Rh6CG018200

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0006047
Physical Location & Seq
Reverse (-)
1829570 .. 1830313
744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG018200.1

Sequence Viewer

Length: 744 bp
ATGATCAACAGGCATGAGCCAACTAGTAGTTTCGTGGCCTGCATATCAGCATATGTAGTACTTTTGGGTAGTGCTTATGGTAGAAAACAAGCAGATAAGATTGATGCTTCCCCGGCCAGGCCCCCTATAAAGTGCCTCCCCAGCCTTTCATTTGCTTTGCTCACAGAGAGAAAAATGAAGAAGGTAAAGTTTTTCTTCATAGCCTTATTCATGCCATTATTCTTAGTGCACAGCAGCATCATCATTGAAGAAAATAGAGAAATTGACCGCTGGTTTCAAAAGCTCCACTACTCAAAACCGAAGCTGACCCACCTCCATTTCTACTTTCACGACACAGTTTCAGGGCCGGCCCAGACCGCAGTTATGGTAGTCCCACCACCCAAGACTGTCAAGCCATCCACCACCTTGTTTGGACAAGTCACCATGTTCGACGACCCGCTAACCAAGGGACCCGAACCCACGTCCAAGCTCTTAGGTCGAGCCCAGGGGCTATACGGGTTTGCATCCCAGGAGGAAATCAGTCTACTTATGGCTGTGACCTTTGTTCTCACTAGTGGGAAGCACAATGGCAGCGGTTTCACCATCTTGGGGCGCAATGCAGTGGAACATTCCCCGCGAGAGTTTCCGATTGTAGGTGGAACCGGAAATTTCCGATTGGCCCGAGGGTTTGCGATTGCAAAGACTTTTCTTGTTAATTCTAGTGTTGCTATTGTGGAATATAATGTTGCAATTATGCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

247

Amino Acids

27.41

Weight (kDa)

9.67

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 103 - 244 2e-53 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 523
AccII CGCG 1 cut(s) 616
AciI CCGC 5 cut(s) 268, 357, 437, 573, 614
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 1 cut(s) 646
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 3 cut(s) 117, 588, 632
AgsI TTSAA 2 cut(s) 248, 278
AhlI ACTAGT 2 cut(s) 23, 551
AjiI CACGTC 1 cut(s) 462
AjnI CCWGG 3 cut(s) 116, 483, 507
AluBI AGCT 3 cut(s) 283, 304, 469
AluI AGCT 3 cut(s) 283, 304, 469
Alw21I GWGCWC 1 cut(s) 231
Alw44I GTGCAC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 660
AoxI GGCC 6 cut(s) 36, 114, 119, 344, 348, 657
ApaLI GTGCAC 1 cut(s) 227
ApeKI GCWGC 2 cut(s) 234, 570
ApoI RAATTY 1 cut(s) 646
AspLEI GCGC 1 cut(s) 594
AspS9I GGNCC 5 cut(s) 120, 344, 349, 449, 658
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 2 cut(s) 412, 571
AvaI CYCGRG 1 cut(s) 660
AvaII GGWCC 1 cut(s) 449
BaeGI GKGCMC 1 cut(s) 231
BanII GRGCYC 1 cut(s) 484
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 246, 582
BccI CCATC 2 cut(s) 403, 590
BciT130I CCWGG 3 cut(s) 118, 485, 509
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 113
BcuI ACTAGT 2 cut(s) 23, 551
BfaI CTAG 3 cut(s) 24, 552, 699
BisI GCNGC 2 cut(s) 235, 571
BlsI GCNGC 2 cut(s) 236, 572
BmcAI AGTACT 1 cut(s) 60
Bme1390I CCNGG 4 cut(s) 113, 118, 485, 509
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 660
BmgBI CACGTC 1 cut(s) 462
BmgT120I GGNCC 5 cut(s) 120, 344, 349, 449, 658
BmiI GGNNCC 4 cut(s) 122, 450, 451, 640
BmrFI CCNGG 4 cut(s) 113, 118, 485, 509
BmsI GCATC 3 cut(s) 94, 246, 512
BpuMI CCSGG 1 cut(s) 113
BsaJI CCNNGG 6 cut(s) 111, 444, 483, 484, 507, 661
BsaWI WCCGGW 1 cut(s) 641
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 588, 632
Bse118I RCCGGY 1 cut(s) 346
Bse3DI GCAATG 1 cut(s) 601
BseBI CCWGG 3 cut(s) 118, 485, 509
BseDI CCNNGG 6 cut(s) 111, 444, 483, 484, 507, 661
BseGI GGATG 2 cut(s) 395, 503
BseLI CCNNNNNNNGG 3 cut(s) 117, 588, 632
BseMI GCAATG 1 cut(s) 601
BseSI GKGCMC 1 cut(s) 231
BseXI GCAGC 2 cut(s) 246, 582
BseYI CCCAGC 1 cut(s) 140
Bsh1236I CGCG 1 cut(s) 616
BshFI GGCC 6 cut(s) 38, 116, 121, 346, 350, 659
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 1 cut(s) 660
BsiSI CCGG 3 cut(s) 113, 347, 642
BslFI GGGAC 2 cut(s) 356, 462
BslI CCNNNNNNNGG 3 cut(s) 117, 588, 632
BsmFI GGGAC 2 cut(s) 356, 462
BsnI GGCC 6 cut(s) 38, 116, 121, 346, 350, 659
BsoBI CYCGRG 1 cut(s) 660
Bsp1286I GDGCHC 2 cut(s) 231, 484
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 5 cut(s) 268, 357, 437, 573, 614
BspANI GGCC 6 cut(s) 38, 116, 121, 346, 350, 659
BspFNI CGCG 1 cut(s) 616
BspLI GGNNCC 4 cut(s) 122, 450, 451, 640
BsrDI GCAATG 1 cut(s) 601
BsrFI RCCGGY 1 cut(s) 346
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 6 cut(s) 111, 444, 483, 484, 507, 661
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 444
Bst2UI CCWGG 3 cut(s) 118, 485, 509
Bst4CI ACNGT 2 cut(s) 337, 388
BstC8I GCNNGC 2 cut(s) 40, 348
BstDEI CTNAG 2 cut(s) 223, 472
BstF5I GGATG 2 cut(s) 395, 503
BstFNI CGCG 1 cut(s) 616
BstHHI GCGC 1 cut(s) 594
BstKTI GATC 1 cut(s) 6
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 3 cut(s) 113, 141, 356
BstNI CCWGG 3 cut(s) 118, 485, 509
BstSCI CCNGG 4 cut(s) 111, 116, 483, 507
BstSLI GKGCMC 1 cut(s) 231
BstUI CGCG 1 cut(s) 616
BstV1I GCAGC 2 cut(s) 246, 582
BsuRI GGCC 6 cut(s) 38, 116, 121, 346, 350, 659
BtrI CACGTC 1 cut(s) 462
BtsCI GGATG 2 cut(s) 395, 503
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 1 cut(s) 606
Cac8I GCNNGC 2 cut(s) 40, 348
CfoI GCGC 1 cut(s) 594
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 5 cut(s) 120, 344, 349, 449, 658
Csp6I GTAC 1 cut(s) 59
CspCI CAANNNNNGTGG 2 cut(s) 391, 426
CviAII CATG 3 cut(s) 14, 211, 424
CviQI GTAC 1 cut(s) 59
DdeI CTNAG 2 cut(s) 223, 472
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
EaeI YGGCCR 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 660
EcoO109I RGGNCCY 2 cut(s) 120, 449
EcoRII CCWGG 3 cut(s) 116, 483, 507
EcoT14I CCWWGG 1 cut(s) 444
EcoT22I ATGCAT 1 cut(s) 738
EcoT38I GRGCYC 1 cut(s) 484
ErhI CCWWGG 1 cut(s) 444
FaeI CATG 3 cut(s) 17, 214, 427
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 2 cut(s) 356, 462
FatI CATG 3 cut(s) 13, 210, 423
FauI CCCGC 2 cut(s) 444, 621
FauNDI CATATG 1 cut(s) 52
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 523
Fnu4HI GCNGC 2 cut(s) 235, 571
FokI GGATG 2 cut(s) 382, 490
FriOI GRGCYC 1 cut(s) 484
FseI GGCCGGCC 1 cut(s) 350
Fsp4HI GCNGC 2 cut(s) 235, 571
FspBI CTAG 3 cut(s) 24, 552, 699
GlaI GCGC 1 cut(s) 593
GluI GCNGC 2 cut(s) 235, 571
GsaI CCCAGC 1 cut(s) 144
HaeIII GGCC 6 cut(s) 38, 116, 121, 346, 350, 659
HapII CCGG 3 cut(s) 113, 347, 642
HhaI GCGC 1 cut(s) 594
Hin1II CATG 3 cut(s) 17, 214, 427
Hin6I GCGC 1 cut(s) 592
HinP1I GCGC 1 cut(s) 592
HpaII CCGG 3 cut(s) 113, 347, 642
HphI GGTGA 2 cut(s) 412, 571
Hpy166II GTNNAC 2 cut(s) 229, 524
Hpy188I TCNGA 2 cut(s) 627, 653
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 2 cut(s) 229, 524
Hpy99I CGWCG 1 cut(s) 434
HpyAV CCTTC 1 cut(s) 175
HpyCH4III ACNGT 2 cut(s) 337, 388
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 7 cut(s) 42, 229, 503, 599, 677, 728, 736
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 141, 356
HpyF3I CTNAG 2 cut(s) 223, 472
HpySE526I ACGT 1 cut(s) 461
Hsp92II CATG 3 cut(s) 17, 214, 427
HspAI GCGC 1 cut(s) 592
KflI GGGWCCC 1 cut(s) 449
KroI GCCGGC 1 cut(s) 346
KroNI GCCGGC 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 1 cut(s) 3
LmnI GCTCC 1 cut(s) 288
Lsp1109I GCAGC 2 cut(s) 246, 582
LweI GCATC 3 cut(s) 94, 246, 512
MaeI CTAG 3 cut(s) 24, 552, 699
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 2 cut(s) 418, 535
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 3 cut(s) 187, 190, 260
MhlI GDGCHC 2 cut(s) 231, 484
MluCI AATT 4 cut(s) 261, 646, 694, 729
MnlI CCTC 4 cut(s) 146, 323, 505, 656
Mph1103I ATGCAT 1 cut(s) 738
MroNI GCCGGC 1 cut(s) 346
MseI TTAA 1 cut(s) 693
MspA1I CMGCKG 2 cut(s) 270, 573
MspI CCGG 3 cut(s) 113, 347, 642
MspR9I CCNGG 4 cut(s) 113, 118, 485, 509
MvaI CCWGG 3 cut(s) 118, 485, 509
MvnI CGCG 1 cut(s) 616
MwoI GCNNNNNNNGC 3 cut(s) 113, 141, 356
NaeI GCCGGC 1 cut(s) 348
NciI CCSGG 1 cut(s) 113
NdeI CATATG 1 cut(s) 52
NdeII GATC 1 cut(s) 3
NgoMIV GCCGGC 1 cut(s) 346
NlaIII CATG 3 cut(s) 17, 214, 427
NlaIV GGNNCC 4 cut(s) 122, 450, 451, 640
NmuCI GTSAC 2 cut(s) 418, 535
NsiI ATGCAT 1 cut(s) 738
PasI CCCWGGG 1 cut(s) 484
PdiI GCCGGC 1 cut(s) 348
PkrI GCNGC 2 cut(s) 236, 572
PpuMI RGGWCCY 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 3 cut(s) 116, 483, 507
PspFI CCCAGC 1 cut(s) 140
PspGI CCWGG 3 cut(s) 116, 483, 507
PspN4I GGNNCC 4 cut(s) 122, 450, 451, 640
PspPI GGNCC 5 cut(s) 120, 344, 349, 449, 658
PspPPI RGGWCCY 1 cut(s) 449
RigI GGCCGGCC 1 cut(s) 350
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 2 cut(s) 235, 571
Sau3AI GATC 1 cut(s) 3
Sau96I GGNCC 5 cut(s) 120, 344, 349, 449, 658
ScaI AGTACT 1 cut(s) 60
ScrFI CCNGG 4 cut(s) 113, 118, 485, 509
SduI GDGCHC 2 cut(s) 231, 484
SfaNI GCATC 3 cut(s) 94, 246, 512
SinI GGWCC 1 cut(s) 449
SpeI ACTAGT 2 cut(s) 23, 551
Sse9I AATT 4 cut(s) 261, 646, 694, 729
SsiI CCGC 5 cut(s) 268, 357, 437, 573, 614
SspMI CTAG 3 cut(s) 24, 552, 699
StyD4I CCNGG 4 cut(s) 111, 116, 483, 507
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 2 cut(s) 337, 388
TaiI ACGT 1 cut(s) 464
TaqI TCGA 2 cut(s) 429, 478
TasI AATT 4 cut(s) 261, 646, 694, 729
TatI WGTACW 1 cut(s) 58
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TscAI CASTG 1 cut(s) 606
TseFI GTSAC 2 cut(s) 418, 535
TseI GCWGC 2 cut(s) 234, 570
Tsp45I GTSAC 2 cut(s) 418, 535
TspDTI ATGAA 4 cut(s) 138, 187, 191, 199
TspRI CASTG 1 cut(s) 606
VneI GTGCAC 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 523
XspI CTAG 3 cut(s) 24, 552, 699
ZrmI AGTACT 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.