MD09G1243600.v1.1

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001836
Physical Location & Seq
Forward (+)
31121592 .. 31125489
3898 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1243600.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGGGTTCTGTTCCTTGGACTGCGGCCACTGATTCCGATACTACAGATGAAGCACGAGAGATAGAGATAGTGGAACCGGCCACTGATTCCGATACTACACATGAAGCACGAGAGATAGAGATAGTGGAGCCGAAGACTCAAGAAACGCATCAGATTCCTCAGGTGCCACTTCAGTTTAGTGGTAACGTATCAGGTGCTGCTTCTCAGGCGAATGCCGCCTTGTCGGGCAAGATGGATATGCAACATTTGTACAAGAATCAACTGCAAACTTATGCACAAAAGAGAAATCTGCTTCTACCTGTCTACTCTTGTGAGCGGGAAGGTCCACCACATAACTGTCGTTTCAAGTGTAGGGTAACTATTGATGGACAGACATATGAGGGTCAAGAGTTTCTTCCCACACTCAAGGATGCTGAACATGCAGTTGCAAAAGCTGCTTTAATGTCCCTTCTTCCAAATGGAGTTCAAGAGGATGATTCTGGTTTGTTCAAAAACCTTTTACAAGAACTGATTCAGAAGGAAGGTTTTTCTATACCAATCTATAGCTCAAGGATGTCTGGTGAACCTCACATTCCAGTTTTTGTTTCGTCTGTGGAGATAGAAGGAGAAACTTTTATAGGTCAAGAAGCAAGAAGCAAAAAGCAGGCAGAGTTGAGTGCAGCAAAGGTCGCTTACCATACCTTGAGAGAGCGTAAATCAAGTCAGCTACCTTTGGTCATGTCCTCTCGGAAAGGGCAAGAAGCTTCACAGATCTTATCTTCAAGTTTCCAGTCAAATCCAGCTTCTGATCAACATCAACAAGTCAGGCGTAATGCACCTGCGATCTTGTCTCCGAATACCAATACTAAAGAGAAGAAAAAGGAGAATAATGTAACTGCACAAGGAAAAAGTCAACACTCTACGGCAGGTGGGGAAGGATTCTCTTCCTCTTATGTTGTACATGGCCGTGCGCAGGAGATGAACAACGGTGCTAGCCCTGTGTCCCTAAACGGCTCCACTAATTGCATTATGGACTCTTCTCTTGAGCCACCAGCTAGAAGGAGTATGTATAACAAGGTCTCCGTTTACCCACGCAAGTCAAATATGGAATATCCTGAAGGTACCACTGTGTTGCCCATGAGCGATGAAACGTGGGTTGCTTGCTCACACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0098586 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

385

Amino Acids

42.23

Weight (kDa)

5.93

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 84 - 147 1.8e-13 Double-stranded RNA binding motif
dsrm PF00035 164 - 228 6.4e-14 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 826, 896
Acc16I TGCGCA 1 cut(s) 951
Acc36I ACCTGC 2 cut(s) 826, 896
Acc65I GGTACC 1 cut(s) 1100
AccB1I GGYRCC 2 cut(s) 163, 1100
AccBSI CCGCTC 1 cut(s) 316
AccI GTMKAC 1 cut(s) 303
AciI CCGC 3 cut(s) 23, 216, 316
AcoI YGGCCR 3 cut(s) 24, 78, 943
AcuI CTGAAG 2 cut(s) 155, 1116
AfaI GTAC 3 cut(s) 251, 939, 1102
AfiI CCNNNNNNNGG 1 cut(s) 952
AgsI TTSAA 4 cut(s) 346, 467, 490, 762
AluBI AGCT 6 cut(s) 434, 546, 706, 743, 782, 1034
AluI AGCT 6 cut(s) 434, 546, 706, 743, 782, 1034
Alw26I GTCTC 2 cut(s) 834, 1063
AlwNI CAGNNNCTG 2 cut(s) 197, 785
AoxI GGCC 3 cut(s) 24, 78, 943
ApeKI GCWGC 3 cut(s) 197, 434, 659
Asp700I GAANNNNTTC 1 cut(s) 510
Asp718I GGTACC 1 cut(s) 1100
AspLEI GCGC 1 cut(s) 952
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 572
AsuNHI GCTAGC 1 cut(s) 971
AvaII GGWCC 1 cut(s) 323
AxyI CCTNAGG 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 1092, 1125
BanI GGYRCC 2 cut(s) 163, 1100
BauI CACGAG 2 cut(s) 54, 108
BbsI GAAGAC 1 cut(s) 140
BbvI GCAGC 3 cut(s) 184, 421, 671
BccI CCATC 2 cut(s) 226, 359
BceAI ACGGC 3 cut(s) 918, 930, 1006
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 2 cut(s) 834, 1063
BfaI CTAG 2 cut(s) 972, 1035
BfmI CTRYAG 2 cut(s) 42, 541
BfuAI ACCTGC 2 cut(s) 826, 896
BglII AGATCT 1 cut(s) 750
BisI GCNGC 5 cut(s) 24, 198, 216, 435, 660
BlsI GCNGC 5 cut(s) 25, 199, 217, 436, 661
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 5 cut(s) 75, 129, 165, 994, 1102
BmsI GCATC 2 cut(s) 157, 400
BmtI GCTAGC 1 cut(s) 975
BpiI GAAGAC 1 cut(s) 140
BpuEI CTTGAG 5 cut(s) 123, 389, 532, 703, 1043
BsaBI GATNNNNATC 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 1063
BsaJI CCNNGG 1 cut(s) 14
BsaXI ACNNNNNCTCC 2 cut(s) 1043, 1073
Bsc4I CCNNNNNNNGG 1 cut(s) 952
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 575, 769
Bse21I CCTNAGG 1 cut(s) 159
Bse8I GATNNNNATC 1 cut(s) 792
BseDI CCNNGG 1 cut(s) 14
BseGI GGATG 3 cut(s) 415, 478, 558
BseJI GATNNNNATC 1 cut(s) 792
BseLI CCNNNNNNNGG 1 cut(s) 952
BseMII CTCAG 2 cut(s) 173, 218
BseNI ACTGG 2 cut(s) 575, 769
BseXI GCAGC 3 cut(s) 184, 421, 671
BsgI GTGCAG 2 cut(s) 678, 861
BshFI GGCC 3 cut(s) 26, 80, 945
BshNI GGYRCC 2 cut(s) 163, 1100
BsiSI CCGG 1 cut(s) 77
BslFI GGGAC 2 cut(s) 430, 967
BslI CCNNNNNNNGG 1 cut(s) 952
BsmAI GTCTC 2 cut(s) 834, 1063
BsmFI GGGAC 2 cut(s) 430, 967
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 3 cut(s) 26, 80, 945
Bso31I GGTCTC 1 cut(s) 1063
Bsp1407I TGTACA 2 cut(s) 249, 937
Bsp143I GATC 3 cut(s) 750, 787, 822
BspACI CCGC 3 cut(s) 23, 216, 316
BspANI GGCC 3 cut(s) 26, 80, 945
BspCNI CTCAG 2 cut(s) 172, 217
BspLI GGNNCC 5 cut(s) 75, 129, 165, 994, 1102
BspMI ACCTGC 2 cut(s) 826, 896
BspOI GCTAGC 1 cut(s) 975
BspT107I GGYRCC 2 cut(s) 163, 1100
BspTNI GGTCTC 1 cut(s) 1063
BsrBI CCGCTC 1 cut(s) 316
BsrFI RCCGGY 1 cut(s) 76
BsrGI TGTACA 2 cut(s) 249, 937
BsrI ACTGG 2 cut(s) 575, 769
BssAI RCCGGY 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 14
BssMI GATC 3 cut(s) 750, 787, 822
BssSI CACGAG 2 cut(s) 54, 108
BssT1I CCWWGG 1 cut(s) 14
Bst2BI CACGAG 2 cut(s) 54, 108
Bst4CI ACNGT 3 cut(s) 338, 968, 1108
Bst6I CTCTTC 2 cut(s) 928, 1021
BstAPI GCANNNNNTGC 1 cut(s) 434
BstAUI TGTACA 2 cut(s) 249, 937
BstC8I GCNNGC 3 cut(s) 645, 973, 1141
BstDEI CTNAG 2 cut(s) 159, 204
BstF5I GGATG 3 cut(s) 415, 478, 558
BstHHI GCGC 1 cut(s) 952
BstKTI GATC 3 cut(s) 753, 790, 825
BstMAI GTCTC 2 cut(s) 834, 1063
BstMBI GATC 3 cut(s) 750, 787, 822
BstMWI GCNNNNNNNGC 5 cut(s) 206, 215, 419, 434, 668
BstNSI RCATGY 1 cut(s) 422
BstSFI CTRYAG 2 cut(s) 42, 541
BstV1I GCAGC 3 cut(s) 184, 421, 671
BstV2I GAAGAC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 750
BstYI RGATCY 1 cut(s) 750
Bsu36I CCTNAGG 1 cut(s) 159
BsuRI GGCC 3 cut(s) 26, 80, 945
BtgZI GCGATG 1 cut(s) 1137
BtsCI GGATG 3 cut(s) 415, 478, 558
BtsIMutI CAGTG 3 cut(s) 27, 81, 1104
BveI ACCTGC 2 cut(s) 826, 896
Cac8I GCNNGC 3 cut(s) 645, 973, 1141
CaiI CAGNNNCTG 2 cut(s) 197, 785
CfoI GCGC 1 cut(s) 952
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 3 cut(s) 250, 938, 1101
CviAII CATG 5 cut(s) 101, 419, 718, 941, 1117
CviQI GTAC 3 cut(s) 250, 938, 1101
DdeI CTNAG 2 cut(s) 159, 204
DpnI GATC 3 cut(s) 752, 789, 824
DpnII GATC 3 cut(s) 750, 787, 822
EaeI YGGCCR 3 cut(s) 24, 78, 943
Eam1104I CTCTTC 2 cut(s) 928, 1021
EarI CTCTTC 2 cut(s) 928, 1021
Eco130I CCWWGG 1 cut(s) 14
Eco31I GGTCTC 1 cut(s) 1063
Eco47I GGWCC 1 cut(s) 323
Eco57I CTGAAG 2 cut(s) 155, 1116
Eco81I CCTNAGG 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 14
ErhI CCWWGG 1 cut(s) 14
FaeI CATG 5 cut(s) 104, 422, 721, 944, 1120
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 2 cut(s) 430, 967
FatI CATG 5 cut(s) 100, 418, 717, 940, 1116
FauI CCCGC 1 cut(s) 309
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 1 cut(s) 787
FblI GTMKAC 1 cut(s) 303
Fnu4HI GCNGC 5 cut(s) 24, 198, 216, 435, 660
FokI GGATG 3 cut(s) 422, 485, 565
Fsp4HI GCNGC 5 cut(s) 24, 198, 216, 435, 660
FspBI CTAG 2 cut(s) 972, 1035
FspI TGCGCA 1 cut(s) 951
GlaI GCGC 1 cut(s) 951
GluI GCNGC 5 cut(s) 24, 198, 216, 435, 660
HaeIII GGCC 3 cut(s) 26, 80, 945
HapII CCGG 1 cut(s) 77
HhaI GCGC 1 cut(s) 952
Hin1II CATG 5 cut(s) 104, 422, 721, 944, 1120
Hin6I GCGC 1 cut(s) 950
HinP1I GCGC 1 cut(s) 950
HincII GTYRAC 1 cut(s) 893
HindII GTYRAC 1 cut(s) 893
HindIII AAGCTT 1 cut(s) 741
HinfI GANTC 9 cut(s) 32, 86, 136, 154, 256, 476, 511, 918, 1013
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 5 cut(s) 304, 326, 563, 893, 1066
Hpy188I TCNGA 7 cut(s) 37, 91, 153, 516, 729, 787, 834
Hpy188III TCNNGA 6 cut(s) 140, 386, 467, 623, 1022, 1094
Hpy8I GTNNAC 5 cut(s) 304, 326, 563, 893, 1066
HpyAV CCTTC 8 cut(s) 314, 458, 511, 515, 596, 908, 1032, 1091
HpyCH4III ACNGT 3 cut(s) 338, 968, 1108
HpyCH4IV ACGT 2 cut(s) 186, 1130
HpyCH4V TGCA 9 cut(s) 241, 265, 275, 422, 428, 659, 815, 878, 1005
HpyF10VI GCNNNNNNNGC 5 cut(s) 206, 215, 419, 434, 668
HpyF3I CTNAG 2 cut(s) 159, 204
HpySE526I ACGT 2 cut(s) 186, 1130
Hsp92II CATG 5 cut(s) 104, 422, 721, 944, 1120
HspAI GCGC 1 cut(s) 950
KpnI GGTACC 1 cut(s) 1104
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 3 cut(s) 750, 787, 822
LmnI GCTCC 2 cut(s) 127, 998
Lsp1109I GCAGC 3 cut(s) 184, 421, 671
LweI GCATC 2 cut(s) 157, 400
MaeI CTAG 2 cut(s) 972, 1035
MaeII ACGT 2 cut(s) 186, 1130
MaeIII GTNAC 3 cut(s) 182, 355, 871
MalI GATC 3 cut(s) 752, 789, 824
MbiI CCGCTC 1 cut(s) 316
MboI GATC 3 cut(s) 750, 787, 822
MboII GAAGA 7 cut(s) 145, 386, 443, 750, 865, 915, 1008
MflI RGATCY 1 cut(s) 750
MluCI AATT 1 cut(s) 1000
MlyI GAGTC 2 cut(s) 130, 1007
MnlI CCTC 6 cut(s) 168, 373, 463, 576, 733, 937
MroXI GAANNNNTTC 1 cut(s) 510
MseI TTAA 1 cut(s) 440
MslI CAYNNNNRTG 2 cut(s) 945, 1150
MspI CCGG 1 cut(s) 77
Mva1269I GAATGC 1 cut(s) 217
MwoI GCNNNNNNNGC 5 cut(s) 206, 215, 419, 434, 668
NdeI CATATG 1 cut(s) 376
NdeII GATC 3 cut(s) 750, 787, 822
NheI GCTAGC 1 cut(s) 971
NlaIII CATG 5 cut(s) 104, 422, 721, 944, 1120
NlaIV GGNNCC 5 cut(s) 75, 129, 165, 994, 1102
NsbI TGCGCA 1 cut(s) 951
NspI RCATGY 1 cut(s) 422
PaqCI CACCTGC 2 cut(s) 826, 896
PctI GAATGC 1 cut(s) 217
PdmI GAANNNNTTC 1 cut(s) 510
PfeI GAWTC 7 cut(s) 32, 86, 154, 256, 476, 511, 918
PkrI GCNGC 5 cut(s) 25, 199, 217, 436, 661
PleI GAGTC 2 cut(s) 130, 1007
PpsI GAGTC 2 cut(s) 130, 1007
PspN4I GGNNCC 5 cut(s) 75, 129, 165, 994, 1102
PspPI GGNCC 1 cut(s) 323
PstNI CAGNNNCTG 2 cut(s) 197, 785
PsuI RGATCY 1 cut(s) 750
RsaI GTAC 3 cut(s) 251, 939, 1102
RsaNI GTAC 3 cut(s) 250, 938, 1101
RseI CAYNNNNRTG 2 cut(s) 945, 1150
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 5 cut(s) 24, 198, 216, 435, 660
Sau3AI GATC 3 cut(s) 750, 787, 822
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 2 cut(s) 130, 1007
SfaNI GCATC 2 cut(s) 157, 400
SfcI CTRYAG 2 cut(s) 42, 541
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 2 cut(s) 945, 1150
SmlI CTYRAG 5 cut(s) 138, 404, 547, 682, 1022
SmoI CTYRAG 5 cut(s) 138, 404, 547, 682, 1022
Sse9I AATT 1 cut(s) 1000
SsiI CCGC 3 cut(s) 23, 216, 316
SspMI CTAG 2 cut(s) 972, 1035
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 3 cut(s) 338, 968, 1108
TaiI ACGT 2 cut(s) 189, 1133
TasI AATT 1 cut(s) 1000
TatI WGTACW 2 cut(s) 249, 937
TauI GCSGC 2 cut(s) 26, 218
TfiI GAWTC 7 cut(s) 32, 86, 154, 256, 476, 511, 918
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 3 cut(s) 34, 88, 1111
TseI GCWGC 3 cut(s) 197, 434, 659
TspDTI ATGAA 4 cut(s) 63, 117, 974, 1140
TspGWI ACGGA 1 cut(s) 1051
TspRI CASTG 3 cut(s) 34, 88, 1111
VpaK11BI GGWCC 1 cut(s) 323
XceI RCATGY 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 510
XspI CTAG 2 cut(s) 972, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.