RchiOBHm_Chr3g0495341

Belongs to the cytochrome b5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004297
Physical Location & Seq
Reverse (-)
44819911 .. 44821332
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45779

Sequence Viewer

Length: 576 bp
ATGGGGATATACGAGGTGGTGATGGAGGAGATAACGGTGTACACGGGGCTATCACCGGCGGCGTTTTTCACAATCGCTGGGATGATGGTCGTCGTTTACAGATATGTTACCGGAATGTTTGTGGCTCCCGAAGATTACAACAAGCCTCCGGTGGTGAGTAGTGCTAATTCGGAATTGGCGAACAAGTATTTCAATCCCACGACCGCCACGACACCAAGCTCAATCCAAGTCGGGGATATGACGGAGCAAGAGCTTAGAGGATATGATGGGTCTGACCCCAATAAGCCCCTTCTCATGGCCATCAAAGCTCAGATCTACGACGTCTCCTCCTCCAGGAACTTCTATGGTCCAGGTGGACCGTATGCAATGTTTGCTGGAAGGGATGCTAGCAGAGCCTTGGCTCTTCTGTCTTTCAAACCCCAAGACATACACGGAAACATCGATGGCCTCGGTCCCGACGAGCTTCAGATTTTACAGGACTGGGAAGATAAATTCGTTGAAAAATATCTCACGGTTGGACGGCTGCTTAACCGTGATCCTGTGCCACAACCAACACAAGAGACTAAGCAAACTTGA

Protein Analysis

191

Amino Acids

21.11

Weight (kDa)

4.62

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 81 - 168 2.5e-09 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 324
AciI CCGC 2 cut(s) 59, 204
AclWI GGATC 1 cut(s) 530
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 3 cut(s) 232, 295, 333
AgsI TTSAA 3 cut(s) 193, 415, 500
AjnI CCWGG 2 cut(s) 332, 349
AluBI AGCT 4 cut(s) 219, 253, 308, 463
AluI AGCT 4 cut(s) 219, 253, 308, 463
Alw26I GTCTC 2 cut(s) 328, 554
AlwI GGATC 1 cut(s) 530
AoxI GGCC 2 cut(s) 297, 445
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 1 cut(s) 491
AspS9I GGNCC 3 cut(s) 347, 356, 452
AsuHPI GGTGA 3 cut(s) 31, 45, 166
AsuNHI GCTAGC 1 cut(s) 386
AvaII GGWCC 3 cut(s) 347, 356, 452
BalI TGGCCA 1 cut(s) 299
BbvI GCAGC 1 cut(s) 510
BccI CCATC 5 cut(s) 16, 79, 260, 308, 437
BceAI ACGGC 1 cut(s) 536
BciT130I CCWGG 2 cut(s) 334, 351
BcoDI GTCTC 2 cut(s) 328, 554
BfaI CTAG 1 cut(s) 387
BglII AGATCT 1 cut(s) 312
BisI GCNGC 2 cut(s) 60, 524
BlsI GCNGC 2 cut(s) 61, 525
Bme1390I CCNGG 2 cut(s) 334, 351
Bme18I GGWCC 3 cut(s) 347, 356, 452
BmgT120I GGNCC 3 cut(s) 347, 356, 452
BmiI GGNNCC 2 cut(s) 126, 454
BmrFI CCNGG 2 cut(s) 334, 351
BmrI ACTGGG 1 cut(s) 490
BmsI GCATC 1 cut(s) 373
BmtI GCTAGC 1 cut(s) 390
BmuI ACTGGG 1 cut(s) 490
BpmI CTGGAG 1 cut(s) 316
Bsa29I ATCGAT 1 cut(s) 441
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 396, 448
BsaWI WCCGGW 2 cut(s) 110, 148
BsaXI ACNNNNNCTCC 4 cut(s) 308, 311, 338, 341
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 295, 333
Bse118I RCCGGY 1 cut(s) 55
Bse1I ACTGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 2 cut(s) 334, 351
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 2 cut(s) 396, 448
BseGI GGATG 2 cut(s) 87, 388
BseLI CCNNNNNNNGG 3 cut(s) 232, 295, 333
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 3 cut(s) 41, 316, 319
BseXI GCAGC 1 cut(s) 510
BseYI CCCAGC 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 299, 447
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 3 cut(s) 56, 111, 149
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 3 cut(s) 232, 295, 333
BsmAI GTCTC 2 cut(s) 328, 554
BsmBI CGTCTC 1 cut(s) 328
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 2 cut(s) 299, 447
Bsp1407I TGTACA 1 cut(s) 39
Bsp143I GATC 2 cut(s) 312, 535
BspACI CCGC 2 cut(s) 59, 204
BspANI GGCC 2 cut(s) 299, 447
BspCNI CTCAG 1 cut(s) 322
BspDI ATCGAT 1 cut(s) 441
BspLI GGNNCC 2 cut(s) 126, 454
BspOI GCTAGC 1 cut(s) 390
BspPI GGATC 1 cut(s) 530
BspQI GCTCTTC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 55
BsrGI TGTACA 1 cut(s) 39
BsrI ACTGG 1 cut(s) 485
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 396, 448
BssMI GATC 2 cut(s) 312, 535
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 334, 351
Bst4CI ACNGT 4 cut(s) 37, 360, 514, 533
Bst6I CTCTTC 1 cut(s) 408
BstACI GRCGYC 1 cut(s) 321
BstAPI GCANNNNNTGC 1 cut(s) 371
BstAUI TGTACA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 3 cut(s) 254, 309, 564
BstENI CCTNNNNNAGG 1 cut(s) 331
BstF5I GGATG 2 cut(s) 87, 388
BstKTI GATC 2 cut(s) 315, 538
BstMAI GTCTC 2 cut(s) 328, 554
BstMBI GATC 2 cut(s) 312, 535
BstMCI CGRYCG 1 cut(s) 204
BstMWI GCNNNNNNNGC 3 cut(s) 305, 371, 392
BstNI CCWGG 2 cut(s) 334, 351
BstSCI CCNGG 2 cut(s) 332, 349
BstV1I GCAGC 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 312
BstYI RGATCY 1 cut(s) 312
Bsu15I ATCGAT 1 cut(s) 441
BsuRI GGCC 2 cut(s) 299, 447
BsuTUI ATCGAT 1 cut(s) 441
BtsCI GGATG 2 cut(s) 87, 388
Cac8I GCNNGC 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 3 cut(s) 347, 356, 452
ClaI ATCGAT 1 cut(s) 441
Csp6I GTAC 1 cut(s) 40
CviAII CATG 1 cut(s) 295
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 3 cut(s) 254, 309, 564
DpnI GATC 2 cut(s) 314, 537
DpnII GATC 2 cut(s) 312, 535
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco130I CCWWGG 1 cut(s) 396
Eco47I GGWCC 3 cut(s) 347, 356, 452
Eco57I CTGAAG 1 cut(s) 449
EcoNI CCTNNNNNAGG 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 332, 349
EcoT14I CCWWGG 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 328
FaeI CATG 1 cut(s) 298
FaiI YATR 8 cut(s) 10, 105, 239, 264, 296, 345, 363, 428
FaqI GGGAC 1 cut(s) 438
FatI CATG 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 60, 524
FokI GGATG 2 cut(s) 94, 395
Fsp4HI GCNGC 2 cut(s) 60, 524
FspBI CTAG 1 cut(s) 387
GluI GCNGC 2 cut(s) 60, 524
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 316
HaeIII GGCC 2 cut(s) 299, 447
HapII CCGG 3 cut(s) 56, 111, 149
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 1 cut(s) 298
HpaII CCGG 3 cut(s) 56, 111, 149
HphI GGTGA 3 cut(s) 31, 45, 166
Hpy166II GTNNAC 4 cut(s) 40, 42, 97, 356
Hpy188I TCNGA 4 cut(s) 172, 274, 312, 468
Hpy188III TCNNGA 2 cut(s) 128, 455
Hpy8I GTNNAC 4 cut(s) 40, 42, 97, 356
Hpy99I CGWCG 3 cut(s) 95, 323, 461
HpyAV CCTTC 2 cut(s) 299, 372
HpyCH4III ACNGT 4 cut(s) 37, 360, 514, 533
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 371, 392
HpyF3I CTNAG 3 cut(s) 254, 309, 564
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 1 cut(s) 298
Kzo9I GATC 2 cut(s) 312, 535
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 2 cut(s) 130, 244
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 1 cut(s) 373
MaeI CTAG 1 cut(s) 387
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 314, 537
MboI GATC 2 cut(s) 312, 535
MboII GAAGA 3 cut(s) 143, 395, 497
MflI RGATCY 1 cut(s) 312
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 3 cut(s) 166, 173, 491
MluNI TGGCCA 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 496
MnlI CCTC 7 cut(s) 7, 19, 156, 251, 337, 340, 458
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
MseI TTAA 1 cut(s) 528
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 3 cut(s) 56, 111, 149
MspR9I CCNGG 2 cut(s) 334, 351
MvaI CCWGG 2 cut(s) 334, 351
MwoI GCNNNNNNNGC 3 cut(s) 305, 371, 392
NdeII GATC 2 cut(s) 312, 535
NheI GCTAGC 1 cut(s) 386
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 2 cut(s) 126, 454
PciSI GCTCTTC 1 cut(s) 408
PcsI WCGNNNNNNNCGW 4 cut(s) 176, 206, 438, 456
PfoI TCCNGGA 1 cut(s) 332
PkrI GCNGC 2 cut(s) 61, 525
Psp6I CCWGG 2 cut(s) 332, 349
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 332, 349
PspN4I GGNNCC 2 cut(s) 126, 454
PspPI GGNCC 3 cut(s) 347, 356, 452
PsuI RGATCY 1 cut(s) 312
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 1 cut(s) 528
SatI GCNGC 2 cut(s) 60, 524
Sau3AI GATC 2 cut(s) 312, 535
Sau96I GGNCC 3 cut(s) 347, 356, 452
ScrFI CCNGG 2 cut(s) 334, 351
SetI ASST 7 cut(s) 18, 221, 255, 310, 324, 355, 465
SfaNI GCATC 1 cut(s) 373
SgrAI CRCCGGYG 1 cut(s) 55
SinI GGWCC 3 cut(s) 347, 356, 452
Sse9I AATT 3 cut(s) 166, 173, 491
SsiI CCGC 2 cut(s) 59, 204
SspMI CTAG 1 cut(s) 387
StyD4I CCNGG 2 cut(s) 332, 349
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 4 cut(s) 37, 360, 514, 533
TaiI ACGT 1 cut(s) 324
TaqI TCGA 1 cut(s) 441
TaqII GACCGA 1 cut(s) 440
TasI AATT 3 cut(s) 166, 173, 491
TatI WGTACW 1 cut(s) 39
TauI GCSGC 1 cut(s) 62
Tru1I TTAA 1 cut(s) 528
Tru9I TTAA 1 cut(s) 528
TseI GCWGC 1 cut(s) 523
TspGWI ACGGA 2 cut(s) 257, 447
VpaK11BI GGWCC 3 cut(s) 347, 356, 452
XagI CCTNNNNNAGG 1 cut(s) 331
XapI RAATTY 1 cut(s) 491
XspI CTAG 1 cut(s) 387
ZraI GACGTC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.