Rh6DG018500

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0006047
Physical Location & Seq
Reverse (-)
1693670 .. 1694288
619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG018500.1

Sequence Viewer

Length: 567 bp
ATGGAGAAGGTAGTTCCAAAGCTTTTCTTCATAGCTCTGTTCATGTTCATACCATTACTAGGGCACTGTAGCAGCATCGAAGAAAATAGAGAATTTGACCAATGGTTTCAGAAGCTCGACAACTCAAAACCGAAGCTGACCCAATTCCACTTCTACTTTCACGACCTTGTTTCAGGCCCCGGCCAGACAGCAGTCGTAGTTGTTCCACCAGCCAAGCCATCGACCACATTGTTCGGACAAGTCAACATGTTTGACAACCCGCTAACCAAGGGACCCGAACTCACGTCCGAGCTTGTAGGCAGGGCTCAGGGGCTATACAGTTTCGCGTCCCAAGAAGAGCTCTCTCTACTTATGGCCATGACCATTGTTTTCACCAGCGGCAAACACAACGGTAGCAGTGTCACTATTTTGGGGCGCAACGCAGTGTTTCAATCCCGGCGAGAGTTTCCAATTGTCGGCGGAACTGGCGATTTCCGGTTGGCCCGTGGATTTGCGTCAGTCTCGAGCTATTTTATTAATGCTACTCTTGGTATTCTGGAATATAATCTTATGGTTATACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

188

Amino Acids

20.98

Weight (kDa)

6.97

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 47 - 185 2.6e-52 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 326
AciI CCGC 3 cut(s) 260, 378, 459
AcoI YGGCCR 2 cut(s) 181, 354
AcsI RAATTY 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 59, 455
AflIII ACRYGT 1 cut(s) 246
AgsI TTSAA 1 cut(s) 431
AjiI CACGTC 1 cut(s) 285
AluBI AGCT 7 cut(s) 22, 35, 115, 136, 292, 340, 507
AluI AGCT 7 cut(s) 22, 35, 115, 136, 292, 340, 507
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 505
Ama87I CYCGRG 1 cut(s) 502
AoxI GGCC 4 cut(s) 175, 181, 354, 480
ApeKI GCWGC 1 cut(s) 72
ApoI RAATTY 1 cut(s) 92
AseI ATTAAT 1 cut(s) 516
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 3 cut(s) 176, 272, 481
AsuC2I CCSGG 2 cut(s) 180, 436
AsuHPI GGTGA 1 cut(s) 364
AvaI CYCGRG 1 cut(s) 502
AvaII GGWCC 1 cut(s) 272
BaeGI GKGCMC 1 cut(s) 66
BalI TGGCCA 1 cut(s) 356
BanII GRGCYC 2 cut(s) 307, 342
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 226
BcnI CCSGG 2 cut(s) 180, 436
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 67
BisI GCNGC 2 cut(s) 73, 379
BlsI GCNGC 2 cut(s) 74, 380
Bme1390I CCNGG 2 cut(s) 180, 436
Bme18I GGWCC 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 502
BmgBI CACGTC 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 176, 272, 481
BmiI GGNNCC 3 cut(s) 178, 273, 274
BmrFI CCNGG 2 cut(s) 180, 436
BmsI GCATC 1 cut(s) 84
BoxI GACNNNNGTC 1 cut(s) 191
Bpu10I CCTNAGC 1 cut(s) 306
BpuMI CCSGG 2 cut(s) 180, 436
BsaJI CCNNGG 3 cut(s) 178, 267, 484
BsaWI WCCGGW 1 cut(s) 474
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 455
Bse1I ACTGG 1 cut(s) 469
BseDI CCNNGG 3 cut(s) 178, 267, 484
BseLI CCNNNNNNNGG 2 cut(s) 59, 455
BseMII CTCAG 1 cut(s) 320
BseNI ACTGG 1 cut(s) 469
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 4 cut(s) 177, 183, 356, 482
BsiHKAI GWGCWC 1 cut(s) 342
BsiHKCI CYCGRG 1 cut(s) 502
BsiSI CCGG 3 cut(s) 180, 436, 475
BslFI GGGAC 2 cut(s) 285, 313
BslI CCNNNNNNNGG 2 cut(s) 59, 455
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 285, 313
BsnI GGCC 4 cut(s) 177, 183, 356, 482
BsoBI CYCGRG 1 cut(s) 502
Bsp1286I GDGCHC 3 cut(s) 66, 307, 342
BspACI CCGC 3 cut(s) 260, 378, 459
BspANI GGCC 4 cut(s) 177, 183, 356, 482
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 3 cut(s) 178, 273, 274
BspQI GCTCTTC 1 cut(s) 330
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 178, 267, 484
BssT1I CCWWGG 1 cut(s) 267
Bst4CI ACNGT 3 cut(s) 68, 320, 392
Bst6I CTCTTC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 484
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 417
BstMAI GTCTC 1 cut(s) 505
BstMWI GCNNNNNNNGC 1 cut(s) 465
BstNSI RCATGY 1 cut(s) 250
BstPAI GACNNNNGTC 1 cut(s) 191
BstSCI CCNGG 2 cut(s) 178, 434
BstSFI CTRYAG 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 66
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 1 cut(s) 84
BsuRI GGCC 4 cut(s) 177, 183, 356, 482
BtgI CCRYGG 1 cut(s) 484
BtrI CACGTC 1 cut(s) 285
BtsI GCAGTG 2 cut(s) 403, 429
BtsIMutI CAGTG 3 cut(s) 64, 403, 429
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 3 cut(s) 176, 272, 481
CseI GACGC 2 cut(s) 315, 483
CviAII CATG 3 cut(s) 43, 247, 358
DdeI CTNAG 1 cut(s) 306
EaeI YGGCCR 2 cut(s) 181, 354
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 474
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 2 cut(s) 307, 342
Eco47I GGWCC 1 cut(s) 272
Eco53kI GAGCTC 1 cut(s) 340
Eco88I CYCGRG 1 cut(s) 502
EcoICRI GAGCTC 1 cut(s) 340
EcoO109I RGGNCCY 2 cut(s) 176, 272
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 2 cut(s) 307, 342
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 3 cut(s) 46, 250, 361
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 2 cut(s) 285, 313
FatI CATG 3 cut(s) 42, 246, 357
FauI CCCGC 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 73, 379
FriOI GRGCYC 2 cut(s) 307, 342
Fsp4HI GCNGC 2 cut(s) 73, 379
FspBI CTAG 1 cut(s) 59
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 73, 379
HaeIII GGCC 4 cut(s) 177, 183, 356, 482
HapII CCGG 3 cut(s) 180, 436, 475
HgaI GACGC 2 cut(s) 315, 483
HhaI GCGC 1 cut(s) 417
Hin1II CATG 3 cut(s) 46, 250, 361
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 20
HpaII CCGG 3 cut(s) 180, 436, 475
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 3 cut(s) 111, 236, 289
Hpy188III TCNNGA 3 cut(s) 161, 502, 536
Hpy8I GTNNAC 1 cut(s) 244
HpyCH4III ACNGT 3 cut(s) 68, 320, 392
HpyCH4IV ACGT 1 cut(s) 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 465
HpyF3I CTNAG 1 cut(s) 306
HpySE526I ACGT 1 cut(s) 284
Hsp92II CATG 3 cut(s) 46, 250, 361
HspAI GCGC 1 cut(s) 415
KflI GGGWCCC 1 cut(s) 272
LguI GCTCTTC 1 cut(s) 330
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 1 cut(s) 84
MaeI CTAG 1 cut(s) 59
MaeII ACGT 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 400
MboII GAAGA 3 cut(s) 19, 92, 347
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 3 cut(s) 66, 307, 342
MlsI TGGCCA 1 cut(s) 356
MluCI AATT 3 cut(s) 92, 143, 450
MluNI TGGCCA 1 cut(s) 356
Mox20I TGGCCA 1 cut(s) 356
MscI TGGCCA 1 cut(s) 356
MseI TTAA 1 cut(s) 516
Msp20I TGGCCA 1 cut(s) 356
MspA1I CMGCKG 1 cut(s) 378
MspI CCGG 3 cut(s) 180, 436, 475
MspR9I CCNGG 2 cut(s) 180, 436
MunI CAATTG 1 cut(s) 450
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 465
NciI CCSGG 2 cut(s) 180, 436
NlaIII CATG 3 cut(s) 46, 250, 361
NlaIV GGNNCC 3 cut(s) 178, 273, 274
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 1 cut(s) 250
PaeR7I CTCGAG 1 cut(s) 502
PciI ACATGT 1 cut(s) 246
PciSI GCTCTTC 1 cut(s) 330
PkrI GCNGC 2 cut(s) 74, 380
PpuMI RGGWCCY 1 cut(s) 272
PscI ACATGT 1 cut(s) 246
PshAI GACNNNNGTC 1 cut(s) 191
PshBI ATTAAT 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 272
PspN4I GGNNCC 3 cut(s) 178, 273, 274
PspPI GGNCC 3 cut(s) 176, 272, 481
PspPPI RGGWCCY 1 cut(s) 272
SacI GAGCTC 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 2 cut(s) 73, 379
Sau96I GGNCC 3 cut(s) 176, 272, 481
ScrFI CCNGG 2 cut(s) 180, 436
SduI GDGCHC 3 cut(s) 66, 307, 342
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 1 cut(s) 67
Sfr274I CTCGAG 1 cut(s) 502
SinI GGWCC 1 cut(s) 272
SlaI CTCGAG 1 cut(s) 502
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
Sse9I AATT 3 cut(s) 92, 143, 450
SsiI CCGC 3 cut(s) 260, 378, 459
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 178, 434
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 3 cut(s) 68, 320, 392
TaiI ACGT 1 cut(s) 287
TaqI TCGA 4 cut(s) 78, 117, 221, 503
TasI AATT 3 cut(s) 92, 143, 450
TauI GCSGC 1 cut(s) 381
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 3 cut(s) 71, 403, 429
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 3 cut(s) 19, 31, 37
TspRI CASTG 3 cut(s) 71, 403, 429
VpaK11BI GGWCC 1 cut(s) 272
VspI ATTAAT 1 cut(s) 516
XapI RAATTY 1 cut(s) 92
XceI RCATGY 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 502
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.