Rw2G013450

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008832
Physical Location & Seq
Forward (+)
14413512 .. 14415200
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013450.1

Sequence Viewer

Length: 906 bp
ATGGTTAACGCAGGGGAGTTGTTTGCAGGGGCTGCTGTTGGAGGAGCATTTGGACCCGTGTTTGATGTCTTTAGGGAAGTGGTGGAGAAGACTTTCGAGTTTAATCCCACCCTCAAAAGCGTCAAATCCACAGTGGACTCTTTACAGTTATTGATCCTGCAGATAGAAGGTCATAACAACGCGTTGGGTGGTAACCGAGAAGGGGAAGTAGAAAAGCTCAAAGAAACAATGGAGGAGGGGAAAAAGCTGATTGAAGAGCTGCTGGAGGTTAATGAGTGGAGCTGCTTGAAGGCTCATTACACCGACCAACTTGTTGGGTTGGATGAGTCTCTCAAGAGACACTTGGAGATATTGCAGGTGCAGGGAGCGATTGATGTGAAGGAGGCCTTGGTTGTGGCCAAGGATACTAAAAAGGATGTGAAGGAGGGCTTGGCTTTGACAAAGGAACATACTCGGTTCCTGAAGGGTATCTCGGTCATGGCGGAGGAAAACACCAACCAACTCGTCAGATTCCAAGCGGCTCTTAAAGCAGGACTGTCGGATCTACAGAAGGGGCAGGATGCAATGATCGACATAATGATACGTAGACTGATAAATTATCGAATGGAAGACAAAGTGGGGTCGTTGATAGAAGCCGCTGCTCTAGGAGCTACACTAGAAGAATTGTATGATGTGGTTTCAAAAGATACGTTTGAAGAGCTCTTTAGGCATTACTTTGGAGTAATACGTGCACGGACAAGTAGTATATTTGAATTGATCGAAAGGAAGAGGGCAGAAAACAAAGAGATGTCGTATATTCCGACTGAGCAACTAAAGAATTTCAGGGAACTGGATCCGTATATAATGACACATCAACAACATCTGTATGAAGTTATCAACTTATCAGACCACCTTCGCTGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

301

Amino Acids

34.14

Weight (kDa)

5.28

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 141 3.8e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 1 cut(s) 346
AccI GTMKAC 1 cut(s) 586
AccII CGCG 1 cut(s) 182
AciI CCGC 3 cut(s) 482, 518, 636
AclWI GGATC 4 cut(s) 148, 549, 827, 840
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 1 cut(s) 817
AcuI CTGAAG 1 cut(s) 482
AfiI CCNNNNNNNGG 1 cut(s) 202
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 5 cut(s) 254, 289, 681, 695, 752
AjuI GAANNNNNNNTTGG 4 cut(s) 371, 403, 413, 445
AluBI AGCT 6 cut(s) 217, 247, 259, 282, 650, 700
AluI AGCT 6 cut(s) 217, 247, 259, 282, 650, 700
Alw21I GWGCWC 2 cut(s) 702, 733
Alw26I GTCTC 2 cut(s) 331, 333
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 4 cut(s) 148, 549, 827, 840
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 2 cut(s) 384, 396
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 5 cut(s) 32, 259, 282, 638, 897
ApoI RAATTY 1 cut(s) 817
Asp700I GAANNNNTTC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 53
AvaII GGWCC 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 733
BalI TGGCCA 1 cut(s) 398
BamHI GGATCC 1 cut(s) 832
BanII GRGCYC 1 cut(s) 702
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
BbsI GAAGAC 2 cut(s) 95, 615
Bbv12I GWGCWC 2 cut(s) 702, 733
BbvI GCAGC 5 cut(s) 19, 246, 269, 625, 884
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BciVI GTATCC 1 cut(s) 397
BcoDI GTCTC 2 cut(s) 331, 333
BfaI CTAG 2 cut(s) 644, 656
BfmI CTRYAG 2 cut(s) 158, 545
BfuAI ACCTGC 1 cut(s) 346
BfuI GTATCC 1 cut(s) 397
BisI GCNGC 7 cut(s) 33, 260, 283, 519, 636, 639, 898
BlsI GCNGC 7 cut(s) 34, 261, 284, 520, 637, 640, 899
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 53
BmiI GGNNCC 3 cut(s) 55, 458, 834
BmsI GCATC 1 cut(s) 550
BpiI GAAGAC 2 cut(s) 95, 615
BpmI CTGGAG 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 317
BsaAI YACGTR 2 cut(s) 584, 728
BsaJI CCNNGG 2 cut(s) 387, 399
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 834
Bse3DI GCAATG 1 cut(s) 570
BseDI CCNNGG 2 cut(s) 387, 399
BseGI GGATG 3 cut(s) 328, 421, 565
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 1 cut(s) 834
BseRI GAGGAG 2 cut(s) 57, 248
BseSI GKGCMC 1 cut(s) 733
BseXI GCAGC 5 cut(s) 19, 246, 269, 625, 884
BsgI GTGCAG 1 cut(s) 380
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 2 cut(s) 386, 398
BsiHKAI GWGCWC 2 cut(s) 702, 733
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 331, 333
BsnI GGCC 2 cut(s) 386, 398
Bsp1286I GDGCHC 2 cut(s) 702, 733
Bsp143I GATC 5 cut(s) 153, 541, 567, 756, 832
BspACI CCGC 3 cut(s) 482, 518, 636
BspANI GGCC 2 cut(s) 386, 398
BspCNI CTCAG 1 cut(s) 796
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 3 cut(s) 55, 458, 834
BspMAI CTGCAG 1 cut(s) 162
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 4 cut(s) 148, 549, 827, 840
BspQI GCTCTTC 2 cut(s) 249, 690
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 1 cut(s) 834
BssECI CCNNGG 2 cut(s) 387, 399
BssMI GATC 5 cut(s) 153, 541, 567, 756, 832
BssT1I CCWWGG 2 cut(s) 387, 399
Bst4CI ACNGT 3 cut(s) 133, 147, 537
Bst6I CTCTTC 3 cut(s) 249, 690, 761
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBAI YACGTR 2 cut(s) 584, 728
BstDEI CTNAG 1 cut(s) 804
BstEII GGTNACC 1 cut(s) 191
BstF5I GGATG 3 cut(s) 328, 421, 565
BstFNI CGCG 1 cut(s) 182
BstKTI GATC 5 cut(s) 156, 544, 570, 759, 835
BstMAI GTCTC 2 cut(s) 331, 333
BstMBI GATC 5 cut(s) 153, 541, 567, 756, 832
BstMWI GCNNNNNNNGC 4 cut(s) 32, 527, 647, 706
BstPI GGTNACC 1 cut(s) 191
BstSFI CTRYAG 2 cut(s) 158, 545
BstSLI GKGCMC 1 cut(s) 733
BstSNI TACGTA 1 cut(s) 584
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 5 cut(s) 19, 246, 269, 625, 884
BstV2I GAAGAC 2 cut(s) 95, 615
BstX2I RGATCY 2 cut(s) 541, 832
BstXI CCANNNNNNTGG 1 cut(s) 314
BstYI RGATCY 2 cut(s) 541, 832
BsuI GTATCC 1 cut(s) 397
BsuRI GGCC 2 cut(s) 386, 398
BtsCI GGATG 3 cut(s) 328, 421, 565
BtsIMutI CAGTG 1 cut(s) 138
BveI ACCTGC 1 cut(s) 346
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 1 cut(s) 53
CseI GACGC 1 cut(s) 109
CviAII CATG 1 cut(s) 478
DdeI CTNAG 1 cut(s) 804
DpnI GATC 5 cut(s) 155, 543, 569, 758, 834
DpnII GATC 5 cut(s) 153, 541, 567, 756, 832
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 3 cut(s) 249, 690, 761
EarI CTCTTC 3 cut(s) 249, 690, 761
EciI GGCGGA 1 cut(s) 497
Ecl136II GAGCTC 1 cut(s) 700
Eco105I TACGTA 1 cut(s) 584
Eco130I CCWWGG 2 cut(s) 387, 399
Eco147I AGGCCT 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 702
Eco47I GGWCC 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 700
Eco57I CTGAAG 1 cut(s) 482
Eco91I GGTNACC 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 700
EcoO65I GGTNACC 1 cut(s) 191
EcoT14I CCWWGG 2 cut(s) 387, 399
EcoT38I GRGCYC 1 cut(s) 702
ErhI CCWWGG 2 cut(s) 387, 399
FaeI CATG 1 cut(s) 481
FalI AAGNNNNNCTT 8 cut(s) 326, 358, 371, 403, 413, 445, 507, 539
FatI CATG 1 cut(s) 477
FblI GTMKAC 1 cut(s) 586
Fnu4HI GCNGC 7 cut(s) 33, 260, 283, 519, 636, 639, 898
FokI GGATG 3 cut(s) 335, 428, 572
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 7 cut(s) 33, 260, 283, 519, 636, 639, 898
FspBI CTAG 2 cut(s) 644, 656
GluI GCNGC 7 cut(s) 33, 260, 283, 519, 636, 639, 898
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 2 cut(s) 386, 398
HgaI GACGC 1 cut(s) 109
Hin1II CATG 1 cut(s) 481
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HinfI GANTC 3 cut(s) 137, 326, 510
HpaI GTTAAC 1 cut(s) 7
Hpy166II GTNNAC 4 cut(s) 7, 136, 587, 731
Hpy188I TCNGA 4 cut(s) 509, 541, 801, 886
Hpy188III TCNNGA 2 cut(s) 334, 460
Hpy8I GTNNAC 4 cut(s) 7, 136, 587, 731
HpyAV CCTTC 8 cut(s) 161, 194, 283, 373, 415, 457, 544, 902
HpyCH4III ACNGT 3 cut(s) 133, 147, 537
HpyCH4IV ACGT 3 cut(s) 583, 689, 727
HpyCH4V TGCA 7 cut(s) 26, 160, 355, 361, 563, 731, 900
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 527, 647, 706
HpyF3I CTNAG 1 cut(s) 804
HpySE526I ACGT 3 cut(s) 583, 689, 727
Hsp92II CATG 1 cut(s) 481
KspAI GTTAAC 1 cut(s) 7
Kzo9I GATC 5 cut(s) 153, 541, 567, 756, 832
LguI GCTCTTC 2 cut(s) 249, 690
LmnI GCTCC 4 cut(s) 44, 279, 365, 647
Lsp1109I GCAGC 5 cut(s) 19, 246, 269, 625, 884
LweI GCATC 1 cut(s) 550
MaeI CTAG 2 cut(s) 644, 656
MaeII ACGT 3 cut(s) 583, 689, 727
MaeIII GTNAC 1 cut(s) 191
MalI GATC 5 cut(s) 155, 543, 569, 758, 834
MboI GATC 5 cut(s) 153, 541, 567, 756, 832
MboII GAAGA 6 cut(s) 100, 266, 620, 671, 707, 778
MflI RGATCY 2 cut(s) 541, 832
MhlI GDGCHC 2 cut(s) 702, 733
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 5 cut(s) 595, 662, 752, 817, 901
MluI ACGCGT 1 cut(s) 180
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 2 cut(s) 131, 335
MmeI TCCRAC 4 cut(s) 19, 300, 519, 824
MnlI CCTC 9 cut(s) 35, 122, 226, 229, 259, 376, 418, 478, 762
Mox20I TGGCCA 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 92
MscI TGGCCA 1 cut(s) 398
MseI TTAA 4 cut(s) 6, 102, 270, 525
MslI CAYNNNNRTG 1 cut(s) 864
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 638
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 32, 527, 647, 706
NdeII GATC 5 cut(s) 153, 541, 567, 756, 832
NlaIII CATG 1 cut(s) 481
NlaIV GGNNCC 3 cut(s) 55, 458, 834
PaqCI CACCTGC 1 cut(s) 346
PceI AGGCCT 1 cut(s) 386
PciSI GCTCTTC 2 cut(s) 249, 690
PcsI WCGNNNNNNNCGW 1 cut(s) 797
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 510
PkrI GCNGC 7 cut(s) 34, 261, 284, 520, 637, 640, 899
PleI GAGTC 2 cut(s) 131, 334
PpsI GAGTC 2 cut(s) 131, 334
Ppu21I YACGTR 2 cut(s) 584, 728
Psp124BI GAGCTC 1 cut(s) 702
PspEI GGTNACC 1 cut(s) 191
PspN4I GGNNCC 3 cut(s) 55, 458, 834
PspPI GGNCC 1 cut(s) 53
PstI CTGCAG 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 2 cut(s) 541, 832
RseI CAYNNNNRTG 1 cut(s) 864
SacI GAGCTC 1 cut(s) 702
SapI GCTCTTC 2 cut(s) 249, 690
SaqAI TTAA 4 cut(s) 6, 102, 270, 525
SatI GCNGC 7 cut(s) 33, 260, 283, 519, 636, 639, 898
Sau3AI GATC 5 cut(s) 153, 541, 567, 756, 832
Sau96I GGNCC 1 cut(s) 53
SchI GAGTC 2 cut(s) 131, 335
SduI GDGCHC 2 cut(s) 702, 733
SfaNI GCATC 1 cut(s) 550
SfcI CTRYAG 2 cut(s) 158, 545
SinI GGWCC 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SnaBI TACGTA 1 cut(s) 584
Sse9I AATT 5 cut(s) 595, 662, 752, 817, 901
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 3 cut(s) 482, 518, 636
SspMI CTAG 2 cut(s) 644, 656
SstI GAGCTC 1 cut(s) 702
StuI AGGCCT 1 cut(s) 386
StyI CCWWGG 2 cut(s) 387, 399
TaaI ACNGT 3 cut(s) 133, 147, 537
TaiI ACGT 3 cut(s) 586, 692, 730
TaqI TCGA 4 cut(s) 96, 570, 601, 759
TaqII GACCGA 1 cut(s) 463
TasI AATT 5 cut(s) 595, 662, 752, 817, 901
TauI GCSGC 2 cut(s) 521, 638
TfiI GAWTC 1 cut(s) 510
Tru1I TTAA 4 cut(s) 6, 102, 270, 525
Tru9I TTAA 4 cut(s) 6, 102, 270, 525
TscAI CASTG 1 cut(s) 138
TseI GCWGC 5 cut(s) 32, 259, 282, 638, 897
TspDTI ATGAA 1 cut(s) 882
TspGWI ACGGA 2 cut(s) 748, 825
TspRI CASTG 1 cut(s) 138
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 1 cut(s) 53
XapI RAATTY 1 cut(s) 817
XmiI GTMKAC 1 cut(s) 586
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 2 cut(s) 644, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.