Rh6CG018100

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0006047
Physical Location & Seq
Reverse (-)
1827713 .. 1828351
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG018100.1

Sequence Viewer

Length: 582 bp
ATGGGGAAGGTTGTCCGTCCAGTTCTAAAGCTTTTCTTCGTAGTTCTGTTCATGTCATCATCAGTGCACAGCAGCAGCAACATTGAAGAAAAGAGAGAAAGTGATCATTGGTTTCAAAAGCTCCACTACTCAAAACAGAAGCTGACCCGACTCCATTTCTACTTTCACGACATTGTTTCAGGCCAGGCACAGACCACAGTGATGGTGGTCTCACCAGCCAAGACCGTCAAACCATCTCCCACCTTGTTCGGGCAAGTCAACATGTTTGACAACCCGCTAACCAAGGGACCCGAACTCACGTCTAAACTTCTAGGCCGTGCTCAAGGGCTATATGGGTTCGCGTCCCAGGTAGACATCAGTCTACTAGTCGCCATGACCTTCATTGTCACTAATGGCAGGCACGACGGTAGCAGCTTTACTGTTTTGGGCCGCAACACAGTGACGGATTCCACGCGAGAGCTTCCGATTGTAGGTGGAACTGGCAAATTTCGATTAGCACGTGGATTTGCGACAGCCAAGACGTACTTTTTTAATGATACTGTTGCTATCGTGGAATATAATATTGTGGTTATACATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

193

Amino Acids

21.55

Weight (kDa)

9.8

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 49 - 190 1.1e-51 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 351, 361
AccII CGCG 2 cut(s) 341, 454
AciI CCGC 2 cut(s) 275, 430
AcsI RAATTY 1 cut(s) 485
AcvI CACGTG 1 cut(s) 500
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 2 cut(s) 249, 470
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 2 cut(s) 86, 116
AhlI ACTAGT 1 cut(s) 364
AjiI CACGTC 1 cut(s) 300
AjnI CCWGG 2 cut(s) 183, 345
AluBI AGCT 5 cut(s) 31, 121, 142, 414, 460
AluI AGCT 5 cut(s) 31, 121, 142, 414, 460
Alw21I GWGCWC 2 cut(s) 69, 322
Alw26I GTCTC 1 cut(s) 214
Alw44I GTGCAC 1 cut(s) 65
AlwNI CAGNNNCTG 1 cut(s) 142
AoxI GGCC 3 cut(s) 181, 313, 427
ApaLI GTGCAC 1 cut(s) 65
ApeKI GCWGC 3 cut(s) 72, 75, 411
ApoI RAATTY 1 cut(s) 485
AspS9I GGNCC 2 cut(s) 287, 427
AsuHPI GGTGA 1 cut(s) 204
AvaII GGWCC 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 69
BbrPI CACGTG 1 cut(s) 500
Bbv12I GWGCWC 2 cut(s) 69, 322
BbvI GCAGC 3 cut(s) 84, 87, 423
BccI CCATC 2 cut(s) 196, 241
BceAI ACGGC 1 cut(s) 300
BciT130I CCWGG 2 cut(s) 185, 347
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 214
BcuI ACTAGT 1 cut(s) 364
BfaI CTAG 2 cut(s) 311, 365
BisI GCNGC 4 cut(s) 73, 76, 412, 430
BlsI GCNGC 4 cut(s) 74, 77, 413, 431
Bme1390I CCNGG 2 cut(s) 185, 347
Bme18I GGWCC 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 300
BmgT120I GGNCC 2 cut(s) 287, 427
BmiI GGNNCC 2 cut(s) 288, 289
BmrFI CCNGG 2 cut(s) 185, 347
BoxI GACNNNNGTC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 306
BsaAI YACGTR 1 cut(s) 500
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 2 cut(s) 282, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 470
Bse1I ACTGG 2 cut(s) 20, 484
BseBI CCWGG 2 cut(s) 185, 347
BseDI CCNNGG 2 cut(s) 282, 345
BseLI CCNNNNNNNGG 2 cut(s) 249, 470
BseNI ACTGG 2 cut(s) 20, 484
BseSI GKGCMC 1 cut(s) 69
BseXI GCAGC 3 cut(s) 84, 87, 423
Bsh1236I CGCG 2 cut(s) 341, 454
BshFI GGCC 3 cut(s) 183, 315, 429
BsiHKAI GWGCWC 2 cut(s) 69, 322
BslFI GGGAC 2 cut(s) 300, 328
BslI CCNNNNNNNGG 2 cut(s) 249, 470
BsmAI GTCTC 1 cut(s) 214
BsmFI GGGAC 2 cut(s) 300, 328
BsnI GGCC 3 cut(s) 183, 315, 429
Bso31I GGTCTC 1 cut(s) 214
Bsp1286I GDGCHC 2 cut(s) 69, 322
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 2 cut(s) 275, 430
BspANI GGCC 3 cut(s) 183, 315, 429
BspFNI CGCG 2 cut(s) 341, 454
BspLI GGNNCC 2 cut(s) 288, 289
BspTNI GGTCTC 1 cut(s) 214
BsrI ACTGG 2 cut(s) 20, 484
BssECI CCNNGG 2 cut(s) 282, 345
BssMI GATC 1 cut(s) 103
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 2 cut(s) 185, 347
Bst4CI ACNGT 6 cut(s) 199, 226, 407, 421, 439, 541
BstBAI YACGTR 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 398
BstFNI CGCG 2 cut(s) 341, 454
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 1 cut(s) 214
BstMBI GATC 1 cut(s) 103
BstNI CCWGG 2 cut(s) 185, 347
BstNSI RCATGY 1 cut(s) 265
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 183, 345
BstSLI GKGCMC 1 cut(s) 69
BstUI CGCG 2 cut(s) 341, 454
BstV1I GCAGC 3 cut(s) 84, 87, 423
BstXI CCANNNNNNTGG 1 cut(s) 202
BsuRI GGCC 3 cut(s) 183, 315, 429
BtrI CACGTC 1 cut(s) 300
BtsIMutI CAGTG 3 cut(s) 69, 204, 444
Cac8I GCNNGC 1 cut(s) 398
CaiI CAGNNNCTG 1 cut(s) 142
Cfr13I GGNCC 2 cut(s) 287, 427
CseI GACGC 1 cut(s) 330
Csp6I GTAC 1 cut(s) 523
CviAII CATG 3 cut(s) 52, 262, 373
CviQI GTAC 1 cut(s) 523
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 282
Eco31I GGTCTC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 287
Eco72I CACGTG 1 cut(s) 500
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 183, 345
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 3 cut(s) 55, 265, 376
FaiI YATR 7 cut(s) 53, 263, 331, 333, 374, 558, 572
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 509, 541
FaqI GGGAC 2 cut(s) 300, 328
FatI CATG 3 cut(s) 51, 261, 372
FauI CCCGC 1 cut(s) 282
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 2 cut(s) 351, 361
Fnu4HI GCNGC 4 cut(s) 73, 76, 412, 430
Fsp4HI GCNGC 4 cut(s) 73, 76, 412, 430
FspBI CTAG 2 cut(s) 311, 365
GluI GCNGC 4 cut(s) 73, 76, 412, 430
HaeIII GGCC 3 cut(s) 183, 315, 429
HgaI GACGC 1 cut(s) 330
Hin1II CATG 3 cut(s) 55, 265, 376
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 2 cut(s) 150, 446
HphI GGTGA 1 cut(s) 204
Hpy166II GTNNAC 4 cut(s) 67, 259, 352, 362
Hpy188I TCNGA 1 cut(s) 465
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 4 cut(s) 67, 259, 352, 362
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 6 cut(s) 199, 226, 407, 421, 439, 541
HpyCH4IV ACGT 3 cut(s) 299, 499, 521
HpyCH4V TGCA 1 cut(s) 67
HpySE526I ACGT 3 cut(s) 299, 499, 521
Hsp92II CATG 3 cut(s) 55, 265, 376
KflI GGGWCCC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 126
LpnPI CCDG 9 cut(s) 33, 165, 170, 197, 228, 332, 359, 382, 465
Lsp1109I GCAGC 3 cut(s) 84, 87, 423
MaeI CTAG 2 cut(s) 311, 365
MaeII ACGT 3 cut(s) 299, 499, 521
MaeIII GTNAC 2 cut(s) 385, 439
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 2 cut(s) 28, 98
MhlI GDGCHC 2 cut(s) 69, 322
MluCI AATT 1 cut(s) 485
MlyI GAGTC 1 cut(s) 144
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 1 cut(s) 200
MspR9I CCNGG 2 cut(s) 185, 347
MvaI CCWGG 2 cut(s) 185, 347
MvnI CGCG 2 cut(s) 341, 454
NdeII GATC 1 cut(s) 103
NlaIII CATG 3 cut(s) 55, 265, 376
NlaIV GGNNCC 2 cut(s) 288, 289
NmuCI GTSAC 2 cut(s) 385, 439
NspI RCATGY 1 cut(s) 265
PciI ACATGT 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 496
PfeI GAWTC 1 cut(s) 446
PkrI GCNGC 4 cut(s) 74, 77, 413, 431
PleI GAGTC 1 cut(s) 144
PmaCI CACGTG 1 cut(s) 500
PmlI CACGTG 1 cut(s) 500
PpsI GAGTC 1 cut(s) 144
Ppu21I YACGTR 1 cut(s) 500
PpuMI RGGWCCY 1 cut(s) 287
PscI ACATGT 1 cut(s) 261
PshAI GACNNNNGTC 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 287
Psp6I CCWGG 2 cut(s) 183, 345
PspCI CACGTG 1 cut(s) 500
PspGI CCWGG 2 cut(s) 183, 345
PspN4I GGNNCC 2 cut(s) 288, 289
PspPI GGNCC 2 cut(s) 287, 427
PspPPI RGGWCCY 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 142
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
RseI CAYNNNNRTG 1 cut(s) 200
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 4 cut(s) 73, 76, 412, 430
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 2 cut(s) 287, 427
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 2 cut(s) 185, 347
SduI GDGCHC 2 cut(s) 69, 322
SinI GGWCC 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
SpeI ACTAGT 1 cut(s) 364
Sse9I AATT 1 cut(s) 485
SsiI CCGC 2 cut(s) 275, 430
SspI AATATT 1 cut(s) 562
SspMI CTAG 2 cut(s) 311, 365
StyD4I CCNGG 2 cut(s) 183, 345
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 6 cut(s) 199, 226, 407, 421, 439, 541
TaiI ACGT 3 cut(s) 302, 502, 524
TaqI TCGA 1 cut(s) 490
TasI AATT 1 cut(s) 485
TauI GCSGC 1 cut(s) 432
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 3 cut(s) 69, 204, 444
TseFI GTSAC 2 cut(s) 385, 439
TseI GCWGC 3 cut(s) 72, 75, 411
Tsp45I GTSAC 2 cut(s) 385, 439
TspDTI ATGAA 2 cut(s) 40, 370
TspGWI ACGGA 2 cut(s) 5, 458
TspRI CASTG 3 cut(s) 69, 204, 444
VneI GTGCAC 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 1 cut(s) 485
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XmiI GTMKAC 2 cut(s) 351, 361
XspI CTAG 2 cut(s) 311, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.