MD12G1015300.v1.1

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004297
Physical Location & Seq
Forward (+)
1560491 .. 1562518
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1015300.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGAAGGAATATACGGAGCAGTGATGGAGGAGATATGGCGGTACACGGGGCTGTCACCGACGGCCTTCTTCACAATCGCCGGGATGATGGTCGTGGTTTACAGAATAGTCACTGGCATGTTTGTGGATCCCGAAGACTTCAACCAGCCTCCGGTGGCGGCCACCATCAACACCGTCGATCCTATTTCCAAATCCAATTTTGTGAATAAATACTTCAATTCCACGGGGGCGGCGAGAGGCTCGTCGGTACAAATGGGAGACATGACGGAGGAGCAGCTGAGAGAGTACGGCGGGTCTGACCCCAACAAGCCGCTGCTTATTGCCATCAGAGCTCAGATCTACGACGTCTCTTCCTCCAGGAACTTCTACGGACCAGGTGGACCGTACGCCATGTTTGCTGGAAGGGAAGTTAGCAGAGCGCTGGCTCTTCTGTCTTTTAAACCCCAAGACATAAACGGCAACCTCGAAGGTCTCGGGCCTGATGAACTTGGCATATTACAGGACTGGGAAGAGAAGTTCATCGAAAAATATGACAAGGTCGGGCGGCTTGTAACAGAGCTGAGGATGAATTCTGATGAGTAA

Protein Analysis

194

Amino Acids

21.42

Weight (kDa)

4.62

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 89 - 182 5.4e-08 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 348
AciI CCGC 6 cut(s) 40, 158, 230, 291, 311, 544
AclWI GGATC 3 cut(s) 122, 135, 173
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 568
AcyI GRCGYC 1 cut(s) 345
AfaI GTAC 4 cut(s) 44, 249, 287, 386
AfeI AGCGCT 1 cut(s) 420
AfiI CCNNNNNNNGG 1 cut(s) 151
AgsI TTSAA 2 cut(s) 142, 217
AjnI CCWGG 2 cut(s) 356, 373
AloI GAACNNNNNNTCC 2 cut(s) 500, 532
AluBI AGCT 3 cut(s) 277, 332, 559
AluI AGCT 3 cut(s) 277, 332, 559
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 3 cut(s) 252, 352, 476
AlwI GGATC 3 cut(s) 122, 135, 173
Ama87I CYCGRG 1 cut(s) 473
Aor51HI AGCGCT 1 cut(s) 420
AoxI GGCC 3 cut(s) 63, 159, 476
ApeKI GCWGC 2 cut(s) 274, 313
ApoI RAATTY 1 cut(s) 568
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 3 cut(s) 371, 380, 476
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 48
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 2 cut(s) 371, 380
BamHI GGATCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 334
BbvCI CCTCAGC 1 cut(s) 560
BbvI GCAGC 2 cut(s) 286, 300
BccI CCATC 4 cut(s) 19, 82, 173, 332
BceAI ACGGC 3 cut(s) 78, 304, 472
BciT130I CCWGG 2 cut(s) 358, 375
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 3 cut(s) 252, 352, 476
BfoI RGCGCY 1 cut(s) 422
BglII AGATCT 1 cut(s) 336
BisI GCNGC 6 cut(s) 159, 231, 275, 311, 314, 545
BlsI GCNGC 6 cut(s) 160, 232, 276, 312, 315, 546
Bme1390I CCNGG 3 cut(s) 82, 358, 375
Bme18I GGWCC 2 cut(s) 371, 380
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 3 cut(s) 371, 380, 476
BmiI GGNNCC 1 cut(s) 129
BmrFI CCNGG 3 cut(s) 82, 358, 375
BmrI ACTGGG 1 cut(s) 514
BmuI ACTGGG 1 cut(s) 514
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 560
BpuMI CCSGG 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 345
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 151
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse1I ACTGG 2 cut(s) 118, 509
BseBI CCWGG 2 cut(s) 358, 375
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 2 cut(s) 90, 570
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMII CTCAG 3 cut(s) 269, 347, 551
BseNI ACTGG 2 cut(s) 118, 509
BseRI GAGGAG 2 cut(s) 44, 284
BseXI GCAGC 2 cut(s) 286, 300
BshFI GGCC 3 cut(s) 65, 161, 478
BsiHKAI GWGCWC 1 cut(s) 334
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 2 cut(s) 81, 152
BsiWI CGTACG 1 cut(s) 384
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 3 cut(s) 252, 352, 476
BsmBI CGTCTC 1 cut(s) 352
BsnI GGCC 3 cut(s) 65, 161, 478
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 3 cut(s) 127, 178, 336
BspACI CCGC 6 cut(s) 40, 158, 230, 291, 311, 544
BspANI GGCC 3 cut(s) 65, 161, 478
BspCNI CTCAG 3 cut(s) 270, 346, 552
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 3 cut(s) 122, 135, 173
BspQI GCTCTTC 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 476
BsrI ACTGG 2 cut(s) 118, 509
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 3 cut(s) 127, 178, 336
BssNI GRCGYC 1 cut(s) 345
Bst2UI CCWGG 2 cut(s) 358, 375
Bst4CI ACNGT 2 cut(s) 175, 384
Bst6I CTCTTC 3 cut(s) 355, 432, 504
BstACI GRCGYC 1 cut(s) 345
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 3 cut(s) 278, 333, 560
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 2 cut(s) 90, 570
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 3 cut(s) 130, 181, 339
BstMAI GTCTC 3 cut(s) 252, 352, 476
BstMBI GATC 3 cut(s) 127, 178, 336
BstMWI GCNNNNNNNGC 2 cut(s) 329, 395
BstNI CCWGG 2 cut(s) 358, 375
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 3 cut(s) 80, 356, 373
BstV1I GCAGC 2 cut(s) 286, 300
BstV2I GAAGAC 1 cut(s) 141
BstX2I RGATCY 2 cut(s) 127, 336
BstYI RGATCY 2 cut(s) 127, 336
BsuRI GGCC 3 cut(s) 65, 161, 478
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 2 cut(s) 90, 570
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 27, 111
Cac8I GCNNGC 1 cut(s) 423
CfoI GCGC 1 cut(s) 421
Cfr13I GGNCC 3 cut(s) 371, 380, 476
CsiI ACCWGGT 1 cut(s) 373
Csp6I GTAC 4 cut(s) 43, 248, 286, 385
CviAII CATG 3 cut(s) 118, 262, 391
CviQI GTAC 4 cut(s) 43, 248, 286, 385
DdeI CTNAG 3 cut(s) 278, 333, 560
DpnI GATC 3 cut(s) 129, 180, 338
DpnII GATC 3 cut(s) 127, 178, 336
DraI TTTAAA 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 3 cut(s) 355, 432, 504
EarI CTCTTC 3 cut(s) 355, 432, 504
Ecl136II GAGCTC 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 371, 380
Eco47III AGCGCT 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 473
EcoICRI GAGCTC 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 568
EcoRII CCWGG 2 cut(s) 356, 373
EcoT38I GRGCYC 1 cut(s) 334
Esp3I CGTCTC 1 cut(s) 352
FaeI CATG 3 cut(s) 121, 265, 394
FaiI YATR 8 cut(s) 13, 37, 119, 263, 392, 452, 494, 531
FatI CATG 3 cut(s) 117, 261, 390
FauI CCCGC 1 cut(s) 284
Fnu4HI GCNGC 6 cut(s) 159, 231, 275, 311, 314, 545
FokI GGATG 2 cut(s) 97, 577
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 6 cut(s) 159, 231, 275, 311, 314, 545
GlaI GCGC 1 cut(s) 420
GluI GCNGC 6 cut(s) 159, 231, 275, 311, 314, 545
GsuI CTGGAG 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 3 cut(s) 65, 161, 478
HapII CCGG 2 cut(s) 81, 152
HhaI GCGC 1 cut(s) 421
Hin1I GRCGYC 1 cut(s) 345
Hin1II CATG 3 cut(s) 121, 265, 394
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HpaII CCGG 2 cut(s) 81, 152
HphI GGTGA 1 cut(s) 48
Hpy166II GTNNAC 3 cut(s) 45, 100, 380
Hpy188I TCNGA 4 cut(s) 298, 329, 336, 574
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 3 cut(s) 45, 100, 380
Hpy99I CGWCG 4 cut(s) 64, 179, 247, 347
HpyAV CCTTC 3 cut(s) 76, 396, 461
HpyCH4III ACNGT 2 cut(s) 175, 384
HpyCH4IV ACGT 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 395
HpyF3I CTNAG 3 cut(s) 278, 333, 560
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 345
Hsp92II CATG 3 cut(s) 121, 265, 394
HspAI GCGC 1 cut(s) 419
Kzo9I GATC 3 cut(s) 127, 178, 336
LguI GCTCTTC 1 cut(s) 432
LmnI GCTCC 2 cut(s) 17, 271
Lsp1109I GCAGC 2 cut(s) 286, 300
MabI ACCWGGT 1 cut(s) 373
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 3 cut(s) 54, 109, 550
MalI GATC 3 cut(s) 129, 180, 338
MboI GATC 3 cut(s) 127, 178, 336
MboII GAAGA 5 cut(s) 61, 146, 342, 419, 521
MflI RGATCY 2 cut(s) 127, 336
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 3 cut(s) 196, 217, 568
MnlI CCTC 7 cut(s) 22, 159, 230, 262, 364, 473, 555
MseI TTAA 1 cut(s) 438
MslI CAYNNNNRTG 2 cut(s) 116, 122
MspA1I CMGCKG 2 cut(s) 277, 313
MspI CCGG 2 cut(s) 81, 152
MspR9I CCNGG 3 cut(s) 82, 358, 375
MvaI CCWGG 2 cut(s) 358, 375
MwoI GCNNNNNNNGC 2 cut(s) 329, 395
NciI CCSGG 1 cut(s) 82
NdeII GATC 3 cut(s) 127, 178, 336
NlaIII CATG 3 cut(s) 121, 265, 394
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 2 cut(s) 54, 109
NspI RCATGY 1 cut(s) 121
PciSI GCTCTTC 1 cut(s) 432
PcsI WCGNNNNNNNCGW 2 cut(s) 230, 462
Pfl23II CGTACG 1 cut(s) 384
PflFI GACNNNGTC 1 cut(s) 536
PfoI TCCNGGA 1 cut(s) 356
PkrI GCNGC 6 cut(s) 160, 232, 276, 312, 315, 546
Psp124BI GAGCTC 1 cut(s) 334
Psp6I CCWGG 2 cut(s) 356, 373
PspGI CCWGG 2 cut(s) 356, 373
PspLI CGTACG 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 3 cut(s) 371, 380, 476
PsuI RGATCY 2 cut(s) 127, 336
PsyI GACNNNGTC 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 4 cut(s) 44, 249, 287, 386
RsaNI GTAC 4 cut(s) 43, 248, 286, 385
RseI CAYNNNNRTG 2 cut(s) 116, 122
SacI GAGCTC 1 cut(s) 334
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 6 cut(s) 159, 231, 275, 311, 314, 545
Sau3AI GATC 3 cut(s) 127, 178, 336
Sau96I GGNCC 3 cut(s) 371, 380, 476
ScrFI CCNGG 3 cut(s) 82, 358, 375
SduI GDGCHC 1 cut(s) 334
SetI ASST 8 cut(s) 279, 334, 348, 379, 465, 472, 540, 561
SexAI ACCWGGT 1 cut(s) 373
SinI GGWCC 2 cut(s) 371, 380
SmiMI CAYNNNNRTG 2 cut(s) 116, 122
Sse9I AATT 3 cut(s) 196, 217, 568
SsiI CCGC 6 cut(s) 40, 158, 230, 291, 311, 544
SstI GAGCTC 1 cut(s) 334
StyD4I CCNGG 3 cut(s) 80, 356, 373
TaaI ACNGT 2 cut(s) 175, 384
TaiI ACGT 1 cut(s) 348
TaqI TCGA 3 cut(s) 177, 465, 522
TasI AATT 3 cut(s) 196, 217, 568
TauI GCSGC 4 cut(s) 161, 233, 313, 547
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TscAI CASTG 2 cut(s) 27, 118
TseFI GTSAC 2 cut(s) 54, 109
TseI GCWGC 2 cut(s) 274, 313
Tsp45I GTSAC 2 cut(s) 54, 109
TspDTI ATGAA 3 cut(s) 498, 508, 581
TspGWI ACGGA 3 cut(s) 30, 281, 384
TspRI CASTG 2 cut(s) 27, 118
Tth111I GACNNNGTC 1 cut(s) 536
VpaK11BI GGWCC 2 cut(s) 371, 380
XapI RAATTY 1 cut(s) 568
XceI RCATGY 1 cut(s) 121
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.