Prupe.8G162200_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0006047
Physical Location & Seq
Forward (+)
16993845 .. 16994692
848 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G162200.1

Sequence Viewer

Length: 594 bp
ATGCATACACAGAGAGACACAAAAATGGGAAAGGTTGGTCCAATATTTCTAAACCTTTTGCTCTCACTATGCCTAGCCATGCCAATGGTACAAAGCACCATTGAAGGACCTGAGATTGAGCAATGGTTTCAAAACCTTCATCTAGCAAAACAGAAGCTGACCCGTCTGCACTTCTACTTCCACGACGTCGTTTTGGGCCCAAGCCAGACCTCAGTGGGGGTAGCCCGTGCACCCAACACCTTCAAATCACCCACCTTGTTCGGGCTAGTCAACATCTTTGACAACCCGCTAACCACAGGACCCGAACCCACATCTGAACTCTTGGGTCGAGCTCAAGGGCTCTACGGGTTCGCCTCCCAAGAGGAAGTGAGTCTTCTTATGTCTGTGAACTTTGTTTTCACAAGTGGCAAGCACAATGGGAGCAGCCTCACCGTTTTGGGTCGTAACCCAGTTGTGCATTCAGTCAGGGAGTTGCCAATAATCGGTGGAACTGGTGTTTTCCGATTGGCACGTGGGTTTGCGTTGGCCAAGACTTATTTTATTAATACTACTCTTGCCATTGTTGAATACAATGTTGTAGCTCTGCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

198

Amino Acids

21.67

Weight (kDa)

9.13

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AciI CCGC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 525
AcvI CACGTG 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 3 cut(s) 216, 261, 482
AgsI TTSAA 4 cut(s) 104, 131, 244, 566
AluBI AGCT 3 cut(s) 157, 332, 581
AluI AGCT 3 cut(s) 157, 332, 581
Alw21I GWGCWC 2 cut(s) 232, 334
Alw26I GTCTC 1 cut(s) 9
Alw44I GTGCAC 1 cut(s) 228
AlwNI CAGNNNCTG 1 cut(s) 157
AoxI GGCC 2 cut(s) 196, 525
ApaI GGGCCC 1 cut(s) 200
ApaLI GTGCAC 1 cut(s) 228
ApeKI GCWGC 1 cut(s) 423
AseI ATTAAT 1 cut(s) 543
AspS9I GGNCC 5 cut(s) 38, 107, 196, 197, 299
AsuHPI GGTGA 2 cut(s) 240, 421
AvaII GGWCC 3 cut(s) 38, 107, 299
BaeGI GKGCMC 2 cut(s) 200, 232
BalI TGGCCA 1 cut(s) 527
BanII GRGCYC 3 cut(s) 200, 334, 342
BbrPI CACGTG 1 cut(s) 512
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 2 cut(s) 232, 334
BbvI GCAGC 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 3 cut(s) 74, 143, 266
BisI GCNGC 1 cut(s) 424
BlsI GCNGC 1 cut(s) 425
Bme18I GGWCC 3 cut(s) 38, 107, 299
BmgT120I GGNCC 5 cut(s) 38, 107, 196, 197, 299
BmiI GGNNCC 2 cut(s) 198, 301
BmrI ACTGGG 1 cut(s) 443
BmuI ACTGGG 1 cut(s) 443
BpiI GAAGAC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 512
BsaHI GRCGYC 1 cut(s) 186
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 261, 482
Bse1I ACTGG 2 cut(s) 449, 496
Bse3DI GCAATG 1 cut(s) 128
BseLI CCNNNNNNNGG 3 cut(s) 216, 261, 482
BseMI GCAATG 1 cut(s) 128
BseMII CTCAG 2 cut(s) 102, 225
BseNI ACTGG 2 cut(s) 449, 496
BseSI GKGCMC 2 cut(s) 200, 232
BseXI GCAGC 1 cut(s) 435
BsgI GTGCAG 1 cut(s) 152
BshFI GGCC 2 cut(s) 198, 527
BsiHKAI GWGCWC 2 cut(s) 232, 334
BslI CCNNNNNNNGG 3 cut(s) 216, 261, 482
BsmAI GTCTC 1 cut(s) 9
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 2 cut(s) 198, 527
Bsp120I GGGCCC 1 cut(s) 196
Bsp1286I GDGCHC 4 cut(s) 200, 232, 334, 342
BspACI CCGC 1 cut(s) 287
BspANI GGCC 2 cut(s) 198, 527
BspCNI CTCAG 2 cut(s) 103, 224
BspLI GGNNCC 2 cut(s) 198, 301
BsrDI GCAATG 1 cut(s) 128
BsrI ACTGG 2 cut(s) 449, 496
BssNI GRCGYC 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 433
BstACI GRCGYC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 2 cut(s) 111, 211
BstMAI GTCTC 1 cut(s) 9
BstSLI GKGCMC 2 cut(s) 200, 232
BstV1I GCAGC 1 cut(s) 435
BstV2I GAAGAC 1 cut(s) 365
BstXI CCANNNNNNTGG 1 cut(s) 85
BsuRI GGCC 2 cut(s) 198, 527
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 1 cut(s) 410
CaiI CAGNNNCTG 1 cut(s) 157
Cfr13I GGNCC 5 cut(s) 38, 107, 196, 197, 299
Csp6I GTAC 1 cut(s) 89
CviAII CATG 1 cut(s) 79
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 2 cut(s) 111, 211
EaeI YGGCCR 1 cut(s) 525
Ecl136II GAGCTC 1 cut(s) 332
Eco24I GRGCYC 3 cut(s) 200, 334, 342
Eco47I GGWCC 3 cut(s) 38, 107, 299
Eco53kI GAGCTC 1 cut(s) 332
Eco72I CACGTG 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 332
EcoO109I RGGNCCY 2 cut(s) 107, 299
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 200, 334, 342
FaeI CATG 1 cut(s) 82
FaiI YATR 4 cut(s) 6, 70, 80, 380
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FatI CATG 1 cut(s) 78
FauI CCCGC 1 cut(s) 294
Fnu4HI GCNGC 1 cut(s) 424
FriOI GRGCYC 3 cut(s) 200, 334, 342
Fsp4HI GCNGC 1 cut(s) 424
FspBI CTAG 3 cut(s) 74, 143, 266
GluI GCNGC 1 cut(s) 424
HaeIII GGCC 2 cut(s) 198, 527
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 1 cut(s) 82
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 1 cut(s) 370
HphI GGTGA 2 cut(s) 240, 421
Hpy166II GTNNAC 3 cut(s) 230, 271, 388
Hpy188I TCNGA 2 cut(s) 316, 503
Hpy8I GTNNAC 3 cut(s) 230, 271, 388
Hpy99I CGWCG 2 cut(s) 188, 191
HpyAV CCTTC 3 cut(s) 98, 146, 250
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 2 cut(s) 186, 511
HpyCH4V TGCA 5 cut(s) 4, 169, 230, 457, 586
HpyF3I CTNAG 2 cut(s) 111, 211
HpySE526I ACGT 2 cut(s) 186, 511
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 1 cut(s) 82
LmnI GCTCC 1 cut(s) 420
LpnPI CCDG 6 cut(s) 123, 218, 282, 451, 462, 477
Lsp1109I GCAGC 1 cut(s) 435
MaeI CTAG 3 cut(s) 74, 143, 266
MaeII ACGT 2 cut(s) 186, 511
MaeIII GTNAC 1 cut(s) 443
MboII GAAGA 1 cut(s) 365
MhlI GDGCHC 4 cut(s) 200, 232, 334, 342
MlsI TGGCCA 1 cut(s) 527
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 4 cut(s) 220, 355, 364, 437
Mox20I TGGCCA 1 cut(s) 527
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 527
MseI TTAA 1 cut(s) 543
MslI CAYNNNNRTG 2 cut(s) 23, 83
Msp20I TGGCCA 1 cut(s) 527
Mva1269I GAATGC 1 cut(s) 457
NlaIII CATG 1 cut(s) 82
NlaIV GGNNCC 2 cut(s) 198, 301
NsiI ATGCAT 1 cut(s) 6
PctI GAATGC 1 cut(s) 457
PkrI GCNGC 1 cut(s) 425
PleI GAGTC 1 cut(s) 378
PmaCI CACGTG 1 cut(s) 512
PmlI CACGTG 1 cut(s) 512
PpsI GAGTC 1 cut(s) 378
Ppu21I YACGTR 1 cut(s) 512
PpuMI RGGWCCY 2 cut(s) 107, 299
PshBI ATTAAT 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 334
Psp5II RGGWCCY 2 cut(s) 107, 299
PspCI CACGTG 1 cut(s) 512
PspN4I GGNNCC 2 cut(s) 198, 301
PspOMI GGGCCC 1 cut(s) 196
PspPI GGNCC 5 cut(s) 38, 107, 196, 197, 299
PspPPI RGGWCCY 2 cut(s) 107, 299
PstNI CAGNNNCTG 1 cut(s) 157
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 2 cut(s) 23, 83
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 1 cut(s) 424
Sau96I GGNCC 5 cut(s) 38, 107, 196, 197, 299
SchI GAGTC 1 cut(s) 379
SduI GDGCHC 4 cut(s) 200, 232, 334, 342
SinI GGWCC 3 cut(s) 38, 107, 299
SmiMI CAYNNNNRTG 2 cut(s) 23, 83
SmlI CTYRAG 1 cut(s) 333
SmoI CTYRAG 1 cut(s) 333
SsiI CCGC 1 cut(s) 287
SspI AATATT 1 cut(s) 45
SspMI CTAG 3 cut(s) 74, 143, 266
SstI GAGCTC 1 cut(s) 334
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 2 cut(s) 189, 514
TaqI TCGA 1 cut(s) 328
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 1 cut(s) 219
TseI GCWGC 1 cut(s) 423
TspDTI ATGAA 1 cut(s) 128
TspRI CASTG 1 cut(s) 219
VneI GTGCAC 1 cut(s) 228
VpaK11BI GGWCC 3 cut(s) 38, 107, 299
VspI ATTAAT 1 cut(s) 543
XspI CTAG 3 cut(s) 74, 143, 266
ZraI GACGTC 1 cut(s) 187
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.