Rroxscaffold_2G00138860

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008832
Physical Location & Seq
Reverse (-)
77067263 .. 77068809
1547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138860.1

Sequence Viewer

Length: 1077 bp
ATGGTTAACGCCGGGGAGTTGTTTGCAGGGGCTGCTGTTGGAGGAGCATTTGGACCCGTGTTTGATGTCTTTAGGGAAGTGGTGGAGAAGACTTTCGAGTTTAATCCCACCCTCAAAAGCGTCAAATCCACAGTGGACTCTTTAGAGTTATTGATCCCGCAGATAGAAGGTCATAACAACGCGTTGGGTGGTAACCGAGAAGGGGAAGTAGAAAAGCTCAAAGAAACAATGGAGGAGGGGAAAAAGCTGATTGAAGAGCTGCTGGAGGTTAATGAGTGGAGCTGGTTGAAGGCTCGTTACACCGAACAACTTGTTGGGTTGGATGAGTCTCTCAAGAGACACTTGGGGATATTGCAGGTGCAGGGAGCGATTGATGGGAAGGAGGCCTTGGTTGTGGCAAAGGATACTAAAAAGGATGTGGAGGAGAGCTTGGCTTTGACAAAGGAACATACTCGGTTCCTGAAGGATATCTCGGTCACGGCGGAGGGAAACACCAACCAACTCGACAGATTCGAATCGGCTCTTTATGCACGACTGTCGGATCTACGGAAGGGGCAGGATGCAATGATAGCTACAATGACGTATAGAGTGAAACGTCATCGAATGGAAGACAAAGTGGGGTCGTTGATGGAAGCCGCTGCTCTAGGAGCTTCACTAGAACAATTGTATGATGTGGTTTCAACTCGTATGGACGAAGAGATCAAAAGGCGTCACTTAGGACGGATATTTGCAGGGACAAGTAAGATATTTGAATTGATCGAATGGAAGTGGGCAAAAAACAAAGAGATGTCGTATATTCCGACTGAGCAACTAAAGAATTTCAGGGTGGAGATAGAAGAGGGGGTAGAGCTTGTCCAGAACTGCACCAAGGTTCTTGAGTGGACCGACGACCTAAAGTCCGAGTACACCAACAAATTCCTTCATTTGGAGAAAACTCTCGAGAGACTCTACAACCGTTTAAATGGAATACACCCGTTTAAATGGAATGCTGGAAAATGCGGCAGCTCATGTGGAACGGCGTCCATGAGGGATTCTGATATCATTAACGATCGATTCAGTCATTCAGATCATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

358

Amino Acids

40.66

Weight (kDa)

5.56

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 141 5.8e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 1 cut(s) 346
AccII CGCG 1 cut(s) 182
AciI CCGC 4 cut(s) 158, 482, 636, 999
AclWI GGATC 2 cut(s) 148, 549
AcsI RAATTY 2 cut(s) 817, 914
AcuI CTGAAG 1 cut(s) 482
AcyI GRCGYC 2 cut(s) 709, 1019
AfaI GTAC 1 cut(s) 905
AfiI CCNNNNNNNGG 2 cut(s) 202, 925
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 4 cut(s) 254, 289, 681, 752
AhdI GACNNNNNGTC 1 cut(s) 895
AjuI GAANNNNNNNTTGG 4 cut(s) 297, 329, 371, 403
AluBI AGCT 9 cut(s) 217, 247, 259, 282, 429, 572, 650, 850, 1005
AluI AGCT 9 cut(s) 217, 247, 259, 282, 429, 572, 650, 850, 1005
Alw26I GTCTC 3 cut(s) 331, 333, 937
AlwI GGATC 2 cut(s) 148, 549
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 938
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 4 cut(s) 32, 259, 638, 1002
ApoI RAATTY 2 cut(s) 817, 914
Asp700I GAANNNNTTC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 53, 882
AsuC2I CCSGG 1 cut(s) 13
AsuII TTCGAA 1 cut(s) 513
AvaI CYCGRG 1 cut(s) 938
AvaII GGWCC 2 cut(s) 53, 882
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
BbsI GAAGAC 2 cut(s) 95, 615
BbvI GCAGC 4 cut(s) 19, 246, 625, 1014
BccI CCATC 2 cut(s) 368, 622
BceAI ACGGC 2 cut(s) 495, 1032
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BciVI GTATCC 1 cut(s) 397
BcnI CCSGG 1 cut(s) 13
BcoDI GTCTC 3 cut(s) 331, 333, 937
BfaI CTAG 2 cut(s) 644, 656
BfuAI ACCTGC 1 cut(s) 346
BfuI GTATCC 1 cut(s) 397
BisI GCNGC 6 cut(s) 33, 260, 636, 639, 1000, 1003
BlsI GCNGC 6 cut(s) 34, 261, 637, 640, 1001, 1004
Bme1390I CCNGG 1 cut(s) 13
Bme18I GGWCC 2 cut(s) 53, 882
BmeRI GACNNNNNGTC 1 cut(s) 895
BmeT110I CYCGRG 1 cut(s) 938
BmgT120I GGNCC 2 cut(s) 53, 882
BmiI GGNNCC 2 cut(s) 55, 458
BmrFI CCNGG 1 cut(s) 13
BmsI GCATC 1 cut(s) 550
BpiI GAAGAC 2 cut(s) 95, 615
BpmI CTGGAG 1 cut(s) 284
Bpu14I TTCGAA 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 317, 896
BpuMI CCSGG 1 cut(s) 13
Bsa29I ATCGAT 1 cut(s) 1051
BsaBI GATNNNNATC 1 cut(s) 514
BsaHI GRCGYC 2 cut(s) 709, 1019
BsaJI CCNNGG 3 cut(s) 12, 387, 867
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 925
Bse3DI GCAATG 1 cut(s) 570
Bse8I GATNNNNATC 1 cut(s) 514
BseCI ATCGAT 1 cut(s) 1051
BseDI CCNNGG 3 cut(s) 12, 387, 867
BseGI GGATG 3 cut(s) 328, 421, 565
BseJI GATNNNNATC 1 cut(s) 514
BseLI CCNNNNNNNGG 2 cut(s) 202, 925
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 1 cut(s) 795
BseRI GAGGAG 3 cut(s) 57, 248, 437
BseXI GCAGC 4 cut(s) 19, 246, 625, 1014
BsgI GTGCAG 2 cut(s) 380, 847
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 1 cut(s) 1051
BshFI GGCC 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 1051
BsiEI CGRYCG 1 cut(s) 1051
BsiHKCI CYCGRG 1 cut(s) 938
BsiSI CCGG 1 cut(s) 12
BslFI GGGAC 1 cut(s) 748
BslI CCNNNNNNNGG 2 cut(s) 202, 925
BsmAI GTCTC 3 cut(s) 331, 333, 937
BsmFI GGGAC 1 cut(s) 748
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 1 cut(s) 386
BsoBI CYCGRG 1 cut(s) 938
Bsp119I TTCGAA 1 cut(s) 513
Bsp143I GATC 6 cut(s) 153, 541, 699, 756, 1048, 1066
BspACI CCGC 4 cut(s) 158, 482, 636, 999
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 1 cut(s) 796
BspDI ATCGAT 1 cut(s) 1051
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 2 cut(s) 55, 458
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 2 cut(s) 148, 549
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 570
BssECI CCNNGG 3 cut(s) 12, 387, 867
BssMI GATC 6 cut(s) 153, 541, 699, 756, 1048, 1066
BssNI GRCGYC 2 cut(s) 709, 1019
BssT1I CCWWGG 2 cut(s) 387, 867
Bst4CI ACNGT 3 cut(s) 133, 537, 956
Bst6I CTCTTC 3 cut(s) 249, 690, 831
BstACI GRCGYC 2 cut(s) 709, 1019
BstAPI GCANNNNNTGC 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 513
BstDEI CTNAG 2 cut(s) 715, 804
BstEII GGTNACC 1 cut(s) 191
BstF5I GGATG 3 cut(s) 328, 421, 565
BstFNI CGCG 1 cut(s) 182
BstKTI GATC 6 cut(s) 156, 544, 702, 759, 1051, 1069
BstMAI GTCTC 3 cut(s) 331, 333, 937
BstMBI GATC 6 cut(s) 153, 541, 699, 756, 1048, 1066
BstMCI CGRYCG 1 cut(s) 1051
BstMWI GCNNNNNNNGC 4 cut(s) 32, 527, 569, 647
BstPI GGTNACC 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 11
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 4 cut(s) 19, 246, 625, 1014
BstV2I GAAGAC 2 cut(s) 95, 615
BstX2I RGATCY 1 cut(s) 541
BstYI RGATCY 1 cut(s) 541
Bsu15I ATCGAT 1 cut(s) 1051
BsuI GTATCC 1 cut(s) 397
BsuRI GGCC 1 cut(s) 386
BsuTUI ATCGAT 1 cut(s) 1051
BtsCI GGATG 3 cut(s) 328, 421, 565
BtsIMutI CAGTG 1 cut(s) 138
BveI ACCTGC 1 cut(s) 346
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 53, 882
ClaI ATCGAT 1 cut(s) 1051
CseI GACGC 3 cut(s) 109, 698, 1008
Csp6I GTAC 1 cut(s) 904
CviAII CATG 3 cut(s) 1008, 1024, 1070
CviQI GTAC 1 cut(s) 904
DdeI CTNAG 2 cut(s) 715, 804
DpnI GATC 6 cut(s) 155, 543, 701, 758, 1050, 1068
DpnII GATC 6 cut(s) 153, 541, 699, 756, 1048, 1066
DraI TTTAAA 2 cut(s) 960, 979
DriI GACNNNNNGTC 1 cut(s) 895
Eam1104I CTCTTC 3 cut(s) 249, 690, 831
Eam1105I GACNNNNNGTC 1 cut(s) 895
EarI CTCTTC 3 cut(s) 249, 690, 831
EciI GGCGGA 1 cut(s) 497
Eco130I CCWWGG 2 cut(s) 387, 867
Eco147I AGGCCT 1 cut(s) 386
Eco32I GATATC 2 cut(s) 469, 1039
Eco47I GGWCC 2 cut(s) 53, 882
Eco57I CTGAAG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 938
Eco91I GGTNACC 1 cut(s) 191
EcoO65I GGTNACC 1 cut(s) 191
EcoRV GATATC 2 cut(s) 469, 1039
EcoT14I CCWWGG 2 cut(s) 387, 867
ErhI CCWWGG 2 cut(s) 387, 867
FaeI CATG 3 cut(s) 1011, 1027, 1073
FalI AAGNNNNNCTT 4 cut(s) 326, 358, 371, 403
FaqI GGGAC 1 cut(s) 748
FatI CATG 3 cut(s) 1007, 1023, 1069
FauI CCCGC 1 cut(s) 165
Fnu4HI GCNGC 6 cut(s) 33, 260, 636, 639, 1000, 1003
FokI GGATG 3 cut(s) 335, 428, 572
Fsp4HI GCNGC 6 cut(s) 33, 260, 636, 639, 1000, 1003
FspBI CTAG 2 cut(s) 644, 656
GluI GCNGC 6 cut(s) 33, 260, 636, 639, 1000, 1003
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 1 cut(s) 386
HapII CCGG 1 cut(s) 12
HgaI GACGC 3 cut(s) 109, 698, 1008
Hin1I GRCGYC 2 cut(s) 709, 1019
Hin1II CATG 3 cut(s) 1011, 1027, 1073
HincII GTYRAC 1 cut(s) 7
HindII GTYRAC 1 cut(s) 7
HinfI GANTC 7 cut(s) 137, 326, 510, 515, 945, 1031, 1053
HpaI GTTAAC 1 cut(s) 7
HpaII CCGG 1 cut(s) 12
Hpy166II GTNNAC 4 cut(s) 7, 136, 882, 906
Hpy188I TCNGA 5 cut(s) 541, 801, 901, 1036, 1066
Hpy188III TCNNGA 6 cut(s) 334, 460, 856, 875, 938, 940
Hpy8I GTNNAC 4 cut(s) 7, 136, 882, 906
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 7 cut(s) 161, 194, 283, 373, 457, 544, 929
HpyCH4III ACNGT 3 cut(s) 133, 537, 956
HpyCH4IV ACGT 2 cut(s) 581, 595
HpyCH4V TGCA 7 cut(s) 26, 355, 361, 530, 563, 731, 864
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 527, 569, 647
HpyF3I CTNAG 2 cut(s) 715, 804
HpySE526I ACGT 2 cut(s) 581, 595
Hsp92I GRCGYC 2 cut(s) 709, 1019
Hsp92II CATG 3 cut(s) 1011, 1027, 1073
KspAI GTTAAC 1 cut(s) 7
Kzo9I GATC 6 cut(s) 153, 541, 699, 756, 1048, 1066
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 4 cut(s) 44, 279, 365, 647
Lsp1109I GCAGC 4 cut(s) 19, 246, 625, 1014
LweI GCATC 1 cut(s) 550
MaeI CTAG 2 cut(s) 644, 656
MaeII ACGT 2 cut(s) 581, 595
MaeIII GTNAC 4 cut(s) 191, 296, 475, 710
MalI GATC 6 cut(s) 155, 543, 701, 758, 1050, 1068
MboI GATC 6 cut(s) 153, 541, 699, 756, 1048, 1066
MboII GAAGA 5 cut(s) 100, 266, 620, 707, 848
MfeI CAATTG 1 cut(s) 662
MflI RGATCY 1 cut(s) 541
MluCI AATT 4 cut(s) 662, 752, 817, 914
MluI ACGCGT 1 cut(s) 180
MlyI GAGTC 3 cut(s) 131, 335, 939
MmeI TCCRAC 4 cut(s) 19, 300, 519, 824
MroXI GAANNNNTTC 1 cut(s) 92
MseI TTAA 6 cut(s) 6, 102, 270, 959, 978, 1044
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 1 cut(s) 13
MunI CAATTG 1 cut(s) 662
Mva1269I GAATGC 1 cut(s) 991
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 32, 527, 569, 647
NciI CCSGG 1 cut(s) 13
NdeII GATC 6 cut(s) 153, 541, 699, 756, 1048, 1066
NlaIII CATG 3 cut(s) 1011, 1027, 1073
NlaIV GGNNCC 2 cut(s) 55, 458
NmuCI GTSAC 2 cut(s) 475, 710
NspV TTCGAA 1 cut(s) 513
PaeR7I CTCGAG 1 cut(s) 938
PaqCI CACCTGC 1 cut(s) 346
PceI AGGCCT 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 510, 797
PctI GAATGC 1 cut(s) 991
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 4 cut(s) 510, 515, 1031, 1053
PkrI GCNGC 6 cut(s) 34, 261, 637, 640, 1001, 1004
Ple19I CGATCG 1 cut(s) 1051
PleI GAGTC 3 cut(s) 131, 334, 939
PpsI GAGTC 3 cut(s) 131, 334, 939
PspEI GGTNACC 1 cut(s) 191
PspN4I GGNNCC 2 cut(s) 55, 458
PspPI GGNCC 2 cut(s) 53, 882
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 1 cut(s) 541
PvuI CGATCG 1 cut(s) 1051
RsaI GTAC 1 cut(s) 905
RsaNI GTAC 1 cut(s) 904
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 6 cut(s) 6, 102, 270, 959, 978, 1044
SatI GCNGC 6 cut(s) 33, 260, 636, 639, 1000, 1003
Sau3AI GATC 6 cut(s) 153, 541, 699, 756, 1048, 1066
Sau96I GGNCC 2 cut(s) 53, 882
SchI GAGTC 3 cut(s) 131, 335, 939
ScrFI CCNGG 1 cut(s) 13
SfaNI GCATC 1 cut(s) 550
Sfr274I CTCGAG 1 cut(s) 938
SfuI TTCGAA 1 cut(s) 513
SinI GGWCC 2 cut(s) 53, 882
SlaI CTCGAG 1 cut(s) 938
SmlI CTYRAG 3 cut(s) 332, 875, 938
SmoI CTYRAG 3 cut(s) 332, 875, 938
Sse9I AATT 4 cut(s) 662, 752, 817, 914
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 4 cut(s) 158, 482, 636, 999
SspMI CTAG 2 cut(s) 644, 656
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 1 cut(s) 11
StyI CCWWGG 2 cut(s) 387, 867
TaaI ACNGT 3 cut(s) 133, 537, 956
TaiI ACGT 2 cut(s) 584, 598
TaqI TCGA 7 cut(s) 96, 504, 513, 601, 759, 939, 1051
TaqII GACCGA 2 cut(s) 463, 899
TasI AATT 4 cut(s) 662, 752, 817, 914
TatI WGTACW 1 cut(s) 903
TauI GCSGC 2 cut(s) 638, 1002
TfiI GAWTC 4 cut(s) 510, 515, 1031, 1053
Tru1I TTAA 6 cut(s) 6, 102, 270, 959, 978, 1044
Tru9I TTAA 6 cut(s) 6, 102, 270, 959, 978, 1044
TscAI CASTG 1 cut(s) 138
TseFI GTSAC 2 cut(s) 475, 710
TseI GCWGC 4 cut(s) 32, 259, 638, 1002
Tsp45I GTSAC 2 cut(s) 475, 710
TspDTI ATGAA 1 cut(s) 911
TspGWI ACGGA 2 cut(s) 562, 736
TspRI CASTG 1 cut(s) 138
VpaK11BI GGWCC 2 cut(s) 53, 882
XapI RAATTY 2 cut(s) 817, 914
XhoI CTCGAG 1 cut(s) 938
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 2 cut(s) 644, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.