MD05G1117600.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006047
Physical Location & Seq
Forward (+)
23869544 .. 23870125
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1117600.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCAAGGGTCGGTACAATAATATTTCTCAACCTTTTGTTCATAGCATGCTTGTCCACGCCATTAATGGTACAGAGCGCCATTGAAAACCCTAGGGAGAACATTGACCAATGGTATCAAAACCTCTCCCATGCACAACAGAAGCTGACCATGCTGCACTTCTACTTGCACGAGGTCGTTTTCGGCCCGCACCCGACCTTGGTGACGGTGGCCAACGCATCCAACACCTCCACATCACTACCCTACTTCGGCCTAGTCAACATCTTCGACAACCCCTTGACCAAGGGACCCAAACCCGGTTCCAAGCTGGTGGGTCGGGCGCAAGGGCTATATGGGTTTAGCTCACAGGAGGAAGTGAGCCTGCTTACGGCTATGAACTTTGTTTTCACGAGTGGGAAGCACAGCGGCAGCAGTCTCACCGTTTTGGGGCGGAACCCGATTGGGCAGTCGGTAAGAGAGTTGCCCATCGTCGCCGGAACCGGTGTTTTCCGGTTGGCGCGTGGATTTGCATTGGTCAAGACTTATTTCCTTGATACTAGTGGTGCCATTGTTGAATATAATGTTACAATTTTGCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

194

Amino Acids

21.13

Weight (kDa)

9.1

Isoelectric Point (pI)

29.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 50 - 190 6.1e-51 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 542
AccII CGCG 1 cut(s) 499
AciI CCGC 3 cut(s) 188, 405, 430
AcoI YGGCCR 1 cut(s) 210
AfaI GTAC 2 cut(s) 16, 72
AfiI CCNNNNNNNGG 5 cut(s) 199, 248, 296, 367, 426
AgeI ACCGGT 1 cut(s) 479
AgsI TTSAA 2 cut(s) 86, 554
AhlI ACTAGT 1 cut(s) 536
AluBI AGCT 3 cut(s) 145, 307, 342
AluI AGCT 3 cut(s) 145, 307, 342
Alw26I GTCTC 1 cut(s) 419
AlwNI CAGNNNCTG 1 cut(s) 145
AoxI GGCC 3 cut(s) 184, 210, 250
ApeKI GCWGC 2 cut(s) 154, 408
AseI ATTAAT 1 cut(s) 65
AsiGI ACCGGT 1 cut(s) 479
AspA2I CCTAGG 1 cut(s) 92
AspLEI GCGC 3 cut(s) 80, 322, 499
AspS9I GGNCC 2 cut(s) 185, 287
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 2 cut(s) 214, 409
AvaII GGWCC 1 cut(s) 287
AvrII CCTAGG 1 cut(s) 92
BalI TGGCCA 1 cut(s) 212
BanI GGYRCC 1 cut(s) 542
BauI CACGAG 2 cut(s) 170, 388
BbvI GCAGC 2 cut(s) 141, 420
BccI CCATC 1 cut(s) 473
BceAI ACGGC 1 cut(s) 384
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 419
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 3 cut(s) 93, 254, 537
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 3 cut(s) 155, 406, 409
BlnI CCTAGG 1 cut(s) 92
BlsI GCNGC 3 cut(s) 156, 407, 410
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 287
BmgT120I GGNCC 2 cut(s) 185, 287
BmiI GGNNCC 6 cut(s) 288, 289, 301, 434, 478, 544
BmrFI CCNGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 297
BsaJI CCNNGG 3 cut(s) 92, 198, 282
BsaWI WCCGGW 2 cut(s) 479, 489
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 248, 296, 367, 426
Bse118I RCCGGY 1 cut(s) 479
BseDI CCNNGG 3 cut(s) 92, 198, 282
BseGI GGATG 1 cut(s) 218
BseLI CCNNNNNNNGG 5 cut(s) 199, 248, 296, 367, 426
BseXI GCAGC 2 cut(s) 141, 420
BsgI GTGCAG 1 cut(s) 140
Bsh1236I CGCG 1 cut(s) 499
BshFI GGCC 3 cut(s) 186, 212, 252
BshNI GGYRCC 1 cut(s) 542
BshTI ACCGGT 1 cut(s) 479
BsiSI CCGG 4 cut(s) 297, 474, 480, 490
BslFI GGGAC 1 cut(s) 300
BslI CCNNNNNNNGG 5 cut(s) 199, 248, 296, 367, 426
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 300
BsnI GGCC 3 cut(s) 186, 212, 252
BspACI CCGC 3 cut(s) 188, 405, 430
BspANI GGCC 3 cut(s) 186, 212, 252
BspFNI CGCG 1 cut(s) 499
BspLI GGNNCC 6 cut(s) 288, 289, 301, 434, 478, 544
BspT107I GGYRCC 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 479
BssAI RCCGGY 1 cut(s) 479
BssECI CCNNGG 3 cut(s) 92, 198, 282
BssSI CACGAG 2 cut(s) 170, 388
BssT1I CCWWGG 3 cut(s) 92, 198, 282
Bst2BI CACGAG 2 cut(s) 170, 388
Bst4CI ACNGT 2 cut(s) 208, 421
BstC8I GCNNGC 3 cut(s) 49, 188, 362
BstF5I GGATG 1 cut(s) 218
BstFNI CGCG 1 cut(s) 499
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 3 cut(s) 80, 322, 499
BstMAI GTCTC 1 cut(s) 419
BstMWI GCNNNNNNNGC 1 cut(s) 151
BstNSI RCATGY 1 cut(s) 51
BstSCI CCNGG 1 cut(s) 295
BstUI CGCG 1 cut(s) 499
BstV1I GCAGC 2 cut(s) 141, 420
BstXI CCANNNNNNTGG 1 cut(s) 310
BsuRI GGCC 3 cut(s) 186, 212, 252
BtsCI GGATG 1 cut(s) 218
Cac8I GCNNGC 3 cut(s) 49, 188, 362
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 3 cut(s) 80, 322, 499
Cfr10I RCCGGY 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 185, 287
Csp6I GTAC 2 cut(s) 15, 71
CspAI ACCGGT 1 cut(s) 479
CviAII CATG 3 cut(s) 48, 131, 151
CviJI RGCY 9 cut(s) 145, 186, 212, 252, 307, 328, 342, 360, 371
CviKI_1 RGCY 9 cut(s) 145, 186, 212, 252, 307, 328, 342, 360, 371
CviQI GTAC 2 cut(s) 15, 71
EaeI YGGCCR 1 cut(s) 210
EciI GGCGGA 1 cut(s) 445
Eco130I CCWWGG 3 cut(s) 92, 198, 282
Eco47I GGWCC 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 287
EcoT14I CCWWGG 3 cut(s) 92, 198, 282
ErhI CCWWGG 3 cut(s) 92, 198, 282
FaeI CATG 3 cut(s) 51, 134, 154
FaiI YATR 8 cut(s) 44, 49, 132, 152, 331, 333, 374, 558
FaqI GGGAC 1 cut(s) 300
FatI CATG 3 cut(s) 47, 130, 150
FauI CCCGC 1 cut(s) 195
Fnu4HI GCNGC 3 cut(s) 155, 406, 409
FokI GGATG 1 cut(s) 205
Fsp4HI GCNGC 3 cut(s) 155, 406, 409
FspBI CTAG 3 cut(s) 93, 254, 537
GlaI GCGC 3 cut(s) 79, 321, 498
GluI GCNGC 3 cut(s) 155, 406, 409
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 3 cut(s) 186, 212, 252
HapII CCGG 4 cut(s) 297, 474, 480, 490
HhaI GCGC 3 cut(s) 80, 322, 499
Hin1II CATG 3 cut(s) 51, 134, 154
Hin6I GCGC 3 cut(s) 78, 320, 497
HinP1I GCGC 3 cut(s) 78, 320, 497
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HpaII CCGG 4 cut(s) 297, 474, 480, 490
HphI GGTGA 2 cut(s) 214, 409
Hpy166II GTNNAC 2 cut(s) 57, 259
Hpy188III TCNNGA 2 cut(s) 388, 517
Hpy8I GTNNAC 2 cut(s) 57, 259
Hpy99I CGWCG 1 cut(s) 473
HpyCH4III ACNGT 2 cut(s) 208, 421
HpyCH4V TGCA 5 cut(s) 134, 157, 169, 509, 574
HpyF10VI GCNNNNNNNGC 1 cut(s) 151
Hsp92II CATG 3 cut(s) 51, 134, 154
HspAI GCGC 3 cut(s) 78, 320, 497
KflI GGGWCCC 1 cut(s) 287
LpnPI CCDG 7 cut(s) 293, 310, 332, 374, 487, 493, 503
Lsp1109I GCAGC 2 cut(s) 141, 420
LweI GCATC 1 cut(s) 227
MaeI CTAG 3 cut(s) 93, 254, 537
MaeIII GTNAC 2 cut(s) 202, 562
MboII GAAGA 1 cut(s) 256
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 1 cut(s) 567
MluNI TGGCCA 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 246
MnlI CCTC 4 cut(s) 134, 166, 238, 343
Mox20I TGGCCA 1 cut(s) 212
MscI TGGCCA 1 cut(s) 212
MseI TTAA 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 212
MspA1I CMGCKG 1 cut(s) 405
MspI CCGG 4 cut(s) 297, 474, 480, 490
MspR9I CCNGG 1 cut(s) 297
MvnI CGCG 1 cut(s) 499
MwoI GCNNNNNNNGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 297
NlaIII CATG 3 cut(s) 51, 134, 154
NlaIV GGNNCC 6 cut(s) 288, 289, 301, 434, 478, 544
NmuCI GTSAC 1 cut(s) 202
NspI RCATGY 1 cut(s) 51
PaeI GCATGC 1 cut(s) 51
PinAI ACCGGT 1 cut(s) 479
PkrI GCNGC 3 cut(s) 156, 407, 410
PpuMI RGGWCCY 1 cut(s) 287
PshBI ATTAAT 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 287
PspN4I GGNNCC 6 cut(s) 288, 289, 301, 434, 478, 544
PspPI GGNCC 2 cut(s) 185, 287
PspPPI RGGWCCY 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 145
RsaI GTAC 2 cut(s) 16, 72
RsaNI GTAC 2 cut(s) 15, 71
SaqAI TTAA 1 cut(s) 65
SatI GCNGC 3 cut(s) 155, 406, 409
Sau96I GGNCC 2 cut(s) 185, 287
ScrFI CCNGG 1 cut(s) 297
SetI ASST 8 cut(s) 36, 126, 147, 177, 200, 230, 309, 344
SfaNI GCATC 1 cut(s) 227
SinI GGWCC 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 536
SphI GCATGC 1 cut(s) 51
Sse9I AATT 1 cut(s) 567
SsiI CCGC 3 cut(s) 188, 405, 430
SspI AATATT 1 cut(s) 24
SspMI CTAG 3 cut(s) 93, 254, 537
StyD4I CCNGG 1 cut(s) 295
StyI CCWWGG 3 cut(s) 92, 198, 282
TaaI ACNGT 2 cut(s) 208, 421
TaqI TCGA 1 cut(s) 267
TasI AATT 1 cut(s) 567
TauI GCSGC 1 cut(s) 408
Tru1I TTAA 1 cut(s) 65
Tru9I TTAA 1 cut(s) 65
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 2 cut(s) 154, 408
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 31, 389
VpaK11BI GGWCC 1 cut(s) 287
VspI ATTAAT 1 cut(s) 65
XceI RCATGY 1 cut(s) 51
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XmaJI CCTAGG 1 cut(s) 92
XspI CTAG 3 cut(s) 93, 254, 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.