Rh2BG285300

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008832
Physical Location & Seq
Forward (+)
31347046 .. 31347642
597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG285300.1

Sequence Viewer

Length: 597 bp
ATGGAAATGATTAAGGAAGCAGGGGTAATGTTTGGAGAGGCTACTGCAGCAGCAGTACTTGGACCGCCCTGTGAAGGCGTCCTGGAATCGATAAAGAAGTGTTGGATGTTTGATAGTCCCGTCCTGGATAGCGTCAAATCCACGGTGGAGTACTTAGAGTCATTGATTCCGCAGATGGAAGATCATAACATGAAGTTGGCTGGCCGTCAAGATGAACTCAAAGAACTCAAAGAACAGATGGAGCGTGGCAGAAAGCTTCTTGAGGAGTTGTCGGAGGTTAATGTCCTTAGCTGCTTCAAGACAAGTTACAACCACCAACTTGTTGCATTGGATGCGTCTCTTAAGAGTCTGGTGAGGAAACTGCGCCTGCAGTTAGCAACTGATGTGAAGGAGGTCTTGGTTTTGGAGAAGGAGACTGCTAAGGTTGTGAAGGGGACCAAGAGAAAACTTTACGAAGTGGATGAGACTGTTAAAGCAGGATTCTTAGATATGCAGAAGAGGCAGGATGATATGCTCCAGCAAATGATGAAGAAACTAGAACGGAAGGATTTGGCAAACCAAGGTAACTTCTTGGAAAGATTTCTGAAAATTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

198

Amino Acids

22.67

Weight (kDa)

6.23

Isoelectric Point (pI)

30.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 14 - 139 4.6e-15 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 65, 170
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 1 cut(s) 588
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 2 cut(s) 57, 152
AfiI CCNNNNNNNGG 1 cut(s) 74
AflII CTTAAG 1 cut(s) 341
AgsI TTSAA 1 cut(s) 298
AjnI CCWGG 2 cut(s) 81, 123
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AluBI AGCT 2 cut(s) 256, 291
AluI AGCT 2 cut(s) 256, 291
Alw26I GTCTC 3 cut(s) 342, 407, 458
AoxI GGCC 1 cut(s) 202
ApeKI GCWGC 3 cut(s) 47, 50, 291
ApoI RAATTY 1 cut(s) 588
Asp700I GAANNNNTTC 1 cut(s) 579
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 62, 435
AsuHPI GGTGA 1 cut(s) 364
AvaII GGWCC 2 cut(s) 62, 435
BbvI GCAGC 3 cut(s) 59, 62, 278
BccI CCATC 2 cut(s) 169, 232
BceAI ACGGC 1 cut(s) 189
BciT130I CCWGG 2 cut(s) 83, 125
BcoDI GTCTC 3 cut(s) 342, 407, 458
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 2 cut(s) 45, 368
BfrI CTTAAG 1 cut(s) 341
BisI GCNGC 3 cut(s) 48, 51, 292
BlsI GCNGC 3 cut(s) 49, 52, 293
BmcAI AGTACT 2 cut(s) 57, 152
Bme1390I CCNGG 2 cut(s) 83, 125
Bme18I GGWCC 2 cut(s) 62, 435
BmgT120I GGNCC 2 cut(s) 62, 435
BmiI GGNNCC 1 cut(s) 436
BmrFI CCNGG 2 cut(s) 83, 125
BmsI GCATC 1 cut(s) 322
BpmI CTGGAG 1 cut(s) 500
Bpu10I CCTNAGC 2 cut(s) 287, 420
BpuEI CTTGAG 1 cut(s) 281
Bsa29I ATCGAT 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 2 cut(s) 141, 559
Bsc4I CCNNNNNNNGG 1 cut(s) 74
BseBI CCWGG 2 cut(s) 83, 125
BseCI ATCGAT 1 cut(s) 89
BseDI CCNNGG 2 cut(s) 141, 559
BseGI GGATG 4 cut(s) 111, 337, 466, 511
BseLI CCNNNNNNNGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 3 cut(s) 59, 62, 278
BshFI GGCC 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 89
BslFI GGGAC 2 cut(s) 102, 448
BslI CCNNNNNNNGG 1 cut(s) 74
BsmAI GTCTC 3 cut(s) 342, 407, 458
BsmBI CGTCTC 1 cut(s) 342
BsmFI GGGAC 2 cut(s) 102, 448
BsnI GGCC 1 cut(s) 204
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 2 cut(s) 65, 170
BspANI GGCC 1 cut(s) 204
BspDI ATCGAT 1 cut(s) 89
BspLI GGNNCC 1 cut(s) 436
BspMAI CTGCAG 2 cut(s) 49, 372
BspTI CTTAAG 1 cut(s) 341
BssECI CCNNGG 2 cut(s) 141, 559
BssMI GATC 1 cut(s) 181
BssNI GRCGYC 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 559
Bst2UI CCWGG 2 cut(s) 83, 125
Bst4CI ACNGT 2 cut(s) 145, 469
Bst6I CTCTTC 1 cut(s) 491
BstACI GRCGYC 1 cut(s) 78
BstAFI CTTAAG 1 cut(s) 341
BstAPI GCANNNNNTGC 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 202, 368
BstDEI CTNAG 4 cut(s) 154, 287, 420, 484
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 4 cut(s) 111, 337, 466, 511
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 1 cut(s) 184
BstMAI GTCTC 3 cut(s) 342, 407, 458
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 3 cut(s) 47, 332, 499
BstNI CCWGG 2 cut(s) 83, 125
BstSCI CCNGG 2 cut(s) 81, 123
BstSFI CTRYAG 2 cut(s) 45, 368
BstV1I GCAGC 3 cut(s) 59, 62, 278
Bsu15I ATCGAT 1 cut(s) 89
BsuRI GGCC 1 cut(s) 204
BsuTUI ATCGAT 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 4 cut(s) 111, 337, 466, 511
Cac8I GCNNGC 2 cut(s) 202, 368
CfoI GCGC 1 cut(s) 366
Cfr13I GGNCC 2 cut(s) 62, 435
ClaI ATCGAT 1 cut(s) 89
CseI GACGC 3 cut(s) 67, 121, 324
Csp6I GTAC 2 cut(s) 56, 151
CviAII CATG 1 cut(s) 190
CviJI RGCY 5 cut(s) 41, 200, 204, 256, 291
CviKI_1 RGCY 5 cut(s) 41, 200, 204, 256, 291
CviQI GTAC 2 cut(s) 56, 151
DdeI CTNAG 4 cut(s) 154, 287, 420, 484
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
Eco130I CCWWGG 1 cut(s) 559
Eco47I GGWCC 2 cut(s) 62, 435
EcoRII CCWGG 2 cut(s) 81, 123
EcoT14I CCWWGG 1 cut(s) 559
ErhI CCWWGG 1 cut(s) 559
Esp3I CGTCTC 1 cut(s) 342
FaeI CATG 1 cut(s) 193
FaiI YATR 4 cut(s) 186, 191, 491, 512
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 2 cut(s) 102, 448
FatI CATG 1 cut(s) 189
Fnu4HI GCNGC 3 cut(s) 48, 51, 292
FokI GGATG 4 cut(s) 118, 344, 473, 518
Fsp4HI GCNGC 3 cut(s) 48, 51, 292
FspBI CTAG 1 cut(s) 536
GlaI GCGC 1 cut(s) 365
GluI GCNGC 3 cut(s) 48, 51, 292
GsuI CTGGAG 1 cut(s) 500
HaeIII GGCC 1 cut(s) 204
HgaI GACGC 3 cut(s) 67, 121, 324
HhaI GCGC 1 cut(s) 366
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 1 cut(s) 193
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 5 cut(s) 86, 158, 166, 346, 480
HphI GGTGA 1 cut(s) 364
Hpy188I TCNGA 2 cut(s) 274, 585
Hpy188III TCNNGA 3 cut(s) 209, 260, 298
HpyAV CCTTC 5 cut(s) 68, 382, 403, 424, 538
HpyCH4III ACNGT 2 cut(s) 145, 469
HpyCH4V TGCA 4 cut(s) 47, 326, 370, 493
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 332, 499
HpyF3I CTNAG 4 cut(s) 154, 287, 420, 484
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 1 cut(s) 193
HspAI GCGC 1 cut(s) 364
Kzo9I GATC 1 cut(s) 181
LmnI GCTCC 2 cut(s) 241, 519
Lsp1109I GCAGC 3 cut(s) 59, 62, 278
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 536
MaeIII GTNAC 2 cut(s) 305, 563
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 3 cut(s) 191, 508, 541
MluCI AATT 1 cut(s) 588
MlyI GAGTC 2 cut(s) 167, 355
MmeI TCCRAC 2 cut(s) 83, 252
MnlI CCTC 6 cut(s) 31, 256, 268, 348, 385, 492
MroXI GAANNNNTTC 1 cut(s) 579
MseI TTAA 4 cut(s) 12, 279, 342, 471
MspCI CTTAAG 1 cut(s) 341
MspR9I CCNGG 2 cut(s) 83, 125
MvaI CCWGG 2 cut(s) 83, 125
MwoI GCNNNNNNNGC 3 cut(s) 47, 332, 499
NdeII GATC 1 cut(s) 181
NlaIII CATG 1 cut(s) 193
NlaIV GGNNCC 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 3 cut(s) 86, 166, 480
PfoI TCCNGGA 2 cut(s) 81, 123
PkrI GCNGC 3 cut(s) 49, 52, 293
PleI GAGTC 2 cut(s) 166, 354
PpsI GAGTC 2 cut(s) 166, 354
Psp6I CCWGG 2 cut(s) 81, 123
PspGI CCWGG 2 cut(s) 81, 123
PspN4I GGNNCC 1 cut(s) 436
PspPI GGNCC 2 cut(s) 62, 435
PstI CTGCAG 2 cut(s) 49, 372
RsaI GTAC 2 cut(s) 57, 152
RsaNI GTAC 2 cut(s) 56, 151
SaqAI TTAA 4 cut(s) 12, 279, 342, 471
SatI GCNGC 3 cut(s) 48, 51, 292
Sau3AI GATC 1 cut(s) 181
Sau96I GGNCC 2 cut(s) 62, 435
ScaI AGTACT 2 cut(s) 57, 152
SchI GAGTC 2 cut(s) 167, 355
ScrFI CCNGG 2 cut(s) 83, 125
SetI ASST 6 cut(s) 258, 279, 293, 396, 426, 565
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 2 cut(s) 45, 368
SinI GGWCC 2 cut(s) 62, 435
SmlI CTYRAG 2 cut(s) 260, 341
SmoI CTYRAG 2 cut(s) 260, 341
Sse9I AATT 1 cut(s) 588
SsiI CCGC 2 cut(s) 65, 170
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 2 cut(s) 81, 123
StyI CCWWGG 1 cut(s) 559
TaaI ACNGT 2 cut(s) 145, 469
TaqI TCGA 1 cut(s) 89
TasI AATT 1 cut(s) 588
TatI WGTACW 2 cut(s) 55, 150
TfiI GAWTC 3 cut(s) 86, 166, 480
Tru1I TTAA 4 cut(s) 12, 279, 342, 471
Tru9I TTAA 4 cut(s) 12, 279, 342, 471
TseI GCWGC 3 cut(s) 47, 50, 291
TspDTI ATGAA 3 cut(s) 206, 228, 542
TspGWI ACGGA 1 cut(s) 556
Vha464I CTTAAG 1 cut(s) 341
VpaK11BI GGWCC 2 cut(s) 62, 435
XapI RAATTY 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 1 cut(s) 536
ZrmI AGTACT 2 cut(s) 57, 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.