Rorug02G0273600

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001836
Physical Location & Seq
Forward (+)
29250875 .. 29252063
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0273600.1

Sequence Viewer

Length: 684 bp
ATGGCATACTCCTTTCACTCCCAAGCTTTCTTCTGCTCAAGCGTTCTAATATTCAAGATTATCAGTCTTCACCAGTCAGCCTTGGCTTGGAGTTGGGGACCTGATGTTCCTCCTACACAGGGCTTTGTCTCCCTCCCTTTCAACCGATCCTACTACCATATTCAGAAACCTTATGATGTGCCCGAAGACCAGCGCTACTGCTTCATCGATGGAGTTCATAAATGTTGGGTCTACTCCACAGACAAACCTCACACTACTACCAGCCAAACCCTGCCTCGCACTGAGATTGCCATACAAGGATACAATTACTCTTCTGGGGTGTGGGAGTTTGAAGCATTTGGGTACGTCCCAAAAGGGACATCAGGGGTGTGCATAATGCAAGTGTTTGGAGCAAGTGCTCCTTATGCCACAACTCTGATGCTGAGGGTCTACAATGGTTCACTAACATACTACAAGACTCCGGTGTTGGTTCCCAACATCTACGATAAGTGGTTCAAGCTCAATGTGGTTCACGATGTTGATGCTGCAAAAGTCAGAGTGTACATTGATGGATGTCTCAAAATCGAAGCAGATGGTCGTGGAGGAACCTCTCATGCTTTCAAATGTGGAGTGTATGCCCAGATGAATGACTCTACTTACATGGAGTCTCGCTGGAAAGACATCAAAGTTTTAAGGAAGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0098586 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

227

Amino Acids

25.84

Weight (kDa)

8.58

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 50 - 223 5.5e-26 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 231, 429
AclWI GGATC 1 cut(s) 141
AfaI GTAC 2 cut(s) 344, 542
AfeI AGCGCT 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 87, 119
AgsI TTSAA 5 cut(s) 55, 142, 332, 496, 601
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 2 cut(s) 26, 499
AluI AGCT 2 cut(s) 26, 499
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 3 cut(s) 133, 560, 651
AlwI GGATC 1 cut(s) 141
Aor51HI AGCGCT 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 62
AvaII GGWCC 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 183
BbsI GAAGAC 2 cut(s) 59, 192
Bbv12I GWGCWC 1 cut(s) 400
BbvCI CCTCAGC 1 cut(s) 422
BbvI GCAGC 1 cut(s) 511
BccI CCATC 3 cut(s) 203, 542, 566
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 3 cut(s) 133, 560, 651
BfoI RGCGCY 1 cut(s) 196
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 3 cut(s) 99, 471, 586
BmsI GCATC 2 cut(s) 408, 511
BpiI GAAGAC 2 cut(s) 59, 192
Bpu10I CCTNAGC 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 119
Bse1I ACTGG 1 cut(s) 73
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 1 cut(s) 557
BseLI CCNNNNNNNGG 2 cut(s) 87, 119
BseMII CTCAG 2 cut(s) 273, 413
BseNI ACTGG 1 cut(s) 73
BseSI GKGCMC 1 cut(s) 183
BseXI GCAGC 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 3 cut(s) 111, 332, 370
BslI CCNNNNNNNGG 2 cut(s) 87, 119
BsmAI GTCTC 3 cut(s) 133, 560, 651
BsmFI GGGAC 3 cut(s) 111, 332, 370
Bsp1286I GDGCHC 2 cut(s) 183, 400
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 1 cut(s) 146
BspCNI CTCAG 2 cut(s) 274, 414
BspDI ATCGAT 1 cut(s) 207
BspLI GGNNCC 3 cut(s) 99, 471, 586
BspPI GGATC 1 cut(s) 141
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 81
Bst6I CTCTTC 1 cut(s) 316
BstAUI TGTACA 1 cut(s) 540
BstDEI CTNAG 2 cut(s) 282, 422
BstF5I GGATG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 1 cut(s) 149
BstMAI GTCTC 3 cut(s) 133, 560, 651
BstMBI GATC 1 cut(s) 146
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstSLI GKGCMC 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 2 cut(s) 59, 192
Bsu15I ATCGAT 1 cut(s) 207
BsuI GTATCC 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 207
BtsCI GGATG 1 cut(s) 557
BtsIMutI CAGTG 1 cut(s) 279
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 98
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 2 cut(s) 343, 541
CviAII CATG 2 cut(s) 593, 640
CviJI RGCY 6 cut(s) 26, 80, 86, 123, 264, 499
CviKI_1 RGCY 6 cut(s) 26, 80, 86, 123, 264, 499
CviQI GTAC 2 cut(s) 343, 541
DdeI CTNAG 2 cut(s) 282, 422
DpnI GATC 1 cut(s) 148
DpnII GATC 1 cut(s) 146
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 98
Eco47III AGCGCT 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 2 cut(s) 596, 643
FalI AAGNNNNNCTT 2 cut(s) 385, 417
FaqI GGGAC 3 cut(s) 111, 332, 370
FatI CATG 2 cut(s) 592, 639
FblI GTMKAC 2 cut(s) 231, 429
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 1 cut(s) 564
Fsp4HI GCNGC 1 cut(s) 525
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 196
HapII CCGG 1 cut(s) 461
HhaI GCGC 1 cut(s) 195
Hin1II CATG 2 cut(s) 596, 643
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 24
HinfI GANTC 3 cut(s) 457, 629, 644
HpaII CCGG 1 cut(s) 461
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 5 cut(s) 232, 430, 440, 511, 541
Hpy188I TCNGA 3 cut(s) 165, 417, 536
Hpy188III TCNNGA 2 cut(s) 55, 512
Hpy8I GTNNAC 5 cut(s) 232, 430, 440, 511, 541
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 372, 379, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 2 cut(s) 282, 422
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 2 cut(s) 596, 643
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 1 cut(s) 146
LmnI GCTCC 2 cut(s) 389, 403
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 2 cut(s) 408, 511
MaeII ACGT 1 cut(s) 345
MalI GATC 1 cut(s) 148
MboI GATC 1 cut(s) 146
MboII GAAGA 4 cut(s) 22, 59, 197, 303
MhlI GDGCHC 2 cut(s) 183, 400
MluCI AATT 1 cut(s) 304
MlyI GAGTC 3 cut(s) 451, 623, 653
MnlI CCTC 7 cut(s) 120, 143, 258, 285, 417, 575, 598
MseI TTAA 1 cut(s) 671
MspI CCGG 1 cut(s) 461
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeII GATC 1 cut(s) 146
NlaIII CATG 2 cut(s) 596, 643
NlaIV GGNNCC 3 cut(s) 99, 471, 586
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 3 cut(s) 451, 623, 652
PpsI GAGTC 3 cut(s) 451, 623, 652
PpuMI RGGWCCY 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 99, 471, 586
PspPI GGNCC 1 cut(s) 98
PspPPI RGGWCCY 1 cut(s) 98
RsaI GTAC 2 cut(s) 344, 542
RsaNI GTAC 2 cut(s) 343, 541
SaqAI TTAA 1 cut(s) 671
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 1 cut(s) 146
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 3 cut(s) 451, 623, 653
SduI GDGCHC 2 cut(s) 183, 400
SetI ASST 7 cut(s) 28, 103, 172, 250, 348, 501, 590
SfaNI GCATC 2 cut(s) 408, 511
SinI GGWCC 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 1 cut(s) 304
SspI AATATT 1 cut(s) 51
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 1 cut(s) 348
TaqI TCGA 2 cut(s) 207, 564
TasI AATT 1 cut(s) 304
TatI WGTACW 1 cut(s) 540
Tru1I TTAA 1 cut(s) 671
Tru9I TTAA 1 cut(s) 671
TscAI CASTG 1 cut(s) 286
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 3 cut(s) 193, 206, 638
TspRI CASTG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 98
XmiI GTMKAC 2 cut(s) 231, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.