RLG00000015348

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006047
Physical Location & Seq
Forward (+)
64851526 .. 64853058
1533 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015348

Sequence Viewer

Length: 531 bp
ATGTTTGCCACATCTGTGCTATTGTATTTGACAGATTTGGGACACAGCAGGACAAGAAAGGTAGGTCTAGCTGACACTGAGGCATTTCCATTTATACTACTCGGCAACAAAATTGATATAGATGGCGGAAACATCAGAGCGACATCAGTCATAGCGGTCCCACGACCCAAGACCATCAAACCATCCACTACTATGTTCGGACAAGTCAGTGTGTTTGACAACCCGCTAACCAAGGGACCCGAACTCACATCCGAGCTTGAAGGCAGGGCTCAGGGGCTATACAGTTACGCGTCCCAAGAAGAATTCAGTCTACTTCTGGCCATGACCATTGTTTTCACCGGCGGCAAAAACAACGGTAGCAGTGTCACTATTTTGGGGCGCAACTCAATGTTTCAATCCCGGCGAGAGTTTCCACTTGTCGGTGGAACTGGTGATTTCCGGTTGGTTCGTGGATTTGCGTCGGCCTCGACCTATATTGCTAATGCCAGTCTTGCTACTGTGGAATATAATGTTACGGTTATACATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

177

Amino Acids

19.04

Weight (kDa)

8.96

Isoelectric Point (pI)

34.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 46 - 173 1.9e-41 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 310
AccII CGCG 1 cut(s) 290
AciI CCGC 4 cut(s) 126, 155, 224, 342
AcoI YGGCCR 1 cut(s) 318
AcsI RAATTY 1 cut(s) 302
AfiI CCNNNNNNNGG 1 cut(s) 419
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 2 cut(s) 260, 395
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 2 cut(s) 71, 256
AluI AGCT 2 cut(s) 71, 256
AoxI GGCC 2 cut(s) 318, 462
ApoI RAATTY 1 cut(s) 302
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 2 cut(s) 157, 236
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 2 cut(s) 328, 443
AvaII GGWCC 2 cut(s) 157, 236
BalI TGGCCA 1 cut(s) 320
BanII GRGCYC 1 cut(s) 271
BccI CCATC 3 cut(s) 116, 182, 190
BcnI CCSGG 1 cut(s) 400
BfaI CTAG 1 cut(s) 68
BisI GCNGC 1 cut(s) 343
BlsI GCNGC 1 cut(s) 344
Bme1390I CCNGG 1 cut(s) 400
Bme18I GGWCC 2 cut(s) 157, 236
BmgT120I GGNCC 2 cut(s) 157, 236
BmiI GGNNCC 3 cut(s) 159, 237, 238
BmrFI CCNGG 1 cut(s) 400
BoxI GACNNNNGTC 1 cut(s) 146
Bpu10I CCTNAGC 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 400
BsaJI CCNNGG 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 438
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 2 cut(s) 433, 486
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 2 cut(s) 182, 248
BseLI CCNNNNNNNGG 1 cut(s) 419
BseMII CTCAG 2 cut(s) 69, 284
BseNI ACTGG 2 cut(s) 433, 486
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 2 cut(s) 320, 464
BsiSI CCGG 3 cut(s) 339, 400, 439
BslFI GGGAC 4 cut(s) 54, 143, 249, 277
BslI CCNNNNNNNGG 1 cut(s) 419
BsmFI GGGAC 4 cut(s) 54, 143, 249, 277
BsnI GGCC 2 cut(s) 320, 464
Bsp1286I GDGCHC 1 cut(s) 271
BspACI CCGC 4 cut(s) 126, 155, 224, 342
BspANI GGCC 2 cut(s) 320, 464
BspCNI CTCAG 2 cut(s) 70, 283
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 3 cut(s) 159, 237, 238
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 2 cut(s) 433, 486
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 1 cut(s) 231
BssT1I CCWWGG 1 cut(s) 231
Bst4CI ACNGT 4 cut(s) 284, 356, 499, 517
BstDEI CTNAG 2 cut(s) 78, 270
BstF5I GGATG 2 cut(s) 182, 248
BstFNI CGCG 1 cut(s) 290
BstHHI GCGC 1 cut(s) 381
BstMWI GCNNNNNNNGC 1 cut(s) 491
BstPAI GACNNNNGTC 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 398
BstUI CGCG 1 cut(s) 290
BsuRI GGCC 2 cut(s) 320, 464
BtsCI GGATG 2 cut(s) 182, 248
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 3 cut(s) 75, 214, 367
CfoI GCGC 1 cut(s) 381
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 157, 236
CseI GACGC 2 cut(s) 279, 447
CviAII CATG 1 cut(s) 322
CviJI RGCY 6 cut(s) 71, 256, 269, 277, 320, 464
CviKI_1 RGCY 6 cut(s) 71, 256, 269, 277, 320, 464
DdeI CTNAG 2 cut(s) 78, 270
EaeI YGGCCR 1 cut(s) 318
EciI GGCGGA 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 157, 236
EcoO109I RGGNCCY 1 cut(s) 236
EcoRI GAATTC 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 231
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 1 cut(s) 325
FaiI YATR 9 cut(s) 95, 119, 152, 194, 280, 323, 474, 507, 521
FaqI GGGAC 4 cut(s) 54, 143, 249, 277
FatI CATG 1 cut(s) 321
FauI CCCGC 1 cut(s) 231
FblI GTMKAC 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 343
FokI GGATG 2 cut(s) 169, 235
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 1 cut(s) 343
FspBI CTAG 1 cut(s) 68
GlaI GCGC 1 cut(s) 380
GluI GCNGC 1 cut(s) 343
HaeIII GGCC 2 cut(s) 320, 464
HapII CCGG 3 cut(s) 339, 400, 439
HgaI GACGC 2 cut(s) 279, 447
HhaI GCGC 1 cut(s) 381
Hin1II CATG 1 cut(s) 325
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HpaII CCGG 3 cut(s) 339, 400, 439
HphI GGTGA 2 cut(s) 328, 443
Hpy166II GTNNAC 1 cut(s) 311
Hpy188I TCNGA 3 cut(s) 137, 200, 253
Hpy8I GTNNAC 1 cut(s) 311
Hpy99I CGWCG 1 cut(s) 463
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 4 cut(s) 284, 356, 499, 517
HpyF10VI GCNNNNNNNGC 1 cut(s) 491
HpyF3I CTNAG 2 cut(s) 78, 270
Hsp92II CATG 1 cut(s) 325
HspAI GCGC 1 cut(s) 379
KflI GGGWCCC 1 cut(s) 236
LpnPI CCDG 9 cut(s) 34, 250, 257, 302, 352, 413, 414, 452, 499
MaeI CTAG 1 cut(s) 68
MaeIII GTNAC 3 cut(s) 284, 364, 511
MboII GAAGA 1 cut(s) 311
MhlI GDGCHC 1 cut(s) 271
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 2 cut(s) 111, 302
MluI ACGCGT 1 cut(s) 288
MluNI TGGCCA 1 cut(s) 320
MnlI CCTC 2 cut(s) 73, 475
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MslI CAYNNNNRTG 2 cut(s) 14, 191
Msp20I TGGCCA 1 cut(s) 320
MspI CCGG 3 cut(s) 339, 400, 439
MspR9I CCNGG 1 cut(s) 400
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 1 cut(s) 491
NciI CCSGG 1 cut(s) 400
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 3 cut(s) 159, 237, 238
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 364
OliI CACNNNNGTG 1 cut(s) 14
PkrI GCNGC 1 cut(s) 344
PpuMI RGGWCCY 1 cut(s) 236
PshAI GACNNNNGTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 236
PspN4I GGNNCC 3 cut(s) 159, 237, 238
PspPI GGNCC 2 cut(s) 157, 236
PspPPI RGGWCCY 1 cut(s) 236
RseI CAYNNNNRTG 2 cut(s) 14, 191
SatI GCNGC 1 cut(s) 343
Sau96I GGNCC 2 cut(s) 157, 236
ScrFI CCNGG 1 cut(s) 400
SduI GDGCHC 1 cut(s) 271
SetI ASST 5 cut(s) 63, 67, 73, 258, 473
SgrAI CRCCGGYG 1 cut(s) 338
SinI GGWCC 2 cut(s) 157, 236
SmiMI CAYNNNNRTG 2 cut(s) 14, 191
Sse9I AATT 2 cut(s) 111, 302
SsiI CCGC 4 cut(s) 126, 155, 224, 342
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 1 cut(s) 398
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 4 cut(s) 284, 356, 499, 517
TaqI TCGA 1 cut(s) 467
TasI AATT 2 cut(s) 111, 302
TauI GCSGC 1 cut(s) 345
TscAI CASTG 3 cut(s) 82, 214, 367
TseFI GTSAC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 364
TspRI CASTG 3 cut(s) 82, 214, 367
VpaK11BI GGWCC 2 cut(s) 157, 236
XapI RAATTY 1 cut(s) 302
XmiI GTMKAC 1 cut(s) 310
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.