RLG00000015345

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0006047
Physical Location & Seq
Forward (+)
64805014 .. 64805514
501 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015345

Sequence Viewer

Length: 384 bp
ATGGTAGTCCCACCTCCCAAGACTGTCAAGCCATCCACCACCTTGTTTGGACAAGTCACCATGTTTGACGACCCGCTAACCAAGGGACCCGAACCCACGTCCAAGCTCGTAGGTGGAGCCCAGGAGCTATACGGGTTTGCATCCCAAGAGGAAATCAGTCTACTTATGGCTGTGACCTTTGTTCTCAGTAGTGGGAAGCACAATGGCAGCAGTTTCACTATCTTGGGGCTCAATGCAGTGCATGGAACATTCCCAACGAGAGTTTCCGATTGTAGAATGAAAATGGCCGGTGTGGTTCGTTTTCAAATGAATGAATGTGGTTTAGTCTTTGAGTTGGTCAAGCATGTTCCTAATAGTTGCGATGAGTTAGTCCAGATATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

128

Amino Acids

13.8

Weight (kDa)

6.26

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 2 - 81 2.5e-23 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 160
AciI CCGC 1 cut(s) 74
AcoI YGGCCR 1 cut(s) 285
AgsI TTSAA 1 cut(s) 305
AjiI CACGTC 1 cut(s) 99
AjnI CCWGG 1 cut(s) 120
AjuI GAANNNNNNNTTGG 2 cut(s) 247, 279
AluBI AGCT 2 cut(s) 106, 127
AluI AGCT 2 cut(s) 106, 127
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 49
AvaII GGWCC 1 cut(s) 86
BanII GRGCYC 2 cut(s) 121, 231
BbvI GCAGC 1 cut(s) 219
BccI CCATC 1 cut(s) 40
BciT130I CCWGG 1 cut(s) 122
BisI GCNGC 1 cut(s) 208
BlsI GCNGC 1 cut(s) 209
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 86
BmgBI CACGTC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 3 cut(s) 87, 88, 118
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 81, 120
Bse118I RCCGGY 1 cut(s) 287
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 2 cut(s) 81, 120
BseGI GGATG 2 cut(s) 32, 140
BseMII CTCAG 1 cut(s) 199
BseXI GCAGC 1 cut(s) 219
BshFI GGCC 1 cut(s) 287
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 99
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 121, 231
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 87, 88, 118
BsrFI RCCGGY 1 cut(s) 287
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 2 cut(s) 81, 120
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 1 cut(s) 25
BstDEI CTNAG 1 cut(s) 185
BstF5I GGATG 2 cut(s) 32, 140
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 219
BsuRI GGCC 1 cut(s) 287
BtgZI GCGATG 1 cut(s) 375
BtrI CACGTC 1 cut(s) 99
BtsCI GGATG 2 cut(s) 32, 140
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 243
Cfr10I RCCGGY 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 86
CspCI CAANNNNNGTGG 2 cut(s) 28, 63
CviAII CATG 3 cut(s) 61, 242, 344
CviJI RGCY 7 cut(s) 31, 106, 119, 127, 170, 229, 287
CviKI_1 RGCY 7 cut(s) 31, 106, 119, 127, 170, 229, 287
DdeI CTNAG 1 cut(s) 185
EaeI YGGCCR 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 2 cut(s) 121, 231
Eco47I GGWCC 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 2 cut(s) 121, 231
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 3 cut(s) 64, 245, 347
FaiI YATR 6 cut(s) 62, 130, 167, 243, 345, 379
FaqI GGGAC 1 cut(s) 99
FatI CATG 3 cut(s) 60, 241, 343
FauI CCCGC 1 cut(s) 81
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 1 cut(s) 208
FokI GGATG 2 cut(s) 19, 127
FriOI GRGCYC 2 cut(s) 121, 231
Fsp4HI GCNGC 1 cut(s) 208
GluI GCNGC 1 cut(s) 208
HaeIII GGCC 1 cut(s) 287
HapII CCGG 1 cut(s) 288
Hin1II CATG 3 cut(s) 64, 245, 347
HpaII CCGG 1 cut(s) 288
HphI GGTGA 1 cut(s) 49
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 1 cut(s) 161
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 3 cut(s) 140, 236, 241
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 1 cut(s) 98
Hsp92II CATG 3 cut(s) 64, 245, 347
KflI GGGWCCC 1 cut(s) 86
LmnI GCTCC 2 cut(s) 116, 124
LpnPI CCDG 3 cut(s) 107, 134, 301
Lsp1109I GCAGC 1 cut(s) 219
LweI GCATC 1 cut(s) 149
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 55, 172
MhlI GDGCHC 2 cut(s) 121, 231
MnlI CCTC 2 cut(s) 24, 142
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 1 cut(s) 122
MvaI CCWGG 1 cut(s) 122
NlaIII CATG 3 cut(s) 64, 245, 347
NlaIV GGNNCC 3 cut(s) 87, 88, 118
NmuCI GTSAC 2 cut(s) 55, 172
NspI RCATGY 1 cut(s) 347
PkrI GCNGC 1 cut(s) 209
PpuMI RGGWCCY 1 cut(s) 86
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 3 cut(s) 87, 88, 118
PspPI GGNCC 1 cut(s) 86
PspPPI RGGWCCY 1 cut(s) 86
SatI GCNGC 1 cut(s) 208
Sau96I GGNCC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 2 cut(s) 121, 231
SetI ASST 7 cut(s) 16, 44, 101, 108, 115, 129, 179
SfaNI GCATC 1 cut(s) 149
SinI GGWCC 1 cut(s) 86
SsiI CCGC 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 120
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 101
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 2 cut(s) 55, 172
TseI GCWGC 1 cut(s) 207
Tsp45I GTSAC 2 cut(s) 55, 172
TspDTI ATGAA 3 cut(s) 293, 323, 327
TspRI CASTG 1 cut(s) 243
VpaK11BI GGWCC 1 cut(s) 86
XceI RCATGY 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.