MD10G1120700.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006047
Physical Location & Seq
Forward (+)
19733498 .. 19734079
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1120700.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGAAAAGGTCGGTTCAATAATATTTCTCAACCATTTGTTCATAATATGCCTGGCCGCGCCATTAATGGTACAGAGCACCATTGAAAACCCTAGGGAGAACATTGACCAATGGTTCCAAAACCTCCCCCATGCAAAACAGAAGCTGACCAGGCTGCATTTCTACTTCCACGACGTCGTTCTCGGCCCACACCCAACCGTAGTGGCGGTGGCCAACGCATCCCATACCTTCACATCACCCACCTTCTTCGGCCGAGTCAACATCTTCGACAACCCCTTGACCCAGGGACCCGAACCCGGTTCCAAGCTCGTGGGTCGGGCGCAAGGGCTATATGGGTTTACCTCACAGGAGGAAGTGAGCCTGCTTATGGCTATGAACTTTGTTTTCAGGAGTTGGAAGCACAACGGCAGCAGCCTCACCGTTTTGGGGAGGAACCCGGTTGGGCAGTTGGTTAGAGAGTTGCCCATCGTCGGCGGAACCGGTGTTTTTCGGTTGGCACGCGGATTTGTGTTGGCCAAGACTTATTTCTTTAATGCTAGTGTTGCCACTGTTGAATATAATGTTGCAGTTTTGCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

194

Amino Acids

21.5

Weight (kDa)

9.41

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 50 - 190 2.3e-52 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
AccII CGCG 2 cut(s) 59, 501
AciI CCGC 4 cut(s) 57, 206, 474, 501
AcoI YGGCCR 4 cut(s) 54, 210, 250, 513
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 1 cut(s) 72
AfiI CCNNNNNNNGG 4 cut(s) 296, 367, 426, 470
AgeI ACCGGT 1 cut(s) 479
AgsI TTSAA 3 cut(s) 18, 86, 554
AjnI CCWGG 3 cut(s) 51, 149, 282
AjuI GAANNNNNNNTTGG 2 cut(s) 509, 541
AluBI AGCT 2 cut(s) 145, 307
AluI AGCT 2 cut(s) 145, 307
Alw21I GWGCWC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 145
AoxI GGCC 5 cut(s) 54, 184, 210, 250, 513
ApeKI GCWGC 3 cut(s) 154, 408, 411
AseI ATTAAT 1 cut(s) 65
AsiGI ACCGGT 1 cut(s) 479
AspA2I CCTAGG 1 cut(s) 92
AspLEI GCGC 2 cut(s) 61, 322
AspS9I GGNCC 2 cut(s) 185, 287
AsuC2I CCSGG 2 cut(s) 297, 437
AsuHPI GGTGA 2 cut(s) 228, 409
AvaII GGWCC 1 cut(s) 287
AvrII CCTAGG 1 cut(s) 92
BalI TGGCCA 2 cut(s) 212, 515
BauI CACGAG 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 3 cut(s) 141, 420, 423
BccI CCATC 1 cut(s) 473
BceAI ACGGC 1 cut(s) 421
BciT130I CCWGG 3 cut(s) 53, 151, 284
BcnI CCSGG 2 cut(s) 297, 437
BfaI CTAG 2 cut(s) 93, 537
BisI GCNGC 4 cut(s) 57, 155, 409, 412
BlnI CCTAGG 1 cut(s) 92
BlsI GCNGC 4 cut(s) 58, 156, 410, 413
Bme1390I CCNGG 5 cut(s) 53, 151, 284, 297, 437
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 2 cut(s) 185, 287
BmiI GGNNCC 6 cut(s) 116, 288, 289, 301, 434, 478
BmrFI CCNGG 5 cut(s) 53, 151, 284, 297, 437
BmsI GCATC 1 cut(s) 227
BpuMI CCSGG 2 cut(s) 297, 437
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 3 cut(s) 92, 282, 283
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 367, 426, 470
Bse118I RCCGGY 1 cut(s) 479
BseBI CCWGG 3 cut(s) 53, 151, 284
BseDI CCNNGG 3 cut(s) 92, 282, 283
BseGI GGATG 1 cut(s) 218
BseLI CCNNNNNNNGG 4 cut(s) 296, 367, 426, 470
BseX3I CGGCCG 1 cut(s) 250
BseXI GCAGC 3 cut(s) 141, 420, 423
Bsh1236I CGCG 2 cut(s) 59, 501
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 5 cut(s) 56, 186, 212, 252, 515
BshTI ACCGGT 1 cut(s) 479
BsiEI CGRYCG 1 cut(s) 253
BsiHKAI GWGCWC 1 cut(s) 80
BsiSI CCGG 3 cut(s) 297, 437, 480
BslFI GGGAC 1 cut(s) 300
BslI CCNNNNNNNGG 4 cut(s) 296, 367, 426, 470
BsmFI GGGAC 1 cut(s) 300
BsnI GGCC 5 cut(s) 56, 186, 212, 252, 515
Bsp1286I GDGCHC 1 cut(s) 80
BspACI CCGC 4 cut(s) 57, 206, 474, 501
BspANI GGCC 5 cut(s) 56, 186, 212, 252, 515
BspFNI CGCG 2 cut(s) 59, 501
BspLI GGNNCC 6 cut(s) 116, 288, 289, 301, 434, 478
BsrFI RCCGGY 1 cut(s) 479
BssAI RCCGGY 1 cut(s) 479
BssECI CCNNGG 3 cut(s) 92, 282, 283
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 1 cut(s) 92
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 3 cut(s) 53, 151, 284
Bst4CI ACNGT 3 cut(s) 199, 421, 550
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 2 cut(s) 362, 499
BstF5I GGATG 1 cut(s) 218
BstFNI CGCG 2 cut(s) 59, 501
BstHHI GCGC 2 cut(s) 61, 322
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 2 cut(s) 151, 542
BstNI CCWGG 3 cut(s) 53, 151, 284
BstSCI CCNGG 5 cut(s) 51, 149, 282, 295, 435
BstUI CGCG 2 cut(s) 59, 501
BstV1I GCAGC 3 cut(s) 141, 420, 423
BstXI CCANNNNNNTGG 1 cut(s) 310
BstZI CGGCCG 1 cut(s) 250
BsuRI GGCC 5 cut(s) 56, 186, 212, 252, 515
BtsCI GGATG 1 cut(s) 218
BtsIMutI CAGTG 1 cut(s) 546
Cac8I GCNNGC 2 cut(s) 362, 499
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 2 cut(s) 61, 322
Cfr10I RCCGGY 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 185, 287
Csp6I GTAC 1 cut(s) 71
CspAI ACCGGT 1 cut(s) 479
CspCI CAANNNNNGTGG 2 cut(s) 183, 218
CviAII CATG 1 cut(s) 131
CviQI GTAC 1 cut(s) 71
EaeI YGGCCR 4 cut(s) 54, 210, 250, 513
EagI CGGCCG 1 cut(s) 250
EciI GGCGGA 1 cut(s) 489
EclXI CGGCCG 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 287
Eco52I CGGCCG 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 3 cut(s) 51, 149, 282
EcoT14I CCWWGG 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 92
FaeI CATG 1 cut(s) 134
FaiI YATR 9 cut(s) 44, 49, 132, 225, 331, 333, 368, 374, 558
FaqI GGGAC 1 cut(s) 300
FatI CATG 1 cut(s) 130
Fnu4HI GCNGC 4 cut(s) 57, 155, 409, 412
FokI GGATG 1 cut(s) 205
Fsp4HI GCNGC 4 cut(s) 57, 155, 409, 412
FspBI CTAG 2 cut(s) 93, 537
GlaI GCGC 2 cut(s) 60, 321
GluI GCNGC 4 cut(s) 57, 155, 409, 412
HaeIII GGCC 5 cut(s) 56, 186, 212, 252, 515
HapII CCGG 3 cut(s) 297, 437, 480
HhaI GCGC 2 cut(s) 61, 322
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 1 cut(s) 134
Hin6I GCGC 2 cut(s) 59, 320
HinP1I GCGC 2 cut(s) 59, 320
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 1 cut(s) 255
HpaII CCGG 3 cut(s) 297, 437, 480
HphI GGTGA 2 cut(s) 228, 409
Hpy166II GTNNAC 2 cut(s) 259, 339
Hpy188III TCNNGA 1 cut(s) 388
Hpy8I GTNNAC 2 cut(s) 259, 339
Hpy99I CGWCG 3 cut(s) 176, 179, 473
HpyAV CCTTC 2 cut(s) 238, 253
HpyCH4III ACNGT 3 cut(s) 199, 421, 550
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 134, 157, 566, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 151, 542
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 1 cut(s) 134
HspAI GCGC 2 cut(s) 59, 320
KflI GGGWCCC 1 cut(s) 287
Lsp1109I GCAGC 3 cut(s) 141, 420, 423
LweI GCATC 1 cut(s) 227
MaeI CTAG 2 cut(s) 93, 537
MaeII ACGT 1 cut(s) 174
MboII GAAGA 2 cut(s) 238, 256
MhlI GDGCHC 1 cut(s) 80
MlsI TGGCCA 2 cut(s) 212, 515
MluNI TGGCCA 2 cut(s) 212, 515
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 1 cut(s) 374
MnlI CCTC 5 cut(s) 134, 343, 352, 423, 425
Mox20I TGGCCA 2 cut(s) 212, 515
MscI TGGCCA 2 cut(s) 212, 515
MseI TTAA 2 cut(s) 65, 531
Msp20I TGGCCA 2 cut(s) 212, 515
MspI CCGG 3 cut(s) 297, 437, 480
MspR9I CCNGG 5 cut(s) 53, 151, 284, 297, 437
MvaI CCWGG 3 cut(s) 53, 151, 284
MvnI CGCG 2 cut(s) 59, 501
MwoI GCNNNNNNNGC 2 cut(s) 151, 542
NciI CCSGG 2 cut(s) 297, 437
NlaIII CATG 1 cut(s) 134
NlaIV GGNNCC 6 cut(s) 116, 288, 289, 301, 434, 478
NmeAIII GCCGAG 2 cut(s) 162, 278
PasI CCCWGGG 1 cut(s) 283
PinAI ACCGGT 1 cut(s) 479
PkrI GCNGC 4 cut(s) 58, 156, 410, 413
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PpuMI RGGWCCY 1 cut(s) 287
PshBI ATTAAT 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 3 cut(s) 51, 149, 282
PspGI CCWGG 3 cut(s) 51, 149, 282
PspN4I GGNNCC 6 cut(s) 116, 288, 289, 301, 434, 478
PspPI GGNCC 2 cut(s) 185, 287
PspPPI RGGWCCY 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 145
RsaI GTAC 1 cut(s) 72
RsaNI GTAC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 65, 531
SatI GCNGC 4 cut(s) 57, 155, 409, 412
Sau96I GGNCC 2 cut(s) 185, 287
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 5 cut(s) 53, 151, 284, 297, 437
SduI GDGCHC 1 cut(s) 80
SetI ASST 8 cut(s) 12, 126, 147, 177, 230, 245, 309, 344
SfaNI GCATC 1 cut(s) 227
SinI GGWCC 1 cut(s) 287
SsiI CCGC 4 cut(s) 57, 206, 474, 501
SspI AATATT 1 cut(s) 24
SspMI CTAG 2 cut(s) 93, 537
StyD4I CCNGG 5 cut(s) 51, 149, 282, 295, 435
StyI CCWWGG 1 cut(s) 92
TaaI ACNGT 3 cut(s) 199, 421, 550
TaiI ACGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 267
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 2 cut(s) 65, 531
Tru9I TTAA 2 cut(s) 65, 531
TscAI CASTG 1 cut(s) 553
TseI GCWGC 3 cut(s) 154, 408, 411
TspDTI ATGAA 2 cut(s) 31, 389
TspRI CASTG 1 cut(s) 553
VpaK11BI GGWCC 1 cut(s) 287
VspI ATTAAT 1 cut(s) 65
XmaJI CCTAGG 1 cut(s) 92
XspI CTAG 2 cut(s) 93, 537
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.