Rroxscaffold_7G00215760

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006047
Physical Location & Seq
Forward (+)
66324620 .. 66325851
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215760.1

Sequence Viewer

Length: 600 bp
ATGTTTGACAACCCACTAACCAAGGGACCCGAACTCACGTCCGAGCTTGTAGGTAGGGCTCAGGGGCTATACAGTTTCGCGTCCCAAGAAGAGCTTTGTCTACTTATGGCCATGACCATTGTTTTCACCAGGGGCAAACACAACGGTAGCGGTGTCACTATTTTGGGGCGCAACGCGGTGTTTCAATCCCGGCGAGAGTTTCCAATTGTCGGCGGAACTGGCGATTTCCGGTTGGCCCGTGGATTTGCGTCAGTCTCCAGCTATTTTGTTAATGCTACTCTTGGTATTCTGGAATATAATCTTATGGGAATTCCAAATAATGATCCAAGCATAGGAACATTCTTCACTAAATCAACGGACAGAATTCTTAGAAAACCTGAACAAGGCTTCCAACATCTAATTGAAGCACAACTCTTAAATCTGCTCCGGAAAGAAAAACATGCTATACAATCACAAGAAAAAGATCACATAGTTATTTGCCATCACCCAGGCAAAGGGATTTCCCTTTGTGAGAAGCAGCAAAAGTCCAGGATGATGGATTCGAAAAACAGAAGATTATTAAGCTTGTTCCTTTCAAAACACCTGCAATTCAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

199

Amino Acids

22.38

Weight (kDa)

9.63

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 1 - 101 2.2e-35 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 591
Acc36I ACCTGC 1 cut(s) 591
AccI GTMKAC 1 cut(s) 100
AccII CGCG 2 cut(s) 80, 176
AccIII TCCGGA 1 cut(s) 426
AciI CCGC 3 cut(s) 150, 176, 213
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 2 cut(s) 309, 363
AfiI CCNNNNNNNGG 4 cut(s) 209, 332, 383, 494
AgsI TTSAA 4 cut(s) 185, 404, 576, 592
AjiI CACGTC 1 cut(s) 39
AjnI CCWGG 3 cut(s) 128, 487, 527
AloI GAACNNNNNNTCC 2 cut(s) 372, 404
AluBI AGCT 4 cut(s) 46, 94, 261, 564
AluI AGCT 4 cut(s) 46, 94, 261, 564
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 1 cut(s) 317
Aor13HI TCCGGA 1 cut(s) 426
AoxI GGCC 2 cut(s) 108, 234
ApeKI GCWGC 1 cut(s) 517
ApoI RAATTY 2 cut(s) 309, 363
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 2 cut(s) 26, 235
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 2 cut(s) 118, 476
AsuII TTCGAA 1 cut(s) 542
AvaII GGWCC 1 cut(s) 26
BalI TGGCCA 1 cut(s) 110
BanII GRGCYC 1 cut(s) 61
BbvI GCAGC 1 cut(s) 529
BccI CCATC 2 cut(s) 489, 529
BciT130I CCWGG 3 cut(s) 130, 489, 529
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 1 cut(s) 598
BfuAI ACCTGC 1 cut(s) 591
BisI GCNGC 1 cut(s) 518
BlsI GCNGC 1 cut(s) 519
Bme1390I CCNGG 4 cut(s) 130, 190, 489, 529
Bme18I GGWCC 1 cut(s) 26
BmgBI CACGTC 1 cut(s) 39
BmgT120I GGNCC 2 cut(s) 26, 235
BmiI GGNNCC 2 cut(s) 27, 28
BmrFI CCNGG 4 cut(s) 130, 190, 489, 529
BpmI CTGGAG 1 cut(s) 241
Bpu10I CCTNAGC 1 cut(s) 60
Bpu14I TTCGAA 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 190
BsaJI CCNNGG 4 cut(s) 21, 129, 238, 487
BsaWI WCCGGW 2 cut(s) 228, 426
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 332, 383, 494
Bse1I ACTGG 1 cut(s) 223
BseAI TCCGGA 1 cut(s) 426
BseBI CCWGG 3 cut(s) 130, 489, 529
BseDI CCNNGG 4 cut(s) 21, 129, 238, 487
BseGI GGATG 1 cut(s) 537
BseLI CCNNNNNNNGG 4 cut(s) 209, 332, 383, 494
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 223
BseXI GCAGC 1 cut(s) 529
Bsh1236I CGCG 2 cut(s) 80, 176
BshFI GGCC 2 cut(s) 110, 236
BsiSI CCGG 3 cut(s) 190, 229, 427
BslFI GGGAC 2 cut(s) 39, 67
BslI CCNNNNNNNGG 4 cut(s) 209, 332, 383, 494
BsmAI GTCTC 1 cut(s) 259
BsmFI GGGAC 2 cut(s) 39, 67
BsnI GGCC 2 cut(s) 110, 236
Bsp119I TTCGAA 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 61
Bsp13I TCCGGA 1 cut(s) 426
Bsp143I GATC 2 cut(s) 322, 463
BspACI CCGC 3 cut(s) 150, 176, 213
BspANI GGCC 2 cut(s) 110, 236
BspCNI CTCAG 1 cut(s) 73
BspEI TCCGGA 1 cut(s) 426
BspFNI CGCG 2 cut(s) 80, 176
BspLI GGNNCC 2 cut(s) 27, 28
BspMI ACCTGC 1 cut(s) 591
BspPI GGATC 1 cut(s) 317
BspQI GCTCTTC 1 cut(s) 84
BspT104I TTCGAA 1 cut(s) 542
BsrI ACTGG 1 cut(s) 223
BssECI CCNNGG 4 cut(s) 21, 129, 238, 487
BssMI GATC 2 cut(s) 322, 463
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 3 cut(s) 130, 489, 529
Bst4CI ACNGT 2 cut(s) 74, 146
Bst6I CTCTTC 1 cut(s) 84
BstBI TTCGAA 1 cut(s) 542
BstDEI CTNAG 2 cut(s) 60, 368
BstDSI CCRYGG 1 cut(s) 238
BstENI CCTNNNNNAGG 1 cut(s) 381
BstF5I GGATG 1 cut(s) 537
BstFNI CGCG 2 cut(s) 80, 176
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 2 cut(s) 325, 466
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 2 cut(s) 322, 463
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstNI CCWGG 3 cut(s) 130, 489, 529
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 4 cut(s) 128, 188, 487, 527
BstUI CGCG 2 cut(s) 80, 176
BstV1I GCAGC 1 cut(s) 529
BstXI CCANNNNNNTGG 1 cut(s) 535
BsuRI GGCC 2 cut(s) 110, 236
BtgI CCRYGG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 39
BtsCI GGATG 1 cut(s) 537
BveI ACCTGC 1 cut(s) 591
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 2 cut(s) 26, 235
CseI GACGC 2 cut(s) 69, 237
CviAII CATG 2 cut(s) 112, 440
CviJI RGCY 9 cut(s) 46, 59, 67, 94, 110, 236, 261, 387, 564
CviKI_1 RGCY 9 cut(s) 46, 59, 67, 94, 110, 236, 261, 387, 564
DdeI CTNAG 2 cut(s) 60, 368
DpnI GATC 2 cut(s) 324, 465
DpnII GATC 2 cut(s) 322, 463
EaeI YGGCCR 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 61
Eco47I GGWCC 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 26
EcoRI GAATTC 2 cut(s) 309, 363
EcoRII CCWGG 3 cut(s) 128, 487, 527
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 2 cut(s) 115, 443
FaiI YATR 9 cut(s) 70, 107, 113, 297, 305, 332, 441, 446, 470
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FaqI GGGAC 2 cut(s) 39, 67
FatI CATG 2 cut(s) 111, 439
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 1 cut(s) 518
FokI GGATG 1 cut(s) 544
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 1 cut(s) 518
FspBI CTAG 1 cut(s) 598
GlaI GCGC 1 cut(s) 170
GluI GCNGC 1 cut(s) 518
GsuI CTGGAG 1 cut(s) 241
HaeIII GGCC 2 cut(s) 110, 236
HapII CCGG 3 cut(s) 190, 229, 427
HgaI GACGC 2 cut(s) 69, 237
HhaI GCGC 1 cut(s) 171
Hin1II CATG 2 cut(s) 115, 443
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 562
HinfI GANTC 1 cut(s) 539
HpaII CCGG 3 cut(s) 190, 229, 427
HphI GGTGA 2 cut(s) 118, 476
Hpy166II GTNNAC 1 cut(s) 101
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 2 cut(s) 290, 427
Hpy8I GTNNAC 1 cut(s) 101
HpyCH4III ACNGT 2 cut(s) 74, 146
HpyCH4IV ACGT 1 cut(s) 38
HpyCH4V TGCA 1 cut(s) 586
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
HpyF3I CTNAG 2 cut(s) 60, 368
HpySE526I ACGT 1 cut(s) 38
Hsp92II CATG 2 cut(s) 115, 443
HspAI GCGC 1 cut(s) 169
KflI GGGWCCC 1 cut(s) 26
Kpn2I TCCGGA 1 cut(s) 426
Kzo9I GATC 2 cut(s) 322, 463
LguI GCTCTTC 1 cut(s) 84
LmnI GCTCC 1 cut(s) 429
Lsp1109I GCAGC 1 cut(s) 529
MaeI CTAG 1 cut(s) 598
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 154
MalI GATC 2 cut(s) 324, 465
MboI GATC 2 cut(s) 322, 463
MboII GAAGA 3 cut(s) 101, 334, 564
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 61
MlsI TGGCCA 1 cut(s) 110
MluCI AATT 5 cut(s) 204, 309, 363, 399, 587
MluNI TGGCCA 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 415
Mox20I TGGCCA 1 cut(s) 110
MroI TCCGGA 1 cut(s) 426
MscI TGGCCA 1 cut(s) 110
MseI TTAA 3 cut(s) 270, 416, 560
Msp20I TGGCCA 1 cut(s) 110
MspI CCGG 3 cut(s) 190, 229, 427
MspR9I CCNGG 4 cut(s) 130, 190, 489, 529
MunI CAATTG 1 cut(s) 204
MvaI CCWGG 3 cut(s) 130, 489, 529
MvnI CGCG 2 cut(s) 80, 176
MwoI GCNNNNNNNGC 1 cut(s) 219
NciI CCSGG 1 cut(s) 190
NdeII GATC 2 cut(s) 322, 463
NlaIII CATG 2 cut(s) 115, 443
NlaIV GGNNCC 2 cut(s) 27, 28
NmuCI GTSAC 1 cut(s) 154
NspI RCATGY 1 cut(s) 443
NspV TTCGAA 1 cut(s) 542
PaqCI CACCTGC 1 cut(s) 591
PciSI GCTCTTC 1 cut(s) 84
PfeI GAWTC 1 cut(s) 539
PfoI TCCNGGA 1 cut(s) 527
PkrI GCNGC 1 cut(s) 519
PpuMI RGGWCCY 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 3 cut(s) 128, 487, 527
PspGI CCWGG 3 cut(s) 128, 487, 527
PspN4I GGNNCC 2 cut(s) 27, 28
PspPI GGNCC 2 cut(s) 26, 235
PspPPI RGGWCCY 1 cut(s) 26
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 3 cut(s) 270, 416, 560
SatI GCNGC 1 cut(s) 518
Sau3AI GATC 2 cut(s) 322, 463
Sau96I GGNCC 2 cut(s) 26, 235
ScrFI CCNGG 4 cut(s) 130, 190, 489, 529
SduI GDGCHC 1 cut(s) 61
SetI ASST 8 cut(s) 41, 48, 55, 96, 263, 379, 566, 585
SfuI TTCGAA 1 cut(s) 542
SinI GGWCC 1 cut(s) 26
Sse9I AATT 5 cut(s) 204, 309, 363, 399, 587
SsiI CCGC 3 cut(s) 150, 176, 213
SspMI CTAG 1 cut(s) 598
StyD4I CCNGG 4 cut(s) 128, 188, 487, 527
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 74, 146
TaiI ACGT 1 cut(s) 41
TaqI TCGA 1 cut(s) 542
TasI AATT 5 cut(s) 204, 309, 363, 399, 587
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 3 cut(s) 270, 416, 560
Tru9I TTAA 3 cut(s) 270, 416, 560
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 1 cut(s) 517
Tsp45I GTSAC 1 cut(s) 154
TspGWI ACGGA 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 26
XagI CCTNNNNNAGG 1 cut(s) 381
XapI RAATTY 2 cut(s) 309, 363
XceI RCATGY 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 100
XspI CTAG 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.