Rh6DG018600

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0006047
Physical Location & Seq
Reverse (-)
1696836 .. 1698074
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG018600.1

Sequence Viewer

Length: 573 bp
ATGGAGAAGGTAGTTCTAAAGCTTTTCTTCATAGCTCTGTTCATGTTCATGCCATTACTACACAGTAGTAGCATTGAAGAAAATATAGAATTGGACCAATGGTTTCAGAAGCTCGACAACTCAAAACCGAAGCTGACCCGATTCCACTTCTACTTTCACGACATCGTTGCAGGCCCCGGCCAGACATCAGTCATAGCGGTCCCACCACCCAAGACCATCAAGCCATCCACCACCATGTTTGGACAAGTCAGTGTGTTTGACAACCCGCTAACCAAGGGACCCGAACTCACATCCGAGCTTGTAGGCAGGGGTCAGGGGCTATACAGTTACGCGTCCCAAGAAGAATTCAGTCTACTTCTGGCCATGACCATTGTTTTCACCGGCGGCAAAAACAACGGTAGCAGTGTCACTATTTTGGGGCGCAACTCAATGTTTCAATCCCGGCGAGAGTTTCCAATTGTCGGCGGAACTGGTGATTTCCGGTTGGCTCGTGGATTTGCGTCGGCCTCGACCTATATTGCTAATGCCAGTCTTGCTATTGTGGAATATAATGTTACGGTTATACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

190

Amino Acids

21.07

Weight (kDa)

7.97

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 46 - 187 1.6e-52 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 352
AccII CGCG 1 cut(s) 332
AciI CCGC 4 cut(s) 197, 266, 384, 465
AcoI YGGCCR 2 cut(s) 178, 360
AcsI RAATTY 1 cut(s) 344
AfiI CCNNNNNNNGG 1 cut(s) 461
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 2 cut(s) 77, 437
AluBI AGCT 5 cut(s) 22, 35, 112, 133, 298
AluI AGCT 5 cut(s) 22, 35, 112, 133, 298
AoxI GGCC 4 cut(s) 172, 178, 360, 504
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 4 cut(s) 94, 173, 199, 278
AsuC2I CCSGG 2 cut(s) 177, 442
AsuHPI GGTGA 2 cut(s) 370, 485
AvaII GGWCC 3 cut(s) 94, 199, 278
BalI TGGCCA 1 cut(s) 362
BauI CACGAG 1 cut(s) 489
BccI CCATC 2 cut(s) 224, 232
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BcnI CCSGG 2 cut(s) 177, 442
BisI GCNGC 1 cut(s) 385
BlsI GCNGC 1 cut(s) 386
Bme1390I CCNGG 2 cut(s) 177, 442
Bme18I GGWCC 3 cut(s) 94, 199, 278
BmgT120I GGNCC 4 cut(s) 94, 173, 199, 278
BmiI GGNNCC 4 cut(s) 175, 201, 279, 280
BmrFI CCNGG 2 cut(s) 177, 442
BoxI GACNNNNGTC 1 cut(s) 188
BpuMI CCSGG 2 cut(s) 177, 442
BsaJI CCNNGG 2 cut(s) 175, 273
BsaWI WCCGGW 1 cut(s) 480
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse118I RCCGGY 1 cut(s) 380
Bse1I ACTGG 2 cut(s) 475, 528
BseDI CCNNGG 2 cut(s) 175, 273
BseGI GGATG 2 cut(s) 224, 290
BseLI CCNNNNNNNGG 1 cut(s) 461
BseNI ACTGG 2 cut(s) 475, 528
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 4 cut(s) 174, 180, 362, 506
BsiSI CCGG 4 cut(s) 177, 381, 442, 481
BslFI GGGAC 3 cut(s) 185, 291, 319
BslI CCNNNNNNNGG 1 cut(s) 461
BsmFI GGGAC 3 cut(s) 185, 291, 319
BsnI GGCC 4 cut(s) 174, 180, 362, 506
BspACI CCGC 4 cut(s) 197, 266, 384, 465
BspANI GGCC 4 cut(s) 174, 180, 362, 506
BspFNI CGCG 1 cut(s) 332
BspLI GGNNCC 4 cut(s) 175, 201, 279, 280
BsrFI RCCGGY 1 cut(s) 380
BsrI ACTGG 2 cut(s) 475, 528
BssAI RCCGGY 1 cut(s) 380
BssECI CCNNGG 2 cut(s) 175, 273
BssSI CACGAG 1 cut(s) 489
BssT1I CCWWGG 1 cut(s) 273
Bst2BI CACGAG 1 cut(s) 489
Bst4CI ACNGT 4 cut(s) 65, 326, 398, 559
BstC8I GCNNGC 1 cut(s) 172
BstF5I GGATG 2 cut(s) 224, 290
BstFNI CGCG 1 cut(s) 332
BstHHI GCGC 1 cut(s) 423
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 175, 440
BstUI CGCG 1 cut(s) 332
BsuRI GGCC 4 cut(s) 174, 180, 362, 506
BtsCI GGATG 2 cut(s) 224, 290
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 256, 409
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 1 cut(s) 423
Cfr10I RCCGGY 1 cut(s) 380
Cfr13I GGNCC 4 cut(s) 94, 173, 199, 278
CseI GACGC 2 cut(s) 321, 489
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviAII CATG 4 cut(s) 43, 49, 235, 364
EaeI YGGCCR 2 cut(s) 178, 360
EciI GGCGGA 1 cut(s) 480
Eco130I CCWWGG 1 cut(s) 273
Eco47I GGWCC 3 cut(s) 94, 199, 278
EcoO109I RGGNCCY 2 cut(s) 173, 278
EcoRI GAATTC 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 273
FaeI CATG 4 cut(s) 46, 52, 238, 367
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 3 cut(s) 185, 291, 319
FatI CATG 4 cut(s) 42, 48, 234, 363
FauI CCCGC 1 cut(s) 273
FblI GTMKAC 1 cut(s) 352
Fnu4HI GCNGC 1 cut(s) 385
FokI GGATG 2 cut(s) 211, 277
Fsp4HI GCNGC 1 cut(s) 385
GlaI GCGC 1 cut(s) 422
GluI GCNGC 1 cut(s) 385
HaeIII GGCC 4 cut(s) 174, 180, 362, 506
HapII CCGG 4 cut(s) 177, 381, 442, 481
HgaI GACGC 2 cut(s) 321, 489
HhaI GCGC 1 cut(s) 423
Hin1II CATG 4 cut(s) 46, 52, 238, 367
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 1 cut(s) 141
HpaII CCGG 4 cut(s) 177, 381, 442, 481
HphI GGTGA 2 cut(s) 370, 485
Hpy166II GTNNAC 1 cut(s) 353
Hpy188I TCNGA 2 cut(s) 108, 295
Hpy188III TCNNGA 1 cut(s) 158
Hpy8I GTNNAC 1 cut(s) 353
Hpy99I CGWCG 1 cut(s) 505
HpyCH4III ACNGT 4 cut(s) 65, 326, 398, 559
HpyCH4V TGCA 1 cut(s) 170
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
Hsp92II CATG 4 cut(s) 46, 52, 238, 367
HspAI GCGC 1 cut(s) 421
KflI GGGWCCC 1 cut(s) 278
MaeIII GTNAC 3 cut(s) 326, 406, 553
MboII GAAGA 3 cut(s) 19, 89, 353
MfeI CAATTG 1 cut(s) 456
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 3 cut(s) 89, 344, 456
MluI ACGCGT 1 cut(s) 330
MluNI TGGCCA 1 cut(s) 362
MnlI CCTC 1 cut(s) 517
Mox20I TGGCCA 1 cut(s) 362
MscI TGGCCA 1 cut(s) 362
MslI CAYNNNNRTG 2 cut(s) 47, 233
Msp20I TGGCCA 1 cut(s) 362
MspI CCGG 4 cut(s) 177, 381, 442, 481
MspR9I CCNGG 2 cut(s) 177, 442
MunI CAATTG 1 cut(s) 456
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 1 cut(s) 533
NciI CCSGG 2 cut(s) 177, 442
NlaIII CATG 4 cut(s) 46, 52, 238, 367
NlaIV GGNNCC 4 cut(s) 175, 201, 279, 280
NmuCI GTSAC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 141
PkrI GCNGC 1 cut(s) 386
PpuMI RGGWCCY 1 cut(s) 278
PshAI GACNNNNGTC 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 278
PspN4I GGNNCC 4 cut(s) 175, 201, 279, 280
PspPI GGNCC 4 cut(s) 94, 173, 199, 278
PspPPI RGGWCCY 1 cut(s) 278
RseI CAYNNNNRTG 2 cut(s) 47, 233
SatI GCNGC 1 cut(s) 385
Sau96I GGNCC 4 cut(s) 94, 173, 199, 278
ScrFI CCNGG 2 cut(s) 177, 442
SetI ASST 7 cut(s) 12, 24, 37, 114, 135, 300, 515
SgrAI CRCCGGYG 1 cut(s) 380
SinI GGWCC 3 cut(s) 94, 199, 278
SmiMI CAYNNNNRTG 2 cut(s) 47, 233
Sse9I AATT 3 cut(s) 89, 344, 456
SsiI CCGC 4 cut(s) 197, 266, 384, 465
StyD4I CCNGG 2 cut(s) 175, 440
StyI CCWWGG 1 cut(s) 273
TaaI ACNGT 4 cut(s) 65, 326, 398, 559
TaqI TCGA 2 cut(s) 114, 509
TasI AATT 3 cut(s) 89, 344, 456
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 1 cut(s) 141
TscAI CASTG 2 cut(s) 256, 409
TseFI GTSAC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 3 cut(s) 19, 31, 37
TspRI CASTG 2 cut(s) 256, 409
VpaK11BI GGWCC 3 cut(s) 94, 199, 278
XapI RAATTY 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.