FvH4_7g23270

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006047
Physical Location & Seq
Reverse (-)
18182820 .. 18183371
552 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23270.t1

Sequence Viewer

Length: 405 bp
ATGGAGAAGGTGATTTTCTTCTTAGCTTTGTCCATGATCTCACCATTACTAGGGCGCAGTAGTAGCATGGAAGAAAACAGAGAATCCGACAAACAGGTCAACATCTTCCACAACCCCATAACCAAAGGACCCGAGATCACGTCGGAGCACCTAGGCATGGCTCAGAGGCTATATAGTTTCGCGTCCCAAGAAGAATTCAGTCTACTTTTGGCCATGACCATTGTTTTCACCGGCGGCCAACACAACGGTAGCAGTGTTACTATTTTGGGGCGCAATCCGGTGCTTCAGTCCCGGCGAGAGTTTCCTATCGTCGGCGGCACCGGCGATTTCCGGTTGGCCCGTGGATTTGCGACGGCCTCCAATTATAAGGCTCTTGCTAGCGTGACTGTAGTGGATATAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

135

Amino Acids

14.71

Weight (kDa)

6.83

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 32 - 124 8.7e-31 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 366
AasI GACNNNNNNGTC 1 cut(s) 95
AccB1I GGYRCC 1 cut(s) 317
AccI GTMKAC 1 cut(s) 202
AccII CGCG 1 cut(s) 182
AciI CCGC 2 cut(s) 234, 315
AcoI YGGCCR 2 cut(s) 210, 235
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 1 cut(s) 269
AfiI CCNNNNNNNGG 3 cut(s) 50, 157, 311
AjiI CACGTC 1 cut(s) 141
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
Alw21I GWGCWC 1 cut(s) 150
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 4 cut(s) 210, 235, 336, 354
ApoI RAATTY 1 cut(s) 194
AspA2I CCTAGG 1 cut(s) 151
AspLEI GCGC 2 cut(s) 57, 273
AspS9I GGNCC 2 cut(s) 128, 337
AsuC2I CCSGG 1 cut(s) 292
AsuHPI GGTGA 3 cut(s) 22, 33, 220
AsuNHI GCTAGC 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 1 cut(s) 128
AvrII CCTAGG 1 cut(s) 151
BalI TGGCCA 1 cut(s) 212
BanI GGYRCC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 150
BceAI ACGGC 1 cut(s) 369
BcnI CCSGG 1 cut(s) 292
BfaI CTAG 3 cut(s) 50, 152, 378
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 2 cut(s) 235, 316
BlnI CCTAGG 1 cut(s) 151
BlsI GCNGC 2 cut(s) 236, 317
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 131
BmgBI CACGTC 1 cut(s) 141
BmgT120I GGNCC 2 cut(s) 128, 337
BmiI GGNNCC 2 cut(s) 130, 319
BmrFI CCNGG 1 cut(s) 292
BmtI GCTAGC 1 cut(s) 381
BpuMI CCSGG 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 151, 340
BsaWI WCCGGW 2 cut(s) 277, 330
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 157, 311
Bse118I RCCGGY 2 cut(s) 230, 320
BseDI CCNNGG 2 cut(s) 151, 340
BseLI CCNNNNNNNGG 3 cut(s) 50, 157, 311
BseMII CTCAG 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 4 cut(s) 212, 237, 338, 356
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 5 cut(s) 231, 278, 292, 321, 331
BslFI GGGAC 2 cut(s) 169, 274
BslI CCNNNNNNNGG 3 cut(s) 50, 157, 311
BsmFI GGGAC 2 cut(s) 169, 274
BsnI GGCC 4 cut(s) 212, 237, 338, 356
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 2 cut(s) 36, 135
BspACI CCGC 2 cut(s) 234, 315
BspANI GGCC 4 cut(s) 212, 237, 338, 356
BspCNI CTCAG 1 cut(s) 175
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 2 cut(s) 130, 319
BspOI GCTAGC 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 317
BsrFI RCCGGY 2 cut(s) 230, 320
BssAI RCCGGY 2 cut(s) 230, 320
BssECI CCNNGG 2 cut(s) 151, 340
BssMI GATC 2 cut(s) 36, 135
BssT1I CCWWGG 1 cut(s) 151
Bst4CI ACNGT 2 cut(s) 248, 388
BstC8I GCNNGC 1 cut(s) 379
BstDEI CTNAG 2 cut(s) 22, 162
BstDSI CCRYGG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 2 cut(s) 57, 273
BstKTI GATC 2 cut(s) 39, 138
BstMBI GATC 2 cut(s) 36, 135
BstMWI GCNNNNNNNGC 2 cut(s) 63, 321
BstSCI CCNGG 1 cut(s) 290
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 1 cut(s) 182
BsuRI GGCC 4 cut(s) 212, 237, 338, 356
BtgI CCRYGG 1 cut(s) 340
BtrI CACGTC 1 cut(s) 141
BtsI GCAGTG 1 cut(s) 259
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 379
CfoI GCGC 2 cut(s) 57, 273
Cfr10I RCCGGY 2 cut(s) 230, 320
Cfr13I GGNCC 2 cut(s) 128, 337
CseI GACGC 1 cut(s) 171
CviAII CATG 4 cut(s) 34, 67, 157, 214
CviJI RGCY 8 cut(s) 26, 161, 169, 212, 237, 338, 356, 371
CviKI_1 RGCY 8 cut(s) 26, 161, 169, 212, 237, 338, 356, 371
DdeI CTNAG 2 cut(s) 22, 162
DpnI GATC 2 cut(s) 38, 137
DpnII GATC 2 cut(s) 36, 135
DrdI GACNNNNNNGTC 1 cut(s) 95
DseDI GACNNNNNNGTC 1 cut(s) 95
EaeI YGGCCR 2 cut(s) 210, 235
Eco130I CCWWGG 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 4 cut(s) 37, 70, 160, 217
FaiI YATR 9 cut(s) 35, 68, 119, 158, 172, 174, 215, 366, 398
FaqI GGGAC 2 cut(s) 169, 274
FatI CATG 4 cut(s) 33, 66, 156, 213
FblI GTMKAC 1 cut(s) 202
Fnu4HI GCNGC 2 cut(s) 235, 316
Fsp4HI GCNGC 2 cut(s) 235, 316
FspBI CTAG 3 cut(s) 50, 152, 378
GlaI GCGC 2 cut(s) 56, 272
GluI GCNGC 2 cut(s) 235, 316
HaeIII GGCC 4 cut(s) 212, 237, 338, 356
HapII CCGG 5 cut(s) 231, 278, 292, 321, 331
HgaI GACGC 1 cut(s) 171
HhaI GCGC 2 cut(s) 57, 273
Hin1II CATG 4 cut(s) 37, 70, 160, 217
Hin6I GCGC 2 cut(s) 55, 271
HinP1I GCGC 2 cut(s) 55, 271
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 1 cut(s) 83
HpaII CCGG 5 cut(s) 231, 278, 292, 321, 331
HphI GGTGA 3 cut(s) 22, 33, 220
Hpy166II GTNNAC 2 cut(s) 100, 203
Hpy188I TCNGA 3 cut(s) 88, 145, 165
Hpy8I GTNNAC 2 cut(s) 100, 203
Hpy99I CGWCG 3 cut(s) 145, 314, 355
HpyCH4III ACNGT 2 cut(s) 248, 388
HpyCH4IV ACGT 1 cut(s) 140
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 321
HpyF3I CTNAG 2 cut(s) 22, 162
HpySE526I ACGT 1 cut(s) 140
Hsp92II CATG 4 cut(s) 37, 70, 160, 217
HspAI GCGC 2 cut(s) 55, 271
Kzo9I GATC 2 cut(s) 36, 135
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 6 cut(s) 80, 244, 291, 305, 334, 344
MaeI CTAG 3 cut(s) 50, 152, 378
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 2 cut(s) 256, 382
MalI GATC 2 cut(s) 38, 137
MboI GATC 2 cut(s) 36, 135
MboII GAAGA 4 cut(s) 10, 83, 97, 203
MhlI GDGCHC 1 cut(s) 150
MlsI TGGCCA 1 cut(s) 212
MluCI AATT 2 cut(s) 194, 361
MluNI TGGCCA 1 cut(s) 212
MmeI TCCRAC 2 cut(s) 111, 123
MnlI CCTC 2 cut(s) 159, 367
Mox20I TGGCCA 1 cut(s) 212
MscI TGGCCA 1 cut(s) 212
Msp20I TGGCCA 1 cut(s) 212
MspI CCGG 5 cut(s) 231, 278, 292, 321, 331
MspR9I CCNGG 1 cut(s) 292
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 2 cut(s) 63, 321
NciI CCSGG 1 cut(s) 292
NdeII GATC 2 cut(s) 36, 135
NheI GCTAGC 1 cut(s) 377
NlaIII CATG 4 cut(s) 37, 70, 160, 217
NlaIV GGNNCC 2 cut(s) 130, 319
NmuCI GTSAC 1 cut(s) 382
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 2 cut(s) 236, 317
PpuMI RGGWCCY 1 cut(s) 128
PsiI TTATAA 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 128
PspN4I GGNNCC 2 cut(s) 130, 319
PspPI GGNCC 2 cut(s) 128, 337
PspPPI RGGWCCY 1 cut(s) 128
SatI GCNGC 2 cut(s) 235, 316
Sau3AI GATC 2 cut(s) 36, 135
Sau96I GGNCC 2 cut(s) 128, 337
ScrFI CCNGG 1 cut(s) 292
SduI GDGCHC 1 cut(s) 150
SetI ASST 5 cut(s) 12, 28, 99, 143, 153
SfcI CTRYAG 1 cut(s) 387
SgrAI CRCCGGYG 2 cut(s) 230, 320
SinI GGWCC 1 cut(s) 128
Sse9I AATT 2 cut(s) 194, 361
SsiI CCGC 2 cut(s) 234, 315
SspMI CTAG 3 cut(s) 50, 152, 378
StyD4I CCNGG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 2 cut(s) 248, 388
TaiI ACGT 1 cut(s) 143
TasI AATT 2 cut(s) 194, 361
TauI GCSGC 2 cut(s) 237, 318
TfiI GAWTC 1 cut(s) 83
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 1 cut(s) 382
Tsp45I GTSAC 1 cut(s) 382
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 1 cut(s) 194
XmaJI CCTAGG 1 cut(s) 151
XmiI GTMKAC 1 cut(s) 202
XspI CTAG 3 cut(s) 50, 152, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.