FvH4_1g23270

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008832
Physical Location & Seq
Forward (+)
15135328 .. 15136809
1482 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23270.t1

Sequence Viewer

Length: 960 bp
ATGGAGATGCTCAAGGAAGCAGGGAAGATGATTGTAGAGGGTGCTGGAGGAGCAGTACTTGGATCGGCCTGTGAATCCTTCCTCAAATCGATAGGGAAGTGCTGCATGTTTGATTATCCCGTCCTGGAGAGCGTGAAATCCACGGTGGAGAATTTGAAGGAAATGATTCCGAAGATGGAAGATCATAACATGAAGTTGGGTGATCGTAAAGATGAAGTAAAAGAACTGAAAGAAGAGATGGAGCGGGGCACAAGGCTTCTTGACGAGTTGCCGGACTTTAATAAGTTGAGTTACAACGACCAACTTGTTGAATTGGATGCGTCTCTTAAGAGTCTGGTGAAGAAATTGCGCCTGCAGTCAGCACTTGACGTGAAGGAGGTCTTGGTTTCAACGAAGGAGAATTCTAAGGTTGTGAAGAAGAACACTAGGGTTGTCAAGTGCACCAAGAGAAAAGTTGATGCGGTGGATGAGACTGTTAAAGATATCAAGAAGATGCAGAAAGTGATCATGAACATAATGAAGAAACAAAAAGTGGCCCTTTTGGAATGCCAAGTGGAACACGCACTTGAAGGTGCGGCTCTACTAGGTAAACTACTAGAACTTGATGATGTGCTTCAAGATCCAACACTGCAAGTTCCTGTTGCGTACGCAATAATAGAATGGTTTCAGAACATCAAACGCATAACACAGCGGATGCCCCAGATATTTGAATTGGTAGAAAGGAAGACGGCAGAAAATGAAGACCTGACTTATATACCGGTTAGTGAACTAAAGGATTTCAAAATGTTGATATACTACAACACATATCACTTTTTGCAACCAACATTTTCTGACCAAACAAATTTTTGGTTGGAAATAGCACCATTTCTGACGAGTCGTCAGAAATGGTCAGAAATAAAATCTTTTGATGAAAAAATTTTCAACGAAAATCAATCTGTCGGAAAAGGGCGTCGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

320

Amino Acids

36.8

Weight (kDa)

7.54

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 15 - 138 1e-10 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 244
AciI CCGC 4 cut(s) 244, 461, 575, 691
AclWI GGATC 2 cut(s) 70, 614
AcsI RAATTY 4 cut(s) 151, 400, 841, 915
AcyI GRCGYC 1 cut(s) 949
AfaI GTAC 2 cut(s) 57, 647
AflII CTTAAG 1 cut(s) 326
AgeI ACCGGT 1 cut(s) 757
AgsI TTSAA 8 cut(s) 157, 311, 390, 569, 617, 710, 781, 922
AhdI GACNNNNNGTC 1 cut(s) 876
AjiI CACGTC 1 cut(s) 370
AjnI CCWGG 1 cut(s) 123
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 2 cut(s) 327, 464
Alw44I GTGCAC 1 cut(s) 439
AlwI GGATC 2 cut(s) 70, 614
AoxI GGCC 2 cut(s) 66, 534
ApaLI GTGCAC 1 cut(s) 439
ApeKI GCWGC 1 cut(s) 102
ApoI RAATTY 4 cut(s) 151, 400, 841, 915
AsiGI ACCGGT 1 cut(s) 757
Asp700I GAANNNNTTC 2 cut(s) 165, 663
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 1 cut(s) 535
AsuHPI GGTGA 2 cut(s) 212, 349
BaeGI GKGCMC 2 cut(s) 251, 443
BbsI GAAGAC 2 cut(s) 731, 747
Bbv12I GWGCWC 1 cut(s) 443
BbvI GCAGC 1 cut(s) 89
BccI CCATC 2 cut(s) 169, 232
BceAI ACGGC 1 cut(s) 744
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 1 cut(s) 504
BcoDI GTCTC 2 cut(s) 327, 464
BfaI CTAG 3 cut(s) 426, 584, 596
BfmI CTRYAG 1 cut(s) 353
BfrI CTTAAG 1 cut(s) 326
BisI GCNGC 2 cut(s) 103, 576
BlsI GCNGC 2 cut(s) 104, 577
BmcAI AGTACT 1 cut(s) 57
Bme1390I CCNGG 1 cut(s) 125
BmeRI GACNNNNNGTC 1 cut(s) 876
BmgBI CACGTC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 535
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 4 cut(s) 307, 448, 483, 684
BpiI GAAGAC 2 cut(s) 731, 747
BpmI CTGGAG 2 cut(s) 66, 146
Bsa29I ATCGAT 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 949
BsaJI CCNNGG 1 cut(s) 141
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bse118I RCCGGY 1 cut(s) 757
BseBI CCWGG 1 cut(s) 125
BseCI ATCGAT 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 3 cut(s) 322, 472, 699
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 2 cut(s) 251, 443
BseXI GCAGC 1 cut(s) 89
BshFI GGCC 2 cut(s) 68, 536
BshTI ACCGGT 1 cut(s) 757
BshVI ATCGAT 1 cut(s) 89
BsiHKAI GWGCWC 1 cut(s) 443
BsiSI CCGG 2 cut(s) 272, 758
BsiWI CGTACG 1 cut(s) 645
BsmAI GTCTC 2 cut(s) 327, 464
BsmBI CGTCTC 1 cut(s) 327
BsmI GAATGC 1 cut(s) 551
BsnI GGCC 2 cut(s) 68, 536
Bsp1286I GDGCHC 2 cut(s) 251, 443
Bsp143I GATC 5 cut(s) 62, 181, 202, 504, 619
BspACI CCGC 4 cut(s) 244, 461, 575, 691
BspANI GGCC 2 cut(s) 68, 536
BspDI ATCGAT 1 cut(s) 89
BspHI TCATGA 1 cut(s) 507
BspMAI CTGCAG 1 cut(s) 357
BspPI GGATC 2 cut(s) 70, 614
BspTI CTTAAG 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 757
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 5 cut(s) 62, 181, 202, 504, 619
BssNI GRCGYC 1 cut(s) 949
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 145, 475
Bst6I CTCTTC 1 cut(s) 228
BstACI GRCGYC 1 cut(s) 949
BstAFI CTTAAG 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 405
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 3 cut(s) 322, 472, 699
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 5 cut(s) 65, 184, 205, 507, 622
BstMAI GTCTC 2 cut(s) 327, 464
BstMBI GATC 5 cut(s) 62, 181, 202, 504, 619
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 353
BstSLI GKGCMC 2 cut(s) 251, 443
BstV1I GCAGC 1 cut(s) 89
BstV2I GAAGAC 2 cut(s) 731, 747
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
Bsu15I ATCGAT 1 cut(s) 89
BsuRI GGCC 2 cut(s) 68, 536
BsuTUI ATCGAT 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 141
BtrI CACGTC 1 cut(s) 370
BtsCI GGATG 3 cut(s) 322, 472, 699
BtsI GCAGTG 1 cut(s) 626
BtsIMutI CAGTG 1 cut(s) 626
Cac8I GCNNGC 1 cut(s) 353
CciI TCATGA 1 cut(s) 507
CfoI GCGC 1 cut(s) 351
Cfr10I RCCGGY 1 cut(s) 757
Cfr13I GGNCC 1 cut(s) 535
ClaI ATCGAT 1 cut(s) 89
CseI GACGC 2 cut(s) 309, 938
Csp6I GTAC 2 cut(s) 56, 646
CspAI ACCGGT 1 cut(s) 757
CviAII CATG 3 cut(s) 106, 190, 508
CviJI RGCY 4 cut(s) 68, 256, 536, 578
CviKI_1 RGCY 4 cut(s) 68, 256, 536, 578
CviQI GTAC 2 cut(s) 56, 646
DdeI CTNAG 1 cut(s) 405
DpnI GATC 5 cut(s) 64, 183, 204, 506, 621
DpnII GATC 5 cut(s) 62, 181, 202, 504, 619
DriI GACNNNNNGTC 1 cut(s) 876
Eam1104I CTCTTC 1 cut(s) 228
Eam1105I GACNNNNNGTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 228
Eco32I GATATC 1 cut(s) 484
EcoRI GAATTC 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 123
EcoRV GATATC 1 cut(s) 484
Esp3I CGTCTC 1 cut(s) 327
FaeI CATG 3 cut(s) 109, 193, 511
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 522, 554
FatI CATG 3 cut(s) 105, 189, 507
FauI CCCGC 1 cut(s) 237
FbaI TGATCA 1 cut(s) 504
Fnu4HI GCNGC 2 cut(s) 103, 576
FokI GGATG 3 cut(s) 329, 479, 706
Fsp4HI GCNGC 2 cut(s) 103, 576
FspBI CTAG 3 cut(s) 426, 584, 596
GlaI GCGC 1 cut(s) 350
GluI GCNGC 2 cut(s) 103, 576
GsuI CTGGAG 2 cut(s) 66, 146
HaeIII GGCC 2 cut(s) 68, 536
HapII CCGG 2 cut(s) 272, 758
HgaI GACGC 2 cut(s) 309, 938
HhaI GCGC 1 cut(s) 351
Hin1I GRCGYC 1 cut(s) 949
Hin1II CATG 3 cut(s) 109, 193, 511
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HinfI GANTC 4 cut(s) 74, 166, 331, 874
HpaII CCGG 2 cut(s) 272, 758
HphI GGTGA 2 cut(s) 212, 349
Hpy166II GTNNAC 3 cut(s) 441, 590, 767
Hpy188I TCNGA 8 cut(s) 171, 669, 832, 870, 882, 892, 941, 954
Hpy188III TCNNGA 4 cut(s) 260, 487, 508, 617
Hpy8I GTNNAC 3 cut(s) 441, 590, 767
Hpy99I CGWCG 1 cut(s) 954
HpyAV CCTTC 5 cut(s) 88, 151, 367, 388, 563
HpyCH4III ACNGT 2 cut(s) 145, 475
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 6 cut(s) 105, 355, 441, 496, 631, 817
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 405
HpySE526I ACGT 1 cut(s) 369
Hsp92I GRCGYC 1 cut(s) 949
Hsp92II CATG 3 cut(s) 109, 193, 511
HspAI GCGC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 504
Kzo9I GATC 5 cut(s) 62, 181, 202, 504, 619
LmnI GCTCC 2 cut(s) 50, 241
Lsp1109I GCAGC 1 cut(s) 89
LweI GCATC 4 cut(s) 307, 448, 483, 684
MaeI CTAG 3 cut(s) 426, 584, 596
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 290
MalI GATC 5 cut(s) 64, 183, 204, 506, 621
MbiI CCGCTC 1 cut(s) 244
MboI GATC 5 cut(s) 62, 181, 202, 504, 619
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 2 cut(s) 251, 443
MluCI AATT 7 cut(s) 151, 311, 344, 400, 710, 841, 915
MlyI GAGTC 2 cut(s) 340, 883
MmeI TCCRAC 4 cut(s) 647, 831, 919, 932
MnlI CCTC 4 cut(s) 31, 41, 92, 370
MroXI GAANNNNTTC 2 cut(s) 165, 663
MseI TTAA 3 cut(s) 279, 327, 477
MspA1I CMGCKG 1 cut(s) 691
MspCI CTTAAG 1 cut(s) 326
MspI CCGG 2 cut(s) 272, 758
MspR9I CCNGG 1 cut(s) 125
Mva1269I GAATGC 1 cut(s) 551
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 5 cut(s) 62, 181, 202, 504, 619
NlaIII CATG 3 cut(s) 109, 193, 511
NspI RCATGY 1 cut(s) 109
PagI TCATGA 1 cut(s) 507
PctI GAATGC 1 cut(s) 551
PdmI GAANNNNTTC 2 cut(s) 165, 663
PfeI GAWTC 2 cut(s) 74, 166
Pfl23II CGTACG 1 cut(s) 645
PfoI TCCNGGA 1 cut(s) 123
PinAI ACCGGT 1 cut(s) 757
PkrI GCNGC 2 cut(s) 104, 577
PleI GAGTC 2 cut(s) 339, 882
PpsI GAGTC 2 cut(s) 339, 882
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspLI CGTACG 1 cut(s) 645
PspPI GGNCC 1 cut(s) 535
PstI CTGCAG 1 cut(s) 357
PsuI RGATCY 1 cut(s) 619
RsaI GTAC 2 cut(s) 57, 647
RsaNI GTAC 2 cut(s) 56, 646
SaqAI TTAA 3 cut(s) 279, 327, 477
SatI GCNGC 2 cut(s) 103, 576
Sau3AI GATC 5 cut(s) 62, 181, 202, 504, 619
Sau96I GGNCC 1 cut(s) 535
ScaI AGTACT 1 cut(s) 57
SchI GAGTC 2 cut(s) 340, 883
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 2 cut(s) 251, 443
SetI ASST 6 cut(s) 372, 381, 411, 574, 589, 747
SfaNI GCATC 4 cut(s) 307, 448, 483, 684
SfcI CTRYAG 1 cut(s) 353
SmlI CTYRAG 2 cut(s) 11, 326
SmoI CTYRAG 2 cut(s) 11, 326
Sse9I AATT 7 cut(s) 151, 311, 344, 400, 710, 841, 915
SsiI CCGC 4 cut(s) 244, 461, 575, 691
SspMI CTAG 3 cut(s) 426, 584, 596
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 145, 475
TaiI ACGT 1 cut(s) 372
TaqI TCGA 1 cut(s) 89
TasI AATT 7 cut(s) 151, 311, 344, 400, 710, 841, 915
TatI WGTACW 1 cut(s) 55
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 2 cut(s) 74, 166
Tru1I TTAA 3 cut(s) 279, 327, 477
Tru9I TTAA 3 cut(s) 279, 327, 477
TscAI CASTG 1 cut(s) 633
TseI GCWGC 1 cut(s) 102
TspDTI ATGAA 6 cut(s) 206, 228, 524, 533, 753, 924
TspRI CASTG 1 cut(s) 633
Vha464I CTTAAG 1 cut(s) 326
VneI GTGCAC 1 cut(s) 439
XapI RAATTY 4 cut(s) 151, 400, 841, 915
XceI RCATGY 1 cut(s) 109
XmnI GAANNNNTTC 2 cut(s) 165, 663
XspI CTAG 3 cut(s) 426, 584, 596
ZrmI AGTACT 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.