Rorug02G0273500

Double-stranded RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001836
Physical Location & Seq
Forward (+)
29247177 .. 29249953
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0273500.1

Sequence Viewer

Length: 1080 bp
ATGGCAAAATCACCAGAAGAAGAACACCCTCAGAAGGTTTTTGGTTGGGCTGCCAGAGACTCTTCTGGGTCGCTTTCCCCCTTTCATTTCTCTAGAAGGGAAAATGCTGATGGTGATGTAACAATAAAAGTCCTCTATTGTGGGGTTTGCCATTCAGACTTGCATTTTGTCAAGAACGAATGGGGGATCACCAACTATCCTATCGTACCTGGGCATGAAATGGTTGGTGTTGTGACCAAAACTGGGAAAAGCGTGACAAAATTCAAAGAAGGTGATAGTGTAGGAGTTGGGGTTATTGTGGGATCCTGCAACACATGTGAGACTTGCCAGCAGGACTTGGAGAACTATTGCCCCAAAGTGATATTCACCTATAACTCCCACAATCATGACAAAACTAAAACTTATGGTGGTTATTCTGATATGATTGTTGTTGACCATCGCTTTGTGCTCCGCTTTCCTGATAACTTACCTCCCGATTCTGGTGCTCCACTTCTATGTGCTGGGATCACTGTGTATAGTCCAATGAAGTACTACGGCATGACAGAGCCTGGAAAGCATTTGGGCGTGGCAGGACTTGGTGGACTAGGTCATATTGCTGTCAAAATTGGTAAGGCATTTGGTTTGAAGGTTACTGTCATCAGTACTTCCCCAGCTAAGGAGGATGAGGCTATTAACAGACTTGGGGCTGATTCTTTTGTTGTTTCAACTGACCCTGCACAAGTGAAGGCAGCCATGGGTACCATGGATTACATAATTGACACAGTATCTGCAGTTCATGCCCTCGCTCCATTAATTGGTCTACTCAAGCTGAATGGGAAGCTGGTTACTGTGGGTTTGCCGAATAAGCCACTGGAGTTGCCTATATTTCCATTGGTTCTGGGGAGGAAGCTCGTTGGTGGAAGCGACATTGGAGGGATAAAAGAGACGCAGGAGATGCTTGACTTTTGTGCCAAACACAATATTACCTCCGAAATCGAGCTAATTAAAATGGACTACATTAACACAGCTATGGAGCGACTTGCCAAATCAGATGTCAGGTATCGGTTTGTGATCGATGTGGGGAACTCCTTTTCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0003725 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009616 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0035821 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044003 GO:0044237 GO:0044238 GO:0044403 GO:0044419 GO:0044424 GO:0044464 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052018 GO:0052249 GO:0060145 GO:0060255 GO:0065007 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:0098586 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

359

Amino Acids

38.93

Weight (kDa)

6.46

Isoelectric Point (pI)

27.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 37 - 154 1.2e-27 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 194 - 317 2.9e-20 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 737
AccB1I GGYRCC 1 cut(s) 737
AccB7I CCANNNNNTGG 1 cut(s) 794
AccI GTMKAC 1 cut(s) 799
AciI CCGC 1 cut(s) 451
AclWI GGATC 4 cut(s) 194, 297, 310, 512
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 4 cut(s) 207, 530, 643, 739
AfiI CCNNNNNNNGG 5 cut(s) 34, 243, 479, 655, 794
AflIII ACRYGT 1 cut(s) 314
AgsI TTSAA 3 cut(s) 265, 625, 705
AjnI CCWGG 2 cut(s) 208, 547
AjuI GAANNNNNNNTTGG 2 cut(s) 347, 379
AluBI AGCT 6 cut(s) 653, 808, 820, 889, 979, 1007
AluI AGCT 6 cut(s) 653, 808, 820, 889, 979, 1007
Alw21I GWGCWC 2 cut(s) 450, 487
Alw26I GTCTC 3 cut(s) 51, 314, 917
AlwI GGATC 4 cut(s) 194, 297, 310, 512
AlwNI CAGNNNCTG 2 cut(s) 548, 767
ApeKI GCWGC 2 cut(s) 50, 728
ApoI RAATTY 1 cut(s) 260
ArsI GACNNNNNNTTYG 6 cut(s) 149, 181, 425, 457, 1017, 1049
AseI ATTAAT 1 cut(s) 791
Asp718I GGTACC 1 cut(s) 737
AsuHPI GGTGA 5 cut(s) 3, 125, 181, 284, 358
BamHI GGATCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 737
Bbv12I GWGCWC 2 cut(s) 450, 487
BbvI GCAGC 2 cut(s) 37, 740
BccI CCATC 2 cut(s) 104, 444
BceAI ACGGC 1 cut(s) 550
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BciT130I CCWGG 2 cut(s) 210, 549
BcoDI GTCTC 3 cut(s) 51, 314, 917
BfaI CTAG 2 cut(s) 93, 584
BfmI CTRYAG 1 cut(s) 768
BisI GCNGC 2 cut(s) 51, 729
BlsI GCNGC 2 cut(s) 52, 730
BmcAI AGTACT 2 cut(s) 530, 643
Bme1390I CCNGG 2 cut(s) 210, 549
BmiI GGNNCC 2 cut(s) 304, 739
BmrFI CCNGG 2 cut(s) 210, 549
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 1 cut(s) 924
BmuI ACTGGG 1 cut(s) 252
BpmI CTGGAG 1 cut(s) 872
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 788
Bsa29I ATCGAT 1 cut(s) 1053
BsaJI CCNNGG 3 cut(s) 209, 732, 741
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 243, 479, 655, 794
Bse1I ACTGG 2 cut(s) 247, 855
BseBI CCWGG 2 cut(s) 210, 549
BseCI ATCGAT 1 cut(s) 1053
BseDI CCNNGG 3 cut(s) 209, 732, 741
BseGI GGATG 1 cut(s) 667
BseLI CCNNNNNNNGG 5 cut(s) 34, 243, 479, 655, 794
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 2 cut(s) 247, 855
BseXI GCAGC 2 cut(s) 37, 740
BseYI CCCAGC 2 cut(s) 500, 649
BsgI GTGCAG 1 cut(s) 699
BshNI GGYRCC 1 cut(s) 737
BshVI ATCGAT 1 cut(s) 1053
BsiHKAI GWGCWC 2 cut(s) 450, 487
BslI CCNNNNNNNGG 5 cut(s) 34, 243, 479, 655, 794
BsmAI GTCTC 3 cut(s) 51, 314, 917
BsmBI CGTCTC 1 cut(s) 917
Bsp1286I GDGCHC 2 cut(s) 450, 487
Bsp143I GATC 4 cut(s) 186, 302, 504, 1050
Bsp19I CCATGG 2 cut(s) 732, 741
BspACI CCGC 1 cut(s) 451
BspCNI CTCAG 1 cut(s) 43
BspDI ATCGAT 1 cut(s) 1053
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 304, 739
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 4 cut(s) 194, 297, 310, 512
BspT107I GGYRCC 1 cut(s) 737
BsrI ACTGG 2 cut(s) 247, 855
BssECI CCNNGG 3 cut(s) 209, 732, 741
BssMI GATC 4 cut(s) 186, 302, 504, 1050
BssT1I CCWWGG 2 cut(s) 732, 741
Bst2UI CCWGG 2 cut(s) 210, 549
Bst4CI ACNGT 4 cut(s) 511, 634, 763, 829
Bst6I CTCTTC 1 cut(s) 67
BstAPI GCANNNNNTGC 2 cut(s) 776, 934
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 3 cut(s) 30, 654, 1074
BstDSI CCRYGG 2 cut(s) 732, 741
BstF5I GGATG 1 cut(s) 667
BstKTI GATC 4 cut(s) 189, 305, 507, 1053
BstMAI GTCTC 3 cut(s) 51, 314, 917
BstMBI GATC 4 cut(s) 186, 302, 504, 1050
BstMWI GCNNNNNNNGC 4 cut(s) 553, 776, 844, 934
BstNI CCWGG 2 cut(s) 210, 549
BstNSI RCATGY 1 cut(s) 318
BstSCI CCNGG 2 cut(s) 208, 547
BstSFI CTRYAG 1 cut(s) 768
BstV1I GCAGC 2 cut(s) 37, 740
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
Bsu15I ATCGAT 1 cut(s) 1053
BsuTUI ATCGAT 1 cut(s) 1053
BtgI CCRYGG 2 cut(s) 732, 741
BtgZI GCGATG 1 cut(s) 422
BtsCI GGATG 1 cut(s) 667
BtsIMutI CAGTG 2 cut(s) 507, 848
Cac8I GCNNGC 1 cut(s) 329
CaiI CAGNNNCTG 2 cut(s) 548, 767
CciI TCATGA 1 cut(s) 385
ClaI ATCGAT 1 cut(s) 1053
CseI GACGC 1 cut(s) 934
Csp6I GTAC 4 cut(s) 206, 529, 642, 738
CspCI CAANNNNNGTGG 2 cut(s) 837, 872
CviAII CATG 7 cut(s) 215, 315, 386, 538, 733, 742, 776
CviQI GTAC 4 cut(s) 206, 529, 642, 738
DdeI CTNAG 3 cut(s) 30, 654, 1074
DpnI GATC 4 cut(s) 188, 304, 506, 1052
DpnII GATC 4 cut(s) 186, 302, 504, 1050
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Eco130I CCWWGG 2 cut(s) 732, 741
EcoRII CCWGG 2 cut(s) 208, 547
EcoT14I CCWWGG 2 cut(s) 732, 741
ErhI CCWWGG 2 cut(s) 732, 741
Esp3I CGTCTC 1 cut(s) 917
FaeI CATG 7 cut(s) 218, 318, 389, 541, 736, 745, 779
FatI CATG 7 cut(s) 214, 314, 385, 537, 732, 741, 775
FblI GTMKAC 1 cut(s) 799
Fnu4HI GCNGC 2 cut(s) 51, 729
FokI GGATG 1 cut(s) 674
Fsp4HI GCNGC 2 cut(s) 51, 729
FspBI CTAG 2 cut(s) 93, 584
GluI GCNGC 2 cut(s) 51, 729
GsaI CCCAGC 2 cut(s) 504, 653
GsuI CTGGAG 1 cut(s) 872
HgaI GACGC 1 cut(s) 934
Hin1II CATG 7 cut(s) 218, 318, 389, 541, 736, 745, 779
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 3 cut(s) 59, 476, 689
HphI GGTGA 5 cut(s) 3, 125, 181, 284, 358
Hpy166II GTNNAC 3 cut(s) 433, 581, 800
Hpy188I TCNGA 5 cut(s) 33, 157, 418, 970, 1030
Hpy188III TCNNGA 5 cut(s) 93, 172, 386, 458, 473
Hpy8I GTNNAC 3 cut(s) 433, 581, 800
HpyAV CCTTC 5 cut(s) 28, 90, 263, 619, 718
HpyCH4III ACNGT 4 cut(s) 511, 634, 763, 829
HpyCH4V TGCA 4 cut(s) 163, 309, 716, 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 553, 776, 844, 934
HpyF3I CTNAG 3 cut(s) 30, 654, 1074
Hsp92II CATG 7 cut(s) 218, 318, 389, 541, 736, 745, 779
KpnI GGTACC 1 cut(s) 741
Kzo9I GATC 4 cut(s) 186, 302, 504, 1050
LmnI GCTCC 4 cut(s) 453, 490, 790, 1012
Lsp1109I GCAGC 2 cut(s) 37, 740
LweI GCATC 1 cut(s) 924
MaeI CTAG 2 cut(s) 93, 584
MaeIII GTNAC 5 cut(s) 118, 232, 253, 628, 823
MalI GATC 4 cut(s) 188, 304, 506, 1052
MboI GATC 4 cut(s) 186, 302, 504, 1050
MboII GAAGA 3 cut(s) 29, 32, 54
MflI RGATCY 1 cut(s) 302
MhlI GDGCHC 2 cut(s) 450, 487
MluCI AATT 5 cut(s) 260, 603, 753, 792, 981
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 9 cut(s) 39, 143, 480, 652, 658, 791, 876, 905, 976
MseI TTAA 4 cut(s) 672, 791, 984, 999
MslI CAYNNNNRTG 3 cut(s) 384, 493, 1007
MspR9I CCNGG 2 cut(s) 210, 549
MvaI CCWGG 2 cut(s) 210, 549
MwoI GCNNNNNNNGC 4 cut(s) 553, 776, 844, 934
NcoI CCATGG 2 cut(s) 732, 741
NdeII GATC 4 cut(s) 186, 302, 504, 1050
NlaIII CATG 7 cut(s) 218, 318, 389, 541, 736, 745, 779
NlaIV GGNNCC 2 cut(s) 304, 739
NmuCI GTSAC 2 cut(s) 232, 253
NspI RCATGY 1 cut(s) 318
PagI TCATGA 1 cut(s) 385
PciI ACATGT 1 cut(s) 314
PfeI GAWTC 2 cut(s) 476, 689
PflFI GACNNNGTC 1 cut(s) 585
PflMI CCANNNNNTGG 1 cut(s) 794
PkrI GCNGC 2 cut(s) 52, 730
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
PscI ACATGT 1 cut(s) 314
PshBI ATTAAT 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 208, 547
PspFI CCCAGC 2 cut(s) 500, 649
PspGI CCWGG 2 cut(s) 208, 547
PspN4I GGNNCC 2 cut(s) 304, 739
PsrI GAACNNNNNNTAC 2 cut(s) 756, 788
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 2 cut(s) 548, 767
PsuI RGATCY 1 cut(s) 302
PsyI GACNNNGTC 1 cut(s) 585
RsaI GTAC 4 cut(s) 207, 530, 643, 739
RsaNI GTAC 4 cut(s) 206, 529, 642, 738
RseI CAYNNNNRTG 3 cut(s) 384, 493, 1007
SaqAI TTAA 4 cut(s) 672, 791, 984, 999
SatI GCNGC 2 cut(s) 51, 729
Sau3AI GATC 4 cut(s) 186, 302, 504, 1050
ScaI AGTACT 2 cut(s) 530, 643
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 2 cut(s) 210, 549
SduI GDGCHC 2 cut(s) 450, 487
SfaNI GCATC 1 cut(s) 924
SfcI CTRYAG 1 cut(s) 768
SmiMI CAYNNNNRTG 3 cut(s) 384, 493, 1007
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 5 cut(s) 260, 603, 753, 792, 981
SsiI CCGC 1 cut(s) 451
SspI AATATT 1 cut(s) 961
SspMI CTAG 2 cut(s) 93, 584
StyD4I CCNGG 2 cut(s) 208, 547
StyI CCWWGG 2 cut(s) 732, 741
TaaI ACNGT 4 cut(s) 511, 634, 763, 829
TaqI TCGA 2 cut(s) 975, 1053
TasI AATT 5 cut(s) 260, 603, 753, 792, 981
TatI WGTACW 2 cut(s) 528, 641
TfiI GAWTC 2 cut(s) 476, 689
Tru1I TTAA 4 cut(s) 672, 791, 984, 999
Tru9I TTAA 4 cut(s) 672, 791, 984, 999
TscAI CASTG 2 cut(s) 514, 855
TseFI GTSAC 2 cut(s) 232, 253
TseI GCWGC 2 cut(s) 50, 728
Tsp45I GTSAC 2 cut(s) 232, 253
TspDTI ATGAA 4 cut(s) 74, 231, 539, 764
TspRI CASTG 2 cut(s) 514, 855
Tth111I GACNNNGTC 1 cut(s) 585
Van91I CCANNNNNTGG 1 cut(s) 794
VspI ATTAAT 1 cut(s) 791
XapI RAATTY 1 cut(s) 260
XbaI TCTAGA 1 cut(s) 92
XceI RCATGY 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmiI GTMKAC 1 cut(s) 799
XspI CTAG 2 cut(s) 93, 584
ZrmI AGTACT 2 cut(s) 530, 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.