RLG00000017339

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008832
Physical Location & Seq
Forward (+)
15503526 .. 15504399
874 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017339

Sequence Viewer

Length: 570 bp
ATGGAGGAGGGGAAAAGGCTCATTGAAGAGCTGCTGGAGGTTAATGAGTGGAGCTGCTTGAAGGCTCATTACACCGACCAACTTGTTGAGTTGGATGAGTCTCTCAAGAGACACTTGGAGATATTGCAGGTGCAGGGAATGATTGATGTAAAGGAGGCCTTGGTTGTGGCAAAGGATACTAATAAGGATGTGAAGGAAACCTTGGTTGTGACAAAGGATACTGGTAAGGATGTGAAAGAAGGATTGGCTTTGACAAATGATATTAGTACGGATGTGAAGGGCTATAGGCAGTGGCTGGAGCGTATCTCGGTCACGTCAGAGGAAAACATCAGCCATCTTGTCAGGTTCGAAGCAGCTCTAAAAGCAGGACTGTCGGATATAAAGAAGGATCTACAGAAGGGGCGGAAGATCGACAATATGTTGAGGGAAGTTACACTACCCATTAATCTAGCAACTACCCCAGGGGCTCTATACCCACCTCCTTCATCACTAGTCACCGACGAAGCACAATTCCAATTGCCGCTTCCTTTATTACTTGAACTTGCTCCTTTCTCATCCACCTCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

190

Amino Acids

21.17

Weight (kDa)

4.93

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 1 - 65 6.1e-07 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 118
Acc36I ACCTGC 1 cut(s) 118
AciI CCGC 2 cut(s) 403, 521
AclWI GGATC 1 cut(s) 396
AfaI GTAC 1 cut(s) 268
AgsI TTSAA 3 cut(s) 26, 61, 539
AhlI ACTAGT 1 cut(s) 490
AjiI CACGTC 1 cut(s) 315
AjnI CCWGG 1 cut(s) 460
AjuI GAANNNNNNNTTGG 6 cut(s) 185, 217, 227, 259, 507, 539
AluBI AGCT 3 cut(s) 31, 54, 356
AluI AGCT 3 cut(s) 31, 54, 356
Alw26I GTCTC 2 cut(s) 103, 105
AlwI GGATC 1 cut(s) 396
AlwNI CAGNNNCTG 1 cut(s) 295
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 3 cut(s) 31, 54, 353
AseI ATTAAT 1 cut(s) 444
AsuHPI GGTGA 1 cut(s) 487
AsuII TTCGAA 1 cut(s) 348
BanII GRGCYC 1 cut(s) 469
BarI GAAGNNNNNNTAC 4 cut(s) 53, 85, 420, 452
BbvI GCAGC 3 cut(s) 18, 41, 365
BccI CCATC 1 cut(s) 342
BcgI CGANNNNNNTGC 2 cut(s) 354, 388
BciT130I CCWGG 1 cut(s) 462
BciVI GTATCC 2 cut(s) 169, 211
BcoDI GTCTC 2 cut(s) 103, 105
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 2 cut(s) 449, 491
BfmI CTRYAG 2 cut(s) 283, 392
BfuAI ACCTGC 1 cut(s) 118
BfuI GTATCC 2 cut(s) 169, 211
BisI GCNGC 4 cut(s) 32, 55, 354, 521
BlsI GCNGC 4 cut(s) 33, 56, 355, 522
Bme1390I CCNGG 1 cut(s) 462
BmgBI CACGTC 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 2 cut(s) 56, 317
Bpu14I TTCGAA 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 89
BsaJI CCNNGG 4 cut(s) 159, 201, 460, 461
Bse1I ACTGG 1 cut(s) 226
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 4 cut(s) 159, 201, 460, 461
BseGI GGATG 5 cut(s) 100, 193, 235, 277, 554
BseNI ACTGG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 18, 41, 365
BsgI GTGCAG 1 cut(s) 152
BshFI GGCC 1 cut(s) 158
BsmAI GTCTC 2 cut(s) 103, 105
BsnI GGCC 1 cut(s) 158
Bsp119I TTCGAA 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 469
Bsp143I GATC 2 cut(s) 388, 408
BspACI CCGC 2 cut(s) 403, 521
BspANI GGCC 1 cut(s) 158
BspMI ACCTGC 1 cut(s) 118
BspPI GGATC 1 cut(s) 396
BspQI GCTCTTC 1 cut(s) 21
BspT104I TTCGAA 1 cut(s) 348
BsrI ACTGG 1 cut(s) 226
BssECI CCNNGG 4 cut(s) 159, 201, 460, 461
BssMI GATC 2 cut(s) 388, 408
BssT1I CCWWGG 2 cut(s) 159, 201
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 1 cut(s) 372
Bst6I CTCTTC 1 cut(s) 21
BstBI TTCGAA 1 cut(s) 348
BstF5I GGATG 5 cut(s) 100, 193, 235, 277, 554
BstKTI GATC 2 cut(s) 391, 411
BstMAI GTCTC 2 cut(s) 103, 105
BstMBI GATC 2 cut(s) 388, 408
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BstSFI CTRYAG 2 cut(s) 283, 392
BstV1I GCAGC 3 cut(s) 18, 41, 365
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuI GTATCC 2 cut(s) 169, 211
BsuRI GGCC 1 cut(s) 158
BtrI CACGTC 1 cut(s) 315
BtsCI GGATG 5 cut(s) 100, 193, 235, 277, 554
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 1 cut(s) 296
BveI ACCTGC 1 cut(s) 118
CaiI CAGNNNCTG 1 cut(s) 295
Csp6I GTAC 1 cut(s) 267
CviQI GTAC 1 cut(s) 267
DpnI GATC 2 cut(s) 390, 410
DpnII GATC 2 cut(s) 388, 408
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
EciI GGCGGA 1 cut(s) 418
Eco130I CCWWGG 2 cut(s) 159, 201
Eco147I AGGCCT 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 460
EcoT14I CCWWGG 2 cut(s) 159, 201
EcoT38I GRGCYC 1 cut(s) 469
ErhI CCWWGG 2 cut(s) 159, 201
FaiI YATR 4 cut(s) 285, 380, 419, 472
FalI AAGNNNNNCTT 6 cut(s) 98, 130, 143, 175, 185, 217
Fnu4HI GCNGC 4 cut(s) 32, 55, 354, 521
FokI GGATG 5 cut(s) 107, 200, 242, 284, 541
FriOI GRGCYC 1 cut(s) 469
Fsp4HI GCNGC 4 cut(s) 32, 55, 354, 521
FspBI CTAG 2 cut(s) 449, 491
GluI GCNGC 4 cut(s) 32, 55, 354, 521
GsuI CTGGAG 2 cut(s) 56, 317
HaeIII GGCC 1 cut(s) 158
HinfI GANTC 1 cut(s) 98
HphI GGTGA 1 cut(s) 487
Hpy188I TCNGA 2 cut(s) 319, 376
Hpy188III TCNNGA 1 cut(s) 106
Hpy99I CGWCG 1 cut(s) 503
HpyAV CCTTC 7 cut(s) 55, 187, 233, 271, 379, 391, 492
HpyCH4III ACNGT 1 cut(s) 372
HpyCH4IV ACGT 1 cut(s) 314
HpyCH4V TGCA 2 cut(s) 127, 133
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpySE526I ACGT 1 cut(s) 314
Kzo9I GATC 2 cut(s) 388, 408
LguI GCTCTTC 1 cut(s) 21
LmnI GCTCC 3 cut(s) 51, 298, 550
LpnPI CCDG 9 cut(s) 20, 113, 119, 207, 281, 328, 351, 447, 474
Lsp1109I GCAGC 3 cut(s) 18, 41, 365
MaeI CTAG 2 cut(s) 449, 491
MaeII ACGT 1 cut(s) 314
MaeIII GTNAC 4 cut(s) 208, 310, 430, 493
MalI GATC 2 cut(s) 390, 410
MboI GATC 2 cut(s) 388, 408
MboII GAAGA 2 cut(s) 38, 418
MfeI CAATTG 1 cut(s) 515
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 469
MluCI AATT 2 cut(s) 509, 515
MlyI GAGTC 1 cut(s) 107
MmeI TCCRAC 2 cut(s) 72, 354
MnlI CCTC 5 cut(s) 31, 148, 313, 417, 489
MseI TTAA 2 cut(s) 42, 444
MspR9I CCNGG 1 cut(s) 462
MunI CAATTG 1 cut(s) 515
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeII GATC 2 cut(s) 388, 408
NmuCI GTSAC 3 cut(s) 208, 310, 493
NspV TTCGAA 1 cut(s) 348
PaqCI CACCTGC 1 cut(s) 118
PasI CCCWGGG 1 cut(s) 461
PceI AGGCCT 1 cut(s) 158
PciSI GCTCTTC 1 cut(s) 21
PkrI GCNGC 4 cut(s) 33, 56, 355, 522
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 295
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 268
RsaNI GTAC 1 cut(s) 267
SapI GCTCTTC 1 cut(s) 21
SaqAI TTAA 2 cut(s) 42, 444
SatI GCNGC 4 cut(s) 32, 55, 354, 521
Sau3AI GATC 2 cut(s) 388, 408
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 469
SfcI CTRYAG 2 cut(s) 283, 392
SfuI TTCGAA 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 2 cut(s) 509, 515
SseBI AGGCCT 1 cut(s) 158
SsiI CCGC 2 cut(s) 403, 521
SspMI CTAG 2 cut(s) 449, 491
StuI AGGCCT 1 cut(s) 158
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 2 cut(s) 159, 201
TaaI ACNGT 1 cut(s) 372
TaiI ACGT 1 cut(s) 317
TaqI TCGA 2 cut(s) 348, 411
TaqII GACCGA 1 cut(s) 298
TasI AATT 2 cut(s) 509, 515
TauI GCSGC 1 cut(s) 523
Tru1I TTAA 2 cut(s) 42, 444
Tru9I TTAA 2 cut(s) 42, 444
TscAI CASTG 1 cut(s) 296
TseFI GTSAC 3 cut(s) 208, 310, 493
TseI GCWGC 3 cut(s) 31, 54, 353
Tsp45I GTSAC 3 cut(s) 208, 310, 493
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 1 cut(s) 284
TspRI CASTG 1 cut(s) 296
VspI ATTAAT 1 cut(s) 444
XspI CTAG 2 cut(s) 449, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.