FvH4_1g29440

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004297
Physical Location & Seq
Forward (+)
22535399 .. 22536297
899 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g29440.t1

Sequence Viewer

Length: 588 bp
ATGGGGATATACGAGGTGGTGATGGAGGAGATAACGGCATACACAGGGCTATCACCGGTGGCCTTTTTCACAATCGCTGGGATGATGGTCGTCGTTTACAGATACGTTTCCGGAATGTTTGTGGCTGCCGAAGATTACAACAAGCCTCCGGTGGTGAACTCGGAGTTGGTGAACAAGTATTTCAATCCCACCACCACGACACAAAGCTCAGTCCAAGTCGTCGGGGATATGACGGAGCAAGAGCTTAGAGCATATAATGGGTCTGACCCCAATAAGCCCCTTCTGATGGCCATCAAAGCTCAGATCTACGACGTCTCCTCCTCCAGGAACTTCTACGGTCCAGGTGGGCCGTACGCAATGTTTGCTGGAAGAGATGCTAGCAGAGCCTTGGCTCTTCTCTCTTTCAAACCCCATGACATAAACGGAAACCTCGAGGGCCTCGGTCCCGACGAGCTTCAGATTTTGCAGGACTGGGAGGATAAATTCGTTGAGAAATATGTCAAGGTTGGGCACCTGCTTAACCTTGATTCTCATCCTCTCTCACAACCAACACAAGACACTCAGCAAGCTTCCTATTCTAAACCCTAA

Protein Analysis

196

Amino Acids

21.6

Weight (kDa)

4.79

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 78 - 165 3.5e-10 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 522
AatII GACGTC 1 cut(s) 315
AbsI CCTCGAGG 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 110
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 482
AcuI CTGAAG 1 cut(s) 440
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 2 cut(s) 286, 324
AgeI ACCGGT 1 cut(s) 55
AgsI TTSAA 2 cut(s) 184, 406
AjnI CCWGG 2 cut(s) 323, 340
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 5 cut(s) 207, 244, 299, 454, 569
AluI AGCT 5 cut(s) 207, 244, 299, 454, 569
Alw26I GTCTC 1 cut(s) 319
Ama87I CYCGRG 1 cut(s) 431
Aor13HI TCCGGA 1 cut(s) 110
AoxI GGCC 4 cut(s) 60, 288, 347, 436
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 1 cut(s) 482
AsiGI ACCGGT 1 cut(s) 55
AspS9I GGNCC 4 cut(s) 338, 347, 436, 443
AsuHPI GGTGA 4 cut(s) 31, 45, 166, 181
AsuNHI GCTAGC 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 2 cut(s) 338, 443
BaeGI GKGCMC 1 cut(s) 513
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 112
BccI CCATC 4 cut(s) 16, 79, 280, 299
BceAI ACGGC 2 cut(s) 51, 334
BciT130I CCWGG 2 cut(s) 325, 342
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 1 cut(s) 378
BfuAI ACCTGC 1 cut(s) 522
BglII AGATCT 1 cut(s) 303
BisI GCNGC 1 cut(s) 126
BlsI GCNGC 1 cut(s) 127
Bme1390I CCNGG 2 cut(s) 325, 342
Bme18I GGWCC 2 cut(s) 338, 443
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 4 cut(s) 338, 347, 436, 443
BmiI GGNNCC 2 cut(s) 445, 512
BmrFI CCNGG 2 cut(s) 325, 342
BmrI ACTGGG 1 cut(s) 481
BmsI GCATC 1 cut(s) 364
BmtI GCTAGC 1 cut(s) 381
BmuI ACTGGG 1 cut(s) 481
BpmI CTGGAG 1 cut(s) 307
BsaBI GATNNNNATC 2 cut(s) 290, 531
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 387, 439
BsaWI WCCGGW 3 cut(s) 55, 110, 148
BsaXI ACNNNNNCTCC 4 cut(s) 299, 302, 329, 332
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 324
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 363
Bse8I GATNNNNATC 2 cut(s) 290, 531
BseAI TCCGGA 1 cut(s) 110
BseBI CCWGG 2 cut(s) 325, 342
BseDI CCNNGG 2 cut(s) 387, 439
BseGI GGATG 2 cut(s) 87, 532
BseJI GATNNNNATC 2 cut(s) 290, 531
BseLI CCNNNNNNNGG 2 cut(s) 286, 324
BseMI GCAATG 1 cut(s) 363
BseMII CTCAG 3 cut(s) 222, 314, 575
BseNI ACTGG 1 cut(s) 476
BseRI GAGGAG 3 cut(s) 41, 307, 310
BseSI GKGCMC 1 cut(s) 513
BseXI GCAGC 1 cut(s) 112
BseYI CCCAGC 1 cut(s) 77
BshFI GGCC 4 cut(s) 62, 290, 349, 438
BshNI GGYRCC 1 cut(s) 510
BshTI ACCGGT 1 cut(s) 55
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 3 cut(s) 56, 111, 149
BsiWI CGTACG 1 cut(s) 351
BslFI GGGAC 1 cut(s) 429
BslI CCNNNNNNNGG 2 cut(s) 286, 324
BsmAI GTCTC 1 cut(s) 319
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 429
BsnI GGCC 4 cut(s) 62, 290, 349, 438
BsoBI CYCGRG 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 513
Bsp13I TCCGGA 1 cut(s) 110
Bsp143I GATC 1 cut(s) 303
BspANI GGCC 4 cut(s) 62, 290, 349, 438
BspCNI CTCAG 3 cut(s) 221, 313, 574
BspEI TCCGGA 1 cut(s) 110
BspLI GGNNCC 2 cut(s) 445, 512
BspMI ACCTGC 1 cut(s) 522
BspOI GCTAGC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 399
BspT107I GGYRCC 1 cut(s) 510
BsrDI GCAATG 1 cut(s) 363
BsrFI RCCGGY 1 cut(s) 55
BsrI ACTGG 1 cut(s) 476
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 387, 439
BssMI GATC 1 cut(s) 303
BssNI GRCGYC 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 2 cut(s) 325, 342
Bst4CI ACNGT 1 cut(s) 338
Bst6I CTCTTC 2 cut(s) 364, 399
BstACI GRCGYC 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 362
BstC8I GCNNGC 2 cut(s) 379, 567
BstDEI CTNAG 4 cut(s) 208, 245, 300, 561
BstENI CCTNNNNNAGG 1 cut(s) 322
BstF5I GGATG 2 cut(s) 87, 532
BstKTI GATC 1 cut(s) 306
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 1 cut(s) 303
BstMWI GCNNNNNNNGC 3 cut(s) 296, 362, 383
BstNI CCWGG 2 cut(s) 325, 342
BstSCI CCNGG 2 cut(s) 323, 340
BstSLI GKGCMC 1 cut(s) 513
BstV1I GCAGC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
BsuRI GGCC 4 cut(s) 62, 290, 349, 438
BtsCI GGATG 2 cut(s) 87, 532
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 2 cut(s) 379, 567
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 4 cut(s) 338, 347, 436, 443
Csp6I GTAC 1 cut(s) 352
CspAI ACCGGT 1 cut(s) 55
CviAII CATG 1 cut(s) 413
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 4 cut(s) 208, 245, 300, 561
DpnI GATC 1 cut(s) 305
DpnII GATC 1 cut(s) 303
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 364, 399
EarI CTCTTC 2 cut(s) 364, 399
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 338, 443
Eco57I CTGAAG 1 cut(s) 440
Eco88I CYCGRG 1 cut(s) 431
EcoNI CCTNNNNNAGG 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 436
EcoRII CCWGG 2 cut(s) 323, 340
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 1 cut(s) 416
FaiI YATR 8 cut(s) 10, 40, 230, 253, 255, 414, 419, 498
FaqI GGGAC 1 cut(s) 429
FatI CATG 1 cut(s) 412
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 2 cut(s) 94, 519
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 1 cut(s) 378
GluI GCNGC 1 cut(s) 126
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 307
HaeIII GGCC 4 cut(s) 62, 290, 349, 438
HapII CCGG 3 cut(s) 56, 111, 149
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 1 cut(s) 416
HindIII AAGCTT 1 cut(s) 567
HinfI GANTC 1 cut(s) 527
HpaII CCGG 3 cut(s) 56, 111, 149
HphI GGTGA 4 cut(s) 31, 45, 166, 181
Hpy166II GTNNAC 3 cut(s) 97, 157, 172
Hpy188I TCNGA 5 cut(s) 163, 265, 285, 303, 459
Hpy188III TCNNGA 2 cut(s) 111, 446
Hpy8I GTNNAC 3 cut(s) 97, 157, 172
Hpy99I CGWCG 4 cut(s) 95, 224, 314, 452
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4IV ACGT 2 cut(s) 105, 312
HpyCH4V TGCA 1 cut(s) 466
HpyF10VI GCNNNNNNNGC 3 cut(s) 296, 362, 383
HpyF3I CTNAG 4 cut(s) 208, 245, 300, 561
HpySE526I ACGT 2 cut(s) 105, 312
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 1 cut(s) 416
Kpn2I TCCGGA 1 cut(s) 110
Kzo9I GATC 1 cut(s) 303
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 378
MaeII ACGT 2 cut(s) 105, 312
MalI GATC 1 cut(s) 305
MboI GATC 1 cut(s) 303
MboII GAAGA 3 cut(s) 143, 381, 386
MflI RGATCY 1 cut(s) 303
MhlI GDGCHC 1 cut(s) 513
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 1 cut(s) 482
MluNI TGGCCA 1 cut(s) 290
Mox20I TGGCCA 1 cut(s) 290
MroI TCCGGA 1 cut(s) 110
MscI TGGCCA 1 cut(s) 290
MseI TTAA 1 cut(s) 519
Msp20I TGGCCA 1 cut(s) 290
MspI CCGG 3 cut(s) 56, 111, 149
MspR9I CCNGG 2 cut(s) 325, 342
MvaI CCWGG 2 cut(s) 325, 342
MwoI GCNNNNNNNGC 3 cut(s) 296, 362, 383
NdeII GATC 1 cut(s) 303
NheI GCTAGC 1 cut(s) 377
NlaIII CATG 1 cut(s) 416
NlaIV GGNNCC 2 cut(s) 445, 512
PaeR7I CTCGAG 1 cut(s) 431
PaqCI CACCTGC 1 cut(s) 522
PciSI GCTCTTC 1 cut(s) 399
PcsI WCGNNNNNNNCGW 2 cut(s) 429, 447
PfeI GAWTC 1 cut(s) 527
Pfl23II CGTACG 1 cut(s) 351
PfoI TCCNGGA 1 cut(s) 323
PinAI ACCGGT 1 cut(s) 55
PkrI GCNGC 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 323, 340
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 323, 340
PspLI CGTACG 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 445, 512
PspPI GGNCC 4 cut(s) 338, 347, 436, 443
PspXI VCTCGAGB 1 cut(s) 431
PsuI RGATCY 1 cut(s) 303
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 1 cut(s) 303
Sau96I GGNCC 4 cut(s) 338, 347, 436, 443
ScrFI CCNGG 2 cut(s) 325, 342
SduI GDGCHC 1 cut(s) 513
SfaNI GCATC 1 cut(s) 364
Sfr274I CTCGAG 1 cut(s) 431
SgrAI CRCCGGYG 1 cut(s) 55
SinI GGWCC 2 cut(s) 338, 443
SlaI CTCGAG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 1 cut(s) 482
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 2 cut(s) 323, 340
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 338
TaiI ACGT 2 cut(s) 108, 315
TaqI TCGA 1 cut(s) 432
TaqII GACCGA 1 cut(s) 431
TasI AATT 1 cut(s) 482
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TseI GCWGC 1 cut(s) 125
TspGWI ACGGA 2 cut(s) 248, 438
VpaK11BI GGWCC 2 cut(s) 338, 443
XagI CCTNNNNNAGG 1 cut(s) 322
XapI RAATTY 1 cut(s) 482
XhoI CTCGAG 1 cut(s) 431
XspI CTAG 1 cut(s) 378
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.