FvH4_1g25060

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001836
Physical Location & Seq
Reverse (-)
16852714 .. 16857060
4347 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25060.t1

Sequence Viewer

Length: 1365 bp
ATGTTCAAGTCGAAGCTGCAGGAGGTGTGCCAGAAGAAGGCTTGGGCCTTACCGGAGTACAGAAGCACCAAGGAGGGCGTCGATCACGACCCTCGCTTCGCCGCCTCCGTCCTCGTCAACGGCCTCACTTTCCACACTCCCCAATCCTTCCGCTCCTCCAAGCTCGCCCAAAACGACGCCGCTCGCCTCGCATTCGAGCACTTTTCTGCCGCGCCACTCCCTGTTTCCCTCCCCGCTCCTTCCTCTTTCCCTCAGCCCAGTCTTATCGCAGGACTTCCCGTAGGATCTCAGATCAGCGACAATATGACAGTTGTCGGTTCTCAGAACAGTGGCAGCTCATTAAGTGTTGACGGTGATAACTATTCTAAAGATGTACAGCATCATAAGAACCAGCTGCAATATTATGCTCAAAAGAGGAACCTAAATTTACCTGTGTACTCTTGTGAGCGTGAAGGTCCTCCTCATGCCACTCGTTTCAAATGTACAGTAACAATCGATGGACAGACTTATCAGGGTCAAGAATTTCTTCCCACTGTGAAGGATGCTGAACATGCAGCTGCAAAAGTTGCTTTGATCTCCCTGTTACCAAATGGAGTCCAACAAGATGATACTGGTTTGTACAAGAACCTTCTACAAGAACTACTTCAAAAAGAAGGTCAACATGTACCACTTTATAGCTCAACGACTTCGGGTGAACCTCACATGCTTAGGTTTGTTTCAACCGTGGAGATTGGAGGAGAATACTTTACAGGTGAGGTAGCAAGAAGCAAGAAGCTGGCAGAAGTGAGTGCAGCAAAGGTTGCTTACAACACTCTGAGAGATCGTAAGTTGAGTCATATTCCATTGTTTCCCACCCCGGCTATTGAAGGGCAAGTCTGTTCTGAGTTATTATCATCTAAGGCCCCATCAGTTGTAGCGGCTGATCTACAACAACAAATGAGACCCAACACTCCAATTCCAAATTCGGTTAAAAGGGAACCAGAATCTGATCTACAACAACAAATGAGACCCGACACTCCAATTCCAAATTCGGTTAAAAGGGAACCAGAATCAGAGATGAGTGTGGCCACAGTGGGAACCAGTTGTTTGGGGACAACTCCATCTTTGGGAGTTATTAGTGTTACTGTTGGTGGAGGATCCACATTATCTGGCACTGTACATGCCCGTCAACCGGAGACTTCACGTTCTGCTTCCCCTGCGCCCCAAAACGGTTCATTTTTGACTTTGGCTTCTGATTGTAACTCAAATGCGGACTCTACTCTTGAGCCACCAGCGAAGAGATTCTTCTCTAACAAGGTTTCTGTGCTTCCACGGATGGCGGCGACACTCCCGGAAGACTGCGTCGTGTTGCCCCTTAGTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0098586 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

455

Amino Acids

48.98

Weight (kDa)

6.84

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 3 - 66 3.8e-10 Double-stranded RNA binding motif
dsrm PF00035 129 - 193 1.3e-14 Double-stranded RNA binding motif
dsrm PF00035 207 - 272 5.6e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 153, 182, 236
AccII CGCG 1 cut(s) 212
AciI CCGC 8 cut(s) 102, 151, 180, 210, 234, 917, 1250, 1319
AclWI GGATC 3 cut(s) 292, 1131, 1144
AcoI YGGCCR 1 cut(s) 1065
AcsI RAATTY 4 cut(s) 424, 521, 961, 1027
AcyI GRCGYC 2 cut(s) 78, 177
AfaI GTAC 7 cut(s) 59, 375, 437, 484, 620, 666, 1158
AfiI CCNNNNNNNGG 4 cut(s) 37, 1106, 1171, 1208
AflIII ACRYGT 1 cut(s) 661
AgsI TTSAA 5 cut(s) 7, 478, 647, 720, 866
AjuI GAANNNNNNNTTGG 4 cut(s) 946, 978, 1012, 1044
AluBI AGCT 7 cut(s) 16, 163, 336, 394, 557, 678, 775
AluI AGCT 7 cut(s) 16, 163, 336, 394, 557, 678, 775
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 3 cut(s) 934, 1000, 1169
AlwI GGATC 3 cut(s) 292, 1131, 1144
AlwNI CAGNNNCTG 1 cut(s) 986
AoxI GGCC 4 cut(s) 45, 121, 900, 1065
ApeKI GCWGC 6 cut(s) 16, 333, 394, 554, 557, 791
ApoI RAATTY 4 cut(s) 424, 521, 961, 1027
ArsI GACNNNNNNTTYG 4 cut(s) 80, 112, 640, 672
Asp700I GAANNNNTTC 3 cut(s) 525, 642, 1280
AspLEI GCGC 2 cut(s) 214, 1201
AspS9I GGNCC 3 cut(s) 45, 455, 901
AsuC2I CCSGG 2 cut(s) 857, 1331
AsuHPI GGTGA 3 cut(s) 365, 704, 764
AvaII GGWCC 1 cut(s) 455
BalI TGGCCA 1 cut(s) 1067
BamHI GGATCC 1 cut(s) 1136
BbsI GAAGAC 1 cut(s) 1341
Bbv12I GWGCWC 1 cut(s) 201
BbvCI CCTCAGC 1 cut(s) 252
BbvI GCAGC 6 cut(s) 3, 345, 381, 544, 566, 803
BccI CCATC 4 cut(s) 491, 913, 1108, 1309
BceAI ACGGC 1 cut(s) 136
BcnI CCSGG 2 cut(s) 857, 1331
BcoDI GTCTC 3 cut(s) 934, 1000, 1169
BfmI CTRYAG 1 cut(s) 17
Bme1390I CCNGG 2 cut(s) 857, 1331
Bme18I GGWCC 1 cut(s) 455
BmgT120I GGNCC 3 cut(s) 45, 455, 901
BmiI GGNNCC 6 cut(s) 419, 903, 978, 1044, 1078, 1138
BmrFI CCNGG 2 cut(s) 857, 1331
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 2 cut(s) 388, 532
BmuI ACTGGG 1 cut(s) 252
BoxI GACNNNNGTC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 1341
Bpu10I CCTNAGC 2 cut(s) 252, 707
BpuEI CTTGAG 1 cut(s) 1283
BpuMI CCSGG 2 cut(s) 857, 1331
Bsa29I ATCGAT 1 cut(s) 495
BsaHI GRCGYC 2 cut(s) 78, 177
BsaI GGTCTC 2 cut(s) 934, 1000
BsaJI CCNNGG 4 cut(s) 69, 723, 855, 1310
BsaWI WCCGGW 2 cut(s) 52, 1171
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 1106, 1171, 1208
Bse1I ACTGG 3 cut(s) 258, 616, 1080
BseCI ATCGAT 1 cut(s) 495
BseDI CCNNGG 4 cut(s) 69, 723, 855, 1310
BseGI GGATG 2 cut(s) 547, 1320
BseLI CCNNNNNNNGG 4 cut(s) 37, 1106, 1171, 1208
BseMII CTCAG 5 cut(s) 266, 302, 335, 806, 873
BseNI ACTGG 3 cut(s) 258, 616, 1080
BseRI GAGGAG 3 cut(s) 145, 450, 750
BseXI GCAGC 6 cut(s) 3, 345, 381, 544, 566, 803
BsgI GTGCAG 1 cut(s) 810
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 4 cut(s) 47, 123, 902, 1067
BshVI ATCGAT 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 4 cut(s) 53, 857, 1172, 1331
BslFI GGGAC 1 cut(s) 1105
BslI CCNNNNNNNGG 4 cut(s) 37, 1106, 1171, 1208
BsmAI GTCTC 3 cut(s) 934, 1000, 1169
BsmFI GGGAC 1 cut(s) 1105
BsmI GAATGC 1 cut(s) 191
BsnI GGCC 4 cut(s) 47, 123, 902, 1067
Bso31I GGTCTC 2 cut(s) 934, 1000
Bsp1286I GDGCHC 1 cut(s) 201
Bsp1407I TGTACA 4 cut(s) 373, 482, 618, 1156
Bsp143I GATC 8 cut(s) 82, 284, 291, 573, 820, 922, 988, 1136
BspACI CCGC 8 cut(s) 102, 151, 180, 210, 234, 917, 1250, 1319
BspANI GGCC 4 cut(s) 47, 123, 902, 1067
BspCNI CTCAG 5 cut(s) 265, 301, 334, 807, 874
BspDI ATCGAT 1 cut(s) 495
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 6 cut(s) 419, 903, 978, 1044, 1078, 1138
BspMAI CTGCAG 1 cut(s) 21
BspPI GGATC 3 cut(s) 292, 1131, 1144
BspTNI GGTCTC 2 cut(s) 934, 1000
BsrBI CCGCTC 3 cut(s) 153, 182, 236
BsrGI TGTACA 4 cut(s) 373, 482, 618, 1156
BsrI ACTGG 3 cut(s) 258, 616, 1080
BssECI CCNNGG 4 cut(s) 69, 723, 855, 1310
BssMI GATC 8 cut(s) 82, 284, 291, 573, 820, 922, 988, 1136
BssNI GRCGYC 2 cut(s) 78, 177
BssT1I CCWWGG 1 cut(s) 69
Bst6I CTCTTC 1 cut(s) 1271
BstACI GRCGYC 2 cut(s) 78, 177
BstAPI GCANNNNNTGC 2 cut(s) 566, 800
BstAUI TGTACA 4 cut(s) 373, 482, 618, 1156
BstC8I GCNNGC 3 cut(s) 165, 184, 777
BstDEI CTNAG 9 cut(s) 252, 288, 321, 707, 815, 882, 897, 1355, 1362
BstDSI CCRYGG 2 cut(s) 723, 1310
BstF5I GGATG 2 cut(s) 547, 1320
BstFNI CGCG 1 cut(s) 212
BstHHI GCGC 2 cut(s) 214, 1201
BstKTI GATC 8 cut(s) 85, 287, 294, 576, 823, 925, 991, 1139
BstMAI GTCTC 3 cut(s) 934, 1000, 1169
BstMBI GATC 8 cut(s) 82, 284, 291, 573, 820, 922, 988, 1136
BstMWI GCNNNNNNNGC 5 cut(s) 188, 551, 566, 800, 1196
BstNSI RCATGY 4 cut(s) 554, 665, 706, 1163
BstPAI GACNNNNGTC 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 855, 1329
BstSFI CTRYAG 1 cut(s) 17
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 6 cut(s) 3, 345, 381, 544, 566, 803
BstV2I GAAGAC 1 cut(s) 1341
BstX2I RGATCY 2 cut(s) 284, 1136
BstXI CCANNNNNNTGG 1 cut(s) 1087
BstYI RGATCY 2 cut(s) 284, 1136
Bsu15I ATCGAT 1 cut(s) 495
BsuRI GGCC 4 cut(s) 47, 123, 902, 1067
BsuTUI ATCGAT 1 cut(s) 495
BtgI CCRYGG 2 cut(s) 723, 1310
BtsCI GGATG 2 cut(s) 547, 1320
BtsIMutI CAGTG 4 cut(s) 334, 531, 1077, 1152
Cac8I GCNNGC 3 cut(s) 165, 184, 777
CaiI CAGNNNCTG 1 cut(s) 986
CfoI GCGC 2 cut(s) 214, 1201
Cfr13I GGNCC 3 cut(s) 45, 455, 901
ClaI ATCGAT 1 cut(s) 495
CseI GACGC 3 cut(s) 67, 185, 1330
Csp6I GTAC 7 cut(s) 58, 374, 436, 483, 619, 665, 1157
CviAII CATG 5 cut(s) 464, 551, 662, 703, 1160
CviQI GTAC 7 cut(s) 58, 374, 436, 483, 619, 665, 1157
DdeI CTNAG 9 cut(s) 252, 288, 321, 707, 815, 882, 897, 1355, 1362
DpnI GATC 8 cut(s) 84, 286, 293, 575, 822, 924, 990, 1138
DpnII GATC 8 cut(s) 82, 284, 291, 573, 820, 922, 988, 1136
EaeI YGGCCR 1 cut(s) 1065
Eam1104I CTCTTC 1 cut(s) 1271
EarI CTCTTC 1 cut(s) 1271
Eco130I CCWWGG 1 cut(s) 69
Eco31I GGTCTC 2 cut(s) 934, 1000
Eco47I GGWCC 1 cut(s) 455
EcoO109I RGGNCCY 2 cut(s) 455, 901
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 5 cut(s) 467, 554, 665, 706, 1163
FalI AAGNNNNNCTT 6 cut(s) 510, 542, 627, 659, 1268, 1300
FaqI GGGAC 1 cut(s) 1105
FatI CATG 5 cut(s) 463, 550, 661, 702, 1159
FauI CCCGC 1 cut(s) 241
FokI GGATG 2 cut(s) 554, 1327
GlaI GCGC 2 cut(s) 213, 1200
HaeIII GGCC 4 cut(s) 47, 123, 902, 1067
HapII CCGG 4 cut(s) 53, 857, 1172, 1331
HgaI GACGC 3 cut(s) 67, 185, 1330
HhaI GCGC 2 cut(s) 214, 1201
Hin1I GRCGYC 2 cut(s) 78, 177
Hin1II CATG 5 cut(s) 467, 554, 665, 706, 1163
Hin6I GCGC 2 cut(s) 212, 1199
HinP1I GCGC 2 cut(s) 212, 1199
HincII GTYRAC 4 cut(s) 118, 349, 659, 1169
HindII GTYRAC 4 cut(s) 118, 349, 659, 1169
HinfI GANTC 6 cut(s) 594, 832, 983, 1049, 1253, 1281
HpaII CCGG 4 cut(s) 53, 857, 1172, 1331
HphI GGTGA 3 cut(s) 365, 704, 764
Hpy166II GTNNAC 6 cut(s) 118, 349, 436, 659, 695, 1169
Hpy188I TCNGA 7 cut(s) 291, 324, 816, 883, 988, 1054, 1234
Hpy188III TCNNGA 3 cut(s) 86, 518, 1262
Hpy8I GTNNAC 6 cut(s) 118, 349, 436, 659, 695, 1169
Hpy99I CGWCG 3 cut(s) 83, 179, 1346
HpyAV CCTTC 8 cut(s) 31, 157, 249, 446, 532, 638, 647, 860
HpyCH4IV ACGT 1 cut(s) 1183
HpyCH4V TGCA 5 cut(s) 19, 397, 554, 560, 791
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 551, 566, 800, 1196
HpyF3I CTNAG 9 cut(s) 252, 288, 321, 707, 815, 882, 897, 1355, 1362
HpySE526I ACGT 1 cut(s) 1183
Hsp92I GRCGYC 2 cut(s) 78, 177
Hsp92II CATG 5 cut(s) 467, 554, 665, 706, 1163
HspAI GCGC 2 cut(s) 212, 1199
Kzo9I GATC 8 cut(s) 82, 284, 291, 573, 820, 922, 988, 1136
LmnI GCTCC 2 cut(s) 158, 241
Lsp1109I GCAGC 6 cut(s) 3, 345, 381, 544, 566, 803
LweI GCATC 2 cut(s) 388, 532
MaeII ACGT 1 cut(s) 1183
MaeIII GTNAC 4 cut(s) 487, 582, 1120, 1238
MalI GATC 8 cut(s) 84, 286, 293, 575, 822, 924, 990, 1138
MbiI CCGCTC 3 cut(s) 153, 182, 236
MboI GATC 8 cut(s) 82, 284, 291, 573, 820, 922, 988, 1136
MboII GAAGA 5 cut(s) 46, 518, 1276, 1288, 1346
MflI RGATCY 2 cut(s) 284, 1136
MhlI GDGCHC 1 cut(s) 201
MlsI TGGCCA 1 cut(s) 1067
MluCI AATT 6 cut(s) 424, 521, 954, 961, 1020, 1027
MluNI TGGCCA 1 cut(s) 1067
MlyI GAGTC 3 cut(s) 603, 841, 1247
MmeI TCCRAC 1 cut(s) 622
Mox20I TGGCCA 1 cut(s) 1067
MroXI GAANNNNTTC 3 cut(s) 525, 642, 1280
MscI TGGCCA 1 cut(s) 1067
MseI TTAA 3 cut(s) 341, 969, 1035
Msp20I TGGCCA 1 cut(s) 1067
MspA1I CMGCKG 2 cut(s) 394, 557
MspI CCGG 4 cut(s) 53, 857, 1172, 1331
MspR9I CCNGG 2 cut(s) 857, 1331
Mva1269I GAATGC 1 cut(s) 191
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 5 cut(s) 188, 551, 566, 800, 1196
NciI CCSGG 2 cut(s) 857, 1331
NdeII GATC 8 cut(s) 82, 284, 291, 573, 820, 922, 988, 1136
NlaIII CATG 5 cut(s) 467, 554, 665, 706, 1163
NlaIV GGNNCC 6 cut(s) 419, 903, 978, 1044, 1078, 1138
NspI RCATGY 4 cut(s) 554, 665, 706, 1163
PciI ACATGT 1 cut(s) 661
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 171
PctI GAATGC 1 cut(s) 191
PdmI GAANNNNTTC 3 cut(s) 525, 642, 1280
PfeI GAWTC 3 cut(s) 983, 1049, 1281
PflFI GACNNNGTC 1 cut(s) 1340
PfoI TCCNGGA 1 cut(s) 1329
PleI GAGTC 3 cut(s) 602, 840, 1247
PpsI GAGTC 3 cut(s) 602, 840, 1247
PpuMI RGGWCCY 1 cut(s) 455
PscI ACATGT 1 cut(s) 661
PshAI GACNNNNGTC 1 cut(s) 311
Psp5II RGGWCCY 1 cut(s) 455
PspN4I GGNNCC 6 cut(s) 419, 903, 978, 1044, 1078, 1138
PspPI GGNCC 3 cut(s) 45, 455, 901
PspPPI RGGWCCY 1 cut(s) 455
PstI CTGCAG 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 986
PsuI RGATCY 2 cut(s) 284, 1136
PsyI GACNNNGTC 1 cut(s) 1340
PvuII CAGCTG 2 cut(s) 394, 557
RsaI GTAC 7 cut(s) 59, 375, 437, 484, 620, 666, 1158
RsaNI GTAC 7 cut(s) 58, 374, 436, 483, 619, 665, 1157
SaqAI TTAA 3 cut(s) 341, 969, 1035
Sau3AI GATC 8 cut(s) 82, 284, 291, 573, 820, 922, 988, 1136
Sau96I GGNCC 3 cut(s) 45, 455, 901
SchI GAGTC 3 cut(s) 603, 841, 1247
ScrFI CCNGG 2 cut(s) 857, 1331
SduI GDGCHC 1 cut(s) 201
SfaNI GCATC 2 cut(s) 388, 532
SfcI CTRYAG 1 cut(s) 17
SinI GGWCC 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 1262
SmoI CTYRAG 1 cut(s) 1262
Sse9I AATT 6 cut(s) 424, 521, 954, 961, 1020, 1027
SsiI CCGC 8 cut(s) 102, 151, 180, 210, 234, 917, 1250, 1319
SspI AATATT 1 cut(s) 401
StyD4I CCNGG 2 cut(s) 855, 1329
StyI CCWWGG 1 cut(s) 69
TaiI ACGT 1 cut(s) 1186
TaqI TCGA 4 cut(s) 11, 81, 195, 495
TasI AATT 6 cut(s) 424, 521, 954, 961, 1020, 1027
TatI WGTACW 6 cut(s) 57, 373, 435, 482, 618, 1156
TauI GCSGC 5 cut(s) 104, 182, 212, 920, 1322
TfiI GAWTC 3 cut(s) 983, 1049, 1281
Tru1I TTAA 3 cut(s) 341, 969, 1035
Tru9I TTAA 3 cut(s) 341, 969, 1035
TscAI CASTG 4 cut(s) 334, 538, 1077, 1159
TseI GCWGC 6 cut(s) 16, 333, 394, 554, 557, 791
TspDTI ATGAA 1 cut(s) 1203
TspGWI ACGGA 2 cut(s) 97, 1327
TspRI CASTG 4 cut(s) 334, 538, 1077, 1159
Tth111I GACNNNGTC 1 cut(s) 1340
VpaK11BI GGWCC 1 cut(s) 455
XapI RAATTY 4 cut(s) 424, 521, 961, 1027
XceI RCATGY 4 cut(s) 554, 665, 706, 1163
XmnI GAANNNNTTC 3 cut(s) 525, 642, 1280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.