Rmu_sc0003679.1_g000008

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003679.1
OG0006047
Physical Location & Seq
Forward (+)
24824 .. 25390
567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003679.1_g000008.1.cds

Sequence Viewer

Length: 567 bp
atggagaaggtagttccaaagcttttcttcatagctctgttcatgctcataccattactagggcactgtagcagcatcgaagaaaatagagaatttgaccaatggtttcagaagctcgacaactcaaaacccaagctgacccgattccacttctactttcacgacatcgtttcaggctatggccagacatcattcgtagtggttccaccacccaagatcatcaagccatcgaccaccttgttcggacaagtcaacatatttgacaacccactacccgaactcacgtccgaccttgtaggtagggctcaggggctatacagtttcgcgtcccaagaagagctctgtctacttatggccatgaccattgttttcaccagcggcaaacacaacggtagcggtgtcactattttggggcgcaacgcagtgtttcaatcccggcgagagtttccaattgtcggcggaactggcgatttccggttggcccgtggatttgcgtcagtctccagctattttgttaatgctactcttggtattctggaatataatcttatggttatacattattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

188

Amino Acids

21.14

Weight (kDa)

7.81

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 346
AccII CGCG 1 cut(s) 326
AciI CCGC 3 cut(s) 378, 396, 459
AcoI YGGCCR 2 cut(s) 181, 354
AcsI RAATTY 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 59, 455
AgsI TTSAA 1 cut(s) 431
AjiI CACGTC 1 cut(s) 285
AluBI AGCT 6 cut(s) 22, 35, 115, 136, 340, 507
AluI AGCT 6 cut(s) 22, 35, 115, 136, 340, 507
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 505
AoxI GGCC 3 cut(s) 181, 354, 480
ApeKI GCWGC 1 cut(s) 72
ApoI RAATTY 1 cut(s) 92
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 1 cut(s) 481
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 1 cut(s) 364
BaeGI GKGCMC 1 cut(s) 66
BalI TGGCCA 2 cut(s) 183, 356
BanII GRGCYC 2 cut(s) 307, 342
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 1 cut(s) 84
BccI CCATC 1 cut(s) 235
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 67
BisI GCNGC 2 cut(s) 73, 379
BlsI GCNGC 2 cut(s) 74, 380
Bme1390I CCNGG 1 cut(s) 436
BmgBI CACGTC 1 cut(s) 285
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 487
Bpu10I CCTNAGC 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 436
BsaJI CCNNGG 1 cut(s) 484
BsaWI WCCGGW 1 cut(s) 474
Bsc4I CCNNNNNNNGG 2 cut(s) 59, 455
Bse1I ACTGG 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 484
BseLI CCNNNNNNNGG 2 cut(s) 59, 455
BseMII CTCAG 1 cut(s) 320
BseNI ACTGG 1 cut(s) 469
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 1 cut(s) 84
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 3 cut(s) 183, 356, 482
BsiHKAI GWGCWC 1 cut(s) 342
BsiSI CCGG 2 cut(s) 436, 475
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 2 cut(s) 59, 455
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 3 cut(s) 183, 356, 482
Bsp1286I GDGCHC 3 cut(s) 66, 307, 342
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 3 cut(s) 378, 396, 459
BspANI GGCC 3 cut(s) 183, 356, 482
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 1 cut(s) 204
BspQI GCTCTTC 1 cut(s) 330
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 1 cut(s) 484
BssMI GATC 1 cut(s) 216
Bst4CI ACNGT 3 cut(s) 68, 320, 392
Bst6I CTCTTC 1 cut(s) 330
BstDEI CTNAG 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 484
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 1 cut(s) 465
BstSCI CCNGG 1 cut(s) 434
BstSFI CTRYAG 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 66
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 1 cut(s) 84
BsuRI GGCC 3 cut(s) 183, 356, 482
BtgI CCRYGG 1 cut(s) 484
BtrI CACGTC 1 cut(s) 285
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 64, 429
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 1 cut(s) 481
CseI GACGC 2 cut(s) 315, 483
CviAII CATG 2 cut(s) 43, 358
DdeI CTNAG 1 cut(s) 306
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
EaeI YGGCCR 2 cut(s) 181, 354
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 474
Ecl136II GAGCTC 1 cut(s) 340
Eco24I GRGCYC 2 cut(s) 307, 342
Eco53kI GAGCTC 1 cut(s) 340
EcoICRI GAGCTC 1 cut(s) 340
EcoT38I GRGCYC 2 cut(s) 307, 342
FaeI CATG 2 cut(s) 46, 361
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 313
FatI CATG 2 cut(s) 42, 357
FblI GTMKAC 1 cut(s) 346
Fnu4HI GCNGC 2 cut(s) 73, 379
FriOI GRGCYC 2 cut(s) 307, 342
Fsp4HI GCNGC 2 cut(s) 73, 379
FspBI CTAG 1 cut(s) 59
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 73, 379
GsuI CTGGAG 1 cut(s) 487
HaeIII GGCC 3 cut(s) 183, 356, 482
HapII CCGG 2 cut(s) 436, 475
HgaI GACGC 2 cut(s) 315, 483
HhaI GCGC 1 cut(s) 417
Hin1II CATG 2 cut(s) 46, 361
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 1 cut(s) 144
HpaII CCGG 2 cut(s) 436, 475
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 2 cut(s) 253, 347
Hpy188I TCNGA 3 cut(s) 111, 245, 289
Hpy188III TCNNGA 2 cut(s) 161, 536
Hpy8I GTNNAC 2 cut(s) 253, 347
HpyCH4III ACNGT 3 cut(s) 68, 320, 392
HpyCH4IV ACGT 1 cut(s) 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 465
HpyF3I CTNAG 1 cut(s) 306
HpySE526I ACGT 1 cut(s) 284
Hsp92II CATG 2 cut(s) 46, 361
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 1 cut(s) 216
LguI GCTCTTC 1 cut(s) 330
LpnPI CCDG 9 cut(s) 159, 197, 293, 388, 449, 450, 488, 517, 521
Lsp1109I GCAGC 1 cut(s) 84
LweI GCATC 1 cut(s) 84
MaeI CTAG 1 cut(s) 59
MaeII ACGT 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 400
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 3 cut(s) 19, 92, 347
MfeI CAATTG 1 cut(s) 450
MhlI GDGCHC 3 cut(s) 66, 307, 342
MlsI TGGCCA 2 cut(s) 183, 356
MluCI AATT 2 cut(s) 92, 450
MluNI TGGCCA 2 cut(s) 183, 356
MmeI TCCRAC 1 cut(s) 312
Mox20I TGGCCA 2 cut(s) 183, 356
MscI TGGCCA 2 cut(s) 183, 356
MseI TTAA 1 cut(s) 516
Msp20I TGGCCA 2 cut(s) 183, 356
MspA1I CMGCKG 1 cut(s) 378
MspI CCGG 2 cut(s) 436, 475
MspR9I CCNGG 1 cut(s) 436
MunI CAATTG 1 cut(s) 450
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 465
NciI CCSGG 1 cut(s) 436
NdeII GATC 1 cut(s) 216
NlaIII CATG 2 cut(s) 46, 361
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 400
PciSI GCTCTTC 1 cut(s) 330
PfeI GAWTC 1 cut(s) 144
PkrI GCNGC 2 cut(s) 74, 380
Psp124BI GAGCTC 1 cut(s) 342
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 481
SacI GAGCTC 1 cut(s) 342
SapI GCTCTTC 1 cut(s) 330
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 2 cut(s) 73, 379
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 1 cut(s) 481
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 3 cut(s) 66, 307, 342
SfaNI GCATC 1 cut(s) 84
SfcI CTRYAG 1 cut(s) 67
Sse9I AATT 2 cut(s) 92, 450
SsiI CCGC 3 cut(s) 378, 396, 459
SspMI CTAG 1 cut(s) 59
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 434
TaaI ACNGT 3 cut(s) 68, 320, 392
TaiI ACGT 1 cut(s) 287
TaqI TCGA 3 cut(s) 78, 117, 230
TasI AATT 2 cut(s) 92, 450
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 2 cut(s) 71, 429
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 2 cut(s) 19, 31
TspRI CASTG 2 cut(s) 71, 429
XapI RAATTY 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 346
XspI CTAG 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.