Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0009423

Physical Location & Seq

Genomic Coordinates Forward (+)

6580699 .. 6584968

Gene Structure 4270 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g06350.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3207 bp

ATGCACAAGGATGATGTTTTTGAGTTAACTCAGGAAGGTCGGTGCTTGGTTAAGTTGGGTGTTGAACCTCGGCTTGGTAAATTAATTCTTGGTTGTTGTGACCTTAACTTGCGTATCGAGGGTCTTGTTCTTGCAGCCGTGATGGCAAATTCCAGTAGCATATTTTGTAGAGTTGGTAATGACGAAGAAAAGATTAGATCAGACTGCATTAAGGTGAAATTTTGTCATCGTGATGGTGACCTCTTTACCCTTCTCTCTGTGTACAAGGAATGGGATTCCTTGTCTCGAGACAAGAGAAACACATGGTGCTGGGAAAACAGCATCAATGCAAAAACTATGAGGAGATGCCAAGACACGGTGATGGAATTGGAATCTTGCCTTAAACATGAACTCAACATGATAATTTCTGGTACCTGGCGTTGGGATCCACATGAATCAACTGGTTGTGATAAACATTTGAAAAAGGTTATACTATCTTCCCTTGCAGATAATGTTGCAATGTTCTCAGGTTATGATCAACTTGGTTATGAAGTGGCATTAAATGGGCAGCATGTTAGACTGCATCCTTCGTGTTCATTGCTAGTATTCGGGGAGAAACCTAGTTGGGTAGTTTTTGGTGAAATTCTGTCAGTTTCTAATCAGTATTTGGTGTGCGTCACCTCCTTTGATTTCAACTCTCTGTCTACGCTTTATCCTCCTCCTTTGTTTGATGTATCTAAGATGGAAAGTCGAAAGCTTGAGCTGAAAATGTTGACTGGTTTTGGCATTACTGTCCTTAAAAGATTTTGTGGGAAGGGCAACAGTTATCTGCTTCATCTCGTGTCACGTATTAGGACAATTTGCGGGGATGAACTTATCAACATCAAAGTCGATTACTGTCAGAATGAGATTATGTTGTTTGCCACTTCACATAACATGGATAGGGTTGAAAGCTTTGTAAATGATGCCTTGGAGCGTGAACAAAAATGGATGCGTAATGAATGCTTGGAGAAATGCTTATATCACAGTTCAGGCGTGTTGCCTCCTGTAGCATTGTTTGGAGCTGGTGCTGAGATCAAACATCTGGAGCTTCAAAAAAGGTGTTTGACTTTTGATGTATTTCATTCAAAGCTGGACTCTGAAGATGATAAGGAGTTACTTAGTGAGCTTGAAAAGTCTATCTCTGGCAGTATATGTGCTATTCACAAGTTCACCAACACTGGGCAGGAAAATTTTGACAAAGGAAAGGGGGTGAGGGTAACATTTCAAACTCCTGAAGCGGCTCAAAAAGCAGCTGAGCTAAACGATTCTGAATTTAATGGCTCCATACTGAAGGTTATTCCTTCGCAGGTAGGTTTCAATGAAGGTGATCATAAAATGTTTTCATTCCCCGCTGTTAGAGCTAAGGTTTATTGGCCTCGCAGGCTAAGCAAGGGGTTTGCAATTGTTAAATGCCACATGAATGACACCCATCCCATGATTGATGACTTTTCTAACCTTGAAGTTGGAGGAAAAGTAATTCGCTGTGAGATTAGCAAAAGGGACACAGATGCTATCATGATATATGGACTAAGCAAAGATCTTTCTGAGGCTGAAATTTTAAATGTGTTGAGGACTGCTACAAGTAGGCGAATCCTTGATTTTTTCCTGCTGAGAGGAGATGCTGTAGGAAATCCGTCATGTAGTTCTTGTGAAGGAGCACTCTTAAAAGAAATATCACCCTTTATGCCTAAGAGGTATTCCCACAATAGCTGCAGTGTTCAGGTTTTCGAGCCTGAACCAAAGAATGTTTTCATGAGAGCTTTGATTACTTTTGACGGAAGATTACACTTGGAAGCGGCGAGAGCTTTAGAAGAATTAGAAGGGAAAGTACTGCCTGGGTTCCTTCCATGGCAAAAGATGAAGTGCCAACAGTTATTTCATAGCTCTCTATCCTGTCCTGCCCCTGTCTATCTTGTGATCAAGAAGCAGCTGGATGGTTTACTCTCAAGTTTTATGCATCTAAATGGTGTAGAATGCAATTTGGATAGAAATTCTAATGGTTCCTACCGAGTGAAGATATCTGCCAATGCTACAAAAATTGTTGCAGACCTGAGAAGACGTGTGGAGGAGCTTGTGAAAGGGAAGACTATCGACCACGCCAGCCTTACGCCAGCAGTTTTACAGATTCTTTTCTCCAGAGATGGCATCAGTCTAATGCATGCACTCCAGCGAGAGACGGGAACTTATATTCTTTTTGACAGACGTAGCCTCAGTGTACAGGTCTTTGGTTCTTCAGGCCAAGTTGGTGTGGTCGAGAAGAAATTGGTTGACGCCCTTTTAACCCTCCACGACAATAAGCTGCTTGAGGTCCGGCTTCAAGGTAATGCCTTGCCTCCTGAGTTAATGAAAGAGGTTGTTAACAGGTTTGGGCCAGACCTCCGGGGGCTCAAAGAGAAGGTCCCAGGGGCTGATTTTAGTCTAAATGTTCGCCGTCAAGTAATTTCCATCCATGGAAGCAAGGATGTGAAGCAGAAAGTAGAAGAGAGTATCTACGAGATTGTACAGATGTCAGGTAGTTCAACTCAGAGGTTTAAGACTGATGTTGATTGCCCCATATGTATGTGTGAGATTGAAGATGAATACCGGCTTGAGGACTGCAGTCATTTATTTTGCCGCTCGTGTTTAGTGGAGCAGTGTGAGTCAGCAATTAAAAACCAAGATAGTTTCCCAATGTTTTGTGCACACATGGGTTGCAGGTCTCTTATTTTGTTCAGTGATCTGAAATCTCTATTACCAAGTGAGAAGCTGGAGGAGCTATTTAGATCTTCATTGGGGGCATTTGTTGCGTCAAGTGGGGGCATCTACAGGTTTTGCCCGTCTCCCGACTGCTCTTCTGTGTATCAGGTAGCTGCTCCGGGAACAGACGGTGAACCATTTGTTTGTGGTGCATGTTATGCCGAGACATGTACTAGGTGCCATTTAGAACATCATCCATACCTCTCATGTGAGCAGTACCGAGAGTTCAAGGAAGACCCAGATTCATCGTTGAAGAAGTGGTGCAAAGGAAAAGAACATGTGAAGAGCTGCCCGGTTTGCAGGTCGACATTGCATCAATGGTTAGGTGTTGCTCCATTCGCGTATTCGGCAACTGCCCTTGGGACCATGGAGGAGTGCAAAGTGGGTCCTATACTCAATGGCACCACTAAATCAGAAACATATTATCCCAAAGAATCTCGTTCGAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

DEAD HA2 Helicase_C IBR OB_NTP_bind zf-C3HC4 zf-C3HC4_2 zf-RING_UBOX

Protein Analysis

1069

Amino Acids

120.19

Weight (kDa)

6.78

Isoelectric Point (pI)

46.04

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009423)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01020 AT5G10370
fragaria_vesca	FvH4_1g20200
malus_domestica	MD01G1036600.v1.1 MD15G1318100.v1.1
prunus_persica	Prupe.6G193800_v2.0.a1
pyrus_communis	pycom01g06330 pycom01g06340 pycom01g06350 pycom15g28060
rosa_chinensis	RchiOBHm_Chr2g0112281
rosa_laevigata	RLG00000017968
rosa_multiflora	Rmu_co8243893.1_g000001 Rmu_sc0004467.1_g000026 Rmu_sc0011951.1_g000004
rosa_roxburghii	Rroxscaffold_2G00131560
rosa_rugosa	Rorug02G0179800
rosa_samantha	Rh2AG232200 Rh2BG245500 Rh2CG236400 Rh2DG239900
rosa_wichuraiana	Rw2G018020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	1318, 2706, 3048
Acc65I	GGTACC	1 cut(s)	410
AccB1I	GGYRCC	3 cut(s)	410, 2934, 3158
AccBSI	CCGCTC	1 cut(s)	2637
AccI	GTMKAC	2 cut(s)	683, 3062
AccII	CGCG	1 cut(s)	3098
AciI	CCGC	5 cut(s)	843, 1259, 1371, 1817, 2635
AclWI	GGATC	2 cut(s)	419, 432
AcsI	RAATTY	7 cut(s)	148, 218, 621, 1210, 1292, 1575, 2011
AcuI	CTGAAG	4 cut(s)	1140, 1275, 1331, 2238
AcyI	GRCGYC	1 cut(s)	2292
AdeI	CACNNNGTG	1 cut(s)	306
AfaI	GTAC	7 cut(s)	263, 412, 1851, 2238, 2523, 2929, 2975
AfiI	CCNNNNNNNGG	5 cut(s)	74, 355, 420, 1200, 2611
AflIII	ACRYGT	3 cut(s)	2080, 2924, 3034
AjiI	CACGTC	1 cut(s)	2081
AjnI	CCWGG	3 cut(s)	413, 1855, 2422
AjuI	GAANNNNNNNTTGG	4 cut(s)	57, 89, 1881, 1913
Alw21I	GWGCWC	2 cut(s)	1681, 2704
Alw26I	GTCTC	6 cut(s)	282, 288, 2189, 2724, 2844, 2915
Alw44I	GTGCAC	1 cut(s)	2700
AlwI	GGATC	2 cut(s)	419, 432
AlwNI	CAGNNNCTG	1 cut(s)	2429
Ama87I	CYCGRG	1 cut(s)	285
AoxI	GGCC	3 cut(s)	1394, 2257, 2390
ApaLI	GTGCAC	1 cut(s)	2700
ApeKI	GCWGC	8 cut(s)	134, 547, 1271, 1731, 1948, 2320, 2870, 3045
ApoI	RAATTY	7 cut(s)	148, 218, 621, 1210, 1292, 1575, 2011
ArsI	GACNNNNNNTTYG	2 cut(s)	2403, 2435
AseI	ATTAAT	1 cut(s)	83
Asp700I	GAANNNNTTC	1 cut(s)	1769
Asp718I	GGTACC	1 cut(s)	410
AspS9I	GGNCC	5 cut(s)	2329, 2390, 2419, 3120, 3143
AsuC2I	CCSGG	3 cut(s)	2402, 2877, 3050
AsuII	TTCGAA	1 cut(s)	3200
AvaI	CYCGRG	1 cut(s)	285
AvaII	GGWCC	4 cut(s)	2329, 2419, 3120, 3143
BaeGI	GKGCMC	1 cut(s)	2704
BaeI	ACNNNNGTAYC	2 cut(s)	97, 130
BamHI	GGATCC	1 cut(s)	424
BanI	GGYRCC	3 cut(s)	410, 2934, 3158
BanII	GRGCYC	1 cut(s)	2409
BarI	GAAGNNNNNNTAC	2 cut(s)	1833, 1865
BauI	CACGAG	2 cut(s)	818, 2638
BbsI	GAAGAC	3 cut(s)	2083, 2111, 2997
Bbv12I	GWGCWC	2 cut(s)	1681, 2704
BbvI	GCAGC	8 cut(s)	146, 559, 1283, 1718, 1960, 2307, 2857, 3032
BccI	CCATC	8 cut(s)	136, 227, 355, 715, 1458, 1949, 2156, 2474
BceAI	ACGGC	2 cut(s)	122, 2436
BciT130I	CCWGG	3 cut(s)	415, 1857, 2424
BclI	TGATCA	3 cut(s)	514, 1348, 1938
BcnI	CCSGG	3 cut(s)	2402, 2877, 3050
BcoDI	GTCTC	6 cut(s)	282, 288, 2189, 2724, 2844, 2915
BfaI	CTAG	3 cut(s)	581, 600, 2931
BfmI	CTRYAG	5 cut(s)	1026, 1644, 1732, 2617, 2824
BfuAI	ACCTGC	3 cut(s)	1318, 2706, 3048
BglI	GCCNNNNNGGC	2 cut(s)	143, 1402
BglII	AGATCT	2 cut(s)	1558, 2783
BlpI	GCTNAGC	2 cut(s)	1275, 1406
BmcAI	AGTACT	1 cut(s)	1851
Bme1390I	CCNGG	6 cut(s)	415, 1857, 2402, 2424, 2877, 3050
Bme18I	GGWCC	4 cut(s)	2329, 2419, 3120, 3143
BmeT110I	CYCGRG	1 cut(s)	285
BmgBI	CACGTC	1 cut(s)	2081
BmgT120I	GGNCC	5 cut(s)	2329, 2390, 2419, 3120, 3143
BmrFI	CCNGG	6 cut(s)	415, 1857, 2402, 2424, 2877, 3050
BmrI	ACTGGG	1 cut(s)	1209
BmuI	ACTGGG	1 cut(s)	1209
BoxI	GACNNNNGTC	1 cut(s)	2619
BpiI	GAAGAC	3 cut(s)	2083, 2111, 2997
BpmI	CTGGAG	4 cut(s)	1085, 2140, 2171, 2789
Bpu10I	CCTNAGC	1 cut(s)	1383
Bpu1102I	GCTNAGC	2 cut(s)	1275, 1406
Bpu14I	TTCGAA	1 cut(s)	3200
BpuEI	CTTGAG	4 cut(s)	758, 1951, 2345, 2630
BpuMI	CCSGG	3 cut(s)	2402, 2877, 3050
BsaAI	YACGTR	1 cut(s)	827
BsaHI	GRCGYC	1 cut(s)	2292
BsaI	GGTCTC	1 cut(s)	2724
BsaXI	ACNNNNNCTCC	4 cut(s)	1479, 1509, 1629, 1659
Bsc4I	CCNNNNNNNGG	5 cut(s)	74, 355, 420, 1200, 2611
Bse118I	RCCGGY	1 cut(s)	2604
Bse1I	ACTGG	4 cut(s)	153, 445, 760, 1204
Bse3DI	GCAATG	3 cut(s)	504, 575, 3065
BseBI	CCWGG	3 cut(s)	415, 1857, 2424
BseGI	GGATG	9 cut(s)	16, 562, 853, 975, 1450, 1960, 2466, 2488, 2950
BseLI	CCNNNNNNNGG	5 cut(s)	74, 355, 420, 1200, 2611
BseMI	GCAATG	3 cut(s)	504, 575, 3065
BseNI	ACTGG	4 cut(s)	153, 445, 760, 1204
BseRI	GAGGAG	6 cut(s)	355, 687, 1650, 2102, 2786, 3143
BseSI	GKGCMC	1 cut(s)	2704
BseXI	GCAGC	8 cut(s)	146, 559, 1283, 1718, 1960, 2307, 2857, 3032
BseYI	CCCAGC	1 cut(s)	309
Bsh1236I	CGCG	1 cut(s)	3098
BshFI	GGCC	3 cut(s)	1396, 2259, 2392
BshNI	GGYRCC	3 cut(s)	410, 2934, 3158
BsiHKAI	GWGCWC	2 cut(s)	1681, 2704
BsiHKCI	CYCGRG	1 cut(s)	285
BsiSI	CCGG	5 cut(s)	2332, 2401, 2605, 2876, 3050
BslFI	GGGAC	3 cut(s)	1535, 2405, 3133
BslI	CCNNNNNNNGG	5 cut(s)	74, 355, 420, 1200, 2611
BsmAI	GTCTC	6 cut(s)	282, 288, 2189, 2724, 2844, 2915
BsmBI	CGTCTC	2 cut(s)	2189, 2844
BsmFI	GGGAC	3 cut(s)	1535, 2405, 3133
BsmI	GAATGC	2 cut(s)	986, 2000
BsnI	GGCC	3 cut(s)	1396, 2259, 2392
Bso31I	GGTCTC	1 cut(s)	2724
BsoBI	CYCGRG	1 cut(s)	285
Bsp119I	TTCGAA	1 cut(s)	3200
Bsp1286I	GDGCHC	3 cut(s)	1681, 2409, 2704
Bsp1407I	TGTACA	3 cut(s)	261, 2236, 2521
Bsp143I	GATC	9 cut(s)	197, 424, 514, 1053, 1348, 1558, 1938, 2737, 2783
Bsp1720I	GCTNAGC	2 cut(s)	1275, 1406
Bsp19I	CCATGG	3 cut(s)	1868, 2470, 3123
BspACI	CCGC	5 cut(s)	843, 1259, 1371, 1817, 2635
BspANI	GGCC	3 cut(s)	1396, 2259, 2392
BspFNI	CGCG	1 cut(s)	3098
BspHI	TCATGA	2 cut(s)	1536, 1773
BspMAI	CTGCAG	2 cut(s)	1736, 2621
BspMI	ACCTGC	3 cut(s)	1318, 2706, 3048
BspPI	GGATC	2 cut(s)	419, 432
BspQI	GCTCTTC	2 cut(s)	2857, 3035
BspT104I	TTCGAA	1 cut(s)	3200
BspT107I	GGYRCC	3 cut(s)	410, 2934, 3158
BspTNI	GGTCTC	1 cut(s)	2724
BsrBI	CCGCTC	1 cut(s)	2637
BsrDI	GCAATG	3 cut(s)	504, 575, 3065
BsrFI	RCCGGY	1 cut(s)	2604
BsrGI	TGTACA	3 cut(s)	261, 2236, 2521
BsrI	ACTGG	4 cut(s)	153, 445, 760, 1204
BssAI	RCCGGY	1 cut(s)	2604
BssMI	GATC	9 cut(s)	197, 424, 514, 1053, 1348, 1558, 1938, 2737, 2783
BssNI	GRCGYC	1 cut(s)	2292
BssSI	CACGAG	2 cut(s)	818, 2638
BssT1I	CCWWGG	5 cut(s)	948, 1868, 2470, 3115, 3123
Bst2BI	CACGAG	2 cut(s)	818, 2638
Bst2UI	CCWGG	3 cut(s)	415, 1857, 2424
Bst4CI	ACNGT	7 cut(s)	358, 772, 803, 878, 1007, 1893, 2888
Bst6I	CTCTTC	3 cut(s)	2496, 2857, 3035
BstACI	GRCGYC	1 cut(s)	2292
BstAPI	GCANNNNNTGC	2 cut(s)	2804, 2915
BstAUI	TGTACA	3 cut(s)	261, 2236, 2521
BstBAI	YACGTR	1 cut(s)	827
BstBI	TTCGAA	1 cut(s)	3200
BstC8I	GCNNGC	4 cut(s)	1403, 2122, 2133, 2181
BstDSI	CCRYGG	3 cut(s)	1868, 2470, 3123
BstEII	GGTNACC	1 cut(s)	236
BstF5I	GGATG	9 cut(s)	16, 562, 853, 975, 1450, 1960, 2466, 2488, 2950
BstFNI	CGCG	1 cut(s)	3098
BstKTI	GATC	9 cut(s)	200, 427, 517, 1056, 1351, 1561, 1941, 2740, 2786
BstMAI	GTCTC	6 cut(s)	282, 288, 2189, 2724, 2844, 2915
BstMBI	GATC	9 cut(s)	197, 424, 514, 1053, 1348, 1558, 1938, 2737, 2783
BstNI	CCWGG	3 cut(s)	415, 1857, 2424
BstNSI	RCATGY	5 cut(s)	554, 2183, 2913, 2928, 3038
BstPAI	GACNNNNGTC	1 cut(s)	2619
BstPI	GGTNACC	1 cut(s)	236
BstSCI	CCNGG	6 cut(s)	413, 1855, 2400, 2422, 2875, 3048
BstSFI	CTRYAG	5 cut(s)	1026, 1644, 1732, 2617, 2824
BstSLI	GKGCMC	1 cut(s)	2704
BstUI	CGCG	1 cut(s)	3098
BstV1I	GCAGC	8 cut(s)	146, 559, 1283, 1718, 1960, 2307, 2857, 3032
BstV2I	GAAGAC	3 cut(s)	2083, 2111, 2997
BstX2I	RGATCY	3 cut(s)	424, 1558, 2783
BstYI	RGATCY	3 cut(s)	424, 1558, 2783
BsuRI	GGCC	3 cut(s)	1396, 2259, 2392
BtgI	CCRYGG	3 cut(s)	1868, 2470, 3123
BtrI	CACGTC	1 cut(s)	2081
BtsCI	GGATG	9 cut(s)	16, 562, 853, 975, 1450, 1960, 2466, 2488, 2950
BtsI	GCAGTG	2 cut(s)	1741, 2660
BtsIMutI	CAGTG	5 cut(s)	1197, 1741, 2239, 2660, 2740
BveI	ACCTGC	3 cut(s)	1318, 2706, 3048
Cac8I	GCNNGC	4 cut(s)	1403, 2122, 2133, 2181
CaiI	CAGNNNCTG	1 cut(s)	2429
CciI	TCATGA	2 cut(s)	1536, 1773
Cfr10I	RCCGGY	1 cut(s)	2604
Cfr13I	GGNCC	5 cut(s)	2329, 2390, 2419, 3120, 3143
CseI	GACGC	3 cut(s)	643, 2300, 2796
Csp6I	GTAC	7 cut(s)	262, 411, 1850, 2237, 2522, 2928, 2974
CviQI	GTAC	7 cut(s)	262, 411, 1850, 2237, 2522, 2928, 2974
DpnI	GATC	9 cut(s)	199, 426, 516, 1055, 1350, 1560, 1940, 2739, 2785
DpnII	GATC	9 cut(s)	197, 424, 514, 1053, 1348, 1558, 1938, 2737, 2783
DraI	TTTAAA	1 cut(s)	1581
DraIII	CACNNNGTG	1 cut(s)	306
Eam1104I	CTCTTC	3 cut(s)	2496, 2857, 3035
EarI	CTCTTC	3 cut(s)	2496, 2857, 3035
Eco130I	CCWWGG	5 cut(s)	948, 1868, 2470, 3115, 3123
Eco24I	GRGCYC	1 cut(s)	2409
Eco31I	GGTCTC	1 cut(s)	2724
Eco32I	GATATC	1 cut(s)	2040
Eco47I	GGWCC	4 cut(s)	2329, 2419, 3120, 3143
Eco57I	CTGAAG	4 cut(s)	1140, 1275, 1331, 2238
Eco88I	CYCGRG	1 cut(s)	285
Eco91I	GGTNACC	1 cut(s)	236
EcoO109I	RGGNCCY	2 cut(s)	2419, 3143
EcoO65I	GGTNACC	1 cut(s)	236
EcoRII	CCWGG	3 cut(s)	413, 1855, 2422
EcoRV	GATATC	1 cut(s)	2040
EcoT14I	CCWWGG	5 cut(s)	948, 1868, 2470, 3115, 3123
EcoT22I	ATGCAT	2 cut(s)	1980, 2181
EcoT38I	GRGCYC	1 cut(s)	2409
ErhI	CCWWGG	5 cut(s)	948, 1868, 2470, 3115, 3123
Esp3I	CGTCTC	2 cut(s)	2189, 2844
FaqI	GGGAC	3 cut(s)	1535, 2405, 3133
FauI	CCCGC	2 cut(s)	836, 1378
FauNDI	CATATG	1 cut(s)	2576
FbaI	TGATCA	3 cut(s)	514, 1348, 1938
FblI	GTMKAC	2 cut(s)	683, 3062
FokI	GGATG	9 cut(s)	23, 549, 860, 982, 1437, 1967, 2453, 2495, 2937
FriOI	GRGCYC	1 cut(s)	2409
FspBI	CTAG	3 cut(s)	581, 600, 2931
GsaI	CCCAGC	1 cut(s)	313
GsuI	CTGGAG	4 cut(s)	1085, 2140, 2171, 2789
HaeIII	GGCC	3 cut(s)	1396, 2259, 2392
HapII	CCGG	5 cut(s)	2332, 2401, 2605, 2876, 3050
HgaI	GACGC	3 cut(s)	643, 2300, 2796
Hin1I	GRCGYC	1 cut(s)	2292
HincII	GTYRAC	5 cut(s)	27, 753, 2290, 2380, 3063
HindII	GTYRAC	5 cut(s)	27, 753, 2290, 2380, 3063
HindIII	AAGCTT	2 cut(s)	734, 931
HpaI	GTTAAC	2 cut(s)	27, 2380
HpaII	CCGG	5 cut(s)	2332, 2401, 2605, 2876, 3050
Hpy188I	TCNGA	8 cut(s)	202, 882, 1120, 1291, 1567, 2547, 2742, 3172
HpyCH4III	ACNGT	7 cut(s)	358, 772, 803, 878, 1007, 1893, 2888
HpyCH4IV	ACGT	3 cut(s)	826, 2080, 2224
HpySE526I	ACGT	3 cut(s)	826, 2080, 2224
Hsp92I	GRCGYC	1 cut(s)	2292
KpnI	GGTACC	1 cut(s)	414
Ksp22I	TGATCA	3 cut(s)	514, 1348, 1938
KspAI	GTTAAC	2 cut(s)	27, 2380
Kzo9I	GATC	9 cut(s)	197, 424, 514, 1053, 1348, 1558, 1938, 2737, 2783
LguI	GCTCTTC	2 cut(s)	2857, 3035
Lsp1109I	GCAGC	8 cut(s)	146, 559, 1283, 1718, 1960, 2307, 2857, 3032
MaeI	CTAG	3 cut(s)	581, 600, 2931
MaeII	ACGT	3 cut(s)	826, 2080, 2224
MaeIII	GTNAC	6 cut(s)	98, 236, 655, 822, 1134, 1237
MalI	GATC	9 cut(s)	199, 426, 516, 1055, 1350, 1560, 1940, 2739, 2785
MbiI	CCGCTC	1 cut(s)	2637
MboI	GATC	9 cut(s)	197, 424, 514, 1053, 1348, 1558, 1938, 2737, 2783
MfeI	CAATTG	1 cut(s)	1422
MflI	RGATCY	3 cut(s)	424, 1558, 2783
MhlI	GDGCHC	3 cut(s)	1681, 2409, 2704
MlyI	GAGTC	2 cut(s)	1109, 2669
MmeI	TCCRAC	1 cut(s)	1465
Mph1103I	ATGCAT	2 cut(s)	1980, 2181
MroXI	GAANNNNTTC	1 cut(s)	1769
MslI	CAYNNNNRTG	7 cut(s)	9, 212, 231, 359, 1440, 1983, 2579
MspA1I	CMGCKG	3 cut(s)	1274, 1373, 1951
MspI	CCGG	5 cut(s)	2332, 2401, 2605, 2876, 3050
MspR9I	CCNGG	6 cut(s)	415, 1857, 2402, 2424, 2877, 3050
MunI	CAATTG	1 cut(s)	1422
Mva1269I	GAATGC	2 cut(s)	986, 2000
MvaI	CCWGG	3 cut(s)	415, 1857, 2424
MvnI	CGCG	1 cut(s)	3098
NciI	CCSGG	3 cut(s)	2402, 2877, 3050
NcoI	CCATGG	3 cut(s)	1868, 2470, 3123
NdeI	CATATG	1 cut(s)	2576
NdeII	GATC	9 cut(s)	197, 424, 514, 1053, 1348, 1558, 1938, 2737, 2783
NmeAIII	GCCGAG	2 cut(s)	49, 2944
NmuCI	GTSAC	4 cut(s)	98, 236, 655, 822
NsiI	ATGCAT	2 cut(s)	1980, 2181
NspI	RCATGY	5 cut(s)	554, 2183, 2913, 2928, 3038
NspV	TTCGAA	1 cut(s)	3200
PaeI	GCATGC	1 cut(s)	2183
PaeR7I	CTCGAG	1 cut(s)	285
PagI	TCATGA	2 cut(s)	1536, 1773
PasI	CCCWGGG	1 cut(s)	2423
PciI	ACATGT	2 cut(s)	2924, 3034
PciSI	GCTCTTC	2 cut(s)	2857, 3035
PctI	GAATGC	2 cut(s)	986, 2000
PdmI	GAANNNNTTC	1 cut(s)	1769
PfeI	GAWTC	8 cut(s)	275, 371, 434, 1286, 1611, 2146, 2999, 3191
PfoI	TCCNGGA	1 cut(s)	2875
PleI	GAGTC	2 cut(s)	1109, 2668
PpsI	GAGTC	2 cut(s)	1109, 2668
Ppu21I	YACGTR	1 cut(s)	827
PpuMI	RGGWCCY	2 cut(s)	2419, 3143
PscI	ACATGT	2 cut(s)	2924, 3034
PshAI	GACNNNNGTC	1 cut(s)	2619
PshBI	ATTAAT	1 cut(s)	83
Psp5II	RGGWCCY	2 cut(s)	2419, 3143
Psp6I	CCWGG	3 cut(s)	413, 1855, 2422
PspEI	GGTNACC	1 cut(s)	236
PspFI	CCCAGC	1 cut(s)	309
PspGI	CCWGG	3 cut(s)	413, 1855, 2422
PspPI	GGNCC	5 cut(s)	2329, 2390, 2419, 3120, 3143
PspPPI	RGGWCCY	2 cut(s)	2419, 3143
PsrI	GAACNNNNNNTAC	2 cut(s)	2966, 2998
PstI	CTGCAG	2 cut(s)	1736, 2621
PstNI	CAGNNNCTG	1 cut(s)	2429
PsuI	RGATCY	3 cut(s)	424, 1558, 2783
PvuII	CAGCTG	2 cut(s)	1274, 1951
RsaI	GTAC	7 cut(s)	263, 412, 1851, 2238, 2523, 2929, 2975
RsaNI	GTAC	7 cut(s)	262, 411, 1850, 2237, 2522, 2928, 2974
RseI	CAYNNNNRTG	7 cut(s)	9, 212, 231, 359, 1440, 1983, 2579
SalI	GTCGAC	1 cut(s)	3061
SapI	GCTCTTC	2 cut(s)	2857, 3035
Sau3AI	GATC	9 cut(s)	197, 424, 514, 1053, 1348, 1558, 1938, 2737, 2783
Sau96I	GGNCC	5 cut(s)	2329, 2390, 2419, 3120, 3143
ScaI	AGTACT	1 cut(s)	1851
SchI	GAGTC	2 cut(s)	1109, 2669
ScrFI	CCNGG	6 cut(s)	415, 1857, 2402, 2424, 2877, 3050
SduI	GDGCHC	3 cut(s)	1681, 2409, 2704
SfcI	CTRYAG	5 cut(s)	1026, 1644, 1732, 2617, 2824
Sfr274I	CTCGAG	1 cut(s)	285
SfuI	TTCGAA	1 cut(s)	3200
SinI	GGWCC	4 cut(s)	2329, 2419, 3120, 3143
SlaI	CTCGAG	1 cut(s)	285
SmiMI	CAYNNNNRTG	7 cut(s)	9, 212, 231, 359, 1440, 1983, 2579
SmlI	CTYRAG	5 cut(s)	285, 737, 1966, 2324, 2609
SmoI	CTYRAG	5 cut(s)	285, 737, 1966, 2324, 2609
SphI	GCATGC	1 cut(s)	2183
SsiI	CCGC	5 cut(s)	843, 1259, 1371, 1817, 2635
SspMI	CTAG	3 cut(s)	581, 600, 2931
StyD4I	CCNGG	6 cut(s)	413, 1855, 2400, 2422, 2875, 3048
StyI	CCWWGG	5 cut(s)	948, 1868, 2470, 3115, 3123
TaaI	ACNGT	7 cut(s)	358, 772, 803, 878, 1007, 1893, 2888
TaiI	ACGT	3 cut(s)	829, 2083, 2227
TaqI	TCGA	9 cut(s)	117, 286, 730, 870, 1749, 2112, 2274, 3062, 3200
TatI	WGTACW	5 cut(s)	261, 1849, 2236, 2521, 2927
TauI	GCSGC	3 cut(s)	1262, 1820, 2637
TfiI	GAWTC	8 cut(s)	275, 371, 434, 1286, 1611, 2146, 2999, 3191
TscAI	CASTG	5 cut(s)	1204, 1741, 2239, 2660, 2740
TseFI	GTSAC	4 cut(s)	98, 236, 655, 822
TseI	GCWGC	8 cut(s)	134, 547, 1271, 1731, 1948, 2320, 2870, 3045
Tsp45I	GTSAC	4 cut(s)	98, 236, 655, 822
TspGWI	ACGGA	2 cut(s)	1644, 1812
TspRI	CASTG	5 cut(s)	1204, 1741, 2239, 2660, 2740
VneI	GTGCAC	1 cut(s)	2700
VpaK11BI	GGWCC	4 cut(s)	2329, 2419, 3120, 3143
VspI	ATTAAT	1 cut(s)	83
XapI	RAATTY	7 cut(s)	148, 218, 621, 1210, 1292, 1575, 2011
XceI	RCATGY	5 cut(s)	554, 2183, 2913, 2928, 3038
XhoI	CTCGAG	1 cut(s)	285
XmiI	GTMKAC	2 cut(s)	683, 3062
XmnI	GAANNNNTTC	1 cut(s)	1769
XspI	CTAG	3 cut(s)	581, 600, 2931
ZrmI	AGTACT	1 cut(s)	1851
Zsp2I	ATGCAT	2 cut(s)	1980, 2181

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.