Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0008832

Physical Location & Seq

Genomic Coordinates Forward (+)

16204397 .. 16205582

Gene Structure 1186 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG177000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGGAGGAGGGGAAAAGGCTCATTGAAGAGCTGCTGCAGGTTAATGAGTGGAGCTGCTTGAAGGCTCATTACACCGACCAACTTGTTGAGTTGGATGAGTCTCTCAAGAGACACTTGGGGATATTGCAGGTGCAGGGAGTGATTGATGTGAAGGAGGCCTTGGTTGTGGCAAAGGATACTAATAAGGATGTGAAGGAAACCTTGGTTGTGGCAAAGGATACTAATAAGGATGTGAAGGAAACCTTGGTTGTGACAAAGGATGCCAGTAAGGATGTGAAAGAGGGATTGGCTTTGACAAAGGATATTAGTACGGATGTGAAGGGATATAGGCAGTGGCTGGAGCGTATCTCAGTCACGTCAGAGGAAAACATCAACCATCTTGTCAGGTTCGAAGCGGCTCTTAAAGCAGGACTGTCGGATATACAGAAGGATCTACAGAAGGGGCAGGAACAGAAGATCGACAGTATGTTGAGGGAAGTTACAATGTATGGAATGGAAAACAAAGTGGGGTCGTTGATAGAAGCGGCTGCTCTAGGAGCTACAATAGAAGAATTGGATCATGCGGTTTGGCAGGAGAACAATAAACCCATTCATCTTTCCGTAATGAGTGAACGGATAAGGGGAATAGGTGAACGGACAAGGGGGATATTTGGATTGATCGAAAGGAAGAGGGCAAAAAACATAGAGATGTCGTATATCCCGACTGTGGAACTAAAGTATTTCAGAGTGCAGCTAGAGGAGGGGGTACAGCTCGTCCGCAACTGCAACAAGGTTCTTGAGTGGACCGACGACCTAAAGGCCGAGTACAACAAGAAATTCCTTCATTTGGAGAAAATTCTCGAGAGACTACACAACCGTTTAAATGGGATACACCCGTTTAAATGGAATACTGGAAAATGCGGCAGCTCATGTGGAACAACGTCGATGAGGGAATCTATTATTATTGAGGATCGATTCTGTCATTCAGACCATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04014 map04014

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

325

Amino Acids

37.17

Weight (kDa)

6.12

Isoelectric Point (pI)

41.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	1 - 65	7.7e-06	Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8	PF05659	171 - 282	2.7e-06	Arabidopsis broad-spectrum mildew resistance protein RPW8

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008832)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g23270
rosa_chinensis	RchiOBHm_Chr2g0104661
rosa_laevigata	RLG00000017339 RLG00000018393
rosa_multiflora	Rmu_sc0001618.1_g000010 Rmu_sc0002316.1_g000024 Rmu_sc0009463.1_g000009
rosa_roxburghii	Rroxscaffold_2G00125910 Rroxscaffold_2G00138860
rosa_rugosa	Rorug02G0121700 Rorug02G0216500
rosa_samantha	Rh2AG171900 Rh2BG179400 Rh2BG285200 Rh2BG285300 Rh2BG285400 Rh2CG177000 Rh2DG178200 Rh2DG281000 Rh2DG281300 Rh4CG069800
rosa_wichuraiana	Rw2G013450 Rw2G021580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	118
Acc36I	ACCTGC	2 cut(s)	28, 118
AciI	CCGC	5 cut(s)	395, 524, 563, 757, 900
AclWI	GGATC	3 cut(s)	438, 564, 957
AcsI	RAATTY	2 cut(s)	815, 834
AfaI	GTAC	3 cut(s)	310, 747, 806
AfiI	CCNNNNNNNGG	2 cut(s)	706, 826
AgsI	TTSAA	2 cut(s)	26, 61
AjiI	CACGTC	1 cut(s)	357
AjuI	GAANNNNNNNTTGG	8 cut(s)	143, 175, 185, 217, 227, 259, 269, 301
AluBI	AGCT	6 cut(s)	31, 54, 539, 733, 751, 906
AluI	AGCT	6 cut(s)	31, 54, 539, 733, 751, 906
Alw26I	GTCTC	3 cut(s)	103, 105, 838
AlwI	GGATC	3 cut(s)	438, 564, 957
AlwNI	CAGNNNCTG	1 cut(s)	337
Ama87I	CYCGRG	1 cut(s)	839
AoxI	GGCC	2 cut(s)	156, 798
ApeKI	GCWGC	6 cut(s)	31, 34, 54, 527, 730, 903
ApoI	RAATTY	2 cut(s)	815, 834
AspS9I	GGNCC	1 cut(s)	783
AsuHPI	GGTGA	1 cut(s)	641
AsuII	TTCGAA	1 cut(s)	390
AvaI	CYCGRG	1 cut(s)	839
AvaII	GGWCC	1 cut(s)	783
BaeI	ACNNNNGTAYC	2 cut(s)	860, 893
BarI	GAAGNNNNNNTAC	2 cut(s)	53, 85
BbvI	GCAGC	6 cut(s)	18, 21, 41, 514, 742, 915
BccI	CCATC	1 cut(s)	384
BcgI	CGANNNNNNTGC	2 cut(s)	396, 430
BciVI	GTATCC	3 cut(s)	169, 211, 861
BcoDI	GTCTC	3 cut(s)	103, 105, 838
BfaI	CTAG	2 cut(s)	533, 734
BfmI	CTRYAG	2 cut(s)	35, 434
BfuAI	ACCTGC	2 cut(s)	28, 118
BfuI	GTATCC	3 cut(s)	169, 211, 861
BisI	GCNGC	9 cut(s)	32, 35, 55, 396, 525, 528, 731, 901, 904
BlsI	GCNGC	9 cut(s)	33, 36, 56, 397, 526, 529, 732, 902, 905
Bme18I	GGWCC	1 cut(s)	783
BmeT110I	CYCGRG	1 cut(s)	839
BmgBI	CACGTC	1 cut(s)	357
BmgT120I	GGNCC	1 cut(s)	783
BmsI	GCATC	1 cut(s)	250
BplI	GAGNNNNNCTC	2 cut(s)	332, 364
BpmI	CTGGAG	1 cut(s)	359
Bpu14I	TTCGAA	1 cut(s)	390
BpuEI	CTTGAG	2 cut(s)	89, 797
Bsa29I	ATCGAT	1 cut(s)	952
BsaJI	CCNNGG	3 cut(s)	159, 201, 243
Bsc4I	CCNNNNNNNGG	2 cut(s)	706, 826
Bse1I	ACTGG	2 cut(s)	264, 895
BseCI	ATCGAT	1 cut(s)	952
BseDI	CCNNGG	3 cut(s)	159, 201, 243
BseGI	GGATG	6 cut(s)	100, 193, 235, 265, 277, 319
BseLI	CCNNNNNNNGG	2 cut(s)	706, 826
BseMII	CTCAG	1 cut(s)	363
BseNI	ACTGG	2 cut(s)	264, 895
BseRI	GAGGAG	2 cut(s)	20, 752
BseXI	GCAGC	6 cut(s)	18, 21, 41, 514, 742, 915
BsgI	GTGCAG	2 cut(s)	152, 749
BshFI	GGCC	2 cut(s)	158, 800
BshVI	ATCGAT	1 cut(s)	952
BsiHKCI	CYCGRG	1 cut(s)	839
BslI	CCNNNNNNNGG	2 cut(s)	706, 826
BsmAI	GTCTC	3 cut(s)	103, 105, 838
BsnI	GGCC	2 cut(s)	158, 800
BsoBI	CYCGRG	1 cut(s)	839
Bsp119I	TTCGAA	1 cut(s)	390
Bsp143I	GATC	5 cut(s)	430, 456, 556, 657, 949
BspACI	CCGC	5 cut(s)	395, 524, 563, 757, 900
BspANI	GGCC	2 cut(s)	158, 800
BspCNI	CTCAG	1 cut(s)	362
BspDI	ATCGAT	1 cut(s)	952
BspMAI	CTGCAG	1 cut(s)	39
BspMI	ACCTGC	2 cut(s)	28, 118
BspPI	GGATC	3 cut(s)	438, 564, 957
BspQI	GCTCTTC	1 cut(s)	21
BspT104I	TTCGAA	1 cut(s)	390
BsrI	ACTGG	2 cut(s)	264, 895
BssECI	CCNNGG	3 cut(s)	159, 201, 243
BssMI	GATC	5 cut(s)	430, 456, 556, 657, 949
BssT1I	CCWWGG	3 cut(s)	159, 201, 243
Bst4CI	ACNGT	4 cut(s)	414, 464, 706, 857
Bst6I	CTCTTC	2 cut(s)	21, 662
BstBI	TTCGAA	1 cut(s)	390
BstDEI	CTNAG	1 cut(s)	349
BstF5I	GGATG	6 cut(s)	100, 193, 235, 265, 277, 319
BstKTI	GATC	5 cut(s)	433, 459, 559, 660, 952
BstMAI	GTCTC	3 cut(s)	103, 105, 838
BstMBI	GATC	5 cut(s)	430, 456, 556, 657, 949
BstMWI	GCNNNNNNNGC	2 cut(s)	404, 536
BstSFI	CTRYAG	2 cut(s)	35, 434
BstV1I	GCAGC	6 cut(s)	18, 21, 41, 514, 742, 915
BstX2I	RGATCY	1 cut(s)	430
BstYI	RGATCY	1 cut(s)	430
Bsu15I	ATCGAT	1 cut(s)	952
BsuI	GTATCC	3 cut(s)	169, 211, 861
BsuRI	GGCC	2 cut(s)	158, 800
BsuTUI	ATCGAT	1 cut(s)	952
BtrI	CACGTC	1 cut(s)	357
BtsCI	GGATG	6 cut(s)	100, 193, 235, 265, 277, 319
BtsI	GCAGTG	1 cut(s)	338
BtsIMutI	CAGTG	1 cut(s)	338
BveI	ACCTGC	2 cut(s)	28, 118
CaiI	CAGNNNCTG	1 cut(s)	337
Cfr13I	GGNCC	1 cut(s)	783
ClaI	ATCGAT	1 cut(s)	952
Csp6I	GTAC	3 cut(s)	309, 746, 805
CviAII	CATG	4 cut(s)	560, 909, 971, 975
CviQI	GTAC	3 cut(s)	309, 746, 805
DdeI	CTNAG	1 cut(s)	349
DpnI	GATC	5 cut(s)	432, 458, 558, 659, 951
DpnII	GATC	5 cut(s)	430, 456, 556, 657, 949
DraI	TTTAAA	2 cut(s)	861, 880
Eam1104I	CTCTTC	2 cut(s)	21, 662
EarI	CTCTTC	2 cut(s)	21, 662
Eco130I	CCWWGG	3 cut(s)	159, 201, 243
Eco147I	AGGCCT	1 cut(s)	158
Eco47I	GGWCC	1 cut(s)	783
Eco88I	CYCGRG	1 cut(s)	839
EcoT14I	CCWWGG	3 cut(s)	159, 201, 243
EcoT22I	ATGCAT	1 cut(s)	976
ErhI	CCWWGG	3 cut(s)	159, 201, 243
FaeI	CATG	4 cut(s)	563, 912, 974, 978
FatI	CATG	4 cut(s)	559, 908, 970, 974
Fnu4HI	GCNGC	9 cut(s)	32, 35, 55, 396, 525, 528, 731, 901, 904
FokI	GGATG	6 cut(s)	107, 200, 242, 272, 284, 326
Fsp4HI	GCNGC	9 cut(s)	32, 35, 55, 396, 525, 528, 731, 901, 904
FspBI	CTAG	2 cut(s)	533, 734
GluI	GCNGC	9 cut(s)	32, 35, 55, 396, 525, 528, 731, 901, 904
GsuI	CTGGAG	1 cut(s)	359
HaeIII	GGCC	2 cut(s)	158, 800
Hin1II	CATG	4 cut(s)	563, 912, 974, 978
HinfI	GANTC	3 cut(s)	98, 932, 954
HphI	GGTGA	1 cut(s)	641
Hpy166II	GTNNAC	3 cut(s)	611, 632, 783
Hpy188I	TCNGA	4 cut(s)	361, 418, 725, 967
Hpy188III	TCNNGA	5 cut(s)	106, 700, 776, 839, 841
Hpy8I	GTNNAC	3 cut(s)	611, 632, 783
Hpy99I	CGWCG	2 cut(s)	791, 925
HpyAV	CCTTC	8 cut(s)	55, 145, 187, 229, 313, 421, 433, 830
HpyCH4III	ACNGT	4 cut(s)	414, 464, 706, 857
HpyCH4IV	ACGT	2 cut(s)	356, 920
HpyCH4V	TGCA	6 cut(s)	37, 127, 133, 730, 765, 974
HpyF10VI	GCNNNNNNNGC	2 cut(s)	404, 536
HpyF3I	CTNAG	1 cut(s)	349
HpySE526I	ACGT	2 cut(s)	356, 920
Hsp92II	CATG	4 cut(s)	563, 912, 974, 978
Kzo9I	GATC	5 cut(s)	430, 456, 556, 657, 949
LguI	GCTCTTC	1 cut(s)	21
LmnI	GCTCC	3 cut(s)	51, 340, 536
Lsp1109I	GCAGC	6 cut(s)	18, 21, 41, 514, 742, 915
LweI	GCATC	1 cut(s)	250
MaeI	CTAG	2 cut(s)	533, 734
MaeII	ACGT	2 cut(s)	356, 920
MaeIII	GTNAC	3 cut(s)	250, 352, 478
MalI	GATC	5 cut(s)	432, 458, 558, 659, 951
MboI	GATC	5 cut(s)	430, 456, 556, 657, 949
MboII	GAAGA	4 cut(s)	38, 466, 560, 679
MflI	RGATCY	1 cut(s)	430
MluCI	AATT	3 cut(s)	551, 815, 834
MlyI	GAGTC	1 cut(s)	107
MmeI	TCCRAC	2 cut(s)	72, 396
MnlI	CCTC	9 cut(s)	148, 274, 355, 465, 663, 730, 733, 921, 940
Mph1103I	ATGCAT	1 cut(s)	976
MseI	TTAA	4 cut(s)	42, 402, 860, 879
MslI	CAYNNNNRTG	1 cut(s)	686
MwoI	GCNNNNNNNGC	2 cut(s)	404, 536
NdeII	GATC	5 cut(s)	430, 456, 556, 657, 949
NlaIII	CATG	4 cut(s)	563, 912, 974, 978
NmeAIII	GCCGAG	1 cut(s)	826
NmuCI	GTSAC	2 cut(s)	250, 352
NsiI	ATGCAT	1 cut(s)	976
NspV	TTCGAA	1 cut(s)	390
PaeR7I	CTCGAG	1 cut(s)	839
PaqCI	CACCTGC	1 cut(s)	118
PceI	AGGCCT	1 cut(s)	158
PciSI	GCTCTTC	1 cut(s)	21
PcsI	WCGNNNNNNNCGW	1 cut(s)	698
PfeI	GAWTC	2 cut(s)	932, 954
PkrI	GCNGC	9 cut(s)	33, 36, 56, 397, 526, 529, 732, 902, 905
PleI	GAGTC	1 cut(s)	106
PpsI	GAGTC	1 cut(s)	106
PspPI	GGNCC	1 cut(s)	783
PstI	CTGCAG	1 cut(s)	39
PstNI	CAGNNNCTG	1 cut(s)	337
PsuI	RGATCY	1 cut(s)	430
RsaI	GTAC	3 cut(s)	310, 747, 806
RsaNI	GTAC	3 cut(s)	309, 746, 805
RseI	CAYNNNNRTG	1 cut(s)	686
SapI	GCTCTTC	1 cut(s)	21
SaqAI	TTAA	4 cut(s)	42, 402, 860, 879
SatI	GCNGC	9 cut(s)	32, 35, 55, 396, 525, 528, 731, 901, 904
Sau3AI	GATC	5 cut(s)	430, 456, 556, 657, 949
Sau96I	GGNCC	1 cut(s)	783
SchI	GAGTC	1 cut(s)	107
SfaNI	GCATC	1 cut(s)	250
SfcI	CTRYAG	2 cut(s)	35, 434
Sfr274I	CTCGAG	1 cut(s)	839
SfuI	TTCGAA	1 cut(s)	390
SinI	GGWCC	1 cut(s)	783
SlaI	CTCGAG	1 cut(s)	839
SmiMI	CAYNNNNRTG	1 cut(s)	686
SmlI	CTYRAG	3 cut(s)	104, 776, 839
SmoI	CTYRAG	3 cut(s)	104, 776, 839
Sse9I	AATT	3 cut(s)	551, 815, 834
SseBI	AGGCCT	1 cut(s)	158
SsiI	CCGC	5 cut(s)	395, 524, 563, 757, 900
SspMI	CTAG	2 cut(s)	533, 734
StuI	AGGCCT	1 cut(s)	158
StyI	CCWWGG	3 cut(s)	159, 201, 243
TaaI	ACNGT	4 cut(s)	414, 464, 706, 857
TaiI	ACGT	2 cut(s)	359, 923
TaqI	TCGA	6 cut(s)	390, 459, 660, 840, 923, 952
TaqII	GACCGA	1 cut(s)	800
TasI	AATT	3 cut(s)	551, 815, 834
TatI	WGTACW	1 cut(s)	804
TauI	GCSGC	3 cut(s)	398, 527, 903
TfiI	GAWTC	2 cut(s)	932, 954
Tru1I	TTAA	4 cut(s)	42, 402, 860, 879
Tru9I	TTAA	4 cut(s)	42, 402, 860, 879
TscAI	CASTG	1 cut(s)	338
TseFI	GTSAC	2 cut(s)	250, 352
TseI	GCWGC	6 cut(s)	31, 34, 54, 527, 730, 903
Tsp45I	GTSAC	2 cut(s)	250, 352
TspDTI	ATGAA	2 cut(s)	581, 812
TspGWI	ACGGA	4 cut(s)	326, 589, 628, 649
TspRI	CASTG	1 cut(s)	338
VpaK11BI	GGWCC	1 cut(s)	783
XapI	RAATTY	2 cut(s)	815, 834
XhoI	CTCGAG	1 cut(s)	839
XspI	CTAG	2 cut(s)	533, 734
Zsp2I	ATGCAT	1 cut(s)	976

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.