FvH4_2g01660

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006047
Physical Location & Seq
Reverse (-)
1485252 .. 1486234
983 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01660.t1

Sequence Viewer

Length: 579 bp
ATGGAGAAGGCAGTTCTAAAGCTTTTCTTCGTAGCTGTGTTCATGTTCATTCCATTACTAGGGCACAGTAGTAGTAGCACTGAAGAAAACCGAGAACTGGACCGATGGTTTCAGAAGCTCGACTACTCCAAACCGAAGCTTACCCGATTCCACTTCTACTTTCACGACACCGTTTCAGGCCCCGACCAAACATCACTCCCAGTGGTTCCATCACCCAAGAGCATCAAGCCATCCCTCACCTTGTTCGGACAAGTCAATATCTTTGATAACCCCCTAACCAAGGAACCCGAAATCACGTCCGAGCGCCTAGGCGTGGCTCAGGGGCTCTACAGTTTCGCGTCGCAAGAAGAATATAGTGTACTTTTGGCAATGACCATTGTCTTCACCAGCGGCACAAACAACGGTAGCAGTGTCACTATTTTGGGGCGCAATCCAGTGCTTCAACCCCGGCGAGAGTTTCCTATCGTCGGCGGGACTGGCGATTTCCGGTTGGCTCGTGGGTTTGCTACGGCCTCGAATTATGTTGCTAATGCTAGCATGGCTATTGTAGAATATAATGTCACGGTTTTACATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

193

Amino Acids

21.41

Weight (kDa)

6.91

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 48 - 189 5.3e-49 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 338
AciI CCGC 2 cut(s) 390, 471
AcuI CTGAAG 1 cut(s) 102
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 5 cut(s) 59, 97, 280, 313, 467
AgsI TTSAA 1 cut(s) 443
AjiI CACGTC 1 cut(s) 297
AluBI AGCT 4 cut(s) 22, 35, 118, 139
AluI AGCT 4 cut(s) 22, 35, 118, 139
AoxI GGCC 2 cut(s) 178, 510
AspA2I CCTAGG 1 cut(s) 307
AspLEI GCGC 2 cut(s) 306, 429
AspS9I GGNCC 2 cut(s) 100, 179
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 3 cut(s) 204, 229, 376
AsuNHI GCTAGC 1 cut(s) 533
AvaII GGWCC 1 cut(s) 100
AvrII CCTAGG 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 327
BarI GAAGNNNNNNTAC 2 cut(s) 107, 139
BauI CACGAG 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 373
BccI CCATC 3 cut(s) 99, 217, 238
BceAI ACGGC 1 cut(s) 525
BcnI CCSGG 1 cut(s) 448
BfaI CTAG 3 cut(s) 59, 308, 534
BfmI CTRYAG 1 cut(s) 328
BfoI RGCGCY 1 cut(s) 307
BisI GCNGC 1 cut(s) 391
BlnI CCTAGG 1 cut(s) 307
BlsI GCNGC 1 cut(s) 392
Bme1390I CCNGG 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 100
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 2 cut(s) 100, 179
BmiI GGNNCC 3 cut(s) 181, 207, 285
BmrFI CCNGG 1 cut(s) 448
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 1 cut(s) 231
BmtI GCTAGC 1 cut(s) 537
BmuI ACTGGG 1 cut(s) 194
BoxI GACNNNNGTC 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 373
Bpu10I CCTNAGC 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 448
BsaJI CCNNGG 3 cut(s) 279, 307, 446
BsaWI WCCGGW 1 cut(s) 486
Bsc4I CCNNNNNNNGG 5 cut(s) 59, 97, 280, 313, 467
Bse1I ACTGG 4 cut(s) 102, 200, 434, 481
Bse3DI GCAATG 1 cut(s) 375
BseDI CCNNGG 3 cut(s) 279, 307, 446
BseGI GGATG 1 cut(s) 230
BseLI CCNNNNNNNGG 5 cut(s) 59, 97, 280, 313, 467
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 4 cut(s) 102, 200, 434, 481
BseSI GKGCMC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 338
BshFI GGCC 2 cut(s) 180, 512
BsiSI CCGG 2 cut(s) 448, 487
BslFI GGGAC 1 cut(s) 487
BslI CCNNNNNNNGG 5 cut(s) 59, 97, 280, 313, 467
BsmFI GGGAC 1 cut(s) 487
BsnI GGCC 2 cut(s) 180, 512
Bsp1286I GDGCHC 2 cut(s) 66, 327
BspACI CCGC 2 cut(s) 390, 471
BspANI GGCC 2 cut(s) 180, 512
BspCNI CTCAG 1 cut(s) 331
BspFNI CGCG 1 cut(s) 338
BspLI GGNNCC 3 cut(s) 181, 207, 285
BspOI GCTAGC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 4 cut(s) 102, 200, 434, 481
BssECI CCNNGG 3 cut(s) 279, 307, 446
BssSI CACGAG 1 cut(s) 495
BssT1I CCWWGG 2 cut(s) 279, 307
Bst2BI CACGAG 1 cut(s) 495
Bst4CI ACNGT 5 cut(s) 68, 172, 332, 404, 565
BstC8I GCNNGC 1 cut(s) 535
BstDEI CTNAG 1 cut(s) 318
BstENI CCTNNNNNAGG 1 cut(s) 278
BstF5I GGATG 1 cut(s) 230
BstFNI CGCG 1 cut(s) 338
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 2 cut(s) 306, 429
BstMWI GCNNNNNNNGC 2 cut(s) 477, 539
BstPAI GACNNNNGTC 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 446
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 66
BstUI CGCG 1 cut(s) 338
BstV2I GAAGAC 1 cut(s) 373
BsuRI GGCC 2 cut(s) 180, 512
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 1 cut(s) 230
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 4 cut(s) 78, 207, 415, 441
Cac8I GCNNGC 1 cut(s) 535
CfoI GCGC 2 cut(s) 306, 429
Cfr13I GGNCC 2 cut(s) 100, 179
CseI GACGC 1 cut(s) 327
Csp6I GTAC 1 cut(s) 359
CviAII CATG 2 cut(s) 43, 538
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 1 cut(s) 318
Eco130I CCWWGG 2 cut(s) 279, 307
Eco24I GRGCYC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 100
Eco57I CTGAAG 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 179
EcoT14I CCWWGG 2 cut(s) 279, 307
EcoT38I GRGCYC 1 cut(s) 327
ErhI CCWWGG 2 cut(s) 279, 307
FaeI CATG 2 cut(s) 46, 541
FaiI YATR 5 cut(s) 44, 354, 522, 539, 555
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 487
FatI CATG 2 cut(s) 42, 537
FauI CCCGC 1 cut(s) 464
Fnu4HI GCNGC 1 cut(s) 391
FokI GGATG 1 cut(s) 217
FriOI GRGCYC 1 cut(s) 327
Fsp4HI GCNGC 1 cut(s) 391
FspBI CTAG 3 cut(s) 59, 308, 534
GlaI GCGC 2 cut(s) 305, 428
GluI GCNGC 1 cut(s) 391
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 2 cut(s) 180, 512
HapII CCGG 2 cut(s) 448, 487
HgaI GACGC 1 cut(s) 327
HhaI GCGC 2 cut(s) 306, 429
Hin1II CATG 2 cut(s) 46, 541
Hin6I GCGC 2 cut(s) 304, 427
HinP1I GCGC 2 cut(s) 304, 427
HindIII AAGCTT 2 cut(s) 20, 137
HinfI GANTC 1 cut(s) 147
HpaII CCGG 2 cut(s) 448, 487
HphI GGTGA 3 cut(s) 204, 229, 376
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 3 cut(s) 114, 248, 301
Hpy188III TCNNGA 1 cut(s) 164
Hpy8I GTNNAC 1 cut(s) 359
Hpy99I CGWCG 2 cut(s) 343, 470
HpyCH4III ACNGT 5 cut(s) 68, 172, 332, 404, 565
HpyCH4IV ACGT 1 cut(s) 296
HpyF10VI GCNNNNNNNGC 2 cut(s) 477, 539
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 1 cut(s) 296
Hsp92II CATG 2 cut(s) 46, 541
HspAI GCGC 2 cut(s) 304, 427
LpnPI CCDG 9 cut(s) 83, 162, 213, 305, 400, 447, 461, 462, 500
LweI GCATC 1 cut(s) 231
MaeI CTAG 3 cut(s) 59, 308, 534
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 2 cut(s) 412, 559
MboII GAAGA 4 cut(s) 19, 95, 359, 373
MhlI GDGCHC 2 cut(s) 66, 327
MluCI AATT 1 cut(s) 517
MnlI CCTC 2 cut(s) 245, 523
MspA1I CMGCKG 1 cut(s) 390
MspI CCGG 2 cut(s) 448, 487
MspR9I CCNGG 1 cut(s) 448
MvnI CGCG 1 cut(s) 338
MwoI GCNNNNNNNGC 2 cut(s) 477, 539
NciI CCSGG 1 cut(s) 448
NheI GCTAGC 1 cut(s) 533
NlaIII CATG 2 cut(s) 46, 541
NlaIV GGNNCC 3 cut(s) 181, 207, 285
NmuCI GTSAC 2 cut(s) 412, 559
PfeI GAWTC 1 cut(s) 147
PkrI GCNGC 1 cut(s) 392
PshAI GACNNNNGTC 1 cut(s) 377
PspN4I GGNNCC 3 cut(s) 181, 207, 285
PspPI GGNCC 2 cut(s) 100, 179
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
SatI GCNGC 1 cut(s) 391
Sau96I GGNCC 2 cut(s) 100, 179
ScrFI CCNGG 1 cut(s) 448
SduI GDGCHC 2 cut(s) 66, 327
SetI ASST 6 cut(s) 24, 37, 120, 141, 242, 299
SfaNI GCATC 1 cut(s) 231
SfcI CTRYAG 1 cut(s) 328
SinI GGWCC 1 cut(s) 100
Sse9I AATT 1 cut(s) 517
SsiI CCGC 2 cut(s) 390, 471
SspMI CTAG 3 cut(s) 59, 308, 534
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 2 cut(s) 279, 307
TaaI ACNGT 5 cut(s) 68, 172, 332, 404, 565
TaiI ACGT 1 cut(s) 299
TaqI TCGA 2 cut(s) 120, 515
TaqII GACCGA 1 cut(s) 117
TasI AATT 1 cut(s) 517
TatI WGTACW 1 cut(s) 358
TauI GCSGC 1 cut(s) 393
TfiI GAWTC 1 cut(s) 147
TscAI CASTG 4 cut(s) 85, 207, 415, 441
TseFI GTSAC 2 cut(s) 412, 559
Tsp45I GTSAC 2 cut(s) 412, 559
TspDTI ATGAA 2 cut(s) 31, 37
TspRI CASTG 4 cut(s) 85, 207, 415, 441
VpaK11BI GGWCC 1 cut(s) 100
XagI CCTNNNNNAGG 1 cut(s) 278
XmaJI CCTAGG 1 cut(s) 307
XspI CTAG 3 cut(s) 59, 308, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.