FvH4_2g01680

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006047
Physical Location & Seq
Reverse (-)
1500537 .. 1501264
728 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01680.t1

Sequence Viewer

Length: 582 bp
ATGGGGAAGTTTTCAGGTCCAGTTCTAAAGCTTTTCTTCGTTGTTCTGTTCATGTCATTATCGGTGCACACCAGCAGCAACATTGAAGAGAAGAGAGAAACTGATCACTGGTTTCAAAGGCTCCACTACTCAAAACAGAAGCTGACCCGGCTCCATTTCTACTTCCACGACATAGTTTCCGGCCCGGCTCAGACCACAATGGTGGTGGTGCCACCAGCCAAGACCGGCAAACCATCTCACACGTCGTTCGGGCAGGTCAACATGTTTGACAACCCTCTAACCAAAGGACCTGAACCCACATCTGAGCTGCTTGGTCGTGCCCAAGGTTTATATGGGTTCGCATCCCATGAGGACGTCAGTCTACTTATGGCTATGACCATTGTTCTCACCGGTGGCGAACACAACGGTAGTAGCTTTACTGTTTTGGGCCGGAATGCGGTGATAGATTCTACCCGAGAGTTCCCAGTTGTCGGTGGAACTGGTGAATTTCGATTAGGACGTGGATTTGCGACAGCCAAGACTTACTTTTTTAACGACACTATTGCTATAGTGGAATATAATGTTGTGGTTATACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

194

Amino Acids

21.44

Weight (kDa)

8.04

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 49 - 190 7.5e-52 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 208
AccI GTMKAC 1 cut(s) 361
AciI CCGC 1 cut(s) 437
AcsI RAATTY 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 354
AfiI CCNNNNNNNGG 2 cut(s) 436, 470
AflIII ACRYGT 2 cut(s) 240, 261
AgeI ACCGGT 1 cut(s) 389
AgsI TTSAA 2 cut(s) 86, 116
AjiI CACGTC 2 cut(s) 243, 500
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 4 cut(s) 31, 142, 307, 414
AluI AGCT 4 cut(s) 31, 142, 307, 414
Alw21I GWGCWC 1 cut(s) 69
Alw44I GTGCAC 1 cut(s) 65
AlwNI CAGNNNCTG 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 453
AoxI GGCC 2 cut(s) 181, 427
ApaLI GTGCAC 1 cut(s) 65
ApeKI GCWGC 2 cut(s) 75, 307
ApoI RAATTY 1 cut(s) 485
AsiGI ACCGGT 1 cut(s) 389
AspS9I GGNCC 4 cut(s) 17, 182, 287, 427
AsuC2I CCSGG 2 cut(s) 148, 185
AsuHPI GGTGA 3 cut(s) 379, 451, 494
AvaI CYCGRG 1 cut(s) 453
AvaII GGWCC 2 cut(s) 17, 287
BaeGI GKGCMC 2 cut(s) 69, 322
BanI GGYRCC 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 69
BbvI GCAGC 2 cut(s) 87, 294
BccI CCATC 1 cut(s) 241
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 2 cut(s) 148, 185
BfmI CTRYAG 1 cut(s) 546
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 2 cut(s) 76, 308
BlsI GCNGC 2 cut(s) 77, 309
Bme1390I CCNGG 2 cut(s) 148, 185
Bme18I GGWCC 2 cut(s) 17, 287
BmeT110I CYCGRG 1 cut(s) 453
BmgBI CACGTC 2 cut(s) 243, 500
BmgT120I GGNCC 4 cut(s) 17, 182, 287, 427
BmiI GGNNCC 3 cut(s) 122, 152, 210
BmrFI CCNGG 2 cut(s) 148, 185
BmrI ACTGGG 1 cut(s) 458
BmsI GCATC 1 cut(s) 350
BmuI ACTGGG 1 cut(s) 458
BoxI GACNNNNGTC 1 cut(s) 357
BpuMI CCSGG 2 cut(s) 148, 185
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 1 cut(s) 322
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 470
Bse118I RCCGGY 2 cut(s) 224, 389
Bse1I ACTGG 4 cut(s) 20, 113, 464, 484
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 1 cut(s) 341
BseLI CCNNNNNNNGG 2 cut(s) 436, 470
BseMII CTCAG 2 cut(s) 203, 294
BseNI ACTGG 4 cut(s) 20, 113, 464, 484
BseSI GKGCMC 2 cut(s) 69, 322
BseXI GCAGC 2 cut(s) 87, 294
BshFI GGCC 2 cut(s) 183, 429
BshNI GGYRCC 1 cut(s) 208
BshTI ACCGGT 1 cut(s) 389
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 453
BsiSI CCGG 6 cut(s) 148, 180, 185, 225, 390, 430
BslI CCNNNNNNNGG 2 cut(s) 436, 470
BsmI GAATGC 1 cut(s) 439
BsnI GGCC 2 cut(s) 183, 429
BsoBI CYCGRG 1 cut(s) 453
Bsp1286I GDGCHC 2 cut(s) 69, 322
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 1 cut(s) 437
BspANI GGCC 2 cut(s) 183, 429
BspCNI CTCAG 2 cut(s) 202, 295
BspLI GGNNCC 3 cut(s) 122, 152, 210
BspMI ACCTGC 1 cut(s) 244
BspT107I GGYRCC 1 cut(s) 208
BsrFI RCCGGY 2 cut(s) 224, 389
BsrI ACTGG 4 cut(s) 20, 113, 464, 484
BssAI RCCGGY 2 cut(s) 224, 389
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 1 cut(s) 103
BssNI GRCGYC 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 322
Bst4CI ACNGT 2 cut(s) 407, 421
Bst6I CTCTTC 2 cut(s) 81, 86
BstACI GRCGYC 1 cut(s) 354
BstDEI CTNAG 2 cut(s) 189, 303
BstF5I GGATG 1 cut(s) 341
BstKTI GATC 1 cut(s) 106
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNSI RCATGY 1 cut(s) 265
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 146, 183
BstSFI CTRYAG 1 cut(s) 546
BstSLI GKGCMC 2 cut(s) 69, 322
BstV1I GCAGC 2 cut(s) 87, 294
BstXI CCANNNNNNTGG 1 cut(s) 202
BsuRI GGCC 2 cut(s) 183, 429
BtrI CACGTC 2 cut(s) 243, 500
BtsCI GGATG 1 cut(s) 341
BtsIMutI CAGTG 1 cut(s) 106
BveI ACCTGC 1 cut(s) 244
CaiI CAGNNNCTG 1 cut(s) 142
Cfr10I RCCGGY 2 cut(s) 224, 389
Cfr13I GGNCC 4 cut(s) 17, 182, 287, 427
CspAI ACCGGT 1 cut(s) 389
CspCI CAANNNNNGTGG 2 cut(s) 186, 221
CviAII CATG 3 cut(s) 52, 262, 347
DdeI CTNAG 2 cut(s) 189, 303
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 81, 86
EarI CTCTTC 2 cut(s) 81, 86
Eco130I CCWWGG 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 17, 287
Eco88I CYCGRG 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 3 cut(s) 55, 265, 350
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 509, 541
FatI CATG 3 cut(s) 51, 261, 346
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 1 cut(s) 361
Fnu4HI GCNGC 2 cut(s) 76, 308
FokI GGATG 1 cut(s) 328
Fsp4HI GCNGC 2 cut(s) 76, 308
GluI GCNGC 2 cut(s) 76, 308
HaeIII GGCC 2 cut(s) 183, 429
HapII CCGG 6 cut(s) 148, 180, 185, 225, 390, 430
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 3 cut(s) 55, 265, 350
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 1 cut(s) 446
HpaII CCGG 6 cut(s) 148, 180, 185, 225, 390, 430
HphI GGTGA 3 cut(s) 379, 451, 494
Hpy166II GTNNAC 3 cut(s) 67, 259, 362
Hpy188I TCNGA 2 cut(s) 192, 304
Hpy8I GTNNAC 3 cut(s) 67, 259, 362
Hpy99I CGWCG 1 cut(s) 247
HpyCH4III ACNGT 2 cut(s) 407, 421
HpyCH4IV ACGT 3 cut(s) 242, 354, 499
HpyCH4V TGCA 1 cut(s) 67
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 2 cut(s) 189, 303
HpySE526I ACGT 3 cut(s) 242, 354, 499
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 3 cut(s) 55, 265, 350
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 2 cut(s) 126, 156
Lsp1109I GCAGC 2 cut(s) 87, 294
LweI GCATC 1 cut(s) 350
MaeII ACGT 3 cut(s) 242, 354, 499
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 3 cut(s) 28, 98, 103
MhlI GDGCHC 2 cut(s) 69, 322
MluCI AATT 1 cut(s) 485
MnlI CCTC 2 cut(s) 285, 343
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 1 cut(s) 200
MspI CCGG 6 cut(s) 148, 180, 185, 225, 390, 430
MspR9I CCNGG 2 cut(s) 148, 185
Mva1269I GAATGC 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 148
NciI CCSGG 2 cut(s) 148, 185
NdeII GATC 1 cut(s) 103
NlaIII CATG 3 cut(s) 55, 265, 350
NlaIV GGNNCC 3 cut(s) 122, 152, 210
NspI RCATGY 1 cut(s) 265
OliI CACNNNNGTG 1 cut(s) 200
PciI ACATGT 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 496
PctI GAATGC 1 cut(s) 439
PfeI GAWTC 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 389
PkrI GCNGC 2 cut(s) 77, 309
PpuMI RGGWCCY 1 cut(s) 287
PscI ACATGT 1 cut(s) 261
PshAI GACNNNNGTC 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 287
PspN4I GGNNCC 3 cut(s) 122, 152, 210
PspPI GGNCC 4 cut(s) 17, 182, 287, 427
PspPPI RGGWCCY 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 2 cut(s) 76, 308
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 4 cut(s) 17, 182, 287, 427
ScrFI CCNGG 2 cut(s) 148, 185
SduI GDGCHC 2 cut(s) 69, 322
SfaNI GCATC 1 cut(s) 350
SfcI CTRYAG 1 cut(s) 546
SgrAI CRCCGGYG 1 cut(s) 389
SinI GGWCC 2 cut(s) 17, 287
SmiMI CAYNNNNRTG 1 cut(s) 200
Sse9I AATT 1 cut(s) 485
SsiI CCGC 1 cut(s) 437
StyD4I CCNGG 2 cut(s) 146, 183
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 2 cut(s) 407, 421
TaiI ACGT 3 cut(s) 245, 357, 502
TaqI TCGA 1 cut(s) 490
TasI AATT 1 cut(s) 485
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 113
TseI GCWGC 2 cut(s) 75, 307
TspDTI ATGAA 1 cut(s) 40
TspRI CASTG 1 cut(s) 113
VneI GTGCAC 1 cut(s) 65
VpaK11BI GGWCC 2 cut(s) 17, 287
XapI RAATTY 1 cut(s) 485
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 2 cut(s) 202, 329
XmiI GTMKAC 1 cut(s) 361
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.