RchiOBHm_Chr2g0104661

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008832
Physical Location & Seq
Forward (+)
15960798 .. 15962369
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47892

Sequence Viewer

Length: 1206 bp
ATGATTAACGCAGGGGAGGTGTTTGCAGGGGCTGCTGCTGGAGGACCAGTTGGACCTGTGTTTGATGTCCTTAGAGAAGTGGTAGAGAAGTCTTTCGCGTTTAATCCCACCCTCGAAAGCGTCAAATCCACGGTGGATTCTTTAGCCTTATTGATCCCGCAGATAGATGATCATAACACGAGATTGGGTGGTAACCGAGCAGGGGAAGTAGAAAAGCTCAAAGAAAAAATGGAGGAGGGGAAAAGGCTCATTGAAGAGCTGCTGCAGGTTAATGAGTGGAGCTGCTTGAAGGCTCATTACACCGACCAACTTGTTGAGTTGGATGAGTCTCTCAAGAGACACTTGGGGATATTGCAGGTGCAGGGAGTGATTGATGTGAAGGAGGCCTTGGTTGTGGCAAAGGATACTAATAAGGATGTGAAGGAAACCTTGGTTGTGGCAAAGGATACTAATAAGGATGTGAAGGAAACCTTGGTTGTGACAAAGGATGCCAGTAAGGATGTGAAAGAGGGATTGGCTTTGACAAAGGATATTAGTACGGATGTGAAGGGATATAGGCAGTGGCTGGAGCGTATCTCAGTCACGTCAGAGGAAAACATCAACCATCTTGTCAGGTTCGAAGCGGCTCTTAAAGCAGGACTGTCGGATATACAGAAGGATCTACAGAAGGGGCAGGAACAGAAGATCGACAGTATGTTGAGGGAAGTTACAATGTATGGAATGGAAAACAAAGTGGGGTCGTTGATAGAAGCGGCTGCTCTAGGAGCTACAATAGAAGAATTGGATCATGCGGTTTGGCAGGAGAACAATAAACCCATTCATCTTTCCGTAATGAGTGAACGGATAAGGGGAATAGGTGAACGGACAAGGGGGATATTTGGATTGATCGAAAGGAAGAGGGCAAAAAACATAGAGATGTCGTATATCCCGACTGTGGAACTAAAGTATTTCAGAGTGCAGCTAGAGGAGGGGGTACAGCTCGTCCGCAACTGCAACAAGGTTCTTGAGTGGACCGACGACCTAAAGGCCGAGTACAACAAGAAATTCCTTCATTTGGAGAAAATTCTCGAGAGACTACACAACCGTTTAAATGGGATACACCCGTTTAAATGGAATACTGGAAAATGCGGCAGCTCATGTGGAACAACGTCGATGAGGGAATCTATTATTATTGAGGATCGATTCTGTCATTCAGACCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

401

Amino Acids

45.17

Weight (kDa)

5.87

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 141 4.1e-20 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 247 - 358 4.1e-06 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 2 cut(s) 256, 346
AccII CGCG 1 cut(s) 98
AciI CCGC 6 cut(s) 158, 623, 752, 791, 985, 1128
AclWI GGATC 4 cut(s) 148, 666, 792, 1185
AcsI RAATTY 2 cut(s) 1043, 1062
AfaI GTAC 3 cut(s) 538, 975, 1034
AfiI CCNNNNNNNGG 3 cut(s) 202, 934, 1054
AgsI TTSAA 2 cut(s) 254, 289
AjiI CACGTC 1 cut(s) 585
AjuI GAANNNNNNNTTGG 8 cut(s) 371, 403, 413, 445, 455, 487, 497, 529
AluBI AGCT 7 cut(s) 217, 259, 282, 767, 961, 979, 1134
AluI AGCT 7 cut(s) 217, 259, 282, 767, 961, 979, 1134
Alw26I GTCTC 3 cut(s) 331, 333, 1066
AlwI GGATC 4 cut(s) 148, 666, 792, 1185
AlwNI CAGNNNCTG 2 cut(s) 32, 565
Ama87I CYCGRG 1 cut(s) 1067
AoxI GGCC 2 cut(s) 384, 1026
ApeKI GCWGC 8 cut(s) 32, 35, 259, 262, 282, 755, 958, 1131
ApoI RAATTY 2 cut(s) 1043, 1062
Asp700I GAANNNNTTC 1 cut(s) 92
AspS9I GGNCC 3 cut(s) 44, 53, 1011
AsuHPI GGTGA 1 cut(s) 869
AsuII TTCGAA 1 cut(s) 618
AvaI CYCGRG 1 cut(s) 1067
AvaII GGWCC 3 cut(s) 44, 53, 1011
BaeI ACNNNNGTAYC 2 cut(s) 1088, 1121
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
BauI CACGAG 1 cut(s) 178
BbvI GCAGC 8 cut(s) 19, 22, 246, 249, 269, 742, 970, 1143
BccI CCATC 1 cut(s) 612
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BciVI GTATCC 3 cut(s) 397, 439, 1089
BclI TGATCA 1 cut(s) 169
BcoDI GTCTC 3 cut(s) 331, 333, 1066
BfaI CTAG 2 cut(s) 761, 962
BfmI CTRYAG 2 cut(s) 263, 662
BfuAI ACCTGC 2 cut(s) 256, 346
BfuI GTATCC 3 cut(s) 397, 439, 1089
Bme18I GGWCC 3 cut(s) 44, 53, 1011
BmeT110I CYCGRG 1 cut(s) 1067
BmgBI CACGTC 1 cut(s) 585
BmgT120I GGNCC 3 cut(s) 44, 53, 1011
BmsI GCATC 1 cut(s) 478
BplI GAGNNNNNCTC 2 cut(s) 560, 592
BpmI CTGGAG 2 cut(s) 60, 587
Bpu14I TTCGAA 1 cut(s) 618
BpuEI CTTGAG 2 cut(s) 317, 1025
Bsa29I ATCGAT 1 cut(s) 1180
BsaJI CCNNGG 4 cut(s) 129, 387, 429, 471
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 934, 1054
Bse1I ACTGG 3 cut(s) 47, 492, 1123
BseCI ATCGAT 1 cut(s) 1180
BseDI CCNNGG 4 cut(s) 129, 387, 429, 471
BseGI GGATG 6 cut(s) 328, 421, 463, 493, 505, 547
BseLI CCNNNNNNNGG 3 cut(s) 202, 934, 1054
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 3 cut(s) 47, 492, 1123
BseRI GAGGAG 2 cut(s) 248, 980
BseXI GCAGC 8 cut(s) 19, 22, 246, 249, 269, 742, 970, 1143
BsgI GTGCAG 2 cut(s) 380, 977
Bsh1236I CGCG 1 cut(s) 98
BshFI GGCC 2 cut(s) 386, 1028
BshVI ATCGAT 1 cut(s) 1180
BsiHKCI CYCGRG 1 cut(s) 1067
BslI CCNNNNNNNGG 3 cut(s) 202, 934, 1054
BsmAI GTCTC 3 cut(s) 331, 333, 1066
BsnI GGCC 2 cut(s) 386, 1028
BsoBI CYCGRG 1 cut(s) 1067
Bsp119I TTCGAA 1 cut(s) 618
Bsp143I GATC 7 cut(s) 153, 169, 658, 684, 784, 885, 1177
BspACI CCGC 6 cut(s) 158, 623, 752, 791, 985, 1128
BspANI GGCC 2 cut(s) 386, 1028
BspCNI CTCAG 1 cut(s) 590
BspDI ATCGAT 1 cut(s) 1180
BspFNI CGCG 1 cut(s) 98
BspMAI CTGCAG 1 cut(s) 267
BspMI ACCTGC 2 cut(s) 256, 346
BspPI GGATC 4 cut(s) 148, 666, 792, 1185
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 1 cut(s) 618
BsrI ACTGG 3 cut(s) 47, 492, 1123
BssECI CCNNGG 4 cut(s) 129, 387, 429, 471
BssMI GATC 7 cut(s) 153, 169, 658, 684, 784, 885, 1177
BssSI CACGAG 1 cut(s) 178
BssT1I CCWWGG 3 cut(s) 387, 429, 471
Bst2BI CACGAG 1 cut(s) 178
Bst4CI ACNGT 5 cut(s) 133, 642, 692, 934, 1085
Bst6I CTCTTC 2 cut(s) 249, 890
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 618
BstDEI CTNAG 2 cut(s) 71, 577
BstDSI CCRYGG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 191
BstF5I GGATG 6 cut(s) 328, 421, 463, 493, 505, 547
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 7 cut(s) 156, 172, 661, 687, 787, 888, 1180
BstMAI GTCTC 3 cut(s) 331, 333, 1066
BstMBI GATC 7 cut(s) 153, 169, 658, 684, 784, 885, 1177
BstMWI GCNNNNNNNGC 3 cut(s) 32, 632, 764
BstPI GGTNACC 1 cut(s) 191
BstSFI CTRYAG 2 cut(s) 263, 662
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 8 cut(s) 19, 22, 246, 249, 269, 742, 970, 1143
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
Bsu15I ATCGAT 1 cut(s) 1180
BsuI GTATCC 3 cut(s) 397, 439, 1089
BsuRI GGCC 2 cut(s) 386, 1028
BsuTUI ATCGAT 1 cut(s) 1180
BtgI CCRYGG 1 cut(s) 129
BtrI CACGTC 1 cut(s) 585
BtsCI GGATG 6 cut(s) 328, 421, 463, 493, 505, 547
BtsI GCAGTG 1 cut(s) 566
BtsIMutI CAGTG 1 cut(s) 566
BveI ACCTGC 2 cut(s) 256, 346
CaiI CAGNNNCTG 2 cut(s) 32, 565
Cfr13I GGNCC 3 cut(s) 44, 53, 1011
ClaI ATCGAT 1 cut(s) 1180
CseI GACGC 1 cut(s) 109
Csp6I GTAC 3 cut(s) 537, 974, 1033
CviAII CATG 4 cut(s) 788, 1137, 1199, 1203
CviQI GTAC 3 cut(s) 537, 974, 1033
DdeI CTNAG 2 cut(s) 71, 577
DpnI GATC 7 cut(s) 155, 171, 660, 686, 786, 887, 1179
DpnII GATC 7 cut(s) 153, 169, 658, 684, 784, 885, 1177
DraI TTTAAA 2 cut(s) 1089, 1108
Eam1104I CTCTTC 2 cut(s) 249, 890
EarI CTCTTC 2 cut(s) 249, 890
Eco130I CCWWGG 3 cut(s) 387, 429, 471
Eco147I AGGCCT 1 cut(s) 386
Eco47I GGWCC 3 cut(s) 44, 53, 1011
Eco88I CYCGRG 1 cut(s) 1067
Eco91I GGTNACC 1 cut(s) 191
EcoO65I GGTNACC 1 cut(s) 191
EcoT14I CCWWGG 3 cut(s) 387, 429, 471
EcoT22I ATGCAT 1 cut(s) 1204
ErhI CCWWGG 3 cut(s) 387, 429, 471
FaeI CATG 4 cut(s) 791, 1140, 1202, 1206
FatI CATG 4 cut(s) 787, 1136, 1198, 1202
FauI CCCGC 1 cut(s) 165
FbaI TGATCA 1 cut(s) 169
FokI GGATG 6 cut(s) 335, 428, 470, 500, 512, 554
FspBI CTAG 2 cut(s) 761, 962
GsuI CTGGAG 2 cut(s) 60, 587
HaeIII GGCC 2 cut(s) 386, 1028
HgaI GACGC 1 cut(s) 109
Hin1II CATG 4 cut(s) 791, 1140, 1202, 1206
HinfI GANTC 4 cut(s) 137, 326, 1160, 1182
HphI GGTGA 1 cut(s) 869
Hpy166II GTNNAC 3 cut(s) 839, 860, 1011
Hpy188I TCNGA 4 cut(s) 589, 646, 953, 1195
Hpy188III TCNNGA 5 cut(s) 334, 928, 1004, 1067, 1069
Hpy8I GTNNAC 3 cut(s) 839, 860, 1011
Hpy99I CGWCG 2 cut(s) 1019, 1153
HpyAV CCTTC 8 cut(s) 283, 373, 415, 457, 541, 649, 661, 1058
HpyCH4III ACNGT 5 cut(s) 133, 642, 692, 934, 1085
HpyCH4IV ACGT 2 cut(s) 584, 1148
HpyCH4V TGCA 7 cut(s) 26, 265, 355, 361, 958, 993, 1202
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 632, 764
HpyF3I CTNAG 2 cut(s) 71, 577
HpySE526I ACGT 2 cut(s) 584, 1148
Hsp92II CATG 4 cut(s) 791, 1140, 1202, 1206
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 7 cut(s) 153, 169, 658, 684, 784, 885, 1177
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 3 cut(s) 279, 568, 764
Lsp1109I GCAGC 8 cut(s) 19, 22, 246, 249, 269, 742, 970, 1143
LweI GCATC 1 cut(s) 478
MaeI CTAG 2 cut(s) 761, 962
MaeII ACGT 2 cut(s) 584, 1148
MaeIII GTNAC 4 cut(s) 191, 478, 580, 706
MalI GATC 7 cut(s) 155, 171, 660, 686, 786, 887, 1179
MboI GATC 7 cut(s) 153, 169, 658, 684, 784, 885, 1177
MboII GAAGA 4 cut(s) 266, 694, 788, 907
MflI RGATCY 1 cut(s) 658
MluCI AATT 3 cut(s) 779, 1043, 1062
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 3 cut(s) 31, 300, 624
Mph1103I ATGCAT 1 cut(s) 1204
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 6 cut(s) 6, 102, 270, 630, 1088, 1107
MslI CAYNNNNRTG 1 cut(s) 914
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 32, 632, 764
NdeII GATC 7 cut(s) 153, 169, 658, 684, 784, 885, 1177
NlaIII CATG 4 cut(s) 791, 1140, 1202, 1206
NmeAIII GCCGAG 1 cut(s) 1054
NmuCI GTSAC 2 cut(s) 478, 580
NsiI ATGCAT 1 cut(s) 1204
NspV TTCGAA 1 cut(s) 618
PaeR7I CTCGAG 1 cut(s) 1067
PaqCI CACCTGC 1 cut(s) 346
PceI AGGCCT 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 1 cut(s) 926
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 3 cut(s) 137, 1160, 1182
PleI GAGTC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 334
PspEI GGTNACC 1 cut(s) 191
PspPI GGNCC 3 cut(s) 44, 53, 1011
PstI CTGCAG 1 cut(s) 267
PstNI CAGNNNCTG 2 cut(s) 32, 565
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 3 cut(s) 538, 975, 1034
RsaNI GTAC 3 cut(s) 537, 974, 1033
RseI CAYNNNNRTG 1 cut(s) 914
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 6 cut(s) 6, 102, 270, 630, 1088, 1107
Sau3AI GATC 7 cut(s) 153, 169, 658, 684, 784, 885, 1177
Sau96I GGNCC 3 cut(s) 44, 53, 1011
SchI GAGTC 1 cut(s) 335
SfaNI GCATC 1 cut(s) 478
SfcI CTRYAG 2 cut(s) 263, 662
Sfr274I CTCGAG 1 cut(s) 1067
SfuI TTCGAA 1 cut(s) 618
SinI GGWCC 3 cut(s) 44, 53, 1011
SlaI CTCGAG 1 cut(s) 1067
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 3 cut(s) 332, 1004, 1067
SmoI CTYRAG 3 cut(s) 332, 1004, 1067
Sse9I AATT 3 cut(s) 779, 1043, 1062
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 6 cut(s) 158, 623, 752, 791, 985, 1128
SspMI CTAG 2 cut(s) 761, 962
StuI AGGCCT 1 cut(s) 386
StyI CCWWGG 3 cut(s) 387, 429, 471
TaaI ACNGT 5 cut(s) 133, 642, 692, 934, 1085
TaiI ACGT 2 cut(s) 587, 1151
TaqI TCGA 7 cut(s) 114, 618, 687, 888, 1068, 1151, 1180
TaqII GACCGA 1 cut(s) 1028
TasI AATT 3 cut(s) 779, 1043, 1062
TatI WGTACW 1 cut(s) 1032
TauI GCSGC 3 cut(s) 626, 755, 1131
TfiI GAWTC 3 cut(s) 137, 1160, 1182
Tru1I TTAA 6 cut(s) 6, 102, 270, 630, 1088, 1107
Tru9I TTAA 6 cut(s) 6, 102, 270, 630, 1088, 1107
TscAI CASTG 1 cut(s) 566
TseFI GTSAC 2 cut(s) 478, 580
TseI GCWGC 8 cut(s) 32, 35, 259, 262, 282, 755, 958, 1131
Tsp45I GTSAC 2 cut(s) 478, 580
TspDTI ATGAA 2 cut(s) 809, 1040
TspGWI ACGGA 4 cut(s) 554, 817, 856, 877
TspRI CASTG 1 cut(s) 566
VpaK11BI GGWCC 3 cut(s) 44, 53, 1011
XapI RAATTY 2 cut(s) 1043, 1062
XhoI CTCGAG 1 cut(s) 1067
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 2 cut(s) 761, 962
Zsp2I ATGCAT 1 cut(s) 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.