RLG00000015349

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006047
Physical Location & Seq
Forward (+)
64890637 .. 64891084
448 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015349

Sequence Viewer

Length: 354 bp
ATGTTTGACAACCCACTAACCAAGGGACCCGAACTCACGTCCGAGTTTGTAGGCAGGGCTCAGGGGCTATACAGTTTCGCGTCCCAAGAAGAGCTCTGTCTACTTATGGCCATGACCATTGTTTTCACCAGCGGCAAACACAACGGTAGCGGTGTCACTATTTTGGGGCGCAACGCAGTGTTTCAATCCCGGCGAGAGTTACCAATTGTCGGCGGAACTGGTGATTTCCGGTTGGCCCGTGGCTTTGCGTCAGTCTCGAGCTATTTTATTAATGCTACTCTTGGGACTCTGCCGAGAGAATTAGTGGCTTTTGCCGGAGATATGCGAGAATTCTATTTGGTTTTGAAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

118

Amino Acids

12.82

Weight (kDa)

8.98

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 1 - 95 6e-35 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 100
AccII CGCG 1 cut(s) 80
AciI CCGC 3 cut(s) 132, 150, 213
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 329
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 2 cut(s) 185, 346
AjiI CACGTC 1 cut(s) 39
AluBI AGCT 2 cut(s) 94, 261
AluI AGCT 2 cut(s) 94, 261
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 259
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 2 cut(s) 108, 234
ApoI RAATTY 1 cut(s) 329
AseI ATTAAT 1 cut(s) 270
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 2 cut(s) 26, 235
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 2 cut(s) 118, 233
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 1 cut(s) 26
BalI TGGCCA 1 cut(s) 110
BanII GRGCYC 2 cut(s) 61, 96
Bbv12I GWGCWC 1 cut(s) 96
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 259
BisI GCNGC 1 cut(s) 133
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 1 cut(s) 190
Bme18I GGWCC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 256
BmgBI CACGTC 1 cut(s) 39
BmgT120I GGNCC 2 cut(s) 26, 235
BmiI GGNNCC 2 cut(s) 27, 28
BmrFI CCNGG 1 cut(s) 190
Bpu10I CCTNAGC 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 190
BsaJI CCNNGG 2 cut(s) 21, 238
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 223
BseDI CCNNGG 2 cut(s) 21, 238
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 80
BshFI GGCC 2 cut(s) 110, 236
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 3 cut(s) 190, 229, 315
BslFI GGGAC 3 cut(s) 39, 67, 298
BslI CCNNNNNNNGG 1 cut(s) 209
BsmAI GTCTC 1 cut(s) 259
BsmFI GGGAC 3 cut(s) 39, 67, 298
BsnI GGCC 2 cut(s) 110, 236
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 61, 96
BspACI CCGC 3 cut(s) 132, 150, 213
BspANI GGCC 2 cut(s) 110, 236
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 2 cut(s) 27, 28
BspQI GCTCTTC 1 cut(s) 84
BsrI ACTGG 1 cut(s) 223
BssECI CCNNGG 2 cut(s) 21, 238
BssT1I CCWWGG 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 74, 146
Bst6I CTCTTC 2 cut(s) 84, 341
BstDEI CTNAG 1 cut(s) 60
BstDSI CCRYGG 1 cut(s) 238
BstFNI CGCG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 171
BstMAI GTCTC 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 188
BstUI CGCG 1 cut(s) 80
BsuRI GGCC 2 cut(s) 110, 236
BtgI CCRYGG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 183
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 2 cut(s) 26, 235
CseI GACGC 2 cut(s) 69, 237
CviAII CATG 1 cut(s) 112
CviJI RGCY 8 cut(s) 59, 67, 94, 110, 236, 243, 261, 308
CviKI_1 RGCY 8 cut(s) 59, 67, 94, 110, 236, 243, 261, 308
DdeI CTNAG 1 cut(s) 60
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 2 cut(s) 84, 341
EarI CTCTTC 2 cut(s) 84, 341
EciI GGCGGA 1 cut(s) 228
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 61, 96
Eco47I GGWCC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 94
Eco88I CYCGRG 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 26
EcoRI GAATTC 1 cut(s) 329
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 2 cut(s) 61, 96
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 1 cut(s) 115
FaiI YATR 4 cut(s) 70, 107, 113, 323
FaqI GGGAC 3 cut(s) 39, 67, 298
FatI CATG 1 cut(s) 111
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 1 cut(s) 133
FriOI GRGCYC 2 cut(s) 61, 96
Fsp4HI GCNGC 1 cut(s) 133
GlaI GCGC 1 cut(s) 170
GluI GCNGC 1 cut(s) 133
HaeIII GGCC 2 cut(s) 110, 236
HapII CCGG 3 cut(s) 190, 229, 315
HgaI GACGC 2 cut(s) 69, 237
HhaI GCGC 1 cut(s) 171
Hin1II CATG 1 cut(s) 115
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 1 cut(s) 286
HpaII CCGG 3 cut(s) 190, 229, 315
HphI GGTGA 2 cut(s) 118, 233
Hpy166II GTNNAC 1 cut(s) 101
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 1 cut(s) 101
HpyCH4III ACNGT 2 cut(s) 74, 146
HpyCH4IV ACGT 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 1 cut(s) 115
HspAI GCGC 1 cut(s) 169
KflI GGGWCCC 1 cut(s) 26
LguI GCTCTTC 1 cut(s) 84
LpnPI CCDG 7 cut(s) 40, 47, 142, 203, 204, 242, 328
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 2 cut(s) 154, 198
MboII GAAGA 1 cut(s) 101
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 2 cut(s) 61, 96
MlsI TGGCCA 1 cut(s) 110
MluCI AATT 3 cut(s) 204, 299, 329
MluNI TGGCCA 1 cut(s) 110
MlyI GAGTC 1 cut(s) 280
Mox20I TGGCCA 1 cut(s) 110
MscI TGGCCA 1 cut(s) 110
MseI TTAA 1 cut(s) 270
Msp20I TGGCCA 1 cut(s) 110
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 3 cut(s) 190, 229, 315
MspR9I CCNGG 1 cut(s) 190
MunI CAATTG 1 cut(s) 204
MvnI CGCG 1 cut(s) 80
NciI CCSGG 1 cut(s) 190
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 2 cut(s) 27, 28
NmeAIII GCCGAG 1 cut(s) 318
NmuCI GTSAC 1 cut(s) 154
PaeR7I CTCGAG 1 cut(s) 256
PciSI GCTCTTC 1 cut(s) 84
PkrI GCNGC 1 cut(s) 134
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
PpuMI RGGWCCY 1 cut(s) 26
PshBI ATTAAT 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 27, 28
PspPI GGNCC 2 cut(s) 26, 235
PspPPI RGGWCCY 1 cut(s) 26
SacI GAGCTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 270
SatI GCNGC 1 cut(s) 133
Sau96I GGNCC 2 cut(s) 26, 235
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 190
SduI GDGCHC 2 cut(s) 61, 96
SetI ASST 3 cut(s) 41, 96, 263
Sfr274I CTCGAG 1 cut(s) 256
SinI GGWCC 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 256
SmoI CTYRAG 1 cut(s) 256
Sse9I AATT 3 cut(s) 204, 299, 329
SsiI CCGC 3 cut(s) 132, 150, 213
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 188
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 74, 146
TaiI ACGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 257
TasI AATT 3 cut(s) 204, 299, 329
TauI GCSGC 1 cut(s) 135
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 1 cut(s) 154
Tsp45I GTSAC 1 cut(s) 154
TspRI CASTG 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 26
VspI ATTAAT 1 cut(s) 270
XapI RAATTY 1 cut(s) 329
XhoI CTCGAG 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.