Rroxscaffold_2G00116470

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001836
Physical Location & Seq
Forward (+)
43739081 .. 43744227
5147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116470.1

Sequence Viewer

Length: 1572 bp
ATGTTCAAGTCGAAGCTGCAGGAGCTGTGCCAGAAGAAGGCGTGGCCGTTACCCGAGTACAGCAGCAACAAGGAGGGCCTCGATCACGACCCTCGTTTCTCAGCCACCGTCAAAATCAACGGCCTCGAATTCAACACTGTCGACTCCTTCAGATCCTCCAAGTTGGCCCAAAACGACGCCGCTAGGCTCGCCTTCGATCACTTCTCTGCCCCCGCTACCCGCCCCATTCCTTTCGATTTCCCTCAGCCGAGTCTTGTCGCTATCACTCCCACTCCCTCAGGATTTCCCACAGCACTGGGCACTGATCAGAGTTTTACAACCACACCACTGCAATTGCAAAAGACTCAAGAAACACATCAACCTCCTCAGGTCAGTGGTAATGCAACAGATGGTGGTTCCCAGGTCAGTGGTAATGCAACAGTTGATGGTTCTCAGGTCAGTGGTAATGCAACAGTTGCCGGTTTTAAGGTCAGTGGTAATGCAACAGTTGCCGGTTCTCAGGTCAGTGCAACAGGTTCCAGTTCTCAGATCAGTGGCGGAGCATTAGGTGTTGAAGGTGATAATAGATCTAAAGATATACAACATTTGCATAAGAACCAGCTGCAGCGTTATGCTCATAAGAGGAATCTCATTTTACCTGTCTACTCTTGTGAGCGGAAAGGTCCTCCTCATGCCGCTCGTTTCAAATGTACAGTAACAATCAATGGAAAAACTTATCAGGGTCAAGAATTTCTTCCCACTGTGAAGGATGCTGAACATGCAGCTGCGGAAGTTGCTTTGATCTCCCTGTTGCCAAATGGAGTCCAAGAAGATGATTCCGGTTTGTACAAGAACCTTTTACAAGAACTAATTCAAAAAGAAGGTCAACATGTACCACTCTATAGCACAAGGACATCCGGTGAACCTCACATGCTTACTTTTGTTTCAACCGTGGAGATTGGAGGAGAATCTTTTACCGGTCAAGTAGCAAGAAGCAAGAAGCTGGCAGAAGTGAGTGCAGCAAAGGTTGCCTATAATACTTTGAGAGATCGTAAGTCAAGTCCGATCCCATTGTCTCTGACCCAGGATTCACAAGGGCAAGAAGGATTGCAGTTCTTATCTTCGAATGCTCCATCATACATGGCTCCTGATCTACAACAAGTGAGACCTAATGCTCCCAAGATTTCAATTCCAAATTCAGTTACTAGGGAACAAGAAAAGGAGATCAGCGTGGCTGCTGTGGGAACTAGTCATCAGTCTACAACTGTATCTTCAGGAGTTGATAATCATACAGTAGGTGGATCCACTTCGTCTGGCACTGTACCTGGCCGTGTACCGAAGACTTTACGTTCTGCTTCCCCTGCATCCCAAAGTGGATCCTCTTCGACTTTGACTTATTTCTGTACTTCAAATATGGACTCTACTCTTCAGCCACCAGCGAAAAGAACTACATCATACTCTAACATAGTTGCTGTACATCCACGGATGTTGAATATGACACTCCCAGAAGACTGCGTTGTGTTGCCCCTTAGTGATGAAACTTGGGTTGCTATATCACCCATGAAGACAGAAGGTATAACAAATCACTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0098586 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

523

Amino Acids

55.82

Weight (kDa)

7.71

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 3 - 65 9.4e-12 Double-stranded RNA binding motif
dsrm PF00035 198 - 262 2.2e-14 Double-stranded RNA binding motif
dsrm PF00035 276 - 341 1.2e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 655, 677
AccI GTMKAC 3 cut(s) 141, 642, 1238
AciI CCGC 7 cut(s) 180, 213, 220, 537, 655, 675, 767
AclWI GGATC 6 cut(s) 147, 1039, 1275, 1288, 1350, 1363
AcoI YGGCCR 2 cut(s) 44, 1306
AcsI RAATTY 3 cut(s) 128, 728, 1174
AcuI CTGAAG 3 cut(s) 133, 1236, 1391
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 8 cut(s) 59, 691, 827, 873, 1302, 1314, 1384, 1455
AfiI CCNNNNNNNGG 1 cut(s) 37
AflIII ACRYGT 1 cut(s) 868
AgeI ACCGGT 1 cut(s) 956
AgsI TTSAA 9 cut(s) 7, 133, 554, 685, 854, 927, 1167, 1389, 1471
AhlI ACTAGT 1 cut(s) 1226
AjnI CCWGG 3 cut(s) 399, 1062, 1303
AluBI AGCT 5 cut(s) 16, 25, 601, 764, 982
AluI AGCT 5 cut(s) 16, 25, 601, 764, 982
Alw26I GTCTC 2 cut(s) 1059, 1138
AlwI GGATC 6 cut(s) 147, 1039, 1275, 1288, 1350, 1363
AlwNI CAGNNNCTG 1 cut(s) 25
Ama87I CYCGRG 1 cut(s) 53
AoxI GGCC 5 cut(s) 44, 76, 121, 165, 1306
ApeKI GCWGC 8 cut(s) 16, 63, 601, 604, 761, 764, 998, 1214
ApoI RAATTY 3 cut(s) 128, 728, 1174
ArsI GACNNNNNNTTYG 2 cut(s) 847, 879
AsiGI ACCGGT 1 cut(s) 956
Asp700I GAANNNNTTC 2 cut(s) 732, 849
AspS9I GGNCC 3 cut(s) 76, 166, 662
AsuHPI GGTGA 3 cut(s) 569, 911, 1527
AsuII TTCGAA 1 cut(s) 1103
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 662
AxyI CCTNAGG 2 cut(s) 277, 366
BaeGI GKGCMC 1 cut(s) 302
BaeI ACNNNNGTAYC 2 cut(s) 1230, 1263
BamHI GGATCC 2 cut(s) 1280, 1355
BbsI GAAGAC 3 cut(s) 1325, 1494, 1550
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 8 cut(s) 3, 75, 588, 616, 751, 773, 1010, 1201
BccI CCATC 3 cut(s) 383, 419, 1120
BceAI ACGGC 3 cut(s) 31, 136, 1293
BciT130I CCWGG 3 cut(s) 401, 1064, 1305
BclI TGATCA 1 cut(s) 304
BcoDI GTCTC 2 cut(s) 1059, 1138
BcuI ACTAGT 1 cut(s) 1226
BfaI CTAG 3 cut(s) 183, 1185, 1227
BfmI CTRYAG 3 cut(s) 17, 602, 880
BglII AGATCT 1 cut(s) 566
Bme1390I CCNGG 3 cut(s) 401, 1064, 1305
Bme18I GGWCC 1 cut(s) 662
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 3 cut(s) 76, 166, 662
BmiI GGNNCC 5 cut(s) 397, 517, 1125, 1282, 1357
BmrFI CCNGG 3 cut(s) 401, 1064, 1305
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 2 cut(s) 739, 1352
BmuI ACTGGG 1 cut(s) 305
BpiI GAAGAC 3 cut(s) 1325, 1494, 1550
Bpu10I CCTNAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 1103
BpuEI CTTGAG 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 1138
BsaJI CCNNGG 4 cut(s) 399, 930, 1062, 1460
BsaWI WCCGGW 3 cut(s) 818, 896, 956
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 37
Bse118I RCCGGY 3 cut(s) 458, 491, 956
Bse1I ACTGG 2 cut(s) 300, 519
Bse21I CCTNAGG 2 cut(s) 277, 366
BseBI CCWGG 3 cut(s) 401, 1064, 1305
BseDI CCNNGG 4 cut(s) 399, 930, 1062, 1460
BseGI GGATG 5 cut(s) 754, 893, 1343, 1456, 1470
BseLI CCNNNNNNNGG 1 cut(s) 37
BseMII CTCAG 7 cut(s) 114, 257, 291, 380, 446, 512, 539
BseNI ACTGG 2 cut(s) 300, 519
BseRI GAGGAG 3 cut(s) 354, 657, 957
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 8 cut(s) 3, 75, 588, 616, 751, 773, 1010, 1201
BsgI GTGCAG 1 cut(s) 1017
BshFI GGCC 5 cut(s) 46, 78, 123, 167, 1308
BshTI ACCGGT 1 cut(s) 956
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 5 cut(s) 459, 492, 819, 897, 957
BslI CCNNNNNNNGG 1 cut(s) 37
BsmAI GTCTC 2 cut(s) 1059, 1138
BsmI GAATGC 1 cut(s) 1111
BsnI GGCC 5 cut(s) 46, 78, 123, 167, 1308
Bso31I GGTCTC 1 cut(s) 1138
BsoBI CYCGRG 1 cut(s) 53
Bsp119I TTCGAA 1 cut(s) 1103
Bsp1286I GDGCHC 1 cut(s) 302
Bsp1407I TGTACA 3 cut(s) 689, 825, 1453
BspACI CCGC 7 cut(s) 180, 213, 220, 537, 655, 675, 767
BspANI GGCC 5 cut(s) 46, 78, 123, 167, 1308
BspCNI CTCAG 7 cut(s) 113, 256, 290, 379, 445, 511, 538
BspLI GGNNCC 5 cut(s) 397, 517, 1125, 1282, 1357
BspMAI CTGCAG 2 cut(s) 21, 606
BspPI GGATC 6 cut(s) 147, 1039, 1275, 1288, 1350, 1363
BspT104I TTCGAA 1 cut(s) 1103
BspTNI GGTCTC 1 cut(s) 1138
BsrBI CCGCTC 2 cut(s) 655, 677
BsrFI RCCGGY 3 cut(s) 458, 491, 956
BsrGI TGTACA 3 cut(s) 689, 825, 1453
BsrI ACTGG 2 cut(s) 300, 519
BssAI RCCGGY 3 cut(s) 458, 491, 956
BssECI CCNNGG 4 cut(s) 399, 930, 1062, 1460
BssNI GRCGYC 1 cut(s) 177
Bst2UI CCWGG 3 cut(s) 401, 1064, 1305
Bst6I CTCTTC 2 cut(s) 1366, 1410
BstACI GRCGYC 1 cut(s) 177
BstAPI GCANNNNNTGC 3 cut(s) 455, 488, 1007
BstAUI TGTACA 3 cut(s) 689, 825, 1453
BstBI TTCGAA 1 cut(s) 1103
BstC8I GCNNGC 2 cut(s) 189, 984
BstDEI CTNAG 8 cut(s) 100, 243, 277, 366, 432, 498, 525, 1508
BstDSI CCRYGG 2 cut(s) 930, 1460
BstF5I GGATG 5 cut(s) 754, 893, 1343, 1456, 1470
BstMAI GTCTC 2 cut(s) 1059, 1138
BstMWI GCNNNNNNNGC 8 cut(s) 22, 188, 455, 488, 758, 773, 1007, 1340
BstNI CCWGG 3 cut(s) 401, 1064, 1305
BstNSI RCATGY 3 cut(s) 761, 872, 913
BstSCI CCNGG 3 cut(s) 399, 1062, 1303
BstSFI CTRYAG 3 cut(s) 17, 602, 880
BstSLI GKGCMC 1 cut(s) 302
BstV1I GCAGC 8 cut(s) 3, 75, 588, 616, 751, 773, 1010, 1201
BstV2I GAAGAC 3 cut(s) 1325, 1494, 1550
BstX2I RGATCY 4 cut(s) 152, 566, 1280, 1355
BstXI CCANNNNNNTGG 2 cut(s) 295, 407
BstYI RGATCY 4 cut(s) 152, 566, 1280, 1355
Bsu36I CCTNAGG 2 cut(s) 277, 366
BsuRI GGCC 5 cut(s) 46, 78, 123, 167, 1308
BtgI CCRYGG 2 cut(s) 930, 1460
BtsCI GGATG 5 cut(s) 754, 893, 1343, 1456, 1470
BtsI GCAGTG 1 cut(s) 326
Cac8I GCNNGC 2 cut(s) 189, 984
CaiI CAGNNNCTG 1 cut(s) 25
Cfr10I RCCGGY 3 cut(s) 458, 491, 956
Cfr13I GGNCC 3 cut(s) 76, 166, 662
CseI GACGC 1 cut(s) 185
Csp6I GTAC 8 cut(s) 58, 690, 826, 872, 1301, 1313, 1383, 1454
CspAI ACCGGT 1 cut(s) 956
CspCI CAANNNNNGTGG 4 cut(s) 315, 350, 1449, 1484
CviAII CATG 6 cut(s) 671, 758, 869, 910, 1120, 1540
CviQI GTAC 8 cut(s) 58, 690, 826, 872, 1301, 1313, 1383, 1454
DdeI CTNAG 8 cut(s) 100, 243, 277, 366, 432, 498, 525, 1508
EaeI YGGCCR 2 cut(s) 44, 1306
Eam1104I CTCTTC 2 cut(s) 1366, 1410
EarI CTCTTC 2 cut(s) 1366, 1410
EciI GGCGGA 1 cut(s) 552
Eco31I GGTCTC 1 cut(s) 1138
Eco47I GGWCC 1 cut(s) 662
Eco57I CTGAAG 3 cut(s) 133, 1236, 1391
Eco81I CCTNAGG 2 cut(s) 277, 366
Eco88I CYCGRG 1 cut(s) 53
EcoO109I RGGNCCY 2 cut(s) 76, 662
EcoRI GAATTC 1 cut(s) 128
EcoRII CCWGG 3 cut(s) 399, 1062, 1303
FaeI CATG 6 cut(s) 674, 761, 872, 913, 1123, 1543
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FatI CATG 6 cut(s) 670, 757, 868, 909, 1119, 1539
FauI CCCGC 2 cut(s) 220, 227
FbaI TGATCA 1 cut(s) 304
FblI GTMKAC 3 cut(s) 141, 642, 1238
FokI GGATG 5 cut(s) 761, 880, 1330, 1443, 1477
FspBI CTAG 3 cut(s) 183, 1185, 1227
HaeIII GGCC 5 cut(s) 46, 78, 123, 167, 1308
HapII CCGG 5 cut(s) 459, 492, 819, 897, 957
HgaI GACGC 1 cut(s) 185
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 6 cut(s) 674, 761, 872, 913, 1123, 1543
HincII GTYRAC 2 cut(s) 142, 866
HindII GTYRAC 2 cut(s) 142, 866
HinfI GANTC 9 cut(s) 143, 250, 343, 625, 801, 815, 947, 1067, 1397
HpaII CCGG 5 cut(s) 459, 492, 819, 897, 957
HphI GGTGA 3 cut(s) 569, 911, 1527
Hpy166II GTNNAC 6 cut(s) 142, 643, 866, 902, 1239, 1313
Hpy188I TCNGA 5 cut(s) 152, 309, 528, 1044, 1059
Hpy188III TCNNGA 6 cut(s) 86, 279, 347, 725, 1127, 1254
Hpy8I GTNNAC 6 cut(s) 142, 643, 866, 902, 1239, 1313
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 8 cut(s) 31, 157, 202, 548, 739, 854, 1076, 1544
HpyCH4IV ACGT 1 cut(s) 1327
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 188, 455, 488, 758, 773, 1007, 1340
HpyF3I CTNAG 8 cut(s) 100, 243, 277, 366, 432, 498, 525, 1508
HpySE526I ACGT 1 cut(s) 1327
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 6 cut(s) 674, 761, 872, 913, 1123, 1543
Ksp22I TGATCA 1 cut(s) 304
LmnI GCTCC 5 cut(s) 22, 539, 1114, 1129, 1159
Lsp1109I GCAGC 8 cut(s) 3, 75, 588, 616, 751, 773, 1010, 1201
LweI GCATC 2 cut(s) 739, 1352
MaeI CTAG 3 cut(s) 183, 1185, 1227
MaeII ACGT 1 cut(s) 1327
MaeIII GTNAC 3 cut(s) 48, 694, 1180
MbiI CCGCTC 2 cut(s) 655, 677
MfeI CAATTG 1 cut(s) 332
MflI RGATCY 4 cut(s) 152, 566, 1280, 1355
MhlI GDGCHC 1 cut(s) 302
MluCI AATT 6 cut(s) 128, 332, 728, 849, 1167, 1174
MlyI GAGTC 5 cut(s) 137, 259, 337, 810, 1391
MroXI GAANNNNTTC 2 cut(s) 732, 849
MseI TTAA 1 cut(s) 465
MspA1I CMGCKG 2 cut(s) 601, 764
MspI CCGG 5 cut(s) 459, 492, 819, 897, 957
MspR9I CCNGG 3 cut(s) 401, 1064, 1305
MunI CAATTG 1 cut(s) 332
Mva1269I GAATGC 1 cut(s) 1111
MvaI CCWGG 3 cut(s) 401, 1064, 1305
MwoI GCNNNNNNNGC 8 cut(s) 22, 188, 455, 488, 758, 773, 1007, 1340
NlaIII CATG 6 cut(s) 674, 761, 872, 913, 1123, 1543
NlaIV GGNNCC 5 cut(s) 397, 517, 1125, 1282, 1357
NmeAIII GCCGAG 1 cut(s) 273
NspI RCATGY 3 cut(s) 761, 872, 913
NspV TTCGAA 1 cut(s) 1103
PciI ACATGT 1 cut(s) 868
PctI GAATGC 1 cut(s) 1111
PdmI GAANNNNTTC 2 cut(s) 732, 849
PfeI GAWTC 4 cut(s) 625, 815, 947, 1067
PinAI ACCGGT 1 cut(s) 956
PleI GAGTC 5 cut(s) 137, 258, 337, 809, 1391
PpsI GAGTC 5 cut(s) 137, 258, 337, 809, 1391
PpuMI RGGWCCY 1 cut(s) 662
PscI ACATGT 1 cut(s) 868
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 3 cut(s) 399, 1062, 1303
PspGI CCWGG 3 cut(s) 399, 1062, 1303
PspN4I GGNNCC 5 cut(s) 397, 517, 1125, 1282, 1357
PspPI GGNCC 3 cut(s) 76, 166, 662
PspPPI RGGWCCY 1 cut(s) 662
PstI CTGCAG 2 cut(s) 21, 606
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 4 cut(s) 152, 566, 1280, 1355
PvuII CAGCTG 2 cut(s) 601, 764
RsaI GTAC 8 cut(s) 59, 691, 827, 873, 1302, 1314, 1384, 1455
RsaNI GTAC 8 cut(s) 58, 690, 826, 872, 1301, 1313, 1383, 1454
SalI GTCGAC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 465
Sau96I GGNCC 3 cut(s) 76, 166, 662
SchI GAGTC 5 cut(s) 137, 259, 337, 810, 1391
ScrFI CCNGG 3 cut(s) 401, 1064, 1305
SduI GDGCHC 1 cut(s) 302
SfaNI GCATC 2 cut(s) 739, 1352
SfcI CTRYAG 3 cut(s) 17, 602, 880
SfuI TTCGAA 1 cut(s) 1103
SinI GGWCC 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
SpeI ACTAGT 1 cut(s) 1226
Sse9I AATT 6 cut(s) 128, 332, 728, 849, 1167, 1174
SsiI CCGC 7 cut(s) 180, 213, 220, 537, 655, 675, 767
SspMI CTAG 3 cut(s) 183, 1185, 1227
StyD4I CCNGG 3 cut(s) 399, 1062, 1303
TaiI ACGT 1 cut(s) 1330
TaqI TCGA 8 cut(s) 11, 81, 126, 141, 195, 234, 1103, 1364
TasI AATT 6 cut(s) 128, 332, 728, 849, 1167, 1174
TatI WGTACW 5 cut(s) 57, 689, 825, 1382, 1453
TauI GCSGC 2 cut(s) 182, 677
TfiI GAWTC 4 cut(s) 625, 815, 947, 1067
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TseI GCWGC 8 cut(s) 16, 63, 601, 604, 761, 764, 998, 1214
TspDTI ATGAA 2 cut(s) 1530, 1556
TspGWI ACGGA 1 cut(s) 1477
VpaK11BI GGWCC 1 cut(s) 662
XapI RAATTY 3 cut(s) 128, 728, 1174
XceI RCATGY 3 cut(s) 761, 872, 913
XmiI GTMKAC 3 cut(s) 141, 642, 1238
XmnI GAANNNNTTC 2 cut(s) 732, 849
XspI CTAG 3 cut(s) 183, 1185, 1227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.