Rh6AG025100

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0006047
Physical Location & Seq
Reverse (-)
2901932 .. 2911794
9863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG025100.1

Sequence Viewer

Length: 522 bp
ATGGTAGTCCCACCACCCAAGACTGTCAAGCCATCCACCACCTTGTTTGGACAAGTCACCATGTTTGACGACCCGCTAACCAAGGGACCCGAACTCACGTCCAAGCTCTTAGGTGGAGGCCAGGGGCTATACTTGCATCCCAAGAGGGAAATCAGTCTACTTATGGCTGTGACCTTTGTTCTCAGTAGTGGGAAGCACAATGGCAGCAGTTTCACTATCTTGGGGCGCAATGCAGTGGAACATTCCCCGCGAGAGTTTCCGATTGTAGGTGGAACCGGAAATTTCCGATTGGCCCGAGGGTTTGCCATTGCAAAGACTTTTCTTGTTAATTCTAGTGTTGCTATTGTGGAATATAAATGTTGCAATTATGCATTATTGAATGCAAGAATGAAAATGGCCGGTGTGCGTCTCTTAAGAAGATCGAGAAAGGTGGGACGTCCACTTGGAGCTCGTAACAAACCAAAAGTGGAGGATCTAAATTCCTTGAAGTCGGAGTCTGCATCATCTATGGCGAGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

173

Amino Acids

18.73

Weight (kDa)

10.57

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 2 - 120 1.1e-37 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 439
AccI GTMKAC 1 cut(s) 157
AccII CGCG 1 cut(s) 250
AciI CCGC 2 cut(s) 74, 248
AclWI GGATC 1 cut(s) 480
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 2 cut(s) 280, 478
AcyI GRCGYC 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 266
AflII CTTAAG 1 cut(s) 412
AgsI TTSAA 2 cut(s) 379, 487
AjiI CACGTC 1 cut(s) 99
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 2 cut(s) 106, 449
AluI AGCT 2 cut(s) 106, 449
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 413
AlwI GGATC 1 cut(s) 480
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 3 cut(s) 118, 291, 396
ApeKI GCWGC 1 cut(s) 204
ApoI RAATTY 2 cut(s) 280, 478
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 2 cut(s) 86, 292
AsuHPI GGTGA 1 cut(s) 49
AvaI CYCGRG 1 cut(s) 294
AvaII GGWCC 1 cut(s) 86
BanII GRGCYC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 1 cut(s) 216
BccI CCATC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 413
BfaI CTAG 1 cut(s) 333
BfrI CTTAAG 1 cut(s) 412
BisI GCNGC 1 cut(s) 205
BlsI GCNGC 1 cut(s) 206
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 86
BmeT110I CYCGRG 1 cut(s) 294
BmgBI CACGTC 1 cut(s) 99
BmgT120I GGNCC 2 cut(s) 86, 292
BmiI GGNNCC 3 cut(s) 87, 88, 274
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 2 cut(s) 145, 509
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 3 cut(s) 81, 121, 295
BsaWI WCCGGW 1 cut(s) 275
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse118I RCCGGY 1 cut(s) 398
Bse3DI GCAATG 2 cut(s) 235, 306
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 3 cut(s) 81, 121, 295
BseGI GGATG 2 cut(s) 32, 136
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMI GCAATG 2 cut(s) 235, 306
BseMII CTCAG 1 cut(s) 196
BseXI GCAGC 1 cut(s) 216
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 3 cut(s) 120, 293, 398
BsiHKAI GWGCWC 1 cut(s) 451
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 2 cut(s) 276, 399
BslFI GGGAC 2 cut(s) 99, 447
BslI CCNNNNNNNGG 1 cut(s) 266
BsmAI GTCTC 1 cut(s) 413
BsmBI CGTCTC 1 cut(s) 413
BsmFI GGGAC 2 cut(s) 99, 447
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 3 cut(s) 120, 293, 398
BsoBI CYCGRG 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 451
Bsp143I GATC 2 cut(s) 419, 472
BspACI CCGC 2 cut(s) 74, 248
BspANI GGCC 3 cut(s) 120, 293, 398
BspCNI CTCAG 1 cut(s) 195
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 87, 88, 274
BspPI GGATC 1 cut(s) 480
BspTI CTTAAG 1 cut(s) 412
BsrDI GCAATG 2 cut(s) 235, 306
BsrFI RCCGGY 1 cut(s) 398
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 81, 121, 295
BssMI GATC 2 cut(s) 419, 472
BssNI GRCGYC 1 cut(s) 436
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 25
BstACI GRCGYC 1 cut(s) 436
BstAFI CTTAAG 1 cut(s) 412
BstDEI CTNAG 2 cut(s) 109, 182
BstF5I GGATG 2 cut(s) 32, 136
BstFNI CGCG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 2 cut(s) 422, 475
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 2 cut(s) 419, 472
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 1 cut(s) 122
BstSCI CCNGG 1 cut(s) 120
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BsuRI GGCC 3 cut(s) 120, 293, 398
BtrI CACGTC 1 cut(s) 99
BtsCI GGATG 2 cut(s) 32, 136
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 86, 292
CseI GACGC 1 cut(s) 395
CspCI CAANNNNNGTGG 2 cut(s) 28, 63
CviAII CATG 1 cut(s) 61
CviJI RGCY 8 cut(s) 31, 106, 120, 127, 167, 293, 398, 449
CviKI_1 RGCY 8 cut(s) 31, 106, 120, 127, 167, 293, 398, 449
DdeI CTNAG 2 cut(s) 109, 182
DpnI GATC 2 cut(s) 421, 474
DpnII GATC 2 cut(s) 419, 472
EaeI YGGCCR 1 cut(s) 396
Ecl136II GAGCTC 1 cut(s) 449
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 86
Eco53kI GAGCTC 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 294
EcoICRI GAGCTC 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 413
FaeI CATG 1 cut(s) 64
FaiI YATR 7 cut(s) 62, 130, 164, 354, 369, 509, 520
FaqI GGGAC 2 cut(s) 99, 447
FatI CATG 1 cut(s) 60
FauI CCCGC 2 cut(s) 81, 255
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 205
FokI GGATG 2 cut(s) 19, 123
FriOI GRGCYC 1 cut(s) 451
Fsp4HI GCNGC 1 cut(s) 205
FspBI CTAG 1 cut(s) 333
GlaI GCGC 1 cut(s) 227
GluI GCNGC 1 cut(s) 205
HaeIII GGCC 3 cut(s) 120, 293, 398
HapII CCGG 2 cut(s) 276, 399
HgaI GACGC 1 cut(s) 395
HhaI GCGC 1 cut(s) 228
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 1 cut(s) 64
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 1 cut(s) 494
HpaII CCGG 2 cut(s) 276, 399
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 2 cut(s) 158, 440
Hpy188I TCNGA 3 cut(s) 261, 287, 493
Hpy188III TCNNGA 1 cut(s) 423
Hpy8I GTNNAC 2 cut(s) 158, 440
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 2 cut(s) 98, 436
HpyCH4V TGCA 7 cut(s) 136, 233, 311, 363, 371, 383, 500
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 2 cut(s) 109, 182
HpySE526I ACGT 2 cut(s) 98, 436
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 1 cut(s) 64
HspAI GCGC 1 cut(s) 226
KflI GGGWCCC 1 cut(s) 86
Kzo9I GATC 2 cut(s) 419, 472
LmnI GCTCC 1 cut(s) 446
LpnPI CCDG 4 cut(s) 107, 134, 289, 412
Lsp1109I GCAGC 1 cut(s) 216
LweI GCATC 2 cut(s) 145, 509
MaeI CTAG 1 cut(s) 333
MaeII ACGT 2 cut(s) 98, 436
MaeIII GTNAC 3 cut(s) 55, 169, 452
MalI GATC 2 cut(s) 421, 474
MboI GATC 2 cut(s) 419, 472
MboII GAAGA 1 cut(s) 429
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 451
MluCI AATT 4 cut(s) 280, 328, 364, 478
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 1 cut(s) 471
MnlI CCTC 5 cut(s) 110, 138, 290, 463, 507
Mph1103I ATGCAT 1 cut(s) 373
MseI TTAA 2 cut(s) 327, 413
MspCI CTTAAG 1 cut(s) 412
MspI CCGG 2 cut(s) 276, 399
MspR9I CCNGG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 1 cut(s) 133
NdeII GATC 2 cut(s) 419, 472
NlaIII CATG 1 cut(s) 64
NlaIV GGNNCC 3 cut(s) 87, 88, 274
NmuCI GTSAC 2 cut(s) 55, 169
NsiI ATGCAT 1 cut(s) 373
PctI GAATGC 1 cut(s) 385
PkrI GCNGC 1 cut(s) 206
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
PpuMI RGGWCCY 1 cut(s) 86
Psp124BI GAGCTC 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 3 cut(s) 87, 88, 274
PspPI GGNCC 2 cut(s) 86, 292
PspPPI RGGWCCY 1 cut(s) 86
PsuI RGATCY 1 cut(s) 472
SacI GAGCTC 1 cut(s) 451
SaqAI TTAA 2 cut(s) 327, 413
SatI GCNGC 1 cut(s) 205
Sau3AI GATC 2 cut(s) 419, 472
Sau96I GGNCC 2 cut(s) 86, 292
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 1 cut(s) 451
SetI ASST 9 cut(s) 44, 101, 108, 115, 176, 271, 432, 439, 451
SfaNI GCATC 2 cut(s) 145, 509
SinI GGWCC 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
Sse9I AATT 4 cut(s) 280, 328, 364, 478
SsiI CCGC 2 cut(s) 74, 248
SspMI CTAG 1 cut(s) 333
SstI GAGCTC 1 cut(s) 451
StyD4I CCNGG 1 cut(s) 120
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 2 cut(s) 101, 439
TaqI TCGA 1 cut(s) 422
TasI AATT 4 cut(s) 280, 328, 364, 478
Tru1I TTAA 2 cut(s) 327, 413
Tru9I TTAA 2 cut(s) 327, 413
TscAI CASTG 1 cut(s) 240
TseFI GTSAC 2 cut(s) 55, 169
TseI GCWGC 1 cut(s) 204
Tsp45I GTSAC 2 cut(s) 55, 169
TspDTI ATGAA 1 cut(s) 404
TspRI CASTG 1 cut(s) 240
Vha464I CTTAAG 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 2 cut(s) 280, 478
XmiI GTMKAC 1 cut(s) 157
XspI CTAG 1 cut(s) 333
ZraI GACGTC 1 cut(s) 437
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.