Rorug02G0273300

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001836
Physical Location & Seq
Forward (+)
29220448 .. 29225449
5002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0273300.1

Sequence Viewer

Length: 780 bp
ATGGGCACAAGTGTGGAGGATGTGGTGATAGTCGGAGGAGGGATAGCCGGGCTGGCGACAGCGGTGGCGCTGAAGAGAGCTGGAATAGAAGCGTTGGTGTTGGAGAGATCGGAAGGGCTGCGAGCCACTGGTGCAGCCTTGGGTCTATTCCCAAACGCCTGGTGTGCTCTCGATGCCTTGGGGGTTTCTCACAAGCTTGCTTCTTATACCCCCTCTCTCAAGAATTATGTAACTGATCTTGATACTGGAGAAGTTCAAGAAGTCTCTTTCACCGAAGCCAATGGGGATGCAGTTGGACCGAGAACAGTACATCGAAAAGCCTTGCTCGAGGCTTTGGCAGATGAATTATCGGTTCATTCAATCCGTTTCTCTTCCAAGATCTCTGCTATCGAAACCCAACAACATGAAGGTTCATCCATTGCCATTCTTCACATGGAAAATGGAACTGTCATTAAAGCAAAGGTTCTCATAGGGTGTGACGGGGTTCACTCAGTGGTGTCACGCTGGTTAGGACTCCGTGAACCAGTCTTTTCAGGTCGATCAGCTGTTCGTGGCTTGGCTATTTATCCTCAAGGCCATGGACTCAAGCAGAACGTGCGACAGTATGTAGGATCAGGCAGAAGGGCTGGCTTCGTTCCCCTCACCGACAAAGAGATCTATTGGTTCTTCTCTAGCATATCTCCAGCAAAAGGGACCTCCCTGGCAGAAGACCCTGAAGATATACAAAAAGAAGTACTTGAGAATTATGCCAAGGACCTCCCACCTATAGGGAAATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0098586 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

259

Amino Acids

27.61

Weight (kDa)

6.18

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 7 - 252 7.9e-20 FAD binding domain
DAO PF01266 7 - 55 4.8e-09 FAD dependent oxidoreductase
Lycopene_cycl PF05834 7 - 164 4.3e-06 Lycopene cyclase protein
FAD_oxidored PF12831 7 - 159 3.7e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 10 - 40 2.4e-06 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 62
AclWI GGATC 1 cut(s) 619
AcsI RAATTY 1 cut(s) 773
AcuI CTGAAG 2 cut(s) 92, 735
AdeI CACNNNGTG 1 cut(s) 493
AfaI GTAC 2 cut(s) 309, 735
AfiI CCNNNNNNNGG 2 cut(s) 689, 767
AgsI TTSAA 2 cut(s) 257, 360
AjnI CCWGG 2 cut(s) 158, 699
AleI CACNNNNGTG 1 cut(s) 11
AluBI AGCT 3 cut(s) 80, 196, 545
AluI AGCT 3 cut(s) 80, 196, 545
Alw21I GWGCWC 1 cut(s) 169
Alw26I GTCTC 1 cut(s) 268
AlwI GGATC 1 cut(s) 619
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 1 cut(s) 574
ApeKI GCWGC 2 cut(s) 118, 134
ApoI RAATTY 1 cut(s) 773
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 3 cut(s) 296, 693, 754
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 3 cut(s) 37, 262, 634
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 3 cut(s) 296, 693, 754
BaeGI GKGCMC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 2 cut(s) 105, 146
BciT130I CCWGG 2 cut(s) 160, 701
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 1 cut(s) 268
BfaI CTAG 2 cut(s) 672, 778
BfmI CTRYAG 1 cut(s) 765
BfoI RGCGCY 1 cut(s) 71
BglI GCCNNNNNGGC 1 cut(s) 53
BglII AGATCT 2 cut(s) 378, 654
BisI GCNGC 2 cut(s) 119, 135
BlsI GCNGC 2 cut(s) 120, 136
BmcAI AGTACT 1 cut(s) 735
Bme1390I CCNGG 3 cut(s) 49, 160, 701
Bme18I GGWCC 3 cut(s) 296, 693, 754
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 3 cut(s) 296, 693, 754
BmiI GGNNCC 1 cut(s) 694
BmrFI CCNGG 3 cut(s) 49, 160, 701
BmsI GCATC 2 cut(s) 163, 277
BpiI GAAGAC 1 cut(s) 714
BpmI CTGGAG 2 cut(s) 267, 666
BpuEI CTTGAG 4 cut(s) 203, 555, 569, 758
BpuMI CCSGG 1 cut(s) 49
BsaJI CCNNGG 5 cut(s) 138, 177, 577, 699, 750
Bsc4I CCNNNNNNNGG 2 cut(s) 689, 767
Bse1I ACTGG 3 cut(s) 133, 250, 524
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 2 cut(s) 160, 701
BseDI CCNNGG 5 cut(s) 138, 177, 577, 699, 750
BseGI GGATG 3 cut(s) 25, 292, 413
BseLI CCNNNNNNNGG 2 cut(s) 689, 767
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 3 cut(s) 133, 250, 524
BseRI GAGGAG 1 cut(s) 51
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 2 cut(s) 105, 146
BsgI GTGCAG 1 cut(s) 153
BshFI GGCC 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 169
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 1 cut(s) 706
BslI CCNNNNNNNGG 2 cut(s) 689, 767
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 706
BsnI GGCC 1 cut(s) 576
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 2 cut(s) 8, 169
Bsp143I GATC 6 cut(s) 107, 235, 378, 539, 611, 654
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 1 cut(s) 62
BspANI GGCC 1 cut(s) 576
BspCNI CTCAG 1 cut(s) 503
BspLI GGNNCC 1 cut(s) 694
BspPI GGATC 1 cut(s) 619
BsrDI GCAATG 1 cut(s) 417
BsrI ACTGG 3 cut(s) 133, 250, 524
BssECI CCNNGG 5 cut(s) 138, 177, 577, 699, 750
BssMI GATC 6 cut(s) 107, 235, 378, 539, 611, 654
BssT1I CCWWGG 4 cut(s) 138, 177, 577, 750
Bst2UI CCWGG 2 cut(s) 160, 701
Bst4CI ACNGT 3 cut(s) 307, 448, 603
Bst6I CTCTTC 2 cut(s) 68, 376
BstAPI GCANNNNNTGC 1 cut(s) 595
BstC8I GCNNGC 4 cut(s) 54, 123, 198, 628
BstDEI CTNAG 1 cut(s) 490
BstDSI CCRYGG 1 cut(s) 577
BstF5I GGATG 3 cut(s) 25, 292, 413
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 6 cut(s) 110, 238, 381, 542, 614, 657
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 6 cut(s) 107, 235, 378, 539, 611, 654
BstMWI GCNNNNNNNGC 5 cut(s) 53, 131, 164, 173, 595
BstNI CCWGG 2 cut(s) 160, 701
BstSCI CCNGG 3 cut(s) 47, 158, 699
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 105, 146
BstV2I GAAGAC 1 cut(s) 714
BstX2I RGATCY 2 cut(s) 378, 654
BstXI CCANNNNNNTGG 1 cut(s) 159
BstYI RGATCY 2 cut(s) 378, 654
BsuRI GGCC 1 cut(s) 576
BtgI CCRYGG 1 cut(s) 577
BtsCI GGATG 3 cut(s) 25, 292, 413
BtsIMutI CAGTG 2 cut(s) 126, 498
Cac8I GCNNGC 4 cut(s) 54, 123, 198, 628
CfoI GCGC 1 cut(s) 70
Cfr13I GGNCC 3 cut(s) 296, 693, 754
Csp6I GTAC 2 cut(s) 308, 734
CviAII CATG 3 cut(s) 404, 433, 578
CviQI GTAC 2 cut(s) 308, 734
DdeI CTNAG 1 cut(s) 490
DpnI GATC 6 cut(s) 109, 237, 380, 541, 613, 656
DpnII GATC 6 cut(s) 107, 235, 378, 539, 611, 654
DraIII CACNNNGTG 1 cut(s) 493
Eam1104I CTCTTC 2 cut(s) 68, 376
EarI CTCTTC 2 cut(s) 68, 376
Eco130I CCWWGG 4 cut(s) 138, 177, 577, 750
Eco47I GGWCC 3 cut(s) 296, 693, 754
Eco57I CTGAAG 2 cut(s) 92, 735
Eco88I CYCGRG 1 cut(s) 326
EcoO109I RGGNCCY 2 cut(s) 693, 754
EcoRII CCWGG 2 cut(s) 158, 699
EcoT14I CCWWGG 4 cut(s) 138, 177, 577, 750
ErhI CCWWGG 4 cut(s) 138, 177, 577, 750
FaeI CATG 3 cut(s) 407, 436, 581
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 1 cut(s) 706
FatI CATG 3 cut(s) 403, 432, 577
Fnu4HI GCNGC 2 cut(s) 119, 135
FokI GGATG 3 cut(s) 32, 299, 400
Fsp4HI GCNGC 2 cut(s) 119, 135
FspBI CTAG 2 cut(s) 672, 778
GlaI GCGC 1 cut(s) 69
GluI GCNGC 2 cut(s) 119, 135
GsuI CTGGAG 2 cut(s) 267, 666
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 1 cut(s) 576
HapII CCGG 1 cut(s) 48
HhaI GCGC 1 cut(s) 70
Hin1II CATG 3 cut(s) 407, 436, 581
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 513, 582
HpaII CCGG 1 cut(s) 48
HphI GGTGA 3 cut(s) 37, 262, 634
Hpy166II GTNNAC 2 cut(s) 487, 521
Hpy188I TCNGA 2 cut(s) 35, 112
Hpy188III TCNNGA 4 cut(s) 170, 220, 239, 257
Hpy8I GTNNAC 2 cut(s) 487, 521
HpyAV CCTTC 3 cut(s) 107, 401, 615
HpyCH4III ACNGT 3 cut(s) 307, 448, 603
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 2 cut(s) 134, 290
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 131, 164, 173, 595
HpyF3I CTNAG 1 cut(s) 490
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 3 cut(s) 407, 436, 581
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 6 cut(s) 107, 235, 378, 539, 611, 654
Lsp1109I GCAGC 2 cut(s) 105, 146
LweI GCATC 2 cut(s) 163, 277
MaeI CTAG 2 cut(s) 672, 778
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 3 cut(s) 229, 476, 498
MalI GATC 6 cut(s) 109, 237, 380, 541, 613, 656
MboI GATC 6 cut(s) 107, 235, 378, 539, 611, 654
MboII GAAGA 6 cut(s) 85, 363, 419, 658, 719, 728
MflI RGATCY 2 cut(s) 378, 654
MhlI GDGCHC 2 cut(s) 8, 169
MluCI AATT 4 cut(s) 223, 344, 742, 773
MlyI GAGTC 2 cut(s) 507, 576
MmeI TCCRAC 3 cut(s) 13, 81, 274
MnlI CCTC 9 cut(s) 10, 29, 32, 223, 322, 579, 650, 706, 767
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 1 cut(s) 11
MspA1I CMGCKG 2 cut(s) 62, 545
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 3 cut(s) 49, 160, 701
MvaI CCWGG 2 cut(s) 160, 701
MwoI GCNNNNNNNGC 5 cut(s) 53, 131, 164, 173, 595
NciI CCSGG 1 cut(s) 49
NcoI CCATGG 1 cut(s) 577
NdeII GATC 6 cut(s) 107, 235, 378, 539, 611, 654
NlaIII CATG 3 cut(s) 407, 436, 581
NlaIV GGNNCC 1 cut(s) 694
NmuCI GTSAC 2 cut(s) 476, 498
OliI CACNNNNGTG 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 326
PkrI GCNGC 2 cut(s) 120, 136
PleI GAGTC 2 cut(s) 507, 576
PpsI GAGTC 2 cut(s) 507, 576
PpuMI RGGWCCY 2 cut(s) 693, 754
Psp5II RGGWCCY 2 cut(s) 693, 754
Psp6I CCWGG 2 cut(s) 158, 699
PspGI CCWGG 2 cut(s) 158, 699
PspN4I GGNNCC 1 cut(s) 694
PspPI GGNCC 3 cut(s) 296, 693, 754
PspPPI RGGWCCY 2 cut(s) 693, 754
PspXI VCTCGAGB 1 cut(s) 326
PsuI RGATCY 2 cut(s) 378, 654
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 309, 735
RsaNI GTAC 2 cut(s) 308, 734
RseI CAYNNNNRTG 1 cut(s) 11
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 119, 135
Sau3AI GATC 6 cut(s) 107, 235, 378, 539, 611, 654
Sau96I GGNCC 3 cut(s) 296, 693, 754
ScaI AGTACT 1 cut(s) 735
SchI GAGTC 2 cut(s) 507, 576
ScrFI CCNGG 3 cut(s) 49, 160, 701
SduI GDGCHC 2 cut(s) 8, 169
SfaNI GCATC 2 cut(s) 163, 277
SfcI CTRYAG 1 cut(s) 765
Sfr274I CTCGAG 1 cut(s) 326
SinI GGWCC 3 cut(s) 296, 693, 754
SlaI CTCGAG 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 11
SmlI CTYRAG 5 cut(s) 218, 326, 570, 584, 737
SmoI CTYRAG 5 cut(s) 218, 326, 570, 584, 737
Sse9I AATT 4 cut(s) 223, 344, 742, 773
SsiI CCGC 1 cut(s) 62
SspMI CTAG 2 cut(s) 672, 778
StyD4I CCNGG 3 cut(s) 47, 158, 699
StyI CCWWGG 4 cut(s) 138, 177, 577, 750
TaaI ACNGT 3 cut(s) 307, 448, 603
TaiI ACGT 1 cut(s) 597
TaqI TCGA 5 cut(s) 171, 313, 327, 390, 538
TaqII GACCGA 1 cut(s) 313
TasI AATT 4 cut(s) 223, 344, 742, 773
TatI WGTACW 2 cut(s) 307, 733
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 2 cut(s) 133, 498
TseFI GTSAC 2 cut(s) 476, 498
TseI GCWGC 2 cut(s) 118, 134
Tsp45I GTSAC 2 cut(s) 476, 498
TspDTI ATGAA 4 cut(s) 344, 357, 402, 420
TspGWI ACGGA 2 cut(s) 353, 506
TspRI CASTG 2 cut(s) 133, 498
VpaK11BI GGWCC 3 cut(s) 296, 693, 754
XapI RAATTY 1 cut(s) 773
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XhoI CTCGAG 1 cut(s) 326
XspI CTAG 2 cut(s) 672, 778
ZrmI AGTACT 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.