Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Reverse (-)

24988458 .. 24996136

Gene Structure 7679 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG245500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4008 bp

ATGAGGGGATCAAAGCCGGCCGGAATGTCGGGGCCGCAGGCTGAGCAGCGACGATTTTATCCGCAACCAGGTTCCCACCCACGCGCCGCCGTTCCGCGACGGCCGTTGCCTCGAATTCAGCGGCCGAATTTCATCGTTTATCTTCTGTCCGACAGACGGGACCACCGGCGGTCGGACATCGAGGCGGTGATCAACCAGTGCAGGGCGAAGCCGGAGAATTTCCGGGTGAGTCCGGAGAACAACACAGTCGCGTCAATGTTCTACGCGCAGTGGTACGACGCGCTCGAAGCGATGGTCTGGCTGTGGGAGTCGCGGCTCGACCGGGTTCACAATTTCATGCCCAAGCTTGACGCCAACGACTCCGTCTCCACGCCGTCCGATAGCGTGGAGCTGGAGGACCGCCTGAGAGCCTTGTTCGCCGGCCGAATTAAGCAGTTAATCGACGGCGACGAGGTGAAGAAGTGTGAAGCAAAGCGCCAAATTTTGGCCAGAGAGTACGACCGAGTTCGTAAGCTGGCCAAGAGGCCTCAGAGGAATTGGGAGGACTTACCGGAGAAGGAGAAGAGGTGCAGAGTTGAGCTTGAATTGGTTGAGAGTAGGATCAGAGAGTTCAGGTCTGCTATGAACTGCTTGCTTGCTCGTGTTGAAGGGAAAGAGTTGGAGGAGTATGGTGAAGAAGGCTTGAAGCTGTTTAAATTCGGCGAAATTTGGCATTGGAGTCGAATTCAGAGGTTAATGGTGAGGGAGTGTCGCCGACTTGATGAGGGATTGCCCATTTATGCTCATAGGCAGCAAATTCTTGAGCAGATTACTAATCAGCAGGTCATGGTGTTGATTGGAGAGACTGGTTCGGGGAAGAGTACACAATTGGTTCAGTTTCTTGCTGATTCTGGAATCGCTGCTGATAAGTCCATTGTATGCACTCAGCCTCGCAAGATTGCTGCCAATTCATTGGCAAAGAGAGTCAAAGAAGAAAGTAGTGGGTGTTTCTATGGAGAAAGCACAGTTACTAGTTATCAGACATCGTCTGGACAGCAGTTGACTTCTAAGGTAACATATATGACAGACCACTGTTTACTGCAGTACTACATGAAAGATAGAAATATGACCATGATTTCTTGCATCATAGTTGATGAGGCTCATGAGAGGACCTTGAGTACAGATCTCCTTTTAGCATTGATCAAAGATTTACTGGGTCGAAGGCATCAGTTAAGGCTTATAATAATGTCTGCAACAGCTGATGCAGAAGTGCTTTCACATTATTTTTTCAACTGTAAAATCTTTCATGTAGTGGGGAGGAACTTTCCAGTTGATGTAAGATATGCTCCCTCTTTCACTGACGGAACGGCCAGTAATGTTGCTTCTTATGTTTCTGATGTGATGAGGGTAGCAAGAGAGATCCACAAGAATGAGAAAGAAGGGACAATTCTTGCATTTTTGACTTCTCAGGTGGAAGTTGAATGGGTCTGTGAGAAGTTCATAACACCTGGTGCAATTGCATTGCCATTGCATGGGAAACTTTCTTTTGAAGAACAATCTAATGTTTTTCAAAATCACCCTGGAAAAAGAAAAATTATATTCGCCACAAATCTGGCGGAGACATCCCTGACAATTCCTGGGGTAAAGTATGTGATCGATTCTGGCATGGTCAAAGAAAGCAAATTTGAACCTGGAAGTGGTATGAATGTTCTCAGAGTTTGCAGGATCAGCCAGAGTTCTGCTAAGCAAAGAACTGGGCGTGCTGGAAGAACGGAACCTGGAATATGTTATAGACTCTACTCAGAATATGATTTTCAGGCGATGCCTCCATGTCAGGAGCCCGAGATCCGTAGAGTTCATCTTGGTGTAGCAGTTCTAAGGATTCTGGCTTTGGGTGTCAAGAATTTAAAAGAGTTTGAATTCATTGATGCACCTTGTTCTGAAGCTATAGACATGGCAATGAGGAATCTTGTTCAGTTAGGAGCTGTTAAGCAAAACAATGATGTCTATGAACTAACTCAAGAGGGTCGCGACTTGGTTAAATTGGGGGTTGAGCCTAGGCTTGGTAAATTAATTCTTGGCTGCTATCACCATAACTTGCGTAAGGAGGGCCTTGTTCTTGCGGCTGTAATGGCAAATGCAAGTAGCATATTTTGCAGAGTTGGTAATGACGAGGAGAAACTTAGGTCAGACTGCTTCAAGGTGCAATTTTGCCATCGTGATGGTGACCTCTTTACCCTTCTATCAGTGTACAAGATATGGGAATCTCTGCCTCGAGAGAGGAGAAACACATGGTGTTGGGAAAACAGCATCAATGCCAAAACTATGAGGAGATGCCAGGACTCTGTATCGGAATTGGAGTCTTGCCTTAAACATGAGCTCAATAGGATAATTCCTTGTAGCTGGCGTTGGAATGCGCATGTTTCAACTGATTGTGATATATACTTGAAAAAGGTTATACTTTCTTCCCTGGCAGAAAATGTAGCAATGTTCTCAGGATATGATCAAGTTGGTTATGAGGTGGCATTAACTGGGCAGCATGTTCGGCTGCACCCTTCTTGTTCATTGCTGGTATTCGGCCAGAAACCTAGTTGGGTTGTTTTTGGTGAACTCCTCTCAAGTTCTAATCAATATTTGGCCTGCGTCACTACCATTGATTTTAACTCTTTGTCTACCCTTGATCCCCCTCCTGTGTTTGATGTGTCCAAGATGGAAGGTCGAAAGTTGCAACTGAAAGTGTTGACTGGTTTTGGCAGTTGTTTGCTTAAAAGATTTTGTGGGAAGGGCAACAATTATCTGCATCATCTTGTATCTTGCGTCAGGACAATTTGCAATGACGAACTTATCAGCATTAAAGTCGATTATTATCAGAATGAGATTATGGTGTTTGCTACCCCACATAACATGGATAGGGTTATAAACCTTGTATCCGATGCATTGGAGTGTGAAAAAAGATGGCTGCGTAATGAATGCTTGGAGAAATGCTTGTATCATGGTTCTGGTGGCTTGCCTCCTGTAGCATTGTTTGGGGCTGGTGCAGAGATCAAGCATCTGGAGCTTCATAAAAGGTGTTTGACTGTTGATGTATTTCATTCAAAACTGGATGACATGGATGATAAGGAGTTTCTTACCGAGCTTGAAGAGTCTGCCTCTGGTAGTATCTGTGGTCATCATAAGTTCCCCGGCACTGGGCAGGAAAATGTTGACAAAGGAAAGGGGGCCAGGTTGACATTTCTTACTCCTGATGCTGCTCAGAAAGCTGTTGAATTAAATGATTCTGAGTTTAATGGTTCTATACTGAAGGTTGTTCCTTCACAGTTTGGAGGTGATCATAAGGTCTATCCACTCCTTGCTGTTAGAGCTAAAATTTTATGGCCTCGCAGGCAAAGCCACGGGTTTGCAATTGTTAAATGTGACATGGATGATATTTACCCCATGCTTGCTGATTTTTCTAACCTTGTAGTTGGAGGAAGAAATATTCGTTGTCAGCCTAGCGAAAGATACACGGACTCTGTTGTGATTAGTGGACTAAACAGAGATCTTTCTGAGAAAGAAATCCTTGATGTGTTAAGGACTGCTACTAGTAGGACAATCCTGGATTTTTTTCTGGTGAGAGGGGATGCTGTAGAAAATCCGCCATGTGGTGCATGTGAAGAAGCACTGCTAAAAGAAATATCACCCTATATGCCTAAACGGTACTCCCATAGTAGCTGCACTGTTCAGGTTTTCCAGCCTGAACCAAAGAGTCATTTCATGAGAGCTTTGATAACATTTGACGGAAGATTACACTTGGAGGCAGCAAAGGCTTTAGAACAATTGGAAGGGAAAGTATTGCCCGGCTTCCTTCCATGGCAAAAGATGGAGTGTCAGCAGTTATTTCATAGCTCTCTGTCCTGTCCTGGCCCTGTTTATCGTGTAATAAAGAAGCAGCTAGATCCTTTACTTGAAAGTTTTACACATCTAAAAGGTACTCCTTGTGATCTTGTTGCATTCTCTTTTCCAATTTATGAATTTTTTTTTTCTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1335

Amino Acids

151.66

Weight (kDa)

8.18

Isoelectric Point (pI)

45.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	262 - 416	2.1e-10	DEAD/DEAH box helicase
AAA_19	PF13245	264 - 406	2.8e-06	AAA domain
AAA_22	PF13401	272 - 409	3.9e-06	AAA domain
Helicase_C	PF00271	464 - 584	1.2e-14	Helicase conserved C-terminal domain
OB_NTP_bind	PF07717	809 - 883	4e-10	Oligonucleotide/oligosaccharide-binding (OB)-fold
RBD_DEAH11	PF24475	985 - 1099	9.5e-49	DEAH11-like, RNA-binding domain
RRM_DEAH11	PF24637	1119 - 1199	2.7e-37	DEAH11 RRM domain
KH_DEAH11_1st	PF24638	1288 - 1321	9.9e-10	DEAH11 eukaryotic type KH-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1220, 2897
Acc16I	TGCGCA	1 cut(s)	2397
Acc36I	ACCTGC	1 cut(s)	811
AccB7I	CCANNNNNTGG	2 cut(s)	484, 1511
AccI	GTMKAC	1 cut(s)	2651
AccII	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
AccIII	TCCGGA	1 cut(s)	232
AclWI	GGATC	7 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3908
AcoI	YGGCCR	8 cut(s)	18, 101, 122, 421, 486, 516, 1347, 2557
AcuI	CTGAAG	2 cut(s)	1941, 3299
AcyI	GRCGYC	1 cut(s)	351
AdeI	CACNNNGTG	2 cut(s)	1490, 2274
AfaI	GTAC	8 cut(s)	275, 497, 862, 1085, 1159, 2231, 3677, 3949
AhlI	ACTAGT	2 cut(s)	1010, 3560
Alw21I	GWGCWC	1 cut(s)	2361
Alw26I	GTCTC	3 cut(s)	370, 836, 1592
AlwI	GGATC	7 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3908
Ama87I	CYCGRG	2 cut(s)	1820, 2253
Aor13HI	TCCGGA	1 cut(s)	232
ArsI	GACNNNNNNTTYG	2 cut(s)	279, 311
AseI	ATTAAT	1 cut(s)	2051
AspA2I	CCTAGG	1 cut(s)	2036
AspLEI	GCGC	5 cut(s)	86, 268, 283, 477, 2398
AspS9I	GGNCC	7 cut(s)	32, 160, 397, 1149, 2089, 3198, 3881
AsuC2I	CCSGG	4 cut(s)	224, 323, 3162, 3816
AvaI	CYCGRG	2 cut(s)	1820, 2253
AvaII	GGWCC	3 cut(s)	160, 397, 1149
AvrII	CCTAGG	1 cut(s)	2036
BalI	TGGCCA	2 cut(s)	488, 518
BanII	GRGCYC	2 cut(s)	1821, 2361
BarI	GAAGNNNNNNTAC	2 cut(s)	1345, 1377
BauI	CACGAG	1 cut(s)	639
Bbv12I	GWGCWC	1 cut(s)	2361
BccI	CCATC	6 cut(s)	286, 2195, 2202, 2683, 2928, 3832
BceAI	ACGGC	6 cut(s)	74, 88, 116, 358, 460, 1362
BcgI	CGANNNNNNTGC	6 cut(s)	701, 735, 2504, 2538, 2818, 2852
BciVI	GTATCC	1 cut(s)	2917
BclI	TGATCA	4 cut(s)	189, 1179, 2482, 3307
BcnI	CCSGG	4 cut(s)	224, 323, 3162, 3816
BcoDI	GTCTC	3 cut(s)	370, 836, 1592
BcuI	ACTAGT	2 cut(s)	1010, 3560
BfaI	CTAG	7 cut(s)	1011, 2037, 2568, 3471, 3561, 3911, 4002
BfmI	CTRYAG	4 cut(s)	1079, 1926, 2994, 3603
BfoI	RGCGCY	1 cut(s)	478
BfuAI	ACCTGC	1 cut(s)	811
BfuI	GTATCC	1 cut(s)	2917
BglI	GCCNNNNNGGC	1 cut(s)	3361
BglII	AGATCT	2 cut(s)	1162, 3517
BlnI	CCTAGG	1 cut(s)	2036
BlpI	GCTNAGC	2 cut(s)	42, 1722
BmcAI	AGTACT	1 cut(s)	1085
Bme18I	GGWCC	3 cut(s)	160, 397, 1149
BmeT110I	CYCGRG	2 cut(s)	1820, 2253
BmgT120I	GGNCC	7 cut(s)	32, 160, 397, 1149, 2089, 3198, 3881
BmiI	GGNNCC	6 cut(s)	33, 73, 161, 1755, 1818, 3199
BmrI	ACTGGG	4 cut(s)	1202, 1743, 2520, 3177
BmuI	ACTGGG	4 cut(s)	1202, 1743, 2520, 3177
BplI	GAGNNNNNCTC	2 cut(s)	3113, 3145
BpmI	CTGGAG	2 cut(s)	413, 3053
Bpu1102I	GCTNAGC	2 cut(s)	42, 1722
BpuEI	CTTGAG	4 cut(s)	821, 1174, 1983, 2581
BpuMI	CCSGG	4 cut(s)	224, 323, 3162, 3816
Bsa29I	ATCGAT	1 cut(s)	1635
BsaBI	GATNNNNATC	1 cut(s)	2844
BsaHI	GRCGYC	1 cut(s)	351
BsaJI	CCNNGG	7 cut(s)	1558, 1616, 2036, 2448, 3160, 3370, 3827
BsaWI	WCCGGW	2 cut(s)	232, 550
BsaXI	ACNNNNNCTCC	4 cut(s)	350, 380, 551, 581
Bse118I	RCCGGY	3 cut(s)	16, 165, 419
Bse3DI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
Bse8I	GATNNNNATC	1 cut(s)	2844
BseAI	TCCGGA	1 cut(s)	232
BseCI	ATCGAT	1 cut(s)	1635
BseDI	CCNNGG	7 cut(s)	1558, 1616, 2036, 2448, 3160, 3370, 3827
BseGI	GGATG	5 cut(s)	1601, 3088, 3097, 3406, 3604
BseJI	GATNNNNATC	1 cut(s)	2844
BseMI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BseRI	GAGGAG	5 cut(s)	677, 2168, 2275, 2323, 2582
BseX3I	CGGCCG	4 cut(s)	18, 101, 122, 421
BsgI	GTGCAG	5 cut(s)	220, 589, 2513, 3036, 3676
Bsh1236I	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
Bsh1285I	CGRYCG	7 cut(s)	21, 104, 125, 173, 322, 424, 502
BshVI	ATCGAT	1 cut(s)	1635
BsiEI	CGRYCG	7 cut(s)	21, 104, 125, 173, 322, 424, 502
BsiHKAI	GWGCWC	1 cut(s)	2361
BsiHKCI	CYCGRG	2 cut(s)	1820, 2253
BslFI	GGGAC	2 cut(s)	173, 1435
BsmAI	GTCTC	3 cut(s)	370, 836, 1592
BsmBI	CGTCTC	1 cut(s)	370
BsmFI	GGGAC	2 cut(s)	173, 1435
BsmI	GAATGC	3 cut(s)	2398, 2954, 3968
BsoBI	CYCGRG	2 cut(s)	1820, 2253
Bsp1286I	GDGCHC	2 cut(s)	1821, 2361
Bsp13I	TCCGGA	1 cut(s)	232
Bsp1407I	TGTACA	1 cut(s)	2229
Bsp1720I	GCTNAGC	2 cut(s)	42, 1722
Bsp19I	CCATGG	1 cut(s)	3827
Bsp68I	TCGCGA	1 cut(s)	2010
BspDI	ATCGAT	1 cut(s)	1635
BspEI	TCCGGA	1 cut(s)	232
BspFNI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BspHI	TCATGA	2 cut(s)	1141, 3732
BspLI	GGNNCC	6 cut(s)	33, 73, 161, 1755, 1818, 3199
BspMAI	CTGCAG	1 cut(s)	1083
BspMI	ACCTGC	1 cut(s)	811
BspPI	GGATC	7 cut(s)	16, 608, 1393, 1712, 1819, 2654, 3908
BsrDI	GCAATG	6 cut(s)	1499, 1505, 1944, 2472, 2543, 2818
BsrFI	RCCGGY	3 cut(s)	16, 165, 419
BsrGI	TGTACA	1 cut(s)	2229
BssAI	RCCGGY	3 cut(s)	16, 165, 419
BssECI	CCNNGG	7 cut(s)	1558, 1616, 2036, 2448, 3160, 3370, 3827
BssNI	GRCGYC	1 cut(s)	351
BssSI	CACGAG	1 cut(s)	639
BssT1I	CCWWGG	2 cut(s)	2036, 3827
Bst2BI	CACGAG	1 cut(s)	639
Bst4CI	ACNGT	8 cut(s)	247, 1006, 1073, 1274, 3058, 3297, 3675, 3697
Bst6I	CTCTTC	3 cut(s)	557, 851, 3114
BstACI	GRCGYC	1 cut(s)	351
BstAPI	GCANNNNNTGC	1 cut(s)	2133
BstAUI	TGTACA	1 cut(s)	2229
BstDSI	CCRYGG	2 cut(s)	3370, 3827
BstEII	GGTNACC	1 cut(s)	2204
BstF5I	GGATG	5 cut(s)	1601, 3088, 3097, 3406, 3604
BstFNI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BstH2I	RGCGCY	1 cut(s)	478
BstHHI	GCGC	5 cut(s)	86, 268, 283, 477, 2398
BstMAI	GTCTC	3 cut(s)	370, 836, 1592
BstMCI	CGRYCG	7 cut(s)	21, 104, 125, 173, 322, 424, 502
BstNSI	RCATGY	3 cut(s)	2402, 2522, 3630
BstPI	GGTNACC	1 cut(s)	2204
BstSFI	CTRYAG	4 cut(s)	1079, 1926, 2994, 3603
BstUI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
BstX2I	RGATCY	5 cut(s)	1162, 1398, 1824, 3517, 3913
BstXI	CCANNNNNNTGG	3 cut(s)	952, 1591, 2201
BstYI	RGATCY	5 cut(s)	1162, 1398, 1824, 3517, 3913
BstZI	CGGCCG	4 cut(s)	18, 101, 122, 421
Bsu15I	ATCGAT	1 cut(s)	1635
BsuI	GTATCC	1 cut(s)	2917
BsuTUI	ATCGAT	1 cut(s)	1635
BtgI	CCRYGG	2 cut(s)	3370, 3827
BtgZI	GCGATG	2 cut(s)	305, 1814
BtsCI	GGATG	5 cut(s)	1601, 3088, 3097, 3406, 3604
BtsI	GCAGTG	2 cut(s)	275, 3638
BtsIMutI	CAGTG	8 cut(s)	203, 275, 1069, 1335, 2232, 3165, 3638, 3693
BtuMI	TCGCGA	1 cut(s)	2010
BveI	ACCTGC	1 cut(s)	811
CciI	TCATGA	2 cut(s)	1141, 3732
CfoI	GCGC	5 cut(s)	86, 268, 283, 477, 2398
Cfr10I	RCCGGY	3 cut(s)	16, 165, 419
Cfr13I	GGNCC	7 cut(s)	32, 160, 397, 1149, 2089, 3198, 3881
ClaI	ATCGAT	1 cut(s)	1635
CseI	GACGC	5 cut(s)	240, 287, 359, 2611, 2785
CsiI	ACCWGGT	2 cut(s)	67, 1486
Csp6I	GTAC	8 cut(s)	274, 496, 861, 1084, 1158, 2230, 3676, 3948
CviQI	GTAC	8 cut(s)	274, 496, 861, 1084, 1158, 2230, 3676, 3948
DraI	TTTAAA	2 cut(s)	694, 1887
DraIII	CACNNNGTG	2 cut(s)	1490, 2274
EaeI	YGGCCR	8 cut(s)	18, 101, 122, 421, 486, 516, 1347, 2557
EagI	CGGCCG	4 cut(s)	18, 101, 122, 421
Eam1104I	CTCTTC	3 cut(s)	557, 851, 3114
EarI	CTCTTC	3 cut(s)	557, 851, 3114
EciI	GGCGGA	2 cut(s)	1610, 3603
Ecl136II	GAGCTC	1 cut(s)	2359
EclXI	CGGCCG	4 cut(s)	18, 101, 122, 421
Eco130I	CCWWGG	2 cut(s)	2036, 3827
Eco147I	AGGCCT	1 cut(s)	526
Eco24I	GRGCYC	2 cut(s)	1821, 2361
Eco47I	GGWCC	3 cut(s)	160, 397, 1149
Eco52I	CGGCCG	4 cut(s)	18, 101, 122, 421
Eco53kI	GAGCTC	1 cut(s)	2359
Eco57I	CTGAAG	2 cut(s)	1941, 3299
Eco88I	CYCGRG	2 cut(s)	1820, 2253
Eco91I	GGTNACC	1 cut(s)	2204
EcoICRI	GAGCTC	1 cut(s)	2359
EcoO109I	RGGNCCY	2 cut(s)	1149, 2089
EcoO65I	GGTNACC	1 cut(s)	2204
EcoRI	GAATTC	3 cut(s)	114, 723, 1898
EcoT14I	CCWWGG	2 cut(s)	2036, 3827
EcoT22I	ATGCAT	1 cut(s)	2917
EcoT38I	GRGCYC	2 cut(s)	1821, 2361
ErhI	CCWWGG	2 cut(s)	2036, 3827
Esp3I	CGTCTC	1 cut(s)	370
FalI	AAGNNNNNCTT	2 cut(s)	3522, 3554
FaqI	GGGAC	2 cut(s)	173, 1435
FbaI	TGATCA	4 cut(s)	189, 1179, 2482, 3307
FblI	GTMKAC	1 cut(s)	2651
FokI	GGATG	5 cut(s)	1588, 3095, 3104, 3413, 3611
FriOI	GRGCYC	2 cut(s)	1821, 2361
FspAI	RTGCGCAY	1 cut(s)	2397
FspBI	CTAG	7 cut(s)	1011, 2037, 2568, 3471, 3561, 3911, 4002
FspI	TGCGCA	1 cut(s)	2397
GlaI	GCGC	5 cut(s)	85, 267, 282, 476, 2397
GsuI	CTGGAG	2 cut(s)	413, 3053
HaeII	RGCGCY	1 cut(s)	478
HgaI	GACGC	5 cut(s)	240, 287, 359, 2611, 2785
HhaI	GCGC	5 cut(s)	86, 268, 283, 477, 2398
Hin1I	GRCGYC	1 cut(s)	351
Hin6I	GCGC	5 cut(s)	84, 266, 281, 475, 2396
HinP1I	GCGC	5 cut(s)	84, 266, 281, 475, 2396
HincII	GTYRAC	4 cut(s)	1041, 2721, 3184, 3207
HindII	GTYRAC	4 cut(s)	1041, 2721, 3184, 3207
HindIII	AAGCTT	1 cut(s)	344
Hpy99I	CGWCG	5 cut(s)	54, 102, 281, 446, 452
HpyCH4III	ACNGT	8 cut(s)	247, 1006, 1073, 1274, 3058, 3297, 3675, 3697
Hsp92I	GRCGYC	1 cut(s)	351
HspAI	GCGC	5 cut(s)	84, 266, 281, 475, 2396
Kpn2I	TCCGGA	1 cut(s)	232
KroI	GCCGGC	2 cut(s)	16, 419
KroNI	GCCGGC	2 cut(s)	18, 421
Ksp22I	TGATCA	4 cut(s)	189, 1179, 2482, 3307
LmnI	GCTCC	5 cut(s)	388, 1330, 1816, 1961, 3034
MabI	ACCWGGT	2 cut(s)	67, 1486
MaeI	CTAG	7 cut(s)	1011, 2037, 2568, 3471, 3561, 3911, 4002
MaeIII	GTNAC	5 cut(s)	1006, 1051, 2204, 2623, 3392
MfeI	CAATTG	4 cut(s)	866, 1494, 3381, 3794
MflI	RGATCY	5 cut(s)	1162, 1398, 1824, 3517, 3913
MhlI	GDGCHC	2 cut(s)	1821, 2361
MlsI	TGGCCA	2 cut(s)	488, 518
MluNI	TGGCCA	2 cut(s)	488, 518
MmeI	TCCRAC	5 cut(s)	153, 174, 639, 2369, 3424
Mox20I	TGGCCA	2 cut(s)	488, 518
Mph1103I	ATGCAT	1 cut(s)	2917
MroI	TCCGGA	1 cut(s)	232
MroNI	GCCGGC	2 cut(s)	16, 419
MscI	TGGCCA	2 cut(s)	488, 518
MslI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
Msp20I	TGGCCA	2 cut(s)	488, 518
MspA1I	CMGCKG	2 cut(s)	121, 1238
MunI	CAATTG	4 cut(s)	866, 1494, 3381, 3794
Mva1269I	GAATGC	3 cut(s)	2398, 2954, 3968
MvnI	CGCG	7 cut(s)	84, 97, 251, 266, 281, 313, 2010
NaeI	GCCGGC	2 cut(s)	18, 421
NciI	CCSGG	4 cut(s)	224, 323, 3162, 3816
NcoI	CCATGG	1 cut(s)	3827
NgoMIV	GCCGGC	2 cut(s)	16, 419
NlaIV	GGNNCC	6 cut(s)	33, 73, 161, 1755, 1818, 3199
NmuCI	GTSAC	3 cut(s)	2204, 2623, 3392
NruI	TCGCGA	1 cut(s)	2010
NsbI	TGCGCA	1 cut(s)	2397
NsiI	ATGCAT	1 cut(s)	2917
NspI	RCATGY	3 cut(s)	2402, 2522, 3630
PaeR7I	CTCGAG	1 cut(s)	2253
PagI	TCATGA	2 cut(s)	1141, 3732
PceI	AGGCCT	1 cut(s)	526
PcsI	WCGNNNNNNNCGW	2 cut(s)	282, 447
PctI	GAATGC	3 cut(s)	2398, 2954, 3968
PdiI	GCCGGC	2 cut(s)	18, 421
PfeI	GAWTC	7 cut(s)	887, 894, 1637, 1861, 1945, 2243, 3254
PflFI	GACNNNGTC	2 cut(s)	362, 1024
PflMI	CCANNNNNTGG	2 cut(s)	484, 1511
PfoI	TCCNGGA	1 cut(s)	3573
PpuMI	RGGWCCY	1 cut(s)	1149
PshBI	ATTAAT	1 cut(s)	2051
PsiI	TTATAA	2 cut(s)	1220, 2897
Psp124BI	GAGCTC	1 cut(s)	2361
Psp5II	RGGWCCY	1 cut(s)	1149
PspEI	GGTNACC	1 cut(s)	2204
PspN4I	GGNNCC	6 cut(s)	33, 73, 161, 1755, 1818, 3199
PspPI	GGNCC	7 cut(s)	32, 160, 397, 1149, 2089, 3198, 3881
PspPPI	RGGWCCY	1 cut(s)	1149
PstI	CTGCAG	1 cut(s)	1083
PsuI	RGATCY	5 cut(s)	1162, 1398, 1824, 3517, 3913
PsyI	GACNNNGTC	2 cut(s)	362, 1024
PvuII	CAGCTG	1 cut(s)	1238
RruI	TCGCGA	1 cut(s)	2010
RsaI	GTAC	8 cut(s)	275, 497, 862, 1085, 1159, 2231, 3677, 3949
RsaNI	GTAC	8 cut(s)	274, 496, 861, 1084, 1158, 2230, 3676, 3948
RseI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
SacI	GAGCTC	1 cut(s)	2361
Sau96I	GGNCC	7 cut(s)	32, 160, 397, 1149, 2089, 3198, 3881
ScaI	AGTACT	1 cut(s)	1085
SduI	GDGCHC	2 cut(s)	1821, 2361
SexAI	ACCWGGT	2 cut(s)	67, 1486
SfcI	CTRYAG	4 cut(s)	1079, 1926, 2994, 3603
Sfr274I	CTCGAG	1 cut(s)	2253
SgrAI	CRCCGGYG	1 cut(s)	165
SinI	GGWCC	3 cut(s)	160, 397, 1149
SlaI	CTCGAG	1 cut(s)	2253
SmiMI	CAYNNNNRTG	5 cut(s)	1407, 1842, 1937, 2199, 2920
SmlI	CTYRAG	5 cut(s)	800, 1153, 1998, 2253, 2596
SmoI	CTYRAG	5 cut(s)	800, 1153, 1998, 2253, 2596
SpeI	ACTAGT	2 cut(s)	1010, 3560
SseBI	AGGCCT	1 cut(s)	526
SspI	AATATT	2 cut(s)	2612, 3457
SspMI	CTAG	7 cut(s)	1011, 2037, 2568, 3471, 3561, 3911, 4002
SstI	GAGCTC	1 cut(s)	2361
StuI	AGGCCT	1 cut(s)	526
StyI	CCWWGG	2 cut(s)	2036, 3827
TaaI	ACNGT	8 cut(s)	247, 1006, 1073, 1274, 3058, 3297, 3675, 3697
TaqII	GACCGA	1 cut(s)	516
TatI	WGTACW	4 cut(s)	860, 1083, 1157, 2229
TauI	GCSGC	5 cut(s)	37, 89, 124, 316, 2105
TfiI	GAWTC	7 cut(s)	887, 894, 1637, 1861, 1945, 2243, 3254
TscAI	CASTG	8 cut(s)	203, 275, 1076, 1342, 2232, 3172, 3645, 3700
TseFI	GTSAC	3 cut(s)	2204, 2623, 3392
Tsp45I	GTSAC	3 cut(s)	2204, 2623, 3392
TspGWI	ACGGA	6 cut(s)	352, 1356, 1766, 1817, 3500, 3771
TspRI	CASTG	8 cut(s)	203, 275, 1076, 1342, 2232, 3172, 3645, 3700
Tth111I	GACNNNGTC	2 cut(s)	362, 1024
Van91I	CCANNNNNTGG	2 cut(s)	484, 1511
VpaK11BI	GGWCC	3 cut(s)	160, 397, 1149
VspI	ATTAAT	1 cut(s)	2051
XbaI	TCTAGA	1 cut(s)	4001
XceI	RCATGY	3 cut(s)	2402, 2522, 3630
XhoI	CTCGAG	1 cut(s)	2253
XmaJI	CCTAGG	1 cut(s)	2036
XmiI	GTMKAC	1 cut(s)	2651
XspI	CTAG	7 cut(s)	1011, 2037, 2568, 3471, 3561, 3911, 4002
ZrmI	AGTACT	1 cut(s)	1085
Zsp2I	ATGCAT	1 cut(s)	2917

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.