA	2 cut(s)	2250, 4216
BssAI	RCCGGY	4 cut(s)	43, 440, 4148, 4584
BssNI	GRCGYC	3 cut(s)	114, 378, 4766
BssT1I	CCWWGG	2 cut(s)	2057, 3848
Bst6I	CTCTTC	6 cut(s)	578, 872, 2777, 3135, 4476, 5015
BstACI	GRCGYC	3 cut(s)	114, 378, 4766
BstAPI	GCANNNNNTGC	3 cut(s)	2154, 3859, 4895
BstAUI	TGTACA	2 cut(s)	2250, 4216
BstDSI	CCRYGG	2 cut(s)	3848, 4450
BstEII	GGTNACC	2 cut(s)	2225, 4580
BstF5I	GGATG	8 cut(s)	1622, 2715, 3109, 3118, 3427, 3625, 4446, 4930
BstFNI	CGCG	8 cut(s)	58, 111, 124, 278, 293, 308, 340, 2817
BstH2I	RGCGCY	1 cut(s)	499
BstHHI	GCGC	5 cut(s)	295, 310, 498, 2419, 4832
BstMCI	CGRYCG	7 cut(s)	48, 62, 131, 152, 200, 349, 445
BstNSI	RCATGY	6 cut(s)	2423, 2543, 3651, 4893, 4942, 5018
BstPAI	GACNNNNGTC	1 cut(s)	4599
BstPI	GGTNACC	2 cut(s)	2225, 4580
BstSFI	CTRYAG	7 cut(s)	1100, 3015, 3624, 3712, 4006, 4089, 4804
BstUI	CGCG	8 cut(s)	58, 111, 124, 278, 293, 308, 340, 2817
BstV2I	GAAGAC	1 cut(s)	4091
BstXI	CCANNNNNNTGG	4 cut(s)	973, 1612, 1954, 2222
BstZI	CGGCCG	5 cut(s)	45, 59, 128, 149, 442
Bsu15I	ATCGAT	2 cut(s)	1656, 4491
BsuI	GTATCC	2 cut(s)	2938, 4002
BsuTUI	ATCGAT	2 cut(s)	1656, 4491
BtgI	CCRYGG	2 cut(s)	3848, 4450
BtgZI	GCGATG	2 cut(s)	332, 5148
BtrI	CACGTC	2 cut(s)	4061, 4905
BtsCI	GGATG	8 cut(s)	1622, 2715, 3109, 3118, 3427, 3625, 4446, 4930
BtsI	GCAGTG	6 cut(s)	302, 3659, 3721, 3869, 4107, 4640
BveI	ACCTGC	2 cut(s)	832, 4686
CaiI	CAGNNNCTG	2 cut(s)	4129, 4409
CciI	TCATGA	4 cut(s)	1162, 3753, 4657, 4684
CciNI	GCGGCCGC	1 cut(s)	59
CfoI	GCGC	5 cut(s)	295, 310, 498, 2419, 4832
Cfr10I	RCCGGY	4 cut(s)	43, 440, 4148, 4584
Cfr13I	GGNCC	8 cut(s)	99, 187, 418, 1170, 2110, 3219, 3902, 4370
ClaI	ATCGAT	2 cut(s)	1656, 4491
CseI	GACGC	7 cut(s)	103, 267, 314, 386, 2632, 2806, 4755
CsiI	ACCWGGT	1 cut(s)	1507
CspAI	ACCGGT	1 cut(s)	4148
DraI	TTTAAA	3 cut(s)	715, 1908, 4281
DraIII	CACNNNGTG	2 cut(s)	1511, 2295
DrdI	GACNNNNNNGTC	1 cut(s)	383
DseDI	GACNNNNNNGTC	1 cut(s)	383
EaeI	YGGCCR	9 cut(s)	45, 59, 128, 149, 442, 507, 537, 2578, 5146
EagI	CGGCCG	5 cut(s)	45, 59, 128, 149, 442
Eam1104I	CTCTTC	6 cut(s)	578, 872, 2777, 3135, 4476, 5015
EarI	CTCTTC	6 cut(s)	578, 872, 2777, 3135, 4476, 5015
EciI	GGCGGA	3 cut(s)	1631, 3624, 4301
EclXI	CGGCCG	5 cut(s)	45, 59, 128, 149, 442
Eco130I	CCWWGG	2 cut(s)	2057, 3848
Eco147I	AGGCCT	2 cut(s)	547, 1225
Eco24I	GRGCYC	2 cut(s)	1842, 4389
Eco31I	GGTCTC	2 cut(s)	257, 4704
Eco32I	GATATC	1 cut(s)	4020
Eco47I	GGWCC	4 cut(s)	99, 187, 418, 1170
Eco52I	CGGCCG	5 cut(s)	45, 59, 128, 149, 442
Eco57I	CTGAAG	3 cut(s)	1962, 3320, 4818
Eco88I	CYCGRG	3 cut(s)	1841, 2274, 3983
Eco91I	GGTNACC	2 cut(s)	2225, 4580
EcoO109I	RGGNCCY	4 cut(s)	99, 187, 1170, 2110
EcoO65I	GGTNACC	2 cut(s)	2225, 4580
EcoRI	GAATTC	2 cut(s)	744, 1919
EcoRV	GATATC	1 cut(s)	4020
EcoT14I	CCWWGG	2 cut(s)	2057, 3848
EcoT22I	ATGCAT	2 cut(s)	2938, 3960
EcoT38I	GRGCYC	2 cut(s)	1842, 4389
ErhI	CCWWGG	2 cut(s)	2057, 3848
Esp3I	CGTCTC	3 cut(s)	391, 4051, 4895
FalI	AAGNNNNNCTT	2 cut(s)	3543, 3575
FaqI	GGGAC	3 cut(s)	85, 200, 1456
FauI	CCCGC	2 cut(s)	24, 5081
FauNDI	CATATG	2 cut(s)	4506, 4780
FbaI	TGATCA	5 cut(s)	216, 1200, 2503, 3328, 4710
FblI	GTMKAC	2 cut(s)	775, 2672
FokI	GGATG	8 cut(s)	1609, 2722, 3116, 3125, 3434, 3632, 4433, 4917
FriOI	GRGCYC	2 cut(s)	1842, 4389
FspAI	RTGCGCAY	1 cut(s)	2418
FspBI	CTAG	6 cut(s)	2058, 2397, 2589, 3492, 3582, 3932
FspI	TGCGCA	1 cut(s)	2418
GlaI	GCGC	5 cut(s)	294, 309, 497, 2418, 4831
GsaI	CCCAGC	1 cut(s)	5107
GsuI	CTGGAG	3 cut(s)	434, 2004, 3074
HaeII	RGCGCY	1 cut(s)	499
HgaI	GACGC	7 cut(s)	103, 267, 314, 386, 2632, 2806, 4755
HhaI	GCGC	5 cut(s)	295, 310, 498, 2419, 4832
Hin1I	GRCGYC	3 cut(s)	114, 378, 4766
Hin6I	GCGC	5 cut(s)	293, 308, 496, 2417, 4830
HinP1I	GCGC	5 cut(s)	293, 308, 496, 2417, 4830
HincII	GTYRAC	6 cut(s)	776, 1062, 2742, 3205, 3228, 4246
HindII	GTYRAC	6 cut(s)	776, 1062, 2742, 3205, 3228, 4246
HpyCH4IV	ACGT	3 cut(s)	384, 4060, 4904
HpySE526I	ACGT	3 cut(s)	384, 4060, 4904
Hsp92I	GRCGYC	3 cut(s)	114, 378, 4766
HspAI	GCGC	5 cut(s)	293, 308, 496, 2417, 4830
KflI	GGGWCCC	1 cut(s)	99
Kpn2I	TCCGGA	2 cut(s)	259, 4975
KpnI	GGTACC	1 cut(s)	4403
KroI	GCCGGC	2 cut(s)	43, 440
KroNI	GCCGGC	2 cut(s)	45, 442
Ksp22I	TGATCA	5 cut(s)	216, 1200, 2503, 3328, 4710
LguI	GCTCTTC	2 cut(s)	2777, 5015
LmnI	GCTCC	7 cut(s)	409, 1837, 1982, 3055, 4069, 4630, 4858
MabI	ACCWGGT	1 cut(s)	1507
MaeI	CTAG	6 cut(s)	2058, 2397, 2589, 3492, 3582, 3932
MaeII	ACGT	3 cut(s)	384, 4060, 4904
MbiI	CCGCTC	1 cut(s)	19
MfeI	CAATTG	7 cut(s)	887, 1291, 1515, 3402, 3815, 4737, 5046
MhlI	GDGCHC	2 cut(s)	1842, 4389
MlsI	TGGCCA	4 cut(s)	509, 539, 2580, 5148
MluNI	TGGCCA	4 cut(s)	509, 539, 2580, 5148
MmeI	TCCRAC	7 cut(s)	180, 201, 378, 660, 2390, 3301, 3445
Mox20I	TGGCCA	4 cut(s)	509, 539, 2580, 5148
Mph1103I	ATGCAT	2 cut(s)	2938, 3960
MroI	TCCGGA	2 cut(s)	259, 4975
MroNI	GCCGGC	2 cut(s)	43, 440
MroXI	GAANNNNTTC	1 cut(s)	1302
MscI	TGGCCA	4 cut(s)	509, 539, 2580, 5148
MslI	CAYNNNNRTG	6 cut(s)	1428, 1863, 1952, 1958, 2220, 2941
Msp20I	TGGCCA	4 cut(s)	509, 539, 2580, 5148
MspA1I	CMGCKG	4 cut(s)	37, 76, 148, 1259
MunI	CAATTG	7 cut(s)	887, 1291, 1515, 3402, 3815, 4737, 5046
Mva1269I	GAATGC	2 cut(s)	2419, 2975
MvnI	CGCG	8 cut(s)	58, 111, 124, 278, 293, 308, 340, 2817
NaeI	GCCGGC	2 cut(s)	45, 442
NciI	CCSGG	5 cut(s)	251, 350, 3183, 4382, 5030
NcoI	CCATGG	1 cut(s)	3848
NdeI	CATATG	2 cut(s)	4506, 4780
NgoMIV	GCCGGC	2 cut(s)	43, 440
NmuCI	GTSAC	4 cut(s)	2225, 2644, 3413, 4601
NotI	GCGGCCGC	1 cut(s)	59
NsbI	TGCGCA	1 cut(s)	2418
NsiI	ATGCAT	2 cut(s)	2938, 3960
NspI	RCATGY	6 cut(s)	2423, 2543, 3651, 4893, 4942, 5018
PaeR7I	CTCGAG	2 cut(s)	2274, 3983
PagI	TCATGA	4 cut(s)	1162, 3753, 4657, 4684
PceI	AGGCCT	2 cut(s)	547, 1225
PciI	ACATGT	2 cut(s)	4938, 5014
PciSI	GCTCTTC	2 cut(s)	2777, 5015
PcsI	WCGNNNNNNNCGW	2 cut(s)	381, 468
PctI	GAATGC	2 cut(s)	2419, 2975
PdiI	GCCGGC	2 cut(s)	45, 442
PdmI	GAANNNNTTC	1 cut(s)	1302
PflFI	GACNNNGTC	1 cut(s)	4250
PflMI	CCANNNNNTGG	2 cut(s)	505, 1532
PfoI	TCCNGGA	3 cut(s)	2705, 3594, 4855
PinAI	ACCGGT	1 cut(s)	4148
PpuMI	RGGWCCY	3 cut(s)	99, 187, 1170
PscI	ACATGT	2 cut(s)	4938, 5014
PshAI	GACNNNNGTC	1 cut(s)	4599
PshBI	ATTAAT	2 cut(s)	831, 2072
PsiI	TTATAA	3 cut(s)	1241, 1502, 3920
Psp5II	RGGWCCY	3 cut(s)	99, 187, 1170
PspEI	GGTNACC	2 cut(s)	2225, 4580
PspFI	CCCAGC	1 cut(s)	5103
PspPI	GGNCC	8 cut(s)	99, 187, 418, 1170, 2110, 3219, 3902, 4370
PspPPI	RGGWCCY	3 cut(s)	99, 187, 1170
PstI	CTGCAG	3 cut(s)	1104, 3716, 4010
PstNI	CAGNNNCTG	2 cut(s)	4129, 4409
PsyI	GACNNNGTC	1 cut(s)	4250
PvuII	CAGCTG	1 cut(s)	1259
RseI	CAYNNNNRTG	6 cut(s)	1428, 1863, 1952, 1958, 2220, 2941
SalI	GTCGAC	1 cut(s)	774
SapI	GCTCTTC	2 cut(s)	2777, 5015
Sau96I	GGNCC	8 cut(s)	99, 187, 418, 1170, 2110, 3219, 3902, 4370
ScaI	AGTACT	1 cut(s)	1106
SduI	GDGCHC	2 cut(s)	1842, 4389
SexAI	ACCWGGT	1 cut(s)	1507
SfcI	CTRYAG	7 cut(s)	1100, 3015, 3624, 3712, 4006, 4089, 4804
Sfr274I	CTCGAG	2 cut(s)	2274, 3983
SinI	GGWCC	4 cut(s)	99, 187, 418, 1170
SlaI	CTCGAG	2 cut(s)	2274, 3983
SmiMI	CAYNNNNRTG	6 cut(s)	1428, 1863, 1952, 1958, 2220, 2941
SmlI	CTYRAG	7 cut(s)	821, 1174, 2274, 3983, 4304, 4589, 5152
SmoI	CTYRAG	7 cut(s)	821, 1174, 2274, 3983, 4304, 4589, 5152
SpeI	ACTAGT	1 cut(s)	3581
SseBI	AGGCCT	2 cut(s)	547, 1225
SspI	AATATT	1 cut(s)	3478
SspMI	CTAG	6 cut(s)	2058, 2397, 2589, 3492, 3582, 3932
StuI	AGGCCT	2 cut(s)	547, 1225
StyI	CCWWGG	2 cut(s)	2057, 3848
TaiI	ACGT	3 cut(s)	387, 4063, 4907
TatI	WGTACW	7 cut(s)	881, 1104, 1178, 2250, 4158, 4216, 4953
TauI	GCSGC	8 cut(s)	61, 64, 79, 114, 151, 343, 2126, 4853
TseFI	GTSAC	4 cut(s)	2225, 2644, 3413, 4601
Tsp45I	GTSAC	4 cut(s)	2225, 2644, 3413, 4601
TspGWI	ACGGA	6 cut(s)	379, 1377, 1787, 1838, 4467, 4730
Tth111I	GACNNNGTC	1 cut(s)	4250
Van91I	CCANNNNNTGG	2 cut(s)	505, 1532
VpaK11BI	GGWCC	4 cut(s)	99, 187, 418, 1170
VspI	ATTAAT	2 cut(s)	831, 2072
XceI	RCATGY	6 cut(s)	2423, 2543, 3651, 4893, 4942, 5018
XhoI	CTCGAG	2 cut(s)	2274, 3983
XmaJI	CCTAGG	1 cut(s)	2057
XmiI	GTMKAC	2 cut(s)	775, 2672
XmnI	GAANNNNTTC	1 cut(s)	1302
XspI	CTAG	6 cut(s)	2058, 2397, 2589, 3492, 3582, 3932
ZrmI	AGTACT	1 cut(s)	1106
Zsp2I	ATGCAT	2 cut(s)	2938, 3960

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.